Frontiers in Cellular Neuroscience最新文献

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Long-term live-cell imaging of GFAP+ astroglia and laminin+ vessels in organotypic mouse brain slices using microcontact printing. 利用微接触打印技术对小鼠有机脑切片中的 GFAP+星形胶质细胞和层粘连蛋白+血管进行长期活细胞成像。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-27 eCollection Date: 2025-01-01 DOI: 10.3389/fncel.2025.1540150
Christian Humpel
{"title":"Long-term live-cell imaging of GFAP+ astroglia and laminin+ vessels in organotypic mouse brain slices using microcontact printing.","authors":"Christian Humpel","doi":"10.3389/fncel.2025.1540150","DOIUrl":"10.3389/fncel.2025.1540150","url":null,"abstract":"<p><p>Organotypic brain slices are three-dimensional, 150-μm-thick sections derived from postnatal day 10 mice that can be cultured for several weeks <i>in vitro</i>. However, these slices pose challenges for live-cell imaging due to their thickness, particularly without access to expensive two-photon microscopy. In this study, we present an innovative method to label and visualize specific brain cell populations in living slices. Using microcontact printing, antibodies are applied directly onto the slices in a controlled 400-μm-diameter pattern. Astrocytes are labeled with glial fibrillary acidic protein (GFAP), and vessels are labeled with laminin. Subsequently, slices are incubated with secondary fluorescent antibodies (green fluorescent Alexa-488 or red fluorescent Alexa-546) and visualized using an inverted fluorescence microscope. This approach offers a cost-effective and detailed visualization technique for astroglia and vessels in living brain slices, enabling investigation to be conducted over several weeks.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"19 ","pages":"1540150"},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pharmacological approaches in drug-resistant pediatric epilepsies caused by pathogenic variants in potassium channel genes. 钾通道基因致病性变异引起的耐药儿童癫痫的药理学方法。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-24 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1512365
Ilaria Filareto, Ilaria Mosca, Elena Freri, Francesca Ragona, Laura Canafoglia, Roberta Solazzi, Barbara Castellotti, Giuliana Messina, Cinzia Gellera, Maria Virginia Soldovieri, Paolo Ambrosino, Maurizio Taglialatela, Jacopo C DiFrancesco, Tiziana Granata
{"title":"Pharmacological approaches in drug-resistant pediatric epilepsies caused by pathogenic variants in potassium channel genes.","authors":"Ilaria Filareto, Ilaria Mosca, Elena Freri, Francesca Ragona, Laura Canafoglia, Roberta Solazzi, Barbara Castellotti, Giuliana Messina, Cinzia Gellera, Maria Virginia Soldovieri, Paolo Ambrosino, Maurizio Taglialatela, Jacopo C DiFrancesco, Tiziana Granata","doi":"10.3389/fncel.2024.1512365","DOIUrl":"10.3389/fncel.2024.1512365","url":null,"abstract":"<p><p>Variants in genes encoding for voltage-gated K<sup>+</sup> (Kv) channels are frequent cause of drug-resistant pediatric epilepsies. Obtaining a molecular diagnosis gives the opportunity to assess the efficacy of pharmacological strategies based on <i>in vitro</i> features of mutant channels. In this retrospective observational study, we selected patients with drug-resistant pediatric epilepsies caused by variants in potassium channel encoding genes, followed at the Fondazione IRCCS Istituto Neurologico Carlo Besta of Milan, Italy. After the experimental characterization of variants' functional properties in transiently transfected Chinese Hamster Ovary (CHO) cells, we identified drugs to be used as pharmacological approaches. We recruited six patients carrying different missense variants in four Kv channels (Kv7.2, Kv7.3, Kv3.1, and K<sub>Na</sub>1.1). <i>In vitro</i> experiments demonstrated that variants in Kv7 channels induced loss-of-function (LoF) effects, while those affecting Kv3.1 or K<sub>Na</sub>1.1 led to gain-of-function (GoF). Moreover, we found that the Kv7 channels activator gabapentin was able to revert the LoF effects caused by Kv7.2/Kv7.3 variants, and the potassium channel-blocker fluoxetine counteracted the GoF effects in Kv3.1 or K<sub>Na</sub>1.1 variants. According to experimental data, patients carrying Kv7 variants were treated with gabapentin. While this treatment resulted successful in two patients (#1, Kv7.2 G310S variant; #3, Kv7.3 V359L + Kv7.3 D542N), it resulted detrimental in the remaining case (#2, Kv7.2 D535E), requiring drug withdrawal. The application <i>in vivo</i> of fluoxetine to counteract GoF effects induced by Kv3.1 or K<sub>Na</sub>1.1 variants determined a significant reduction of both seizure frequency and behavior disturbances in patient #4 (Kv3.1 V425M), and in both subjects carrying K<sub>Na</sub>1.1 variants (#5, S937G and #6, R262Q). However, for the latter case, this drug was halted due to severe behavioral side effects. For most of the patients herein reported, pharmacological strategies, selected according to the <i>in vitro</i> functional properties of Kv-channels pathogenic variants, resulted in a significant improvement of both epileptic and cognitive features.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1512365"},"PeriodicalIF":4.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional characterization of endocytic signals in the SynDIG/PRRT family members SynDIG1 and SynDIG4 in heterologous cells and neurons. SynDIG/PRRT家族成员SynDIG1和SynDIG4在异源细胞和神经元内吞信号的功能表征
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-23 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1526034
David J Speca, Chun-Wei He, Christina M Meyer, Erin C Scott, Elva Díaz
{"title":"Functional characterization of endocytic signals in the SynDIG/PRRT family members SynDIG1 and SynDIG4 in heterologous cells and neurons.","authors":"David J Speca, Chun-Wei He, Christina M Meyer, Erin C Scott, Elva Díaz","doi":"10.3389/fncel.2024.1526034","DOIUrl":"10.3389/fncel.2024.1526034","url":null,"abstract":"<p><p>The transmembrane protein Synapse Differentiation Induced Gene 4 (SynDIG4), also known as Proline-rich transmembrane protein 1 (PRRT1), is an AMPA-type glutamate receptor (AMPAR) auxiliary factor that is necessary for maintaining extra-synaptic pools of GluA1. Loss of SynDIG4, and the subsequent decrease in extra-synaptic GluA1, has been found to significantly impact synaptic plasticity in the hippocampus. However, how SynDIG4 establishes and maintains these pools is unclear. Previous studies suggested that endocytic machinery is important for maintaining a pool of mobile surface AMPARs, and that proteins associated with such cellular machinery are critical for proper protein trafficking and internalization. Given that SynDIG4 co-localizes with GluA1 in early and recycling endosomes in cultured hippocampal neurons, we sought to identify the sorting signals that target SynDIG4 to endosomes to further elucidate the role of SynDIG4 in GluA1 trafficking. In this study, we report that SynDIG4 possesses a YxxΦ sorting motif, 178-YVPV-181, responsible for binding to the AP-2 complex cargo-sorting subunit μ2. This motif appears critical for proper SynDIG4 internalization, as SynDIG4 mutant 178-AVPA-181, which disrupts binding to μ2, induces aberrant SynDIG4 accumulation at the plasma-membrane of heterologous cells and primary rat hippocampal neurons. We also show that SynDIG4 mutants lacking an endocytic signal co-localize with GluA1 but less so with GluA2 on the surface of heterologous cells. Furthermore, we show that another family member, SynDIG1, is enriched in the trans-Golgi network (TGN) and can traffic between the TGN and plasma membrane. We have identified a non-canonical μ2 binding sequence in SynDIG1 that induces aberrant accumulation at the plasma membrane of heterologous cells and primary rat hippocampal neurons, suggesting a conserved role for μ2-mediated endocytosis within the SynDIG family. These results provide important insight into the mechanisms by which SynDIG proteins are targeted to endosomal compartments as a step in understanding SynDIG-mediated regulation of AMPAR trafficking.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1526034"},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11798926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interneuronal modulations as a functional switch for cortical computations: mechanisms and implication for disease. 作为皮质计算功能开关的神经元间调节:机制及其对疾病的影响。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-23 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1479579
Yann Zerlaut, Alexandra Tzilivaki
{"title":"Interneuronal modulations as a functional switch for cortical computations: mechanisms and implication for disease.","authors":"Yann Zerlaut, Alexandra Tzilivaki","doi":"10.3389/fncel.2024.1479579","DOIUrl":"10.3389/fncel.2024.1479579","url":null,"abstract":"<p><p>Understanding cortical inhibition and its diverse roles remains a key challenge in neurophysiological research. Traditionally, inhibition has been recognized for controlling the stability and rhythmicity of network dynamics, or refining the spatiotemporal properties of cortical representations. In this perspective, we propose that specific types of interneurons may play a complementary role, by modulating the computational properties of neural networks. We review experimental and theoretical evidence, mainly from rodent sensory cortices, that supports this view. Additionally, we explore how dysfunctions in these interneurons may disrupt the network's ability to switch between computational modes, impacting the flexibility of cortical processing and potentially contributing to various neurodevelopmental and psychiatric disorders.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1479579"},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression. 由解剖定位和CART表达定义的黑色素浓缩激素神经元亚群的电生理特性。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-22 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1439752
Rafiat Damilola Adekunle, Mohammed Sohel Chowdhury, Lisa Z Fang, Michiru Hirasawa
{"title":"Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression.","authors":"Rafiat Damilola Adekunle, Mohammed Sohel Chowdhury, Lisa Z Fang, Michiru Hirasawa","doi":"10.3389/fncel.2024.1439752","DOIUrl":"10.3389/fncel.2024.1439752","url":null,"abstract":"<p><strong>Introduction: </strong>Melanin-concentrating hormone (MCH) neurons are essential regulators of energy and glucose homeostasis, sleep-wake behaviors, motivation, learning and memory. These neurons are anatomically distributed across the medial (MH) and lateral hypothalamus (LH), and the adjacent zona incerta (ZI), which may represent functional subgroups with distinct connectivity with different brain regions. Furthermore, MCH neurons can be classified according to co-expression of neuropeptides, such as cocaine and amphetamine- regulated transcript (CART).</p><p><strong>Methods: </strong>To identify functional similarities and differences of MCH subpopulations, we characterized their intrinsic electrophysiological properties using whole cell current clamp recording on acute brain slices from male and female mice.</p><p><strong>Results: </strong>MCH neurons were classified into subgroups according to their anatomical localization in three MCH-rich brain areas: MH, LH and ZI. Among the three brain regions, ZI MCH neurons were the least excitable while LH MCH neurons were the most excitable. Furthermore, grouping MCH neurons according to CART co-expression revealed that MCH/CART- cells are uniquely depolarized and excitable, and display H-currents. These MCH/CART- cells were mainly found in the LH, which may in part explain why LH MCH neurons are more excitable. While some sex differences were found, the majority of parameters investigated were not different.</p><p><strong>Discussion: </strong>Our results suggest that MCH/CART- cells are electrophysiologically distinct, whereas MCH/CART+ cells are largely similar despite their diffuse distribution in the hypothalamus. It is therefore a combination of intrinsic electrophysiological properties and neurochemical identities, in addition to anatomy and connectivity that are likely to be critical in defining functional subpopulations of MCH neurons.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1439752"},"PeriodicalIF":4.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the impact of the stargazin V143L mutation on the dynamics of the AMPA receptor: stargazin complex. 探讨星参蛋白V143L突变对AMPA受体:星参蛋白复合物动力学的影响。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-17 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1505846
Raquel P Gouveia, Carlos A V Barreto, Rita Melo, Ana Luísa Carvalho, Irina S Moreira
{"title":"Exploring the impact of the stargazin V143L mutation on the dynamics of the AMPA receptor: stargazin complex.","authors":"Raquel P Gouveia, Carlos A V Barreto, Rita Melo, Ana Luísa Carvalho, Irina S Moreira","doi":"10.3389/fncel.2024.1505846","DOIUrl":"10.3389/fncel.2024.1505846","url":null,"abstract":"<p><p>Stargazin, a transmembrane AMPAR regulatory protein (TARP), plays a crucial role in facilitating the transport of AMPA receptors to the cell surface, stabilising their localisation at synapses and influencing their gating properties. The primary objective of this study was to investigate the effect of the V143L mutation in stargazin, previously linked to intellectual disability, on the interaction between stargazin and AMPA receptors. To achieve this, we conducted a thorough examination of eight distinct molecular dynamics simulations of AMPA receptor-stargazin complexes, each associated with different conductance levels. Through extensive analysis of complex interface structures and dynamics, we revealed that the stargazin V143L mutation had a more pronounced destabilising effect on complexes with lower conductance levels than on the conductive states of the receptor, suggesting a potential association with impaired synaptic transmission in individuals with this mutation.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1505846"},"PeriodicalIF":4.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bibliometric insights into astrocytic roles in depression and treatment. 星形细胞在抑郁症和治疗中的作用的文献计量学见解。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-15 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1521398
Linsun Lin, Ziyi Guo, Zhuoyu Ren, Yanchen Feng, Peigang Fang, Tao Wang, Min Chen
{"title":"Bibliometric insights into astrocytic roles in depression and treatment.","authors":"Linsun Lin, Ziyi Guo, Zhuoyu Ren, Yanchen Feng, Peigang Fang, Tao Wang, Min Chen","doi":"10.3389/fncel.2024.1521398","DOIUrl":"https://doi.org/10.3389/fncel.2024.1521398","url":null,"abstract":"<p><strong>Objective: </strong>Depression is a mental disorder that significantly impairs both physical and mental health. Recent studies have shown that reactive astrogliosis have gained significant attention for their involvement in the pathophysiology of depression. However, there is no bibliometric analysis in this research field. This study aims to provide a comprehensive overview of the knowledge structure and research hotspots regarding the role of astrocytes in the mechanisms and treatment of depression through bibliometric analysis. The scope of the literature review encompasses both basic and clinical research.</p><p><strong>Methods: </strong>Publications related to astrocytes in depression and treatment from 2014 to 2023 were searched in the Web of Science Core Collection (WoSCC) database. VOSviewer, CiteSpace, and the R package \"bibliometrix\" were used to conduct this bibliometric analysis.</p><p><strong>Results: </strong>From 2014 to 2023, a total of 1,502 documents from 78 countries on astrocytes in depression and treatment were analyzed from 169 journals, with the most co-cited journals being the Journal of Neuroscience and PNAS. China Medical University was the most productive institution. The analysis identified key authors like Verkhratsky Alexei and Baoman Li, and major co-cited references by Rajkowska and Liddelow. Keywords such as \"synaptic plasticity,\" \"astrocytes,\" and \"neuroinflammation\" revealed research trends focusing on molecular mechanisms, gut microbiota, and inflammation.</p><p><strong>Conclusion: </strong>This is the first bibliometric study to comprehensively summarize the research trends and advancements regarding astrocytes in depression and its treatment. Through this bibliometric analysis, we aim to enhance the understanding of the significance of astrocytes in depression research and provide new perspectives and insights for future investigations. We hope that this study will facilitate a broader integration of basic and clinical research, offering novel approaches for the treatment of depression.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1521398"},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developmental regression of novel space preference in an autism spectrum disorder model is unlinked to GABAergic and social circuitry. 自闭症谱系障碍模型中新空间偏好的发育回归与gaba能和社会回路无关。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-15 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1513347
Hirofumi Asano, Masaya Arai, Aito Narita, Takayuki Kuroiwa, Mamoru Fukuchi, Yuhei Yoshimoto, Soichi Oya, Goichi Miyoshi
{"title":"Developmental regression of novel space preference in an autism spectrum disorder model is unlinked to GABAergic and social circuitry.","authors":"Hirofumi Asano, Masaya Arai, Aito Narita, Takayuki Kuroiwa, Mamoru Fukuchi, Yuhei Yoshimoto, Soichi Oya, Goichi Miyoshi","doi":"10.3389/fncel.2024.1513347","DOIUrl":"https://doi.org/10.3389/fncel.2024.1513347","url":null,"abstract":"<p><p>Autism spectrum disorder (ASD) is characterized by social deficits and restricted behaviors, with developmental defects in GABAergic circuits proposed as a key underlying etiology. Here, we introduce the V-Y assay, a novel space preference test in which one arm of the Y-maze is initially hidden and later revealed as a novel space. Using an ASD mouse model with <i>FOXG1</i> haploinsufficiency, which exhibits ASD-like social impairments that can be either exacerbated or ameliorated by GABAergic circuit manipulations, we observed impaired novel space preference and exploratory behavior in the V-Y assay. Interestingly, unlike social phenotypes, novel space preference was initially established by 3 weeks of age but regressed by 6 weeks. Furthermore, alterations in GABAergic signaling via <i>Gad2</i> mutation did not affect novel space preference, in contrast to their impact on social behaviors. These findings reveal that the regression of novel space preference in ASD follows a distinct developmental trajectory from GABA-driven social impairments, providing new insights into the mechanisms underlying ASD.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1513347"},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral encephalitis and seizures cause rapid depletion of neuronal progenitor cells and alter neurogenesis in the adult mouse dentate gyrus. 病毒性脑炎和癫痫发作引起神经元祖细胞的快速耗竭,并改变成年小鼠齿状回的神经发生。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-14 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1528918
Alberto Pauletti, Polina Gurlo, Edna Weiß, Ana Beatriz DePaula-Silva, Karen S Wilcox, Sonja Bröer
{"title":"Viral encephalitis and seizures cause rapid depletion of neuronal progenitor cells and alter neurogenesis in the adult mouse dentate gyrus.","authors":"Alberto Pauletti, Polina Gurlo, Edna Weiß, Ana Beatriz DePaula-Silva, Karen S Wilcox, Sonja Bröer","doi":"10.3389/fncel.2024.1528918","DOIUrl":"10.3389/fncel.2024.1528918","url":null,"abstract":"<p><p>Infections impacting the central nervous system (CNS) constitute a substantial predisposing factor for the emergence of epileptic seizures. Given that epilepsy conventionally correlates with hippocampal sclerosis and neuronal degeneration, a potentially innovative avenue for therapeutic intervention involves fostering adult neurogenesis, a process primarily occurring within the subgranular zone of the dentate gyrus (DG) through the differentiation of neural stem cells (NSC). While experimental seizures induced by chemoconvulsants or electrical stimulation transiently enhance neurogenesis, the effects of encephalitis and the resultant virus-induced seizures remain inadequately understood. Thus, this study employed the Theiler's Murine Encephalomyelitis Virus (TMEV) model of virus-induced seizures in adult C57BL/6J mice to investigate the impact of infection-induced seizures on neurogenesis at three distinct time points [3, 7, and 14 days post-infection (dpi)]. Immunohistochemical analysis revealed a reduction in the overall number of proliferating cells post-infection. More notably, the specific cell types exhibiting proliferation diverged between TMEV and control (CTR) mice: (1) Neuronal progenitors (doublecortin, DCX<sup>+</sup>) were almost entirely absent at 3 dpi in the dorsal DG. They resumed proliferation at 14 dpi, but, did not recover to CTR levels, and displayed aberrant migration patterns. (2) The number of proliferating NSCs significantly decreased within the dorsal DG of TMEV mice at 14 dpi compared to CTR, while (3) a heightened population of proliferating astrocytes was observed. Most observed changes were not different between seizing and non-seizing infected mice. In summary, our findings demonstrate that viral infection rapidly depletes neuronal progenitor cells and causes aberrant migration of the remaining ones, potentially contributing to hyperexcitability. Additionally, the increased differentiation toward glial cell fates in infected mice emerges as a possible additional pro-epileptogenic mechanism.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1528918"},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11772278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The lactate metabolism and protein lactylation in epilepsy. 癫痫的乳酸代谢和蛋白乳酸化。
IF 4.2 3区 医学
Frontiers in Cellular Neuroscience Pub Date : 2025-01-14 eCollection Date: 2024-01-01 DOI: 10.3389/fncel.2024.1464169
Xi Kuang, Shuang Chen, Qingmei Ye
{"title":"The lactate metabolism and protein lactylation in epilepsy.","authors":"Xi Kuang, Shuang Chen, Qingmei Ye","doi":"10.3389/fncel.2024.1464169","DOIUrl":"10.3389/fncel.2024.1464169","url":null,"abstract":"<p><p>Protein lactylation is a new form of post-translational modification that has recently been proposed. Lactoyl groups, derived mainly from the glycolytic product lactate, have been linked to protein lactylation in brain tissue, which has been shown to correlate with increased neuronal excitability. Ischemic stroke may promote neuronal glycolysis, leading to lactate accumulation in brain tissue. This accumulation of lactate accumulation may heighten neuronal excitability by upregulating protein lactylation levels, potentially triggering post-stroke epilepsy. Although current clinical treatments for seizures have advanced significantly, approximately 30% of patients with epilepsy remain unresponsive to medication, and the prevalence of epilepsy continues to rise. This study explores the mechanisms of epilepsy-associated neuronal death mediated by lactate metabolism and protein lactylation. This study also examines the potential for histone deacetylase inhibitors to alleviate seizures by modifying lactylation levels, thereby offering fresh perspectives for future research into the pathogenesis and clinical treatment of epilepsy.</p>","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"18 ","pages":"1464169"},"PeriodicalIF":4.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11772370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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