Frontiers in Cellular Neuroscience最新文献

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Region-independent active CNS net uptake of marketed H+/OC antiporter system substrates. 市场上销售的 H+/OC 反载体系统底物的中枢神经系统净摄取量与区域无关。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1493644
Frida Bällgren, Yang Hu, Shannuo Li, Lara van de Beek, Margareta Hammarlund-Udenaes, Irena Loryan
{"title":"Region-independent active CNS net uptake of marketed H<sup>+</sup>/OC antiporter system substrates.","authors":"Frida Bällgren, Yang Hu, Shannuo Li, Lara van de Beek, Margareta Hammarlund-Udenaes, Irena Loryan","doi":"10.3389/fncel.2024.1493644","DOIUrl":"https://doi.org/10.3389/fncel.2024.1493644","url":null,"abstract":"<p><p>The pyrilamine-sensitive proton-coupled organic cation (H<sup>+</sup>/OC) antiporter system facilitates the active net uptake of several marketed organic cationic drugs across the blood-brain barrier (BBB). This rare phenomenon has garnered interest in the H<sup>+</sup>/OC antiporter system as a potential target for CNS drug delivery. However, analysis of pharmacovigilance data has uncovered a significant association between substrates of the H<sup>+</sup>/OC antiporter and neurotoxicity, particularly drug-induced seizures (DIS) and mood- and cognitive-related adverse events (MCAEs). This preclinical study aimed to elucidate the CNS regional disposition of H<sup>+</sup>/OC antiporter substrates at therapeutically relevant plasma concentrations to uncover potential pharmacokinetic mechanisms underlying DIS and MCAEs. Here, we investigated the neuropharmacokinetics of pyrilamine, diphenhydramine, bupropion, tramadol, oxycodone, and memantine. Using the Combinatory Mapping Approach for Regions of Interest (CMA-ROI), we characterized the transport of unbound drugs across the BBB in specific CNS regions, as well as the blood-spinal cord barrier (BSCB) and the blood-cerebrospinal fluid barrier (BCSFB). Our findings demonstrated active net uptake across the BBB and BSCB, with unbound ROI-to-plasma concentration ratio, K<sub>p,uu,ROI</sub>, values consistently exceeding unity in all assessed regions. Despite minor regional differences, no significant distinctions were found when comparing the whole brain to investigated regions of interest, indicating region-independent active transport. Furthermore, we observed intracellular accumulation via lysosomal trapping for all studied drugs. These results provide new insights into the CNS regional neuropharmacokinetics of these drugs, suggesting that while the brain uptake is region-independent, the active transport mechanism enables high extracellular and intracellular drug concentrations, potentially contributing to neurotoxicity. This finding emphasizes the necessity of thorough neuropharmacokinetic evaluation and neurotoxicity profiling in the development of drugs that utilize this transport pathway.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1493644"},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global research trends and prospects on immune-related therapy in ischemic stroke: a bibliometric analysis. 缺血性中风免疫相关疗法的全球研究趋势与前景:文献计量分析。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1490607
Qi Wang, Lei Yuan, Fei Wang, Fei Sun
{"title":"Global research trends and prospects on immune-related therapy in ischemic stroke: a bibliometric analysis.","authors":"Qi Wang, Lei Yuan, Fei Wang, Fei Sun","doi":"10.3389/fncel.2024.1490607","DOIUrl":"https://doi.org/10.3389/fncel.2024.1490607","url":null,"abstract":"<p><strong>Background: </strong>Following ischemic stroke, non-neuronal cells within the nervous system play a crucial role in maintaining neurovascular unit functions, regulating metabolic and inflammatory processes of the nervous system. Investigating the functions and regulation of these cells, particularly immune cells, deepens our understanding of the complex mechanisms of neuroinflammation and immune modulation after ischemic stroke and provides new perspectives and methods for immune-related therapy.</p><p><strong>Methods: </strong>The annual distribution, journals, authors, countries, institutions, and keywords of articles published between 2015 and 2024 were visualized and analyzed using CiteSpace and other bibliometric tools.</p><p><strong>Results: </strong>A total of 1,089 relevant articles or reviews were included, demonstrating an overall upward trend; The terms \"cerebral ischemia,\" \"immune response,\" \"brain ischemia,\" \"cerebral inflammation,\" \"neurovascular unit,\" and \"immune infiltration,\" etc. are hot keywords in this field.</p><p><strong>Conclusion: </strong>In recent years, research on immune-related therapy for ischemic stroke has focused on mechanisms of occurrence, protection and repair of the blood-brain barrier (BBB) by non-neuronal cells, and regulation of immunosuppression and inflammation. Among these, reducing BBB disruption to minimize secondary brain damage has become a hotspot. At the same time, the complex roles of immune responses have attracted attention, particularly the balance between regulatory T cells and Th17 cells in regulating neuroinflammation and promoting neurological function recovery, which is crucial to reduce secondary neuronal damage and improve prognosis, potentially establishing a pivotal frontier in this domain of investigation.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1490607"},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: 15 years of frontiers in cellular neuroscience: blood brain barrier modulation and dysfunction in brain diseases. 社论:细胞神经科学前沿 15 年:脑疾病中的血脑屏障调节和功能障碍。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1511314
Duraisamy Kempuraj, Stefania Ceruti
{"title":"Editorial: 15 years of frontiers in cellular neuroscience: blood brain barrier modulation and dysfunction in brain diseases.","authors":"Duraisamy Kempuraj, Stefania Ceruti","doi":"10.3389/fncel.2024.1511314","DOIUrl":"https://doi.org/10.3389/fncel.2024.1511314","url":null,"abstract":"","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1511314"},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cholecystokinin-expressing neurons of the ventromedial hypothalamic nucleus control energy homeostasis. 下丘脑腹内侧核表达胆囊收缩素的神经元控制着能量平衡。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-28 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1483368
Vasileios Eftychidis, Tommas J Ellender, Jacek Szymanski, Liliana Minichiello
{"title":"Cholecystokinin-expressing neurons of the ventromedial hypothalamic nucleus control energy homeostasis.","authors":"Vasileios Eftychidis, Tommas J Ellender, Jacek Szymanski, Liliana Minichiello","doi":"10.3389/fncel.2024.1483368","DOIUrl":"https://doi.org/10.3389/fncel.2024.1483368","url":null,"abstract":"<p><p>The hypothalamus is the primary center of the brain that regulates energy homeostasis. The ventromedial hypothalamus (VMH) plays a central role in maintaining energy balance by regulating food intake, energy expenditure, and glucose levels. However, the cellular and molecular mechanisms underlying its functions are still poorly understood. Cholecystokinin (CCK) is one of many genes expressed in this hypothalamic nucleus. Peripheral CCK regulates food intake, body weight, and glucose homeostasis. However, current research does not explain the function of CCK neurons in specific nuclei of the hypothalamus and their likely roles in network dynamics related to energy balance and food intake. This study uses genetic and pharmacological methods to examine the role of CCK-expressing neurons in the VMH (CCK<sup>VMH</sup>). Namely, using a previously generated BAC transgenic line expressing Cre recombinase under the CCK promoter, we performed targeted manipulations of CCK<sup>VMH</sup> neurons. Histological and transcriptomic database analysis revealed extensive distribution of these neurons in the VMH, with significant heterogeneity in gene expression related to energy balance, including co-expression with PACAP and somatostatin. Pharmacogenetic acute inhibition via Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) resulted in increased food intake and altered meal patterns, characterized by higher meal frequency and shorter intermeal intervals. Furthermore, diphtheria toxin-mediated ablation of CCK<sup>VMH</sup> neurons led to significant weight gain and hyperphagia over time, increasing meal size and duration. These mice also exhibited impaired glucose tolerance, indicative of disrupted glucose homeostasis. Our findings underscore the integral role of CCK<sup>VMH</sup> neurons in modulating feeding behavior, energy homeostasis, and glucose regulation. This study enhances our understanding of the neurohormonal mechanisms underlying obesity and metabolic disorders, providing potential targets for therapeutic interventions.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1483368"},"PeriodicalIF":4.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: Novel approaches to targeting the vasculature and metabolome to prevent brain aging and related diseases. 社论:针对血管和代谢组预防大脑衰老和相关疾病的新方法。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1505939
Jennifer Ihuoma, Sharon Negri, Amanda Morato Do Canto, Anika M S Hartz, Aditi Deshpande, Stefano Tarantini
{"title":"Editorial: Novel approaches to targeting the vasculature and metabolome to prevent brain aging and related diseases.","authors":"Jennifer Ihuoma, Sharon Negri, Amanda Morato Do Canto, Anika M S Hartz, Aditi Deshpande, Stefano Tarantini","doi":"10.3389/fncel.2024.1505939","DOIUrl":"https://doi.org/10.3389/fncel.2024.1505939","url":null,"abstract":"","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1505939"},"PeriodicalIF":4.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neurovascular unit, neuroinflammation and neurodegeneration markers in brain disorders. 脑部疾病中的神经血管单元、神经炎症和神经变性标记。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1491952
Duraisamy Kempuraj, Kirk D Dourvetakis, Jessica Cohen, Daniel Seth Valladares, Rhitik Samir Joshi, Sai Puneeth Kothuru, Tristin Anderson, Baskaran Chinnappan, Amanpreet K Cheema, Nancy G Klimas, Theoharis C Theoharides
{"title":"Neurovascular unit, neuroinflammation and neurodegeneration markers in brain disorders.","authors":"Duraisamy Kempuraj, Kirk D Dourvetakis, Jessica Cohen, Daniel Seth Valladares, Rhitik Samir Joshi, Sai Puneeth Kothuru, Tristin Anderson, Baskaran Chinnappan, Amanpreet K Cheema, Nancy G Klimas, Theoharis C Theoharides","doi":"10.3389/fncel.2024.1491952","DOIUrl":"https://doi.org/10.3389/fncel.2024.1491952","url":null,"abstract":"<p><p>Neurovascular unit (NVU) inflammation via activation of glial cells and neuronal damage plays a critical role in neurodegenerative diseases. Though the exact mechanism of disease pathogenesis is not understood, certain biomarkers provide valuable insight into the disease pathogenesis, severity, progression and therapeutic efficacy. These markers can be used to assess pathophysiological status of brain cells including neurons, astrocytes, microglia, oligodendrocytes, specialized microvascular endothelial cells, pericytes, NVU, and blood-brain barrier (BBB) disruption. Damage or derangements in tight junction (TJ), adherens junction (AdJ), and gap junction (GJ) components of the BBB lead to increased permeability and neuroinflammation in various brain disorders including neurodegenerative disorders. Thus, neuroinflammatory markers can be evaluated in blood, cerebrospinal fluid (CSF), or brain tissues to determine neurological disease severity, progression, and therapeutic responsiveness. Chronic inflammation is common in age-related neurodegenerative disorders including Alzheimer's disease (AD), Parkinson's disease (PD), and dementia. Neurotrauma/traumatic brain injury (TBI) also leads to acute and chronic neuroinflammatory responses. The expression of some markers may also be altered many years or even decades before the onset of neurodegenerative disorders. In this review, we discuss markers of neuroinflammation, and neurodegeneration associated with acute and chronic brain disorders, especially those associated with neurovascular pathologies. These biomarkers can be evaluated in CSF, or brain tissues. Neurofilament light (NfL), ubiquitin C-terminal hydrolase-L1 (UCHL1), glial fibrillary acidic protein (GFAP), Ionized calcium-binding adaptor molecule 1 (Iba-1), transmembrane protein 119 (TMEM119), aquaporin, endothelin-1, and platelet-derived growth factor receptor beta (PDGFRβ) are some important neuroinflammatory markers. Recent BBB-on-a-chip modeling offers promising potential for providing an in-depth understanding of brain disorders and neurotherapeutics. Integration of these markers in clinical practice could potentially enhance early diagnosis, monitor disease progression, and improve therapeutic outcomes.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1491952"},"PeriodicalIF":4.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease. 揭示驱动蛋白-1、tau 和微管在阿尔茨海默病相关神经变性中的相互作用
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1432002
Siva Sundara Kumar Durairajan, Karthikeyan Selvarasu, Abhay Kumar Singh, Supriti Patnaik, Ashok Iyaswamy, Yogini Jaiswal, Leonard L Williams, Jian-Dong Huang
{"title":"Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease.","authors":"Siva Sundara Kumar Durairajan, Karthikeyan Selvarasu, Abhay Kumar Singh, Supriti Patnaik, Ashok Iyaswamy, Yogini Jaiswal, Leonard L Williams, Jian-Dong Huang","doi":"10.3389/fncel.2024.1432002","DOIUrl":"10.3389/fncel.2024.1432002","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is marked by the gradual and age-related deterioration of nerve cells in the central nervous system. The histopathological features observed in the brain affected by AD are the aberrant buildup of extracellular and intracellular amyloid-β and the formation of neurofibrillary tangles consisting of hyperphosphorylated tau protein. Axonal transport is a fundamental process for cargo movement along axons and relies on molecular motors like kinesins and dyneins. Kinesin's responsibility for transporting crucial cargo within neurons implicates its dysfunction in the impaired axonal transport observed in AD. Impaired axonal transport and dysfunction of molecular motor proteins, along with dysregulated signaling pathways, contribute significantly to synaptic impairment and cognitive decline in AD. Dysregulation in tau, a microtubule-associated protein, emerges as a central player, destabilizing microtubules and disrupting the transport of kinesin-1. Kinesin-1 superfamily members, including kinesin family members 5A, 5B, and 5C, and the kinesin light chain, are intricately linked to AD pathology. However, inconsistencies in the abundance of kinesin family members in AD patients underline the necessity for further exploration into the mechanistic impact of these motor proteins on neurodegeneration and axonal transport disruptions across a spectrum of neurological conditions. This review underscores the significance of kinesin-1's anterograde transport in AD. It emphasizes the need for investigations into the underlying mechanisms of the impact of motor protein across various neurological conditions. Despite current limitations in scientific literature, our study advocates for targeting kinesin and autophagy dysfunctions as promising avenues for novel therapeutic interventions and diagnostics in AD.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1432002"},"PeriodicalIF":4.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early life stress induces decreased expression of CB1R and FAAH and epigenetic changes in the medial prefrontal cortex of male rats. 早期生活压力会导致雄性大鼠内侧前额叶皮层中 CB1R 和 FAAH 的表达减少以及表观遗传学变化。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1474992
Arijana Demaili, Anna Portugalov, Mouna Maroun, Irit Akirav, Katharina Braun, Jörg Bock
{"title":"Early life stress induces decreased expression of CB1R and FAAH and epigenetic changes in the medial prefrontal cortex of male rats.","authors":"Arijana Demaili, Anna Portugalov, Mouna Maroun, Irit Akirav, Katharina Braun, Jörg Bock","doi":"10.3389/fncel.2024.1474992","DOIUrl":"10.3389/fncel.2024.1474992","url":null,"abstract":"<p><p>Several studies in both animal models and in humans have provided substantial evidence that early life stress (ELS) induces long-term changes in behavior and brain function, making it a significant risk factor in the aetiology of various mental disorders, including anxiety and depression. In this study, we tested the hypothesis that ELS in male rats (i) leads to increased anxiety and depressive-like symptoms; and (ii) that these behavioral changes are associated with functional alterations in the endocannabinoid system of the medial prefrontal cortex (mPFC). We further assessed whether the predicted changes in the gene expression of two key components of the endocannabinoid system, cannabinoid receptor 1 (CB1R) and the fatty acid amide hydrolase (FAAH), are regulated by epigenetic mechanisms. Behavioral profiling revealed that the proportion of behaviorally affected animals was increased in ELS exposed male rats compared to control animals, specifically showing symptoms of anhedonia and impaired social behavior. On the molecular level we observed a decrease in CB1R and FAAH mRNA expression in the mPFC of adult ELS exposed animals. These gene expression changes were accompanied by reduced global histone 3 acetylation in the mPFC, while no significant changes in DNA methylation and no significant changes of histone-acetylation at the promoter regions of the analyzed genes were detected. Taken together, our data provide evidence that ELS induces a long-term reduction of CB1R and FAAH expression in the mPFC of adult male rats, which may partially contribute to the ELS-induced changes in adult socio-emotional behavior.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1474992"},"PeriodicalIF":4.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased understanding of complex neuronal circuits in the cerebellar cortex. 加深对小脑皮层复杂神经元回路的了解。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1487362
Soyoung Jun, Heeyoun Park, Muwoong Kim, Seulgi Kang, Taehyeong Kim, Daun Kim, Yukio Yamamoto, Keiko Tanaka-Yamamoto
{"title":"Increased understanding of complex neuronal circuits in the cerebellar cortex.","authors":"Soyoung Jun, Heeyoun Park, Muwoong Kim, Seulgi Kang, Taehyeong Kim, Daun Kim, Yukio Yamamoto, Keiko Tanaka-Yamamoto","doi":"10.3389/fncel.2024.1487362","DOIUrl":"10.3389/fncel.2024.1487362","url":null,"abstract":"<p><p>The prevailing belief has been that the fundamental structures of cerebellar neuronal circuits, consisting of a few major neuron types, are simple and well understood. Given that the cerebellum has long been known to be crucial for motor behaviors, these simple yet organized circuit structures seemed beneficial for theoretical studies proposing neural mechanisms underlying cerebellar motor functions and learning. On the other hand, experimental studies using advanced techniques have revealed numerous structural properties that were not traditionally defined. These include subdivided neuronal types and their circuit structures, feedback pathways from output Purkinje cells, and the multidimensional organization of neuronal interactions. With the recent recognition of the cerebellar involvement in non-motor functions, it is possible that these newly identified structural properties, which are potentially capable of generating greater complexity than previously recognized, are associated with increased information capacity. This, in turn, could contribute to the wide range of cerebellar functions. However, it remains largely unknown how such structural properties contribute to cerebellar neural computations through the regulation of neuronal activity or synaptic transmissions. To promote further research into cerebellar circuit structures and their functional significance, we aim to summarize the newly identified structural properties of the cerebellar cortex and discuss future research directions concerning cerebellar circuit structures and their potential functions.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1487362"},"PeriodicalIF":4.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Somatostatin interneuron fate-mapping and structure in a Pten knockout model of epilepsy. Pten基因敲除癫痫模型中的体生长抑素中间神经元命运图谱和结构。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1474613
Austin W Drake, Lilian G Jerow, Justin V Ruksenas, Carlie McCoy, Steve C Danzer
{"title":"Somatostatin interneuron fate-mapping and structure in a Pten knockout model of epilepsy.","authors":"Austin W Drake, Lilian G Jerow, Justin V Ruksenas, Carlie McCoy, Steve C Danzer","doi":"10.3389/fncel.2024.1474613","DOIUrl":"10.3389/fncel.2024.1474613","url":null,"abstract":"<p><p>Disruption of inhibitory interneurons is common in the epileptic brain and is hypothesized to play a pivotal role in epileptogenesis. Abrupt disruption and loss of interneurons is well-characterized in status epilepticus models of epilepsy, however, status epilepticus is a relatively rare cause of epilepsy in humans. How interneuron disruption evolves in other forms of epilepsy is less clear. Here, we explored how somatostatin (SST) interneuron disruption evolves in quadruple transgenic Gli1-CreER<sup>T2</sup>, Pten<sup>fl/fl</sup>, SST-FlpO, and frt-eGFP mice. In these animals, epilepsy develops following deletion of the mammalian target of rapamycin (mTOR) negative regulator phosphatase and tensin homolog (Pten) from a subset of dentate granule cells, while downstream Pten-expressing SST neurons are fate-mapped with green fluorescent protein (GFP). The model captures the genetic complexity of human mTORopathies, in which mutations can be restricted to excitatory neuron lineages, implying that interneuron involvement is later developing and secondary. In dentate granule cell (DGC)-Pten knockouts (KOs), the density of fate-mapped SST neurons was reduced in the hippocampus, but their molecular phenotype was unchanged, with similar percentages of GFP+ cells immunoreactive for SST and parvalbumin (PV). Surviving SST neurons in the dentate gyrus had larger somas, and the density of GFP+ processes in the dentate molecular layer was unchanged despite SST cell loss and expansion of the molecular layer, implying compensatory sprouting of surviving cells. The density of Znt3-immunolabeled puncta, a marker of granule cell presynaptic terminals, apposed to GFP+ processes in the hilus was increased, suggesting enhanced granule cell input to SST neurons. Finally, the percentage of GFP+ cells that were FosB positive was significantly increased, implying that surviving SST neurons are more active. Together, findings suggest that somatostatin-expressing interneurons exhibit a combination of pathological (cell loss) and adaptive (growth) responses to hyperexcitability and seizures driven by upstream Pten KO excitatory granule cells.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1474613"},"PeriodicalIF":4.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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