Fiziolohichnyĭ zhurnal最新文献_第4页

PATHOPHYSIOLOGICAL MECHANISMS OF DEEP VEIN THROMBOSIS 深静脉血栓形成的病理生理机制

Fiziolohichnyĭ zhurnal Pub Date : 2023-11-10 DOI: 10.15407/fz69.06.133

S. Chooklin, S. Chuklin

引用次数: 0

ANESTHETIC LIDOCAINE INHIBITS CHOLINERGIC CONTRACTION OF THE MOUSE SMALL INTESTINE AND CAUSES DISORDERS OF THE HUMAN GASTROINTESTINAL TRACT 麻醉剂利多卡因能抑制小鼠小肠的胆碱能收缩，并导致人类胃肠道功能紊乱

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.075

O.S. Savuliak, I.A. Sukha, D.О. Dziuba, M.I. Melnyk, D. Dryn

{"title":"ANESTHETIC LIDOCAINE INHIBITS CHOLINERGIC CONTRACTION OF THE MOUSE SMALL INTESTINE AND CAUSES DISORDERS OF THE HUMAN GASTROINTESTINAL TRACT","authors":"O.S. Savuliak, I.A. Sukha, D.О. Dziuba, M.I. Melnyk, D. Dryn","doi":"10.15407/fz69.05.075","DOIUrl":"https://doi.org/10.15407/fz69.05.075","url":null,"abstract":"Lidocaine is one of the components of multimodal low-opioid anesthesia, which is commonly used in surgical procedures, especially in cardiac surgery. Lidocaine has been used in medicine for a long time as a local anesthetic, but after the invention of the method of its intravenous administration, questions arose about its possible side effects on the visceral system, in particular on the motility of the gastrointestinal tract. Therefore, the aim of our work was to investigate the main aspects of the use of lidocaine-based opioid-free anesthesia and its side effects and also to study the effect of lidocaine on the contractile activity of small intestine smooth muscle, namely the mouse ileum. We recorded the contractile activity of smooth muscles of the ileum of mice using the tensiometry method and found that lidocaine at the clinically relevant concentration range, i.e. 1.5, 3, and 5 μg/ml, inhibited carbachol-induced contractions by 16, 27 and 37%, respectively (n = 7). To determine the side effects of opioid-free anesthesia, we studied 60 patients with coronary artery disease undergoing coronary artery stenting with the administration of anesthesia based on lidocaine solution at different concentrations (1 and 2 mg/kg). The side effects included nausea and vomiting, which may indicate а disturbance of gastrointestinal motility, as well as numbness of the limbs, cheeks, tongue, etc. These results contribute to a better understanding of the spectrum of action of lidocaine, a popular anesthetic in surgery, on the visceral system and become an important basis for the future development of new schemes for the use of local anesthetics in clinical practice, particularly in surgical procedures.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IMMUNOTHROMBOSIS AS A COMPONENT OF HOST DEFENCE 免疫血栓形成是宿主防御的一个组成部分

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.089

S. Chooklin, S. Chuklin

引用次数: 0

PROGESTERONE MODULATION OF ANXIETY AND DOPAMINERGIC MESOLIMBIC SYSTEM OF THE BRAIN ACTIVITY IN RATS WITH ALCOHOL DEPENDENCE AND UNDER CONDITIONS OF ZOOSOCIAL CONFLICT 黄体酮对酒精依赖和动物园社会冲突条件下大鼠焦虑和大脑多巴胺能中叶系统活动的调节作用

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.043

O. Berchenko, A. Shliakhova, O. Veselovska, A. Titkova, N. Levicheva

{"title":"PROGESTERONE MODULATION OF ANXIETY AND DOPAMINERGIC MESOLIMBIC SYSTEM OF THE BRAIN ACTIVITY IN RATS WITH ALCOHOL DEPENDENCE AND UNDER CONDITIONS OF ZOOSOCIAL CONFLICT","authors":"O. Berchenko, A. Shliakhova, O. Veselovska, A. Titkova, N. Levicheva","doi":"10.15407/fz69.05.043","DOIUrl":"https://doi.org/10.15407/fz69.05.043","url":null,"abstract":"Exogenous modulation by progesterone of the central neurosteroid mechanisms of regulation of anxiety and its important component, the activity of the mesolimbic dopaminergic system, is a promising method of correction of emotional behavioral disorders. The aim of this work was to study the effect of intranasal progesterone administration on the baseline level of anxiety and the activity of the dopaminergic mesolimbic brain system in alcohol-dependent and zoosocial conflict rats. Neuroethological studies by the method of assessing the individual level of anxiety. The levels of catecholamines (dopamine, noradrenaline, adrenaline) in the brain structures were determined by the method of immunoenzymatic analysis. Emotional stress, namely zoosocial instability and confrontational interactions, was modeled using the sensory contact technique and the partition test. Alcohol dependence in rats was induced by voluntary consumption of alcohol-containing food. Progesterone was administered intranasally at a dose of 80 μg per animal. It is shown that the level of anxiety in rats under conditions of alcohol dependence and emotional stress is associated with dopamine and adrenergic disturbances in the ventral tegmental area (VTA) and nucleus accumbens. The anxiolytic effects of progesterone are manifested in the suppression of the increase in anxiety following exposure to emotional stress in alcohol-dependent rats and its suppression in rats with baseline low levels of anxiety following repeated exposure to emotional stress. A balanced activation of the dopaminergic mesolimbic system during progesterone administration leads to the formation of a new allostatic state in rats with a decrease in anxiety levels.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE INFLUENCE OF TYROSINE PROTEIN KINASES BLOCKADE ON THE VASCULONEDOTHELIAL GROWTH FACTOR EXPRESSION AND DIABETIC RETINOPATHY DEVELOPMENT 酪氨酸蛋白激酶阻断剂对血管内皮生长因子表达和糖尿病视网膜病变发展的影响

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.22

S. Ziablitsev, V. Vodianyk, O. Dyadyk

{"title":"THE INFLUENCE OF TYROSINE PROTEIN KINASES BLOCKADE ON THE VASCULONEDOTHELIAL GROWTH FACTOR EXPRESSION AND DIABETIC RETINOPATHY DEVELOPMENT","authors":"S. Ziablitsev, V. Vodianyk, O. Dyadyk","doi":"10.15407/fz69.05.22","DOIUrl":"https://doi.org/10.15407/fz69.05.22","url":null,"abstract":"One of the main factors in the development of diabetic retinopathy (DR) is vasculoendothelial growth factor (VEGF), which is the end product of the activation of several intracellular signaling pathways, including those triggered by the activation of receptor tyrosine protein kinases. There is a need to justify new approaches to influence the expression of VEGF, not only in the late but also in the early stages of DR. Diabetes was modeled in 45 three-month-old male Wistar rats by a single injection of streptozotocin (50 mg/kg; Sigma- Aldrich, China). Hyperglycemia led to the development of early (on the 7–28th day) morphological manifestations of DR, indicating pronounced degenerative changes in nerve cells, microcirculation, and metabolism disorders. The use of insulin resulted in fewer diabetic changes in the retina, while the combined use of insulin and the tyrosine protein kinase blocker imatinib prevented the morphological manifestations of DR. According to the results of an immunohistochemical study, overexpression of VEGF was observed in the retinal tissue, which was inhibited by the introduction of insulin and, to a greater extent, by the combination of insulin with imatinib. According to immunoblotting results, the levels of VEGF and hypoxia-inducible factor (HIF-1) in the retinal tissue increased several-fold, which was significantly inhibited by insulin and prevented by insulin in combination with imatinib. Thus, this suggests that blockade of tyrosine protein kinases may be a highly effective way of preventing or correcting the damage caused by DR.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STRESS AND HYPERTENSION IN WAR AND COVID-19 CONDITIONS 战争和科维德-19 条件下的压力和高血压

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.100

L.M. Samokhina, Yu.S. Rudyk

{"title":"STRESS AND HYPERTENSION IN WAR AND COVID-19 CONDITIONS","authors":"L.M. Samokhina, Yu.S. Rudyk","doi":"10.15407/fz69.05.100","DOIUrl":"https://doi.org/10.15407/fz69.05.100","url":null,"abstract":"Common mechanisms of the development of the body’s stress response under the conditions of war and the COVID-19 pandemic relate to its acute and chronic course, post-traumatic stress disorder, the role of the hypothalamus-pituitaryadrenal gland, the sympathetic nervous system, and the renin-angiotensin-aldosterone system, and deficiency of α-1- antitrypsin. The development of stress-induced hypertensive changes is characterized by the activity of enzymes independent of the angiotensin-converting enzyme pathway of the renin-angiotensin system, expression of miRNA, damage to the endothelial cells of blood vessels, changes in the content of neutrophils and lymphocytes, and the soluble receptor for glycosylation end products. The considered stress-induced hypertensive changes under the conditions of the COVID-19 pandemic relate to chronic hyperinflammation, emotional response, effects of catecholamines, chymase activity, redox imbalance, DNA damage, endoplasmic reticulum stress. The interaction between ACE/ACE2 and miRNA may increase the probability of SARS-CoV-2 infection in patients with hypertension. A factor in the severity of COVID-19 is considered to be clinical depression and low immunity, and the possibility of a negative effect of antidepressants is highlighted. A more aggressive course of COVID-19 in hypertensive patients is associated with low levels of nitric oxide (NO). Stress-induced hypertensive changes under the conditions of war are associated with adaptation disorders, increased randomness of heart rate changes, post-traumatic stress disorder, the development of hypertension partly of psychological origin against the background of changes in the central nervous system, immunity, and the polymorphism of the ACEI/D gene as an indicator of endurance.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FEATURES OF APPLICATION OF PSYCHOPHYSIOLOGICAL PROFILES OF OPERATORS OF UNMANNED AVIATION COMPLEXES FOR THEIR OCCUPATIONAL SELECTION 应用无人驾驶航空综合体操作员的心理生理特征进行职业选择的特点

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.003

V. Kalnysh, A.V. Shvets, O.V. Maltsev, S. Pashkovskyi, N. V. Koval

{"title":"FEATURES OF APPLICATION OF PSYCHOPHYSIOLOGICAL PROFILES OF OPERATORS OF UNMANNED AVIATION COMPLEXES FOR THEIR OCCUPATIONAL SELECTION","authors":"V. Kalnysh, A.V. Shvets, O.V. Maltsev, S. Pashkovskyi, N. V. Koval","doi":"10.15407/fz69.05.003","DOIUrl":"https://doi.org/10.15407/fz69.05.003","url":null,"abstract":"In order to develop new approaches to the psychophysiological selection of operators, 219 male servicemen aged 20 to 40 years old with experience in operating unmanned aerial systems of the first class Light and involved in a wide range of professional tasks were examined. To study their psychophysiological status, modified methods were used using the PFI-2 hardware and software complex. The following indicators were determined: accuracy of reaction to a moving object, strength of nervous processes, functional mobility of nervous processes, simple visual-motor reaction, complex visual-motor reaction, orientation in space, and visual memory. To identify a set of informative indicators that can best divide the analyzed group of operators into 3 clusters, a stepwise discriminant analysis was applied. The technique was developed to obtain several psychophysiological profiles of vocational aptitude, equally effective in terms of content. The statistical discrepancy of these profiles has been established. Ways of evaluating the superiority of these profiles have been shown. The principle of “necessary diversity” in the implementation of occupational psychophysiological selection of operators working in conditions with increased danger has been proposed and discussed. A number of advantages of using this principle to increase accuracy and expand the contingent of persons subject to occupational psychophysiological selection have been identified. The technology of graphical presentation of psychophysiological profiles of operators of unmanned aerial complexes was developed for the purpose of assessment of suitability for professional tasks.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECT OF ACTOVEGYN ON THE MECHANISMS OF OXIDATIVE STRESS DEVELOPING IN PATIENTS WITH TYPE 2 DIABETES MELLITUS AND CARDIOVASCULAR AUTONOMIC NEUROPATHY Actovegyn 对 2 型糖尿病和心血管自主神经病变患者氧化应激机制的影响

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.012

Y. Saenko, O. Gonchar, I. Mankovska, T. Drevytska, L. V. Bratus, B.М. Mankovsky

{"title":"THE EFFECT OF ACTOVEGYN ON THE MECHANISMS OF OXIDATIVE STRESS DEVELOPING IN PATIENTS WITH TYPE 2 DIABETES MELLITUS AND CARDIOVASCULAR AUTONOMIC NEUROPATHY","authors":"Y. Saenko, O. Gonchar, I. Mankovska, T. Drevytska, L. V. Bratus, B.М. Mankovsky","doi":"10.15407/fz69.05.012","DOIUrl":"https://doi.org/10.15407/fz69.05.012","url":null,"abstract":"The effects of actovegin on the mechanisms of oxidative stress (OS) developing in the blood of patients with type 2 diabetes mellitus (DM2) and cardiovascular autonomic neuropathy (CVAN) were investigated. The aim of the study was to establish the effectiveness of treatment with actovegin for the pro- and antioxidant balance impairment and changes in gene expression of HIF-1α and mTOR in the blood of patients with DM2 and CVAN. It was shown that intravenous injections of actovegin at a dose of 1000 mg per day for 10 days and further prolonged oral administration of this drug at a dose of 800 mg per day for 90 days led to a decrease in the content of secondary products of lipid peroxidation in blood plasma and H2O2 production in erythrocytes of patients with DM2 and CVAN. These changes were indicative of a weakening of OS intensity. It was also shown that treatment with actovegin promoted an increase in total plasma SOD activity as well as reduced glutathione and glutathione peroxidase activity in erythrocytes from patients. Treatment with actovegin also raised the gene expression of HIF-1α and reduced the gene expression of mTOR in leukocytes of patients with DM2 and CVAN. These genetic changes may serve as a protective mechanism against the development of OS, which acts through different metabolic pathways. So, actovegin administration counteracting OS development due to the impact on the different components of pro- and antioxidant system as well as on HIF-1α and mTOR genes expression may offer new clinical avenues for pharmacological treatment of patients with DM2 and CVAN.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"151 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETECTION OF ANTIBODIES TO SARS-COV-2 VIRUS IN PATIENTS WITH CORONARY HEART DISEASE AND SYSTEMIC RHEUMATIC DISEASES BEFORE THE ONSET OF THE COVID-19 PANDEMIC 在科维德-19（COVID-19）病毒大流行前检测冠心病和系统性风湿病患者体内的萨斯-科维德-2（SARS-COV-2）病毒抗体

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.061

V. Kornatskyi, N. O. Ryzhkova, O.M. Parkhomenko, O. Lomakovsky, A. M. Dorokhina, Yu.V. Kornatskyi, V.G. Levchenko, A.V. Romanovskyi

引用次数: 0

VIABILITY AND TYPES OF DEATH OF BLOOD LEUKOCYTES IN RATS WITH POLYCYSTIC OVARY SYNDROME UNDER CONDITIONS OF INTERMITTENT COLD EXPOSURE 多囊卵巢综合征大鼠在间歇性冷暴露条件下血白细胞的活力和死亡类型

Fiziolohichnyĭ zhurnal Pub Date : 2023-10-05 DOI: 10.15407/fz69.05.033

M. Zhulikova, M. Myroshnychenko, O.A. Nakonechna, O. O. Zhulikov, V. O. Bibichenko, S. Myroshnychenko, O.V. Zaytseva, M. Kovaltsova

{"title":"VIABILITY AND TYPES OF DEATH OF BLOOD LEUKOCYTES IN RATS WITH POLYCYSTIC OVARY SYNDROME UNDER CONDITIONS OF INTERMITTENT COLD EXPOSURE","authors":"M. Zhulikova, M. Myroshnychenko, O.A. Nakonechna, O. O. Zhulikov, V. O. Bibichenko, S. Myroshnychenko, O.V. Zaytseva, M. Kovaltsova","doi":"10.15407/fz69.05.033","DOIUrl":"https://doi.org/10.15407/fz69.05.033","url":null,"abstract":"The aim of our work is to assess the viability and determine the types of death of blood leukocytes in rats with polycystic ovary syndrome (PCOS) under conditions of intermittent cold exposure. The study was performed on 40 immature female rats of the WAG population, aged 27 days, divided into 5 groups of 8 animals each. Group 1 included intact rats. In group 2, rats were subcutaneously injected with 0.2 ml of purified and sterilized olive oil daily for 25 days. In group 3, rats were exposed to periodic cold exposure daily for 25 days. In group 4, dehydroepiandrosterone-induced PCOS was modeled in rats. In group 5, rats were injected with dehydroepiandrosterone against the background of periodic cold exposure. Leukocyte viability and death pathways were determined using a FACS Canto II flow cytometer (Becton Dickinson, USA). Rats with dehydroepiandrosteroneinduced PCOS showed a decrease in the percentage of viable leukocytes and an increase in the percentage of leukocytes in the early stage of apoptosis, while the number of leukocytes in the late stage of apoptosis and necrotic leukocytes did not change. In rats, on the background of the introduction of dehydroepiandrosterone under conditions of intermittent cold exposure, the percentage of viable leukocytes, as well as leukocytes at different stages of apoptosis and necrosis, corresponded to the physiological norm. Thus, the authors revealed a decrease in the viability of neutrophilic leukocytes in the blood of rats with dehydroepiandrosterone-induced PCOS due to the activation of apoptosis processes, as evidenced by an increase in the percentage of leukocytes at the initial stages of apoptosis. Intermittent cold exposure (4 h daily at +4°C for 25 days) against the background of dehydroepiandrosterone administration to rats had an anti-apoptotic effect, normalizing the viability of neutrophilic blood leukocytes.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"58 3-4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0