Fiziolohichnyi zhurnal最新文献_第9页

[Disturbances of oxygen-dependent processes in periodontal tissues under prolonged immobilization stress and ways of their pharmacological correction]. [长时间固定应激对牙周组织氧依赖过程的干扰及其药物纠正方法]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.01.017

H. B. Opanasenko, L. V. Bratus, B. L. Havenauskas, O. Honchar, I. M. Man'kovs'ka, V. Nosar, S. B. Frantsuzova

{"title":"[Disturbances of oxygen-dependent processes in periodontal tissues under prolonged immobilization stress and ways of their pharmacological correction].","authors":"H. B. Opanasenko, L. V. Bratus, B. L. Havenauskas, O. Honchar, I. M. Man'kovs'ka, V. Nosar, S. B. Frantsuzova","doi":"10.15407/FZ59.01.017","DOIUrl":"https://doi.org/10.15407/FZ59.01.017","url":null,"abstract":"Influence of prolonged immobilization (6 h strict horizontal position of rats in the tight containers daily for 2 weeks) on oxygen tension, oxygen consumption, pro-/antioxidant balance, and energetic metabolism of soft and hard periodontal tissues has been investigated. It was established that prolonged immobilization stress resulted in marked decrease in the gum tissue PO2 (36%) and in the bone tissue oxygen consumption rate (46%) compared to control. It was also determined that prolonged stress led to a reduction in the gum mitochondrial respiration rate. The latter was more expressed in case of the NAD-dependent substrate oxidation than of the FAD- dependent one. It was determined that the prolonged stress results in intensification of peroxide processes and depletion of antioxidant protection of soft tissues of periodontum. It was found that Thiotriazolin and Actovegin have modified and diminished stress-induced disorders in the soft and hard periodontal tissues oxygen homeostasis under prolonged immobilization stress.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75490703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[The influence of activation of the ATP-sensitive potassium channels by flocalin on the function of the cardiovascular system]. [flocalin激活atp敏感钾通道对心血管系统功能的影响]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.01.011

R. B. Strutyns'kyĭ, R. A. Rovenets, N. A. Strutyns'ka, O. P. Neshcheret, O. Moibenko

{"title":"[The influence of activation of the ATP-sensitive potassium channels by flocalin on the function of the cardiovascular system].","authors":"R. B. Strutyns'kyĭ, R. A. Rovenets, N. A. Strutyns'ka, O. P. Neshcheret, O. Moibenko","doi":"10.15407/FZ59.01.011","DOIUrl":"https://doi.org/10.15407/FZ59.01.011","url":null,"abstract":"In experiments on the anaesthetized dogs the influence of a new fluorine-containing opener of ATP-sensitive potassium (K(ATP)) channels flocalin on the cardiohemodynamic of great animals in vivo was studied. Flocalin introduced intravenously in doses 0.01 - 1.5 mgs/kg. It is shown that it reduces in dose-dependent manner a system arterial pressure, perfusion pressure in coronary artery and general peripheral resistance of vessels with maximal effects on 56.8 +/- 2.7, 22.4 +/- 4.7 and 47.2% +/- 6.5% accordingly at most dose 1.5 mgs/kg. Flocalin causes development of cardiodepressive reactions in heart, that is exhibited in dose-dependent the decrease of pressure in the left ventricle, speed of growth (dP/dt(max)) and reduction (dP/dt(min)) in it's of pressure with maximal effects on 37.1 +/- 5.1, 51.2 +/- 9.4 and 55.6% +/- 6.9% accordingly at introduction of most dose of flocalin. Diminish of the cardiac out put and heart rate with a maximal effects on 23.1% +/-12.7% and 19.2% +/- 1.7% accordingly at a dose 1.0 mgs/kg was shown. It should be noted that considerable reduction of heart rate and general peripheral resistance of vessels takes place only at the large doses of flocalin - 1 and 1.5 mgs/kg. Thus, it is shown that activation of K(ATP) channels by flocalin causes the dose-dependent decrease of pressure in the system of circulation of blood and contraction activity of myocardium.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74353522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

[Prophylactic effect of probiotic strains Bifidobacterium animalis VKL and VKB on stress-induced lesions in the gastric mucosa of rats]. [动物双歧杆菌VKL和VKB对应激性大鼠胃粘膜病变的预防作用]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V5.I1.80

M. Spivak, L. Lazarenko, T. Falalieieva, O. Virchenko, K. Neporada

引用次数: 6

[Stress-induced changes in the content of cytochrome P450 2E1 in the liver of mice with chronic psychoemotional overexertion]. 慢性精神情绪过度劳累小鼠肝脏细胞色素P450 2E1含量的应激性变化

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.04.067

Maksymchuk Ov, Chashchyn Mo

引用次数: 0

[The effect of Ca2+ on the properties of the large conductance cation channels of the nuclear envelope of the cerebellar neurons]. [Ca2+对小脑神经元核膜大电导阳离子通道性质的影响]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V5.I3.20

O. V. Lun'ko, O. Fedorenko, S. Marchenko

引用次数: 2

[Endoplasmic reticulum stress and angiogenesis]. 内质网应激与血管生成。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.04.093

D. Minchenko, K. Kubaichuk, O. V. Hubenia, I. V. Kryvdiuk, I. Khomenko, R. M. Herasymenko, R. Sulik, N. Murashko, O. Minchenko

{"title":"[Endoplasmic reticulum stress and angiogenesis].","authors":"D. Minchenko, K. Kubaichuk, O. V. Hubenia, I. V. Kryvdiuk, I. Khomenko, R. M. Herasymenko, R. Sulik, N. Murashko, O. Minchenko","doi":"10.15407/FZ59.04.093","DOIUrl":"https://doi.org/10.15407/FZ59.04.093","url":null,"abstract":"The endoplasmic reticulum is a dynamic intracellular organelle with exquisite sensitivity to alterations in homeostasis, and provides stringent quality control systems to ensure that the only correctly folded proteins transit to the Golgi and unfolded or misfolded proteins are retained and ultimately degraded. The endoplasmic reticulum stress represents the unfolded protein response to cope with the accumulation of unfolded or misfolded proteins and is required to maintain the functional integrity of the endoplasmic reticulum. The endoplasmic reticulum stress is a fundamental phenomenon which provides a secure protection of the cells from different factors. This stress provides a wide spectrum of physiological roles in diverse developmental and metabolic processes, especially for professional secretory cells with high-level secretory protein synthesis, such as pancreatic beta cells, hepatocytes and osteoblasts and is required throughout the entire life. The endoplasmic reticulum stress and hypoxia are the obligate components of malignant tumor growth, are interconnected and activate angiogenesis via growth and metabolism control. The endoplasmic reticulum stress is mediated by three by three sensor and signaling pathways (PERK, ATF6 and ERN1), besides that blockade one (ERN1) leads to a decrease of tumor growth through suppression of angiogenesis and proliferation. The data concerning the interaction of signaling enzyme ERN1 and pro- and anti-angiogenic gene expressions is analyzed.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89649930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

[Ionic mechanisms of carbon monoxide action on the contractile properties of smooth muscles of the blood vessels]. [一氧化碳作用于血管平滑肌收缩特性的离子机制]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.03.018

M. B. Baskakov, A. Zheludeva, S. Gusakova, L. Smaglii, A. Aleinik, P. I. Ianchuk, M. Medvedev, S. Orlov

{"title":"[Ionic mechanisms of carbon monoxide action on the contractile properties of smooth muscles of the blood vessels].","authors":"M. B. Baskakov, A. Zheludeva, S. Gusakova, L. Smaglii, A. Aleinik, P. I. Ianchuk, M. Medvedev, S. Orlov","doi":"10.15407/FZ59.03.018","DOIUrl":"https://doi.org/10.15407/FZ59.03.018","url":null,"abstract":"Carbon monoxide (CO) is one of a family of gas transmitters. In this article we present the results of mechanographic investigations of the mechanisms of CO action on a rat thoracic aorta segments. We found that relaxing effect of CO donor CORM-2 on vascular smooth muscles is mediated mainly by opening of voltage-dependent potassium channels in smooth muscle cells: 4-aminopyridine, blocking these channels, almost completely eliminated the CO-induced vasorelaxation of the segments precontracted by depolarization of the smooth muscle cells membranes with high potassium (30 mM KCl) solution or by phenylephrine (10 microM). For the first time we documented that CORM-2 reduces the nicardipine-sensitive input of 45Ca2+ in freshly isolated aorta cells. There are reasons to suggest that the L-type voltage-dependent calcium channels of vascular smooth muscle cells are another target for CO, which is implemented in the relaxing effect of this gas transmitter. Additional research is needed to determine the influence of ruthenium complexes (Ru(II)) on phenomenology of carbon monoxide effects.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86798783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Hydrogen sulfide inhibits Ca(2+)-induced mitochondrial permeability transition pore opening in spontaneously hypertensive rats]. [硫化氢抑制Ca(2+)诱导的自发性高血压大鼠线粒体通透性过渡孔打开]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.01.003

Strutyns'ka Na, Dorofeieva No, Vavilova Hl, Sahach Vf

{"title":"[Hydrogen sulfide inhibits Ca(2+)-induced mitochondrial permeability transition pore opening in spontaneously hypertensive rats].","authors":"Strutyns'ka Na, Dorofeieva No, Vavilova Hl, Sahach Vf","doi":"10.15407/FZ59.01.003","DOIUrl":"https://doi.org/10.15407/FZ59.01.003","url":null,"abstract":"In experiments in vivo and in vitro on the mitochondria isolated from the control and spontaneously hypertensive rats (SHR) hearts, we studied the effects of a donor of hydrogen sulfide (H2S), NaHS, and H2S biosynthesis substrate, L-cysteine, on the sensitivity of the mitochondrial permeability transition pore (mPTP) opening to its natural inductor, Ca2+. We found that NaHS (10(-4), 10(-5) and 5 10(-5) mol/l) influenced the mitochondrial swelling in a concentration-dependent manner in control and spontaneously hypertensive rats. The H2S donor NaHS used in physiological concentrations (10(-6), 10(-5) and 5 10(-5) mol/l) exerted the inhibiting effect on the Ca(2+)-induced mPTP opening in control hearts (corresponding values of such effect were 31, 76, and 100%, respectively), while in spontaneously hypertensive rats hearts the protector effect of NaHS was observed only at its concentration of 10(-5) - 10(-4) mol/l. In experiments in vivo, single intraperitoneal injections of L-cysteine (10(-3) mol/kg) resulted in a decrease in the sensitivity of mPTP to it's inductor Ca2+ in control rats and SHR. In experiments in vivo in which we used a specific blocker of cystathionine-gamma-lyase, propargylglycine (10(-4) mol/kg), with the further injections of L-cysteine we observed a decrease in the threshold Ca2+ concentration (that induce the mitochondrial swelling) by three orders of magnitude in SHR, but in control rats did not effect of L-cysteine. Thus, both endogenous and exogenous hydrogen sulfide inhibits Ca(2+)-induced mitochondrial permeability transition pore opening, indicating its protective effect on pore formation in spontaneously hypertensive rats hearts. Therefore, our studies are indicative of the involvement of H2S in modulation of changes in the permeability of mitochondrial membranes, which can be an important regulatory factor in the development of cardiovascular diseases.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75948859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

[The role of NADPH-oxidase in paracrine and autocrine regulation of platelet functional activity]. [nadph氧化酶在旁分泌和自分泌调节血小板功能活性中的作用]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.05.085

S. A. Talanov, T. I. Liashenko, I. I. Patalakh

引用次数: 0

[Effect of hydrogen sulfide donor NaHs on the functional state of the respiratory chain of the rat heart mitochondria]. [硫化氢供体NaHs对大鼠心脏线粒体呼吸链功能状态的影响]。

Fiziolohichnyi zhurnal Pub Date : 2013-01-01 DOI: 10.15407/FZ59.02.009

O. M. Semenykhina, N. A. Strutyns'ka, A. I. Bud'ko, H. L. Vavilova, V. F. Sahach

{"title":"[Effect of hydrogen sulfide donor NaHs on the functional state of the respiratory chain of the rat heart mitochondria].","authors":"O. M. Semenykhina, N. A. Strutyns'ka, A. I. Bud'ko, H. L. Vavilova, V. F. Sahach","doi":"10.15407/FZ59.02.009","DOIUrl":"https://doi.org/10.15407/FZ59.02.009","url":null,"abstract":"In experiments on mitochondria isolated from the heart tissue of adult rats we studied the effects of a donor of hydrogen sulfide, NaHS, on the respiratory chain of the organelles. We found that NaHS (10(-9)-10(-6) mol/l) caused a dose-dependent decrease in the rate of oxygen consumption in the presence of succinate and ADP (state 3 to Chance), and in the absence of ADP (state 4). The decrease in the rate of oxygen consumption in a concentration NaHS 10(-9) mol/l and 10(-8) mol/l associated with an increased conjugation of oxidation and phosphorylation, as evidenced by the increase in the respiratory control, the efficiency of oxidative phosphorylation (ADP/O) is not changed. Our studies suggest a protective effect of hydrogen sulfide donor on the functional state of the mitochondria. To elucidate of other the mechanisms of the protective action H2S we also investigated the effect of hydrogen sulfide donor on the mitochondrial swelling. It was found that NaHS in the range of concentration 10(-12) - 10(-4) mol/l influences the level of mitochondria swelling of the rats heart in the dose-dependent manner. It was also shown that when the concentration of Ca2+ 1 nmol/mg protein in the medium, under the action of hydrogen sulfide in the donor concentration range 10(-12) - 10(-8) mol/l, there was a moderate swelling of rats heart mitochondria. Under the action of NaHS at a concentration of 10(-9) mol/l it was observed swelling of the mitochondria, the maximum change in the level of which was 11%. Inhibitor of mitochondrial ATP-sensitive K+ channels (K(ATP) channels) 5-hydroxydecanoate (10(-4) mol/l) partially reduced the mitochondrial swelling in the presence of NaHS (10(-9) mol/l), which may indicate the activation of K(ATP) channels. Our studies point for possible involvement of mitochondrial K(ATP) channels in implementation of the mechanisms of H2S.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87872225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3