多囊卵巢综合征大鼠在间歇性冷暴露条件下血白细胞的活力和死亡类型

M. Zhulikova, M. Myroshnychenko, O.A. Nakonechna, O. O. Zhulikov, V. O. Bibichenko, S. Myroshnychenko, O.V. Zaytseva, M. Kovaltsova
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摘要

我们的工作旨在评估多囊卵巢综合症(PCOS)大鼠在间歇性寒冷暴露条件下血液白细胞的存活率并确定其死亡类型。研究对象是 40 只年龄为 27 天的 WAG 未成熟雌性大鼠,分为 5 组,每组 8 只。第 1 组包括完整的大鼠。在第 2 组中,每天向大鼠皮下注射 0.2 毫升纯化灭菌橄榄油,连续 25 天。在第 3 组中,每天对大鼠进行周期性冷暴露,持续 25 天。第 4 组是脱氢表雄酮诱导的多囊卵巢综合征模型。在第 5 组中,给大鼠注射脱氢表雄酮,同时进行周期性寒冷暴露。使用 FACS Canto II 流式细胞仪(Becton Dickinson,美国)测定白细胞活力和死亡途径。脱氢表雄酮诱导的多囊卵巢综合征大鼠有活力的白细胞百分比下降,处于凋亡早期的白细胞百分比增加,而处于凋亡晚期的白细胞和坏死白细胞的数量没有变化。在大鼠间歇性受冷条件下引入脱氢表雄酮的背景下,有活力的白细胞以及处于凋亡和坏死不同阶段的白细胞的百分比与生理正常值相符。因此,作者发现脱氢表雄酮诱导的多囊卵巢综合征大鼠血液中嗜中性白细胞的存活率下降,原因是凋亡过程被激活,处于凋亡初始阶段的白细胞百分比增加就是证明。在给大鼠注射脱氢表雄酮的背景下,间歇性冷暴露(每天 4 小时,+4°C,持续 25 天)具有抗凋亡作用,使嗜中性粒细胞血液白细胞的活力恢复正常。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
VIABILITY AND TYPES OF DEATH OF BLOOD LEUKOCYTES IN RATS WITH POLYCYSTIC OVARY SYNDROME UNDER CONDITIONS OF INTERMITTENT COLD EXPOSURE
The aim of our work is to assess the viability and determine the types of death of blood leukocytes in rats with polycystic ovary syndrome (PCOS) under conditions of intermittent cold exposure. The study was performed on 40 immature female rats of the WAG population, aged 27 days, divided into 5 groups of 8 animals each. Group 1 included intact rats. In group 2, rats were subcutaneously injected with 0.2 ml of purified and sterilized olive oil daily for 25 days. In group 3, rats were exposed to periodic cold exposure daily for 25 days. In group 4, dehydroepiandrosterone-induced PCOS was modeled in rats. In group 5, rats were injected with dehydroepiandrosterone against the background of periodic cold exposure. Leukocyte viability and death pathways were determined using a FACS Canto II flow cytometer (Becton Dickinson, USA). Rats with dehydroepiandrosteroneinduced PCOS showed a decrease in the percentage of viable leukocytes and an increase in the percentage of leukocytes in the early stage of apoptosis, while the number of leukocytes in the late stage of apoptosis and necrotic leukocytes did not change. In rats, on the background of the introduction of dehydroepiandrosterone under conditions of intermittent cold exposure, the percentage of viable leukocytes, as well as leukocytes at different stages of apoptosis and necrosis, corresponded to the physiological norm. Thus, the authors revealed a decrease in the viability of neutrophilic leukocytes in the blood of rats with dehydroepiandrosterone-induced PCOS due to the activation of apoptosis processes, as evidenced by an increase in the percentage of leukocytes at the initial stages of apoptosis. Intermittent cold exposure (4 h daily at +4°C for 25 days) against the background of dehydroepiandrosterone administration to rats had an anti-apoptotic effect, normalizing the viability of neutrophilic blood leukocytes.
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