Folia histochemica et cytobiologica最新文献

{"title":"Morphology and immunohistochemical characteristics of the otic ganglion in the chinchilla (Chinchilla laniger Molina).","authors":"Waldemar Sienkiewicz,&nbsp;Jacek Kuchinka,&nbsp;Agnieszka Dudek,&nbsp;Elżbieta Nowak,&nbsp;Jerzy Kaleczyc,&nbsp;Małgorzata Radzimirska,&nbsp;Aleksander Szczurkowski","doi":"10.5603/FHC.a2023.0001","DOIUrl":"https://doi.org/10.5603/FHC.a2023.0001","url":null,"abstract":"<p><strong>Introduction: </strong>The available literature provides relatively little information on the morphology of the autonomic head ganglia in rodents including their neurochemical codding.</p><p><strong>Material and methods: </strong>Morphological investigations of the otic ganglion of the chinchilla were performed using the modified acetylcholinesterase method. The cellular structure was investigated with histological techniques and neurochemical properties were studied with the double-labelling immunofluorescence method.</p><p><strong>Results: </strong>Macromorphological investigations allowed the otic ganglion to be identified as a compact, oval agglomeration of neurons and nerve fibers. Multidimensional cross-sections revealed densely arranged neuronal perikarya and two populations of nerve cells differing in size were distinguished. The large cells (40-50 μm) accounted for about 80% of the neurons in the cross-sections. Moreover, a small number of intraganglionic nerve fibers was observed. Immunohistochemical staining revealed that over 85% of the neuronal cell bodies in the otic ganglion contained immunoreactivity to VAChT or ChAT. VIP-immunoreactive perikarya comprised approximately 10% of the ganglionic cells. Double staining revealed the presence of VAChT+ and NOS+ neurons which amounted to about 45% of the nerve cells in the otic ganglion. NOS+ only perikarya comprised approx. 15% of all the neurons. Immunoreactivity to enkephalins, substance P, somatostatin, and galanin was expressed in single nerve cell bodies and nerve fibers except numerous substance P+ intraganglionic nerve fibers. Some of them were stained also for CGRP. Single neurons stained for tyroxine hydroxylase.</p><p><strong>Conclusions: </strong>Our results, compared with findings in other rodent species suggest the existence of interspecies differences in the morphology, cellular structure, and immunohistochemical properties of the head autonomic ganglia in mammals.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"61 1","pages":"17-25"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9207694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effect of exosomes derived from hepatocyte-growth-factor-overexpressing adipose mesenchymal stem cells on liver injury.","authors":"Liushenyan Yu, Junchao Xue, Yanyan Wu, Hanyu Zhou","doi":"10.5603/fhc.95291","DOIUrl":"10.5603/fhc.95291","url":null,"abstract":"<p><p>Adipose mesenchymal stem cell-derived exosomes (ADMSC-Exo) are a new strategy for the treatment of liver injury. However, mesenchymal stem cells (MSCs) exert therapeutic effects mainly by secreting hepatocyte growth factor (HGF). Therefore, we investigated the role of exosomes derived from ADMSC that overexpress HGF (ADMSCHGF-Exo) on liver injury.</p><p><strong>Material and methods: </strong>ADMSCs were isolated from young BALB/c female mice. Then exosomes derived from ADMSC transfecting negative control (ADMSCNC-Exo) and HGF overexpression (ADMSCHGF-Exo) were isolated and identified by quantitative polymerase chain reaction (qPCR), flow cytometry, western blot, transmission electron microscope and Nanosight particle tracking analysis. These exosomes were injected into male mice via tail vein after inducing liver injury by administering 40% carbon tetrachloride (CCl₄)-olive oil twice a week (3 mL/kg, subcutaneously) for 6 weeks. Liver injury and liver collagen fiber accumulation were determined by histopathological analysis. Then, the levels of serum liver function indexes (alanine aminotransferase, aspartate aminotransferase, albumin, total bilirubin), hepatocyte-specific markers (albumin, cytokeratin-18 and hepatocyte nuclear factor 4α), hepatic fibrosis-related proteins (α-smooth muscle actin and collagen I) and Rho GTPase (cell division cycle 42 and ras-related C3 botulinum toxin substrate 1) were determined by Enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, Western blot and qPCR.</p><p><strong>Results: </strong>ADMSCs were identified by high expression of CD105 and CD44 molecules and low expression of CD45 and CD34. ADMSCs-Exo, ADMSCNC-Exo and ADMSCHGF-Exo transfected cells had similar expression of exosome-specific membrane proteins (CD63, CD81 and CD9). Mice with CCl₄-induced liver injury exhibited abnormal serum liver function indexes, altered expression of hepatocyte-specific markers, hepatic fibrosis-related proteins and Rho GTPase protein as well as histopathological changes and collagen fiber accumulation in the liver. These changes were reversed by ADMSC-Exo, ADMSCNC-Exo and ADMSCHGF-Exo administration with ADMSCHGF-Exo displaying the most significant impact.</p><p><strong>Conclusions: </strong>ADMSCHGF-Exo exerted a hepatoprotective effect in mice with experimental liver injury by alleviating hepatic fibrosis and restoring liver function.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":"160-171"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41107814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of autophagy promotes sonodynamic therapy-induced apoptosis of pancreatic cancer cells.","authors":"Xiaoxue Du, Jiaming Song, Ziwen Zhang, Jia Liu, Dan Xu","doi":"10.5603/fhc.95262","DOIUrl":"10.5603/fhc.95262","url":null,"abstract":"<p><strong>Introduction: </strong>Sonodynamic therapy (SDT), a promising non-invasive therapeutic modality, has attracted increasing attention in the treatment of pancreatic cancer (PC). At present, the role of autophagy in SDT of PC remains unclear. This study aims to explore the role of autophagy in SDT of PC and its effect on apoptosis of PC cells.</p><p><strong>Material and methods: </strong>PC cells (Capan-1 and BxPC-3) underwent incubation with 5-aminolevulinic acid (5-ALA) or/and ultrasound (US) exposure (control, 5-ALA, US, and SDT groups), followed by measurement of cell apoptosis and autophagy. Specifically, cell viability, apoptosis, and the expression of apoptosis-related proteins (cleaved Caspase-3, Bax, and Bcl-2) were measured using CCK-8 assay, flow cytometry, and western blot analysis, respectively. The mitochondrial morphology was observed with the transmission electron microscopy, accompanied by the detection of autophagosome marker (LC3) co-located with Mito and the protein expression of LC3II/I. Before SDT treatment, the autophagy inhibitor 3-MA and the apoptosis inhibitor z-VAD were respectively added to PC cell cultures to evaluate the effects of autophagy inhibition on apoptosis and apoptosis inhibition on autophagy in PC cells.</p><p><strong>Results: </strong>Compared with the control group, cell viability was inhibited and cell apoptosis and autophagy were enhanced in the SDT group, while cell viability, autophagy, and apoptosis in the 5-ALA and US groups were not significantly changed. Moreover, 3-MA treatment inhibited autophagy and accelerated apoptosis, whereas z-VAD treatment reduced apoptosis but did not affect autophagy in PC cells.</p><p><strong>Conclusions: </strong>Autophagy was activated in SDT-treated PC cells, and inhibition of autophagy promoted cell apoptosis in PC cells.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":"172-182"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41130064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin alleviates H2O2-induced senescence and apoptosis in rat nucleus pulposus-derived mesenchymal stem cells.","authors":"Tian Xie,&nbsp;Ruijie Pan,&nbsp;Wenzhuo Huang,&nbsp;Sheng Dong,&nbsp;Shizhen Wu,&nbsp;Yuhui Ye","doi":"10.5603/FHC.a2023.0007","DOIUrl":"https://doi.org/10.5603/FHC.a2023.0007","url":null,"abstract":"<p><strong>Introduction: </strong>Transplantation of mesenchymal stem cells (MSCs) has been reported to be a novel promising target for the regeneration of degenerated intervertebral discs (IVDs). However, the culture and survival limitations of MSCs remain challenging for MSC-based biological therapy. Myricetin, a common natural flavonoid, has been suggested to possess antiaging and antioxidant abilities. Therefore, we investigated the biological function of myricetin, and its related mechanisms involving cell senescence in intervertebral disc degeneration (IDD).</p><p><strong>Material and methods: </strong>The nucleus pulposus-derived mesenchymal stem cells (NPMSCs) were isolated from 4-month-old Sprague-Dawley (SD) rats and identified by examining surface markers and multipotent differentiation. Rat NPMSCs were cultured in an MSC culture medium or culture medium with different concentrations of H2O2. Myricetin or the combination of myricetin and EX527 were added to the culture medium to investigate the effects of myricetin. Cell viability was evaluated by cell counting kit-8 assays (CCK-8). The apoptosis rate was determined using Annexin V/PI dual staining. The mitochondrial membrane potential (MMP) was analyzed by a fluorescence microscope after JC-1 staining. The cell senescence was determined by SA-β-Gal staining. MitoSOX green was used to selectively estimate mitochondrial reactive oxygen species (ROS) Apoptosis-associated proteins (Bax, Bcl2, and cleaved caspase-3), senescence markers (p16, p21, and p53), and SIRT1/PGC-1α signaling pathway-related proteins (SIRT1 and PGC-1α) were evaluated by western blotting.</p><p><strong>Results: </strong>The cells isolated from nucleus pulposus (NP) tissues met the criteria for MSCs. Myricetin showed no cytotoxicity up to a concentration of 100 μM in rat NPMSCs cultured for 24 h. Myricetin pretreatment exhibited protective effects against H₂O₂-induced apoptosis. Myricetin could also alleviate H₂O₂-induced mitochondrial dysfunctions of increased mitochondrial ROS production and reduced MMP. Moreover, myricetin pretreatment delayed rat NPMSC senescence, as evidenced by decreased exppression of senescence indicators. Pretreatment of NPMSCs with 10 μM EX527, a selective inhibitor of SIRT1, prior to exposure to 100 μM H2O2, reversed the inhibitory effects of myricetin on cell apoptosis.</p><p><strong>Conclusions: </strong>Myricetin could affect the SIRT1/PGC-1α pathway to protect mitochondrial functions and alleviate cell senescence in H₂O₂-treated NPMSCs.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"61 2","pages":"98-108"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10173171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effect of autophagy induced by rapamycin versus intermittent fasting in animal model of fatty liver.","authors":"Sara Adel Hosny, Mohammed Hafez Ahmed Moustafa, Fatma Mahmoud Mehina, Marwa Mohamed Sabry","doi":"10.5603/fhc.95905","DOIUrl":"10.5603/fhc.95905","url":null,"abstract":"<p><strong>Introduction: </strong>High-fructose, high-fat diet consumption (HFHF) is one of the primary causes of non-alcoholic fatt liver disease (NAFLD), which is due to impaired beta-oxidation or apolipoprotein secretion by hepatocytes. Activation of autophagy in hepatocytes could be a therapeutic method against hepatic complications. This study was designed to compare effects of rapamycin and intermittent fasting-inducing autophagy in rats with experimentally induced nonalcoholic fatty liver.</p><p><strong>Material and methods: </strong>Male rats were divided into five groups: C (control, n = 6), the experimental group (EX) subdivided, EXIa (HFHF, n = 6), EXIb (recovery, n = 6), EXII (rapamycin, n = 6) and EXIII (intermittent fasting, n = 6). All rats in the experimental group received HFHF diet for 8 weeks to induce nonalcoholic-fatty liver and obesity. Then, for the next 8 weeks the animals received either a daily oral dose of rapamycin (EXII group) or to intermittent fasting (IF) for 16 hours daily (EXIII group). Blood samples were drawn, and serum TG concentration as well as ALT and AST activities were determined. Hepatic sections were examined by light and electron microscopy. LC3B immunohistochemical staining, morphometric and statistical studies were performed.</p><p><strong>Results: </strong>Subgroups EXIa (HFHF subgroup) and EXIb (Recovery subgroup) showed marked increase in TG, ALT, and AST levels associated with loss of normal hepatic architecture, cytoplasmic vacuolations and faint LC3B immunoreactivity. Ultrathin sections exhibited many autophagosomes in hepatocytes. On the other hand, rapamycin (EXII) and IF (EXIII) groups showed significant improvement to a variable extent in comparison to EXI groups.</p><p><strong>Conclusions: </strong>It could be concluded that rapamycin and intermittent fasting significantly improved NAFLD-induced changes of liver structure and function by inducing autophagy in hepatocytes.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":"205-216"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical analysis of the thymus in newborn and adult yaks (Bos grunniens).","authors":"Z. Qian, C. Yan, Y. Sijiu, H. Junfeng, P. Yangyang, Xu Gengquan, Yang Kun","doi":"10.5603/FHC.a2022.0017","DOIUrl":"https://doi.org/10.5603/FHC.a2022.0017","url":null,"abstract":"INTRODUCTION\u0000The thymus is the site of development and maturation of functional T lymphocytes and is critically important to the immune system. The purpose of this study was to examine the expression of markers of T lymphocytes, macrophages, dendritic cells, B lymphocytes and plasmocytes in the yak thymus.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Twenty healthy male yaks were divided into newborn (2-4 weeks old, n = 10) and adult (3-4 years old, n = 10) group. qRT-PCR was used to evaluate the mRNA expression level of the main markers of the studied cell types. Immunohistochemistry was used to detect the distribution of CD3+ T lymphocytes, CD68+ macrophages, SIRPα+ dendritic cells, CD79α+ B lymphocytes, IgA and IgG+ plasmocytes.\u0000\u0000\u0000RESULTS\u0000Within the same age group, the mRNA expression of CD3ε was highest (P < 0.05), followed by that of CD68, SIRPα, CD79α, IgG and IgA. Furthermore, CD3ε, CD68, and SIRPα mRNA expression levels were higher in newborn yaks than in the adult ones (P < 0.05), whereas those of CD79α, IgA, and IgG were higher in adults (P < 0.05). Immunohistochemical results showed localization of CD3+ T lymphocytes in the thymic cortex and medulla. CD68+ macrophages, SIRPα+ dendritic cells, CD79α+ B lymphocytes, IgA+ and IgG+ plasmocytes were mainly observed in the cortico-medullary region and medulla. In the same age group, the frequency of CD3+ T lymphocytes was higher than that of CD68+ macrophages and SIRPα+ dendritic cells (P < 0.05), followed by those of CD79α+ B lymphocytes and IgA+ and IgG+ plasmocytes. No significant difference was observed between B lymphocyte and plasmocyte frequencies in the yak thymus in both age groups (P > 0.05). The frequency of CD3+, CD68+ and SIRPα+ cells decreased from newborns to adults (P < 0.05). However, the frequencies of CD79α+, IgA+ and IgG+ cells increased from newborn to adult yaks (P < 0.05).\u0000\u0000\u0000CONCLUSIONS\u0000The thymus of newborn yaks is well-developed, with higher numbers of T lymphocytes, macrophages, and dendritic cells than those in the adult thymus. However, higher frequencies of plasmocytes and B lymphocytes were detected in the adult thymus, suggesting that adults may better resist infections through humoralimmunity as this organ undergoes involution. Furthermore, there was no significant difference in the number of IgA and IgG plasmocytes, which differs from what is observed in rodents and humans. This difference might be related to the fact that yaks live in low-oxygen plateaus.","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42369076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of neuropeptide Y in the brain of the male native Thai chicken.","authors":"B. Kamkrathok, Y. Chaiseha","doi":"10.5603/FHC.a2022.0016","DOIUrl":"https://doi.org/10.5603/FHC.a2022.0016","url":null,"abstract":"INTRODUCTION\u0000. Neuropeptide Y (NPY), a 36 amino acid neurotransmitter/neuromodulator, is involved in food intake and parental cares in birds. NPY is associated with the regulation of the reproductive system in the female native Thai chickens. However, the role of NPY in the male native Thai chicken has not been studied. Therefore, the objective of this study was to investigate the distributions of NPY immunoreactive (-ir) neurons and fibers in the brain of the male native Thai chickens.\u0000\u0000\u0000MATERIAL AND METHODS\u0000. The distribution of NPY-ir neurons and fibers in the hen brain was elucidated utilizing immunohistochemical technique.\u0000\u0000\u0000RESULTS\u0000. The distributions of NPY-ir neurons and fibers were located throughout the brain, predominantly in the hypothalamus. The numbers of NPY-ir neurons within the nucleus paraventricularis magnocellularis (PVN) were significantly higher than those of the nucleus septalis lateralis (SL), nucleus supraopticus (SOv), and nucleus inferioris hypothalami and nucleus infundibuli hypothalami (IH-IN). In addition, the numbers of NPY-ir neurons within the SL, SOv, and IH-IN were significantly higher than those of the tractus septomesencephalicus and nucleus dorsolateralis anterior thalami.\u0000\u0000\u0000CONCLUSIONS\u0000. These results indicated, for the first time, that the distributions of NPY-ir neurons and fibers in the brain of the male native Thai chickens were markedly observed in the hypothalamus, especially within the PVN, implicating that the NPYergic system within the PVN might be related to the regulation of feeding behavior and parental cares in this equatorial species.","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44616894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of adipose mesenchymal stem cell-derived exosomes-loaded β-chitin nanofiber hydrogel for wound healing.","authors":"Ying Liu, Yunen Liu, Yan Zhao, Minna Wu, Shun Mao, P. Cong, Rufei Zou, Mingxiao Hou, Hongxu Jin, Yongli Bao","doi":"10.5603/fhc.a2022.0015","DOIUrl":"https://doi.org/10.5603/fhc.a2022.0015","url":null,"abstract":"INTRODUCTION\u0000Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment.\u0000\u0000\u0000MATERIALS AND METHODS\u0000The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) and the wound healing ability of AMSC-exos-loaded β-chitin nanofiber (β-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively.\u0000\u0000\u0000RESULTS\u000080 and 160 μg/mL AMSC-exos could promote the proliferation and migration of mouse fibroblasts cells. Furthermore, AMSC-exos-loaded β-ChNF hydrogel resulted in a significant acceleration rate of wound closure, notably acceleration of re-epithelialization, and increased collagen expression based on the rat full-thickness skin injury model. The transcriptomics and proteomics studies revealed the changes of the expression of 18 genes, 516 transcripts and 250 proteins. The metabolic pathways, tight junction, NF-κB signaling pathways were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway. Complement factor D (CFD) and downstream Aldolase A (Aldoa) and Actn2 proteins in rats treated with AMSC-exos-loaded β-ChNF hydrogel were noticed and further confirmed by ELISA and Western blot.\u0000\u0000\u0000CONCLUSION\u0000These findings suggested that AMSC-exos-loaded β-ChNF hydrogel could promote wound healing with the mechanism which is related to the effect of AMSC-exos on CFD and downstream proteins.","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42072865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chronic moderate methionine administration induced hyperhomocysteinemia associated with cardiovascular disease phenotype in the sand rat Psammomys obesus.","authors":"Fouzia Zerrouk, B. Chaouad, A. Ghoul, Naima Chalour, A. Moulahoum, Zineb Khiari, M. Cherifi, Souhila Aouichat, K. Houali, Y. Benazzoug","doi":"10.5603/FHC.a2022.0013","DOIUrl":"https://doi.org/10.5603/FHC.a2022.0013","url":null,"abstract":"INTRODUCTION\u0000Cardiovascular diseases were defined as coronary artery, cerebrovascular, or peripheral arterial disease.Hyperhomocysteinemia (Hhcy) is an independent risk factor of cardiovascular diseases, including atherosclerosis. Our previous studies demonstrated the involvement of Hhcy in cardiovascular remodeling in the sand rat Psammomys obesus.\u0000\u0000\u0000MATERIAL AND METHODS\u0000An experimental Hhcy was induced, in the sand rat Psammomys obesus, by a daily intraperitoneal injection of 70 mg/kg of methionine for a total duration of 6 months. The impact of Hhcy on the cellular and matrix structures of the heart, aorta and liver was analyzed using histological techniques. Additionally we treated primary cultures of aortic smooth muscle cells (SMCs) with high concentration of methionine to investigate the effects of methionine at the cellular level.\u0000\u0000\u0000RESULTS\u0000A moderate Hhcy induced a significant increase in the extracellular matrix components particularly collagens which accumulated in the interstitial and perivascular spaces in the studied organs indicating a developing fibrosis. A liver steatosis was also observed following methionine treatment. Further analysis of the aorta showed that Hhcy also induced vascular alterations including SMCs reorientation and proliferation associated with aneurysm formation.\u0000\u0000\u0000CONCLUSIONS\u0000Our results show for the first time that Hhcy can induce a cardiovascular and liver diseases phenotype in Psammomys obesus, a species previously shown to be a good model for the studies of diabetes and other metabolism-related pathologies.","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45828799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GLI1 is involved in HIF-1α-induced migration, invasion, and epithelial-mesenchymal transition in glioma cells.","authors":"Yihai Lin, Liang Guo","doi":"10.5603/FHC.a2022.0014","DOIUrl":"https://doi.org/10.5603/FHC.a2022.0014","url":null,"abstract":"INTRODUCTION\u0000Glioma is characterized by hypoxia that activates the hypoxia inducible factor (HIF) pathway and controls a myriad of genes that drive cancer progression. HIF-1α promotes GLI1 transferring to the nucleus by activating the hedgehog pathway under hypoxic conditions. However, their mechanisms in glioma cells under hypoxia remain unknown.\u0000\u0000\u0000MATERIAL AND METHODS\u0000Human glioma cell lines (LN229 and LN18) were transfected with HIF-1α or GLI1-specific short hairpin RNAs (shRNAs) and cultured under normoxic or hypoxic conditions. The protein levels of HIF-1α, GLI1, and epithelial-mesenchymal transition (EMT) markers including E-cadherin and vimentin were measured by Western blot analysis. RT-qPCR analysis was performed for the detection of HIF-1α and GLI1 mRNA expression. Cell migratory and invasive capacities were evaluated by wound healing and Transwell assays, respectively.\u0000\u0000\u0000RESULTS\u0000Hypoxia blocked the breakdown of the HIF-1α protein and upregulated GLI1 expression in glioma cells. Downregulation of HIF-1α expression inhibited hypoxia-induced cell migration and invasion, as well as reversed the effects of hypoxia on GLI1, E-cadherin, and vimentin expression in LN229 and LN18 cells. Depletion of GLI1 inhibited glioma cell migration and invasion induced by hypoxia. Silenced GLI1 did not affect HIF-1α expression but completely offset hypoxia-regulated expression of E-cadherin and vimentin in glioma cells.\u0000\u0000\u0000CONCLUSIONS\u0000GLI1 is involved in HIF-1α-induced migration, invasion, and EMT in glioma cells, thus revealing a novel molecular mechanism for glioma research.","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41787205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}