Correlation between stromal Th and Tc lymphocytes and PD-L1 expression in early breast cancer tumors.

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Folia histochemica et cytobiologica Pub Date : 2023-01-01 Epub Date: 2023-12-11 DOI:10.5603/fhc.97855
Jelena Vučinić, Ljiljana Vučković, Janja Raonić
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引用次数: 0

Abstract

Introduction: Prognostic and predictive value of PD-L1 as a biomarker in breast cancer remains controversial. While some studies suggest its association with negative prognostic parameters, others reported a highly significant association between PD-L1 expression and tumor-infiltrating lymphocytes, which are known to be an independent favorable prognostic factor. The aim of present study is to examine the relationship between immune response markers and PD-L1 expression in early breast cancer.

Material and methods: Immunohistochemical expression of PD-L1, along with density and composition of stromal lymphocytic infiltrate and peritumoral lymphoid aggregates was analyzed in 95 samples of invasive breast cancer.

Results: A strong positive correlation between PD-L1 expression and the density of stromal lymphocytic infiltrate and peritumoral lymphoid aggregates was identified and a cut-off value of 53% coverage of tumor stroma by lymphocytes, with which PD-L1 positivity can be predicted with excellent diagnostic accuracy, was determined for the first time using statistical methods. Additionally, PD-L1 positivity was observed significantly more often in tumors with higher absolute number of both CD4 and CD8 T-lymphocytes in the stromal infiltrate. No significant correlation with molecular subtype of breast cancer was found.

Conclusions: Our results indicate that the density of stromal lymphocytic infiltrate might be a better predictor of PD-L1 positivity in early breast cancer than the molecular subtype and that the key to the optimization of PD-L1 as a biomarker in breast cancer lies in its interpretation in the context of other immune response markers.

早期乳腺癌肿瘤中基质 Th 和 Tc 淋巴细胞与 PD-L1 表达之间的相关性。
导言一些研究表明PD-L1与预后不良的参数有关,而另一些研究则报道PD-L1表达与肿瘤浸润淋巴细胞之间存在高度显著的关联,而肿瘤浸润淋巴细胞是一个独立的有利预后因素。本研究旨在探讨早期乳腺癌中免疫反应标志物与 PD-L1 表达之间的关系:在 95 例浸润性乳腺癌样本中分析了 PD-L1 的免疫组化表达以及基质淋巴细胞浸润和瘤周淋巴聚集的密度和组成:结果:研究发现,PD-L1的表达与基质淋巴细胞浸润和瘤周淋巴聚集的密度呈强正相关,并首次利用统计学方法确定了肿瘤基质淋巴细胞覆盖率为53%的临界值,根据该临界值可预测PD-L1阳性,诊断准确性极高。此外,在基质浸润中 CD4 和 CD8 T 淋巴细胞绝对数量较高的肿瘤中,PD-L1 阳性的观察率明显更高。我们没有发现PD-L1阳性与乳腺癌分子亚型有明显的相关性:我们的研究结果表明,基质淋巴细胞浸润的密度可能比分子亚型更能预测早期乳腺癌的 PD-L1 阳性,优化 PD-L1 作为乳腺癌生物标志物的关键在于结合其他免疫反应标志物对其进行解释。
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来源期刊
Folia histochemica et cytobiologica
Folia histochemica et cytobiologica 生物-生化与分子生物学
CiteScore
2.80
自引率
6.70%
发文量
56
审稿时长
6-12 weeks
期刊介绍: "Folia Histochemica et Cytobiologica" is an international, English-language journal publishing articles in the areas of histochemistry, cytochemistry and cell & tissue biology. "Folia Histochemica et Cytobiologica" was established in 1963 under the title: ‘Folia Histochemica et Cytochemica’ by the Polish Histochemical and Cytochemical Society as a journal devoted to the rapidly developing fields of histochemistry and cytochemistry. In 1984, the profile of the journal was broadened to accommodate papers dealing with cell and tissue biology, and the title was accordingly changed to "Folia Histochemica et Cytobiologica". "Folia Histochemica et Cytobiologica" is published quarterly, one volume a year, by the Polish Histochemical and Cytochemical Society.
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