Frontiers in Cellular Neuroscience最新文献

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A novel animal model of spontaneous epilepsy: Cdk5 knockout in pericyte-specific mice. 自发性癫痫的新型动物模型:在周细胞特异性小鼠中敲除 Cdk5。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1474231
Lin Lin, Xiaofei Hu, Weijun Hong, Tengwei Pan, Zhiren Wang, En Wang, Gang Wu
{"title":"A novel animal model of spontaneous epilepsy: Cdk5 knockout in pericyte-specific mice.","authors":"Lin Lin, Xiaofei Hu, Weijun Hong, Tengwei Pan, Zhiren Wang, En Wang, Gang Wu","doi":"10.3389/fncel.2024.1474231","DOIUrl":"10.3389/fncel.2024.1474231","url":null,"abstract":"<p><p>Changes in neurovascular unit components and their interactions play a crucial role in epileptogenesis and the pathological process of epilepsy. Currently, there is a lack of animal models that can accurately reflect the etiological impact of cerebrovascular lesions on epilepsy. In this study, we constructed cyclin-dependent kinase 5 conditional knockout mice in Cspg4 (pericyte marker)-positive cells using the Cre-LoxP system. The results revealed that this strain of mice exhibited significant seizure behaviors and epileptiform brain waves, loss of hippocampal and amygdala neurons, astrogliosis, decreased pericyte coverage, and reduced AQP4 polar distribution. Herein, we have developed a novel mouse model of spontaneous epilepsy, providing a critical animal model for studying the involvement of neurovascular unit factors in the development and progression of epilepsy.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arid3c identifies an uncharacterized subpopulation of V2 interneurons during embryonic spinal cord development. Arid3c 确定了胚胎脊髓发育过程中一个未定性的 V2 中间神经元亚群。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1466056
Estelle Renaux, Charlotte Baudouin, Olivier Schakman, Ondine Gay, Manon Martin, Damien Marchese, Younès Achouri, René Rezsohazy, Françoise Gofflot, Frédéric Clotman
{"title":"Arid3c identifies an uncharacterized subpopulation of V2 interneurons during embryonic spinal cord development.","authors":"Estelle Renaux, Charlotte Baudouin, Olivier Schakman, Ondine Gay, Manon Martin, Damien Marchese, Younès Achouri, René Rezsohazy, Françoise Gofflot, Frédéric Clotman","doi":"10.3389/fncel.2024.1466056","DOIUrl":"10.3389/fncel.2024.1466056","url":null,"abstract":"<p><p>Motor activity is organized by neuronal networks composed of motor neurons and a wide variety of pre-motor interneuron populations located in the brainstem and spinal cord. Differential expression and single-cell RNA sequencing studies recently unveiled that these populations subdivide into multiple subsets. However, some interneuron subsets have not been described yet, and the mechanisms contributing to this neuronal diversification have only been partly deciphered. In this study, we aimed to identify additional markers to further describe the diversity of spinal V2 interneuron populations. Here, we compared the transcriptome of V2 interneurons with that of the other cells of the embryonic spinal cord and extracted a list of genes enriched in V2 interneurons, including <i>Arid3c</i>. Arid3c identifies an uncharacterized subset of V2 that partially overlaps with V2c interneurons. These two populations are characterized by the production of Onecut factors and Sox2, suggesting that they could represent a single functional V2 unit. Furthermore, we show that the overexpression or inactivation of <i>Arid3c</i> does not alter V2 production, but its absence results in minor defects in locomotor execution, suggesting a possible function in subtle aspects of spinal locomotor circuit formation.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the underlying mechanisms of exercise as therapy for multiple sclerosis: insights from preclinical studies. 探索运动治疗多发性硬化症的内在机制:临床前研究的启示。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1460262
Yunpeng Du, Shuhan Dong, Wei Zou
{"title":"Exploring the underlying mechanisms of exercise as therapy for multiple sclerosis: insights from preclinical studies.","authors":"Yunpeng Du, Shuhan Dong, Wei Zou","doi":"10.3389/fncel.2024.1460262","DOIUrl":"10.3389/fncel.2024.1460262","url":null,"abstract":"<p><p>Multiple sclerosis (MS) is an immune-mediated disease of the central nervous system CNS characterized by demyelination, inflammation, and neurodegenerative changes, making it the most common nontraumatic disabling neurological disease in young adults. While current pharmacological treatments primarily target immunomodulation or immunosuppression, exercise is gaining increasing attention from the scientific community as an adjunctive therapy. This review explores the potential biological mechanisms of exercise in animal models of MS, focusing on its effects on neuroprotection and inflammation. The review examines how exercise inhibits pro-inflammatory microglial reactivity, stabilizes the blood-brain barrier, and enhances neurotrophic factor expression in animal studies. Future research directions are proposed by summarizing the evidence and limitations of existing animal models of MS, emphasizing the need to further validate these mechanisms in humans to better integrate exercise into the comprehensive management of MS. Additionally, investigating exercise-induced biomarkers for MS symptom reduction may provide a scientific basis for new therapeutic strategies.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of ferroptosis in neurodegenerative diseases. 铁蛋白沉积症在神经退行性疾病中的作用
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1475934
Yifan Fei, Yifei Ding
{"title":"The role of ferroptosis in neurodegenerative diseases.","authors":"Yifan Fei, Yifei Ding","doi":"10.3389/fncel.2024.1475934","DOIUrl":"https://doi.org/10.3389/fncel.2024.1475934","url":null,"abstract":"<p><p>Ferroptosis represents an iron<sup>-</sup> and lipid peroxidation (LPO)-mediated form of regulated cell death (RCD). Recent evidence strongly suggests the involvement of ferroptosis in various neurodegenerative diseases (NDs), particularly Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS), among others. The treatment of ferroptosis poses both opportunities and challenges in the context of ND. This review provides a comprehensive overview of characteristic features, induction and inhibition of ferroptosis, highlighting the ferroptosis inhibitor and the underlying mechanisms responsible for its occurrence. Moreover, the review explores how these mechanisms contribute to the pathogenesis and progression of major neurodegenerative disorders. Additionally, it presents novel insights into the role of ferroptosis in ND and summarizes recent advancements in the development of therapeutic approaches targeting ferroptosis. These insights and advancements hold potential to guide future strategies aimed at effectively managing these debilitating medical conditions.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Manipulation of DHPS activity affects dendritic morphology and expression of synaptic proteins in primary rat cortical neurons. 操纵 DHPS 活性会影响原代大鼠皮质神经元的树突形态和突触蛋白的表达。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1465011
Paola Cavalli, Anna Raffauf, Sergio Passarella, Martin Helmuth, Daniela C Dieterich, Peter Landgraf
{"title":"Manipulation of DHPS activity affects dendritic morphology and expression of synaptic proteins in primary rat cortical neurons.","authors":"Paola Cavalli, Anna Raffauf, Sergio Passarella, Martin Helmuth, Daniela C Dieterich, Peter Landgraf","doi":"10.3389/fncel.2024.1465011","DOIUrl":"10.3389/fncel.2024.1465011","url":null,"abstract":"<p><p>Deoxyhypusine synthase (DHPS) catalyzes the initial step of hypusine incorporation into the eukaryotic initiation factor 5A (eIF5A), leading to its activation. The activated eIF5A, in turn, plays a key role in regulating the protein translation of selected mRNAs and therefore appears to be a suitable target for therapeutic intervention strategies. In the present study, we analyzed the role of DHPS-mediated hypusination in regulating neuronal homeostasis using lentivirus-based gain and loss of function experiments in primary cortical cultures from rats. This model allows us to examine the impact of DHPS function on the composition of the dendritic and synaptic compartments, which may contribute to a better understanding of cognitive function and neurodevelopment <i>in vivo</i>. Our findings revealed that shRNA-mediated DHPS knockdown diminishes the amount of hypusinated eIF5A (eIF5A<sup>Hyp</sup>), resulting in notable alterations in neuronal dendritic architecture. Furthermore, in neurons, the synaptic composition was also affected, showing both pre- and post-synaptic changes, while the overexpression of DHPS had only a minor impact. Therefore, we hypothesize that interfering with the eIF5A hypusination caused by reduced DHPS activity impairs neuronal and synaptic homeostasis.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel loss-of-function KCNB1 gene variant in a twin with global developmental delay and seizures. 在一对患有全面发育迟缓和癫痫发作的双胞胎中发现了一种新型功能缺失的 KCNB1 基因变异。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1477989
Rían W Manville, Claire L Illeck, Cesar Santos, Richard Sidlow, Geoffrey W Abbott
{"title":"A novel loss-of-function <i>KCNB1</i> gene variant in a twin with global developmental delay and seizures.","authors":"Rían W Manville, Claire L Illeck, Cesar Santos, Richard Sidlow, Geoffrey W Abbott","doi":"10.3389/fncel.2024.1477989","DOIUrl":"10.3389/fncel.2024.1477989","url":null,"abstract":"<p><p>Human voltage-gated potassium (Kv) channels are expressed by a 40-member gene family that is essential for normal electrical activity and is closely linked to various excitability disorders. Function-altering sequence variants in the <i>KCNB1</i> gene, which encodes the neuronally expressed Kv2.1 channel, are associated with neurodevelopmental disorders including developmental delay with or without epileptic activity. In this study, we describe a 40-month-old fraternal twin who presented with severe neurodevelopmental delay. Electroencephalogram recordings at 19 months of age revealed poor sleep architecture and the presence of multifocal epileptiform discharges. The individual's fraternal twin was neurotypical, and there was no family history of neurodevelopmental delay or seizures. Whole genome sequencing at 33 months of age for the proband revealed a <i>de novo</i> variant in <i>KCNB1</i> [c.1154C > T/p.Pro385Leu], encoding a proline-to-leucine substitution at residue 385, in the extracellular region immediately preceding Kv2.1 transmembrane segment 6 (S6). Cellular electrophysiological analysis of the effects of the gene variant in heterologously expressed Kv2.1 demonstrated that homozygous Kv2.1-P385L channels were completely non-functional. Channels generated by a 50/50 expression of wild-type Kv2.1 and Kv2.1-P385L, designed to mimic the proband's heterozygous status, revealed a partially dominant-negative effect, resulting in an 81% reduction in current magnitude. The dramatic loss of function in Kv2.1 is the most likely cause of the severe developmental delay and seizure activity in the proband, further enriching our phenotypic understanding of <i>KCNB1</i> developmental encephalopathies.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative, real-time imaging of spreading depolarization-associated neuronal ROS production. 扩增去极化相关神经元 ROS 生成的定量实时成像。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-11 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1465531
Marc André Ackermann, Susanne Monika Buchholz, Katharina Dietrich, Michael Müller
{"title":"Quantitative, real-time imaging of spreading depolarization-associated neuronal ROS production.","authors":"Marc André Ackermann, Susanne Monika Buchholz, Katharina Dietrich, Michael Müller","doi":"10.3389/fncel.2024.1465531","DOIUrl":"https://doi.org/10.3389/fncel.2024.1465531","url":null,"abstract":"<p><p>Spreading depolarization (SD) causes a massive neuronal/glial depolarization, disturbs ionic homeostasis and deranges neuronal network function. The metabolic burden imposed by SD may also generate marked amounts of reactive oxygen species (ROS). Yet, proper optical tools are required to study this aspect with spatiotemporal detail. Therefore, we earlier generated transgenic redox indicator mice. They express in excitatory projection neurons the cytosolic redox-sensor roGFP, a reduction/oxidation sensitive green fluorescent protein which is ratiometric by excitation and responds reversibly to redox alterations. Using adult male roGFPc mice, we analyzed SD-related ROS production in CA1 <i>stratum pyramidale</i> of submerged slices. SD was induced by K<sup>+</sup> microinjection, O<sub>2</sub> withdrawal or mitochondrial uncoupling (FCCP). The extracellular DC potential deflection was accompanied by a spreading wavefront of roGFP oxidation, confirming marked neuronal ROS generation. Hypoxia-induced SD was preceded by a moderate oxidation, which became intensified as the DC potential deflection occurred. Upon K<sup>+</sup>-induced SD, roGFP oxidation slowly recovered within 10-15 min in some slices. Upon FCCP-or hypoxia-induced SD, recovery was limited. Withdrawing extracellular Ca<sup>2+</sup> markedly dampened the SD-related roGFP oxidation and improved its reversibility, confirming a key-role of neuronal Ca<sup>2+</sup> load in SD-related ROS generation. Neither mitochondrial uncoupling, nor inhibition of NADPH oxidase or xanthine oxidase abolished the SD-related roGFP oxidation. Therefore, ROS generation during SD involves mitochondria as well as non-mitochondrial sources. This first-time analysis of SD-related ROS dynamics became possible based on quantitative redox imaging in roGFP mice, an advanced approach, which will contribute to further decipher the molecular understanding of SD in brain pathophysiology.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ALS-like pathology diminishes swelling of spinal astrocytes in the SOD1 animal model. 在 SOD1 动物模型中,类似 ALS 的病理变化会减轻脊髓星形胶质细胞的肿胀。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-10 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1472374
Tereza Filipi, Jana Tureckova, Ondrej Vanatko, Martina Chmelova, Monika Kubiskova, Natalia Sirotova, Stanislava Matejkova, Lydia Vargova, Miroslava Anderova
{"title":"ALS-like pathology diminishes swelling of spinal astrocytes in the SOD1 animal model.","authors":"Tereza Filipi, Jana Tureckova, Ondrej Vanatko, Martina Chmelova, Monika Kubiskova, Natalia Sirotova, Stanislava Matejkova, Lydia Vargova, Miroslava Anderova","doi":"10.3389/fncel.2024.1472374","DOIUrl":"https://doi.org/10.3389/fncel.2024.1472374","url":null,"abstract":"<p><p>Astrocytes are crucial for the functioning of the nervous system as they maintain the ion homeostasis via volume regulation. Pathological states, such as amyotrophic lateral sclerosis (ALS), affect astrocytes and might even cause a loss of such functions. In this study, we examined astrocytic swelling/volume recovery in both the brain and spinal cord of the SOD1 animal model to determine the level of their impairment caused by the ALS-like pathology. Astrocyte volume changes were measured in acute brain or spinal cord slices during and after exposure to hyperkalemia. We then compared the results with alterations of extracellular space (ECS) diffusion parameters, morphological changes, expression of the Kir4.1 channel and the potassium concentration measured in the cerebrospinal fluid, to further disclose the link between potassium and astrocytes in the ALS-like pathology. Morphological analysis revealed astrogliosis in both the motor cortex and the ventral horns of the SOD1 spinal cord. The activated morphology of SOD1 spinal astrocytes was associated with the results from volume measurements, which showed decreased swelling of these cells during hyperkalemia. Furthermore, we observed lower shrinkage of ECS in the SOD1 spinal ventral horns. Immunohistochemical analysis then confirmed decreased expression of the Kir4.1 channel in the SOD1 spinal cord, which corresponded with the diminished volume regulation. Despite astrogliosis, cortical astrocytes in SOD1 mice did not show alterations in swelling nor changes in Kir4.1 expression, and we did not identify significant changes in ECS parameters. Moreover, the potassium level in the cerebrospinal fluid did not deviate from the physiological concentration. The results we obtained thus suggest that ALS-like pathology causes impaired potassium uptake associated with Kir4.1 downregulation in the spinal astrocytes, but based on our data from the cortex, the functional impairment seems to be independent of the morphological state.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142498013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alzheimer's disease induced neurons bearing PSEN1 mutations exhibit reduced excitability. 携带 PSEN1 突变的阿尔茨海默病诱导神经元表现出兴奋性降低。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-09 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1406970
Simon Maksour, Rocio K Finol-Urdaneta, Amy J Hulme, Mauricio E Castro Cabral-da-Silva, Helena Targa Dias Anastacio, Rachelle Balez, Tracey Berg, Calista Turner, Sonia Sanz Muñoz, Martin Engel, Predrag Kalajdzic, Leszek Lisowski, Kuldip Sidhu, Perminder S Sachdev, Mirella Dottori, Lezanne Ooi
{"title":"Alzheimer's disease induced neurons bearing <i>PSEN1</i> mutations exhibit reduced excitability.","authors":"Simon Maksour, Rocio K Finol-Urdaneta, Amy J Hulme, Mauricio E Castro Cabral-da-Silva, Helena Targa Dias Anastacio, Rachelle Balez, Tracey Berg, Calista Turner, Sonia Sanz Muñoz, Martin Engel, Predrag Kalajdzic, Leszek Lisowski, Kuldip Sidhu, Perminder S Sachdev, Mirella Dottori, Lezanne Ooi","doi":"10.3389/fncel.2024.1406970","DOIUrl":"https://doi.org/10.3389/fncel.2024.1406970","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a devastating neurodegenerative condition that affects memory and cognition, characterized by neuronal loss and currently lacking a cure. Mutations in <i>PSEN1</i> (Presenilin 1) are among the most common causes of early-onset familial AD (fAD). While changes in neuronal excitability are believed to be early indicators of AD progression, the link between <i>PSEN1</i> mutations and neuronal excitability remains to be fully elucidated. This study examined iPSC-derived neurons (iNs) from fAD patients with <i>PSEN1</i> mutations S290C or A246E, alongside CRISPR-corrected isogenic cell lines, to investigate early changes in excitability. Electrophysiological profiling revealed reduced excitability in both <i>PSEN1</i> mutant iNs compared to their isogenic controls. Neurons bearing S290C and A246E mutations exhibited divergent passive membrane properties compared to isogenic controls, suggesting distinct effects of <i>PSEN1</i> mutations on neuronal excitability. Additionally, both <i>PSEN1</i> backgrounds exhibited higher current density of voltage-gated potassium (Kv) channels relative to their isogenic iNs, while displaying comparable voltage-gated sodium (Nav) channel current density. This suggests that the Nav/Kv imbalance contributes to impaired neuronal firing in fAD iNs. Deciphering these early cellular and molecular changes in AD is crucial for understanding disease pathogenesis.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142498014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deprivation of visual input alters specific subset of inhibitory neurons and affect thalamic afferent terminals in V1 of rd1 mouse. 剥夺视觉输入会改变rd1小鼠V1中特定抑制神经元亚群并影响丘脑传入终端
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2024-10-09 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1422613
Kashish Parnami, Anushka Surana, Vineet Choudhary, Anwesha Bhattacharyya
{"title":"Deprivation of visual input alters specific subset of inhibitory neurons and affect thalamic afferent terminals in V1 of <i>rd1</i> mouse.","authors":"Kashish Parnami, Anushka Surana, Vineet Choudhary, Anwesha Bhattacharyya","doi":"10.3389/fncel.2024.1422613","DOIUrl":"https://doi.org/10.3389/fncel.2024.1422613","url":null,"abstract":"<p><p>Retinitis Pigmentosa (RP) is a heterogenous group of inherited disorder, and its progression not only affects the retina but also the primary visual cortex. This manifests imbalances in the excitatory and inhibitory neurotransmission. Here, we investigated if changes in cortical functioning is linked to alterations in GABAergic population of neurons and its two important subsets, somatostatin (SST) and parvalbumin (PV) neuron in <i>rd1</i> model of retinal degeneration (RD). We demonstrate marked decrease in the proportion of SST neurons in different layers of cortex whereas PV neurons were less affected. Moreover, we found reduced expression of glutamatergic thalamic afferents (VGLUT2) due to lack of visual activity. These results suggest PV neurons are likely recruited by the cortical circuitry to increase the inhibitory drive and compensate the disrupted inhibition-excitation balance. However, reduced SST expression perhaps results in weakening of stimulus selectivity. Delineating their functional role during RD will provide insights for acquisition of high-resolution vision thereby improving current state of vision restoration.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142498015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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