I. Plotnikova, G. Magomedov, I. Zharkova, Elena Miroshnichenko, Viktor Plotnikov
{"title":"Jelly formulated with different carbohydrate profiles: Quality evaluation","authors":"I. Plotnikova, G. Magomedov, I. Zharkova, Elena Miroshnichenko, Viktor Plotnikov","doi":"10.21603/2308-4057-2022-2-535","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-535","url":null,"abstract":"Jelly is a popular confectionery product but it has a high content of easily digestible sugars, namely 70–85%. Therefore, modern confectioners are trying to develop new formulations of jelly with reduced sweetness and sugar content. One of the ways to achieve that is to use starch syrup instead of white sugar. Another benefit of starch syrup is that it can slow down the drying and staling of jelly. \u0000We studied three types of starch syrup (low-conversion, confectionery, high-conversion), glucose-fructose syrup, and sugar-free jelly samples based on them. Jelly based on sugar and confectionery syrup was used as the control sample. The main quality indicators were analyzed against standard values; the sensory parameters were determined by the descriptor-profile analysis; and water activity was measured by using a HygroPalm Rotronic hygrometer. The microbiological safety of the experimental jelly samples was assessed after 12 weeks of their storage in plastic containers. \u0000The sample based on confectionery syrup had the most optimal profile, with moderate sweetness and taste richness, good jelly-like texture, viscoelasticity, plasticity, a color similar to that of the control, and no effect of wetting or stickiness. The samples based on starch syrup had a 1.4–2.4-fold decrease in easily digestible sugars and a 1.9–3.4-fold increase in polysaccharides, compared to the control. During storage, the samples based on high-conversion starch syrup and glucose-fructose syrup were less likely to dry out than the others, with their water activity decreasing to a greater extent. The microbiological analysis after storage showed the absence of pathogenic microorganisms and coliform bacteria in three out of the four jelly samples. \u0000Using various types of starch syrup and glucose-fructose syrup instead of white sugar allows for a greater range of jelly types with different carbohydrate profiles and a longer shelf life.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42610150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Semenov, A. Slavyanskiy, D. Mitroshina, N. Lebedeva
{"title":"Thermodynamic factor and vacuum crystallization","authors":"E. Semenov, A. Slavyanskiy, D. Mitroshina, N. Lebedeva","doi":"10.21603/2308-4057-2022-2-542","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-542","url":null,"abstract":"Sucrose crystallization depends on various thermal phenomena, which makes them an important scientific issue for the sugar industry. However, the rationale and theory of sucrose crystallization still remain understudied. Among the least described problems is the effect of time and temperature on the condensation rate of sucrose molecules on crystallization nuclei in a supersaturated sugar solution. This article introduces a physical and mathematical heat transfer model for this process, as well as its numerical analysis. \u0000The research featured a supersaturated sugar solution during sucrose crystallization and focused on the condensation of sucrose molecules on crystallization nuclei. The study involved the method of physical and mathematical modeling of molecular mass transfer, which was subjected to a numerical analysis. \u0000While crystallizing in a vacuum boiling pan, a metastable solution went through an exothermal reaction. In a supersaturated solution, this reaction triggered a transient crystallization of solid phase molecules and a thermal release from the crystallization nuclei into the liquid phase. This exogenous heat reached 39.24 kJ/kg and affected the mass transfer kinetics. As a result, the temperature rose sharply from 80 to 86 °C. \u0000The research revealed the effect of temperature and time on the condensation of solids dissolved during crystalline sugar production. The model involved the endogenous heat factor. The numerical experiment proved that the model reflected the actual process of sucrose crystallization. The obtained correlations can solve a number of problems that the modern sugar industry faces.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43488478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparative study of phytochemical, antioxidant, anticarcinogenic, and antidiabetic potential of coriander (Coriandrum sativum L.): Microgreen and mature plant","authors":"G. M. Dhakshayani, S. Priya","doi":"10.21603/2308-4057-2022-2-539","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-539","url":null,"abstract":"Microgreens are immature edible leafy greens with a higher concentration of phytonutrients than in mature leaves, which makes them a novel functional food. This research featured antioxidant, anticarcinogenic, and antidiabetic properties of coriander microgreens. \u0000Aqueous and ethanolic extractions of coriander microgreens and mature leaves underwent a phytochemical analysis of antioxidant potential using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method and the ferric reducing antioxidant power (FRAP) assay. The analysis of antidiabetic and anticarcinogenic properties included the method of α-amylase enzyme inhibition and the MTT colorimetric assay. \u0000The screening test inferred the presence of alkaloids, terpenoids, glycosides, steroids, tannins, flavonoids, phenols, carbohydrates, and proteins in both microgreens and mature leaves. The quantitative analysis showed that the ethanolic extract of the microgreen sample exhibited higher total phenols. Total flavonoids, steroids, carbohydrates, and proteins were higher both in microgreen extracts, if compared with those of mature leaves. Ascorbic acid, chlorophyll-a, chlorophyll-b, and carotenoids demonstrated a more substantial presence in mature leaves. The gas chromatography-mass spectrometry (GC/MS) analysis of coriander microgreens revealed such bioactive compounds as thienopyrimidines, phenolic amide, imidazo pyridazine, phenolic constituents, and essential oil. Mature leaves were rich in phenolic compounds, steroids, terpenoids, essential oils, and fatty acid esters. All these substances are known for their therapeutic antioxidant, antidiabetic, and anticarcinogenic properties. The microgreen samples exhibited greater ferric reducing antioxidant power, α-amylase enzyme inhibition, and cytotoxicity activity at a lower concentration of extract than mature leaves. \u0000Coriander microgreens proved to have a promising antioxidant, anticarcinogenic, and antidiabetic potential and can be used in daily food additives.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46447256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Voroshilin, M. Kurbanova, E. Ostapova, Eduard Makhambetov, A. Petrov, Mohammed El Amine Khelef
{"title":"Effect of gelatin drying methods on its amphiphilicity","authors":"R. Voroshilin, M. Kurbanova, E. Ostapova, Eduard Makhambetov, A. Petrov, Mohammed El Amine Khelef","doi":"10.21603/2308-4057-2022-2-534","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-534","url":null,"abstract":"Gelatin is a natural amphiphilic biopolymer that is widely used in food products, pharmaceuticals, and cosmetics. We studied the effect of spray and freeze drying on the solubility and amphiphilicity of gelatin samples. \u0000The control sample was a commercially produced edible gelatin. The experimental samples were spray- and freeze-dried gelatins obtained by enzymatic-acid hydrolysis of cattle bone. Amino acid sequences were determined by matrix-activated laser desorption/ionization. Solubility was assessed visually. Bloom strength of the gelatin gels was measured by a texture analyzer. The ProtScale online service was used to predict the amphiphilic topology of gelatin proteins. Molecular weight distribution of proteins was carried out by electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate. \u0000Spray drying reduced protein degradation and retained more α-chains, while freeze drying increased gelatin’s hydrophobicity and decreased its solubility. The predicted topology of protein hydrophobicity based on the amino acid sequences was in line with our results on solubility. The freeze-dried gelatin had a 18% larger amount of low-molecular weight peptides, compared to the control and the spray-dried samples. This was probably caused by the cleavage of peptides during the drying process. Thus, freeze drying can lead to maximum degradation of gelatin components, which may be associated with a longer heat treatment, compared to spray drying. \u0000Thus, spray drying is more suitable for gelatin, since this method improves the stability of its outer and inner structure, ensuring high hydrophilic properties.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44282764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Near-infrared spectroscopy as a green technology to monitor coffee roasting","authors":"Krzysztof Wójcicki","doi":"10.21603/2308-4057-2022-2-536","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-536","url":null,"abstract":"Wet chemistry methods are traditionally used to evaluate the quality of a coffee beverage and its chemical characteristics. These old methods need to be replaced with more rapid, objective, and simple analytical methods for routine analysis. Near-infrared spectroscopy is an increasingly popular technique for nondestructive quality evaluation called a green technology. \u0000Our study aimed to apply near-infrared spectroscopy to evaluate the quality of coffee samples of different origin (Brazil, Guatemala, Peru, and Kongo). Particularly, we analyzed the roasting time and its effect on the quality of coffee. The colorimetric method determined a relation between the coffee color and the time of roasting. Partial least squares regression analysis assessed a possibility of predicting the roasting conditions from the near-infrared spectra. \u0000The regression results confirmed the possibility of applying near-infrared spectra to estimate the roasting conditions. The correlation between the spectra and the roasting time had R2 values of 0.96 and 0.95 for calibration and validation, respectively. The root mean square errors of prediction were low – 0.92 and 1.05 for calibration and validation, respectively. We also found a linear relation between the spectra and the roasting power. The quality of the models differed depending on the coffee origin and sub-region. All the coffee samples showed a good correlation between the spectra and the brightness (L* parameter), with R2 values of 0.96 and 0.95 for the calibration and validation curves, respectively. \u0000According to the results, near-infrared spectroscopy can be used together with the chemometric analysis as a green technology to assess the quality of coffee.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47164496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Giro, L. Ilina, A. Kulikovsky, I. Ziruk, A. Giro
{"title":"Molecular genetic studies of microbiocenosis and microstructure of jejunum wall in young rams grown on biofortified feed additives","authors":"T. Giro, L. Ilina, A. Kulikovsky, I. Ziruk, A. Giro","doi":"10.21603/2308-4057-2022-2-541","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-541","url":null,"abstract":"The research featured the effect of a diet fortified with essential microelements on the ruminal microbiota of young rams. Ruminal microbiota is largely responsible for feed digestibility and body functioning of cattle. \u0000The study involved the contents of the rumens and jejuna of seven-month-old rams of the Edilbaev breed, which were subjected to a biofortified diet. The diet included the Russian feed additives Yoddar-Zn and DAFS-25 represent a protein-carbohydrate complex with plant silicon. The microflora of the digestive tract was tested using the molecular genetic method of terminal restriction fragment length polymorphism (T-RFLP) sequestration. The microstructural studies of the jejunum samples exploited light microscopy. \u0000The feed additives increased the population of cellulolytic and lactate-fermenting bacteria, as well as the Prevotella sp. microbiome and bifidobacteria in the rumen samples. The data obtained revealed the effect of essential microelements on the taxonomic pattern of microorganisms and the microflora profile. The research revealed the ratio of normal, opportunistic, pathogenic, nonculturable, and transit microflora. The jejunum wall samples obtained from the experimental group that fed on Yoddar-Zn and DAFS-25 had a more distinct micropicture of mucous membrane. Their rumen microflora balance had fewer pathogenic and opportunistic microorganisms, which was also confirmed by the jejunum morphology. \u0000The feed additives DAFS-25 and Yoddar-Zn proved beneficial for ram diet and inhibited the negative effect of pathogenic treponemas on the rumen. The additives improved digestion, absorption, and assimilation of food nutrients, as well as increased the livestock yield.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45093411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Yogurt-like product from lupine (Lupinus albus L.) milk as an alternative to dairy products","authors":"Nazan Kavas","doi":"10.21603/2308-4057-2022-2-546","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-546","url":null,"abstract":"Recently, the number of people suffering from allergy to caw’s milk has increased. Lupine, a plant rich in protein, can be a good alternative product for non-dairy products production. We aimed to obtain a yogurt-like product based on lupine milk and evaluate its properties. \u0000Lupine milk was obtained from lupine seeds, egg white protein powder, disaccharides, and starter cultures were added to the milk to obtain yogurt-like products: samples with maltose, samples with lactose, and samples without sugars. Physico-chemical and microbiological characteristics of the products were determined by the standard techniques. Sensory attributes were evaluated by trained panelists. \u0000In the study, the effects of egg white protein powder and disaccharides on the activities of starter cultures and the properties of the yogurt-like products obtained were investigated. The relationship between the addition of sugar and the growth of starter cultures was found to be significant (P < 0.05). In terms of physico-chemical, rheological, and microbiological properties, the yogurt-like products obtained from lupine milk with disaccharides demonstrated good results, especially the sample with maltose. Sensory analysis revealed high sensory properties of the yogurt-like products. \u0000Yogurt-like products from lupine milk can be used as an alternative to cow’s milk fermented products, but more detailed studies should be conducted on their formulations.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49606164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Faheid, Ibrahim R.S. Rizk, Y. Kishk, G. Ragab, S. Mostafa
{"title":"Carboxymethyl cellulose and psyllium husk in gluten-free pasta","authors":"S. Faheid, Ibrahim R.S. Rizk, Y. Kishk, G. Ragab, S. Mostafa","doi":"10.21603/2308-4057-2022-2-540","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-540","url":null,"abstract":"Formulating high-quality pasta from wheat-free materials is a technological challenge. We aimed to make gluten-free pasta with carboxymethyl cellulose and psyllium husk and evaluate their effect on the quality of the final product. \u0000Gluten-free pasta was produced from rice flour, white corn flour, potato starch, soy protein isolate, and carboxymethyl cellulose or psyllium husk used as binding agents. Then, we evaluated the effect of these hydrocolloids on the color, texture, cooking quality, and sensory characteristics of the product. \u0000The uncooked gluten-free pasta containing psyllium husk showed significantly higher values of hardness compared to the samples with carboxymethyl cellulose, while the cooked pasta with psyllium husk had a significantly lower nitrogen loss. Also, psyllium husk improved the texture of the cooked gluten-free pasta, providing the highest values of resilience, springiness, and chewiness. Generally, the psyllium husk samples received higher quality values for texture, cooking quality, and sensory parameters, compared to the pasta with carboxymethyl cellulose. \u0000Psyllium husk showed a better ability to bind gluten-free pasta than carboxymethyl cellulose. Consequently, psyllium husk could become a feasible alternative to wheat gluten in producing high-quality gluten-free pasta.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48244062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Digital inventory of agricultural land plots in the Kemerovo Region","authors":"A. Rada, A. Kuznetsov","doi":"10.21603/2308-4057-2022-2-529","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-529","url":null,"abstract":"Cadastral and geodetic land works are expensive, which makes aerial photography extremely valuable for land traceability and inventory. The present research objective was to develop a new digital survey technology for registration of agricultural lands. We assessed the accuracy of the new method and evaluated its decision support options. The study featured the case of the Kemerovo Region (Kuzbass), Russia. \u0000The aerial survey took place in 2021 and involved 17 municipalities of the Kemerovo Region. The software and hardware complex included an unmanned aerial vehicle (UAV) and a module for aerial photography. Photogrammetric, cartometric, and satellite methods were used to define the coordinates of feature points. We developed new software (Sovhoz.avi) to perform the land inventory. \u0000The photogrammetric and cartographic methods proved efficient in determining the feature points and boundaries of land plots. They also appeared accurate enough for land inventory and decision support. The study updated the available land inventory data. About 30% of all land plots were recorded incorrectly; some plots marked as agricultural appeared to belong to the local forest reserves or urban territories. Incorrect data (1.64%) were excluded from the official inventory. The survey covered a total area of 41 000 ha and revealed 1700 illegally used land plots. The updated inventory of unused lands included 3825 new plots (163 400 ha), which can attract prospective investors. \u0000The results can be used by the local authorities to make land management decisions and identify illegal land use.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41906754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Gadouche, K. Zerrouki, Azdinia Zidane, A. Ababou, Ibtissem Bachir Elazaar, Dounya Merabet, Wissam Henniche, S. Ikhlef
{"title":"Genoprotective, antimutagenic, and antioxidant effect of methanolic leaf extract of Rhamnus alaternus L. from the Bissa mountains in Algeria","authors":"L. Gadouche, K. Zerrouki, Azdinia Zidane, A. Ababou, Ibtissem Bachir Elazaar, Dounya Merabet, Wissam Henniche, S. Ikhlef","doi":"10.21603/2308-4057-2022-2-530","DOIUrl":"https://doi.org/10.21603/2308-4057-2022-2-530","url":null,"abstract":"Rhamnus alaternus L. is a Rhamnaceae shrub and a popular traditional medicine in Algeria. The present research objective was to investigate the antioxidant, genotoxic, and antigenotoxic properties of R. alaternus methanolic leaf extract. \u0000Antiradical scavenging activity was tested by α, α-diphenyl-β-picrylhydrazyl free radical scavenging and β-carotene bleaching method. DNA damage and repair were measured by the Allium cepa test with sodium azide as a mutagenic agent. Mitotic index and chromosomal aberrations were calculated by microscopy of meristem roots stained with 2% carmine acetic. \u0000The methanolic extract of R. alaternus leaves inhibited the free radical DPPH (IC50 = 0.74 ± 0.3 mg/mL) and prevented the oxidation of β-carotene (50.71 ± 4.17%). The root phenotyping showed that sodium azide changed their color and shape, decreased their stiffness, and significantly reduced their length. The roots treated with both R. alaternus leaf extract and sodium azide demonstrated a better root growth. The roots treated with the methanolic extract were much longer than the control roots (P < 0.001). The microscopy images of root meristem treated with the sodium azide mitodepressant agent showed significant chromosomal aberrations, which indicated a disruption of the cell cycle. \u0000The R. alaternus leaf extract appeared to have a beneficial effect on cytotoxicity. The antioxidant properties of R. alaternus L. makes this plant an excellent genoportector.","PeriodicalId":12426,"journal":{"name":"Foods and Raw Materials","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68373490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}