Fiziolohichnyi zhurnal最新文献_第8页

[The effect of enzymatic treatment using proteases on properties of persistent sodium current in CA1 pyramidal neurons of rat hippocampus]. [蛋白酶对大鼠海马CA1锥体神经元持续钠电流特性的影响]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/fz60.03.075

O. O. Lun’ko, D. S. Isaiev, O. P. Maxymiuk, O. O. Kryshtal', O. V. Isaieva

引用次数: 2

[Propargylglycine restores endothelium-dependent relaxation of aortic smooth muscles in old rats]. 丙基甘氨酸恢复老龄大鼠主动脉平滑肌内皮依赖性松弛。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I3.40

K. Drachuk, A. Kotsiuruba, O. V. Baziliuk, L. H. Stepanenko, V. F. Sahach

引用次数: 2

[The influence of amylin on the bile acid spectrum in rats]. [胰淀素对大鼠胆汁酸谱的影响]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/FZ60.03.046

I. P. Vasheka, S. P. Vesel'skyĭ, Z. A. Horenko, O. A. Hrinchenko, L. S. Karbovs'ka, M. Makarchuk

{"title":"[The influence of amylin on the bile acid spectrum in rats].","authors":"I. P. Vasheka, S. P. Vesel'skyĭ, Z. A. Horenko, O. A. Hrinchenko, L. S. Karbovs'ka, M. Makarchuk","doi":"10.15407/FZ60.03.046","DOIUrl":"https://doi.org/10.15407/FZ60.03.046","url":null,"abstract":"In acute experiments on the rats with cannulated common biliary duct, the influence ofamylin on the level of bile secretion and bile acids spectrum was investigated. It was shown that subcutaneous administration of amylin at the dose 1 mg/kg body weight doesn't affect the volume of secreted bile. Under these conditions, the concentration of taurocholic acid was increased and the concentration of tauroconjugates of chenodeoxycholic and deoxycholic acids was decreased in the bile. At the same time, the concentration of glycocholates remained constant and of unconjugated bile acids was decreased. This redistribution of bile acids spectrum leads to an increase in the coefficient of conjugation. Amylin changes the ratio of trygydroxy- and dygydroxycholates in secreted bile leading to an increase in the coefficient of hydroxylation. These results suggest that amylin enhances the processes of conjugation and hydroxylation of bile acids in hepatocytes that results in improvement of detergent properties of the bile, particularly, the ability of the bile to maintain the cholesterol in dissolved state. At the lowest effective dose, amylin does not alter the concentration of glucose in the blood.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76950650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[The effect of melatonin on bone tissue metabolism]. 褪黑素对骨组织代谢的影响。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I2.90

I. Litovka, Y. O. Mazepa-Kryzhanivs'ka, V. Berezovskyi

引用次数: 2

[Glycoprotein sialylation and NEU1 and ST6GAL1 expressions in erythremia]. [红血病中糖蛋白唾液化与NEU1和ST6GAL1的表达]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/FZ60.05.014

H. Maslak, O. Kostiuk, D. Minchenko, O. Brazaluk, A. Shevtsova, O. Minchenko

{"title":"[Glycoprotein sialylation and NEU1 and ST6GAL1 expressions in erythremia].","authors":"H. Maslak, O. Kostiuk, D. Minchenko, O. Brazaluk, A. Shevtsova, O. Minchenko","doi":"10.15407/FZ60.05.014","DOIUrl":"https://doi.org/10.15407/FZ60.05.014","url":null,"abstract":"We studied the levels of lymphocyte surface-associated sialylglycans and the expression of neuraminidase NEU1 and sialyltransferase ST6GAL1 genes in lymphocytes in erythremia patients and healthy donors as well as the levels of sialic acids in plasma and sialylation of alpha-acid glycoprotein and fibronectin. Moreover, we also investigated the type of sialic acids binding with its glycans using sialospecific lectins MAA and SNA. fibronectin protein in lymphocytes and its cell surface in erythremia disease as compared to healthy donors. It was shown that the levels of free sialic acids and neuraminidase activity in plasma are increased in erythremia disease as compared to healthy donors; however, MAA-II-binding activity of tested glycoproteins is decreased, fibronectin-1 mRNA expression in lymphocytes is increased in patients with erythremia. The decreasing of plasma fibronectin concentration and its heparin-binding activity as well as increasing of lymphocyte content with surface-associated and intracellular fibronectin were revealed in erythremia disease in comparison with healthy donors. Positive correlation between plasma fibronectin level and its heparin-binding activity and negative correlation between plasma fibronectin level and quantity of lymphocytes which express fibronectin inside the cell and on cell surface was detected. Enhanced levels of α2,3- and α2,6-linked residues of glycocojugates were detected on lymphocyte cell surface in erythremia disease using sialospecific lectins and flow cytometer as well as fluorescent confocal microscope. The level of NEU1 and ST6GAL1 mRNA expressions is significantly increased in lymphocytes in erythremia disease. Results of this study are clarified the mechanisms of disturbed in erythremia disease glycobiological processes and may therefore present new approaches for therapeutic opportunities.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84583933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[Parameters of NO synthase system of gastric mucosa in rats under stress conditions and inhibition of cyclooxygenase]. [应激条件下大鼠胃黏膜NO合酶系统参数及环加氧酶抑制]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/FZ60.02.051

I. Fomenko, T. Bondarchuk, L. P. Bilets'ka, N. B. Panasiuk, O. Skliarov

{"title":"[Parameters of NO synthase system of gastric mucosa in rats under stress conditions and inhibition of cyclooxygenase].","authors":"I. Fomenko, T. Bondarchuk, L. P. Bilets'ka, N. B. Panasiuk, O. Skliarov","doi":"10.15407/FZ60.02.051","DOIUrl":"https://doi.org/10.15407/FZ60.02.051","url":null,"abstract":"In experiments on rats with modeled water-restrained stress, the influence of nonsteroidal anti-inflammatory drugs of different genesis on morphological status of gastric mucosa and changes of NO-synthase system parameters have been studied Administration of nonselective cyclooxygenese inhibitor naproxen in the water-restrained stress model in rats potentiated the increase of severity of damage of gastric mucosa. At the same time, the activity of both inducible and constitutive isoforms ofNO-sythase decreased. The parameters of lipoperoxidation remained at the level observed during water-restrained stress. It was shown the advantages of the use of H2S-releasinfg nonsteroidal anti-inflammatory drug ATB-346, which are associated with its cytoprotective effect of the drug manifested by a decreased total area of gastric damage. However, parameters of lipoperoxidation and NO-syntase system did not differ substantially from those in the group treated with napoxen, indicating the prevalence of parent molecule (naproxen) in regulation of function of NO-system Administration of dual COX/LOX inhibitor, the compound 2A5DHT, caused a decrease of gastric damage as compared to the effect ofnaproxen. The activity of iNOS remained much higher than under condition of the naproxen action.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89586881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

[H+-Ca2+-exchanger in the myometrium mitochondria: modulation of exogenous and endogenous compounds]. [肌层线粒体中的H+-Ca2+交换剂:外源性和内源性化合物的调节]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I4.30

O. Kolomiiets, Iu V Danylovych, H. V. Danylovych

{"title":"[H+-Ca2+-exchanger in the myometrium mitochondria: modulation of exogenous and endogenous compounds].","authors":"O. Kolomiiets, Iu V Danylovych, H. V. Danylovych","doi":"10.1615/INTJPHYSPATHOPHYS.V6.I4.30","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V6.I4.30","url":null,"abstract":"The properties of ΔpH-induced Ca2+-transport from isolated rat myometrium mitochondria was investigated. Ca2+-accu- mulation was carried out in the presence of Mg-ATP2- and succinate. Transport of Ca2+ recorded using Ca2+-sensitive fluorescent probe Fluo-4 AM. It is shown that acidification of extramitochondrial medium is accompanied by stimulation of Ca2+ release from mitochondria. This process is insensitive to the tetraphenylphosphonium which is relatively specific Na+-Ca2+-exchanger inhibitor of mitochondrial inner membrane, but inhibited in the presence of monoclonal antibodies directed against the protein LETM1 (Anti-LETM1). LETM1 protein in some tissues is the molecular basis of the H+-Ca2+-exchanger functioning on mitochondria. It was found that the H+-Ca2+-exchanger is stimulated by 100 μM amiloride (diuretic) and inhibited by Mg ions in milimolar concentrations. The transport system was completely resistant to the action of nitric oxide (sodium nitroprusside and sodium nitrite), but was stimulated by macrocyclic compounds of Calixarenes (C-97 and C-99) in submicromolar concentrations. Thus, the mitochondria of rat myometrium probably not have a system of Na+-Ca2+-exchanger, and provide the maintenance of the matrix Ca2+-homeostasis with H+-Ca2+-exchanger. Since the transport system high affinity activated by Calixarenes, further investigation of the influence of these compounds on the transport process makes promising.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84087137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

[Effect of deltaran and melatonin on immune system in rats with experimental contact dermatitis]. 德尔他兰和褪黑素对实验性接触性皮炎大鼠免疫系统的影响。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.1615/INTJPHYSPATHOPHYS.V6.I3.10

O. Shandra

引用次数: 1

[Exocrine function of the pancreas in rats with experimental obesity]. [实验性肥胖大鼠胰腺的外分泌功能]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/FZ60.01.041

I. Leshchenko, V. Shevchuk, O. Savcheniuk, T. Falalieieva, S. Sukhodolia, T. Berehova

引用次数: 0

[Nitric oxide as a possible regulator of energy-dependent Ca2+ transport in mitochondria of uterine smooth muscle]. [一氧化氮作为子宫平滑肌线粒体中能量依赖性Ca2+运输的可能调节剂]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-01 DOI: 10.15407/FZ60.02.012

Iu V Danylovych, O. Kolomiiets, H. V. Danylovych, S. Kosterin

{"title":"[Nitric oxide as a possible regulator of energy-dependent Ca2+ transport in mitochondria of uterine smooth muscle].","authors":"Iu V Danylovych, O. Kolomiiets, H. V. Danylovych, S. Kosterin","doi":"10.15407/FZ60.02.012","DOIUrl":"https://doi.org/10.15407/FZ60.02.012","url":null,"abstract":"The influence of the donor and the precursor of NO, namely 100 mM sodium nitroprusside and sodium nitrite on the energo-dependent Ca(2+)-transport in isolated mitochondria from rat myometrium was investigated. Changes in the mitochondrial matrix Ca(2+)-concentration was evaluated by spectrofluorimetry using Ca2+ sensitive probe Fluo-4 AM. Mg(2+)-ATP-dependent Ca(2+)-accumulation on mitochondria in the presence of succinate significantly stimulated by nitric oxide, in particular, 100 microM sodium nitroprusside amplified the transport by 1.6 times relative to its control values. NO effect becomes significant only when the incubation of mitochondria with the compounds was performed. Ca(2+)-accumulation in the presence of sodium nitroprusside effectively suppressed by protonophore (CCCP) and ruthenium red (10 microM). It was concluded that inner mitochondrial membrane Ca(2+)-uniporter stimulated by nitrogen oxide. Ca(2+)-accumulation in mitochondria in the presence of sodium nitroprusside was not sensitive to the action of a specific permeability transition pore inhibitor cyclosporine (5 microM). This data indicates that the role of permeability transition pore is less significant than Ca(2+)-uniporter in the processes of Ca(2+)-transport in mitochondria under the nitric oxide action. Thus, nitric oxide stimulates the energo-dependent Ca(2+)-accumulation by myometrium mitochondria mediated their inner membrane Ca(2+)-uniporter functioning.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87197121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3