Experimental pathology最新文献

{"title":"USE OF INDICATORS OF THE STATE OF MICROCIRCULATION IN THE DIAGNOSTICS OF ARTERIAL HYPERTENSION IN SCHOOLCHILDREN","authors":"D. Nechytailo, T. Miheeva","doi":"10.24061/1727-4338.xviii.4.70.2019.9","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.9","url":null,"abstract":"Objective . Arterial hypertension is a disease characterized by a periodic or persistent increase of blood pressure that occurs on the background of excessive activity of the sympathoadrenal or renin-angiotensin-systems. The prevalence of hypertension in children is from 1% to 14%, among schoolchildren - 12-18%. The diagnosis of hypertension is established in children in the case when primary arterial hypertension persists for 1 year or more or earlier (in the presence of target organ damage). Purpose of the study. To evaluate and use indicators of the state of microcirculation in the diagnosis of arterial hypertension in schoolchildren. Materials and methods. 80 children of school age from the countryside and the city of Chernivtsi were examined. The average age of the children was 14.2 ± 0.11 years. The main group (40 persons) included children with high blood pressure, and the control group consisted of 40 clinically healthy children. Blood pressure was measured with an automatic blood pressure monitor with interchangeable cuffs, three times with an interval of 2 minutes. The evaluation of the state of peripheral microcirculation was carried out using computer capillaroscopy (quantitative and qualitative parameters were estimated). Results. In 26 children (65.0%) of the main group, the level of blood pressure was within the range of 90-95%, which is estimated as arterial prehypertension; in 14 children (35.0%) - exceeded the 95th percentile, which is regarded as grade 1 arterial hypertension. In all children of the control group, the level of blood pressure was within the range of 25-75 percentile corridor, which corresponds to the normal level of blood pressure. During biomicroscopy of the nail bed capillaries in the main group, 4 children (10%) showed a change in the shape of the capillaries, and there was a decrease in their number per unit area, in 9 children (22.5%) the phenomenon of \"sladge\" and slowing of blood flow with local spasm of capillaries was observed. In the control group, there were no such changes. When assessing the perivascular zone, linear density and capillary length, there were no differences in these indicators between the groups. A significant difference was observed in children of the main group among the following indicators:  average capillary width, distance between capillaries, average number of anastomoses, which is explained by the presence of local capillary spasm due to reduced oxygenation and slowed blood flow in children with arterial hypertension. Conclusions . In children with arterial hypertension, symptoms of microcirculation disturbance are more often manifested wiyh: a decrease in the width of the capillary, the presence of the phenomenon of \"sladge\", local spasm and a slowdown in blood flow, a change in the shape of the capillary, which can be explained by vegetative dysregulation, which in turn is accompanied by a violation of hemodynamics at all levels, including capillary.","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"36 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77683570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN INFLUENCE OF UNITHIOL ON THE FUNCTIONING OF KIDNEY","authors":"R. Kosuba, V. Gordienko, O. Perepelytsia","doi":"10.24061/1727-4338.xviii.4.70.2019.7","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.7","url":null,"abstract":"","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81405730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE ROLE OF THE NURSE IN PREVENTION OF IODODEFFICY DISEASES IN PERSONS OF DIFFERENT AGE GROUPS","authors":"T. Sorokman, M. Bachu, O. Makarova","doi":"10.24061/1727-4338.xviii.4.70.2019.14","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.14","url":null,"abstract":"","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89770963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE ANALYSIS OF CHANGES IN INDICATORS OF FIBRINOLYTIC ACTIVITY OF TISSUES OF INTERNAL ORGANS OF HYPOTHYROID RATS UNDER MELATONIN INFLUENCE","authors":"S. Anokhina","doi":"10.24061/1727-4338.xviii.4.70.2019.3","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.3","url":null,"abstract":"","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85906314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"USE OF PATHOPSYCHOLOGICAL DIAGNOSTICS IN A COMPLEX TREATMENT OF PATIENTS IN THE INSTITUTE OF CARDIOLOGY","authors":"Zh. H. Adaricheva","doi":"10.24061/1727-4338.xviii.4.70.2019.2","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.2","url":null,"abstract":"","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85695486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE COMPARATIVE CHARACTERISTICS OF THE NEUROENDOCRINE RESPONSES OF THE MAGNOCELLULAR AND PARVOCELLULAR VASOPRESSINERGIC NEURONS OF THE PARAVENTRICULAR NUCLEUS OF THE HYPOTHALAMUS UNDER THE INTERMITTENT HYPOXIC HYPOXIA","authors":"A. V. Abramov, V. A. Shamenko","doi":"10.24061/1727-4338.xviii.4.70.2019.1","DOIUrl":"https://doi.org/10.24061/1727-4338.xviii.4.70.2019.1","url":null,"abstract":"The brain is the central organ that forms the strategy of neuroendocrine adaptation to various stressors. One of the important mechanisms of brain neuroendocrine adaptation to stress is associated with the paraventricular nuclei of the hypothalamus (PVH). Neuropetides, which are synthesized in PVH, determine the reactivity of all parts of the hypothalamic-pituitary-adrenocortical axis, and ensure the adaptive reactions development and the formation of the body's resistance to stress. Objective.  To study the features of the functional state of vasopressinergic neurons of the paraventricular nucleus of the hypothalamus under influence of many days intermittent hypoxic hypoxia and in the long-term post-hypoxic period. Materials and methods.  The research was carried out on 30 male Wistar rats. Intermittent hypoxia was modeled by a daily 6-hour stay of rats at the stimulated altitude of 6000 m (pO 2  = 9.8%) for 15 days, the post-hypoxic period lasted 10 days. The expression of vasopressin, cFos, HIF-1  , and HIF-3   proteins in PVH subnuclei was investigated by quantitative immunofluorescence methods in serial frontal sections of the hypothalamus. Results.  Intermittent hypoxic hypoxia led to a significant increase in the content of the studied biomarkers of the functional activity of the peptidergic PVH neurons. So, in the lateral part of the posterior magnocellular subdivision of PVH, the vasopressin content was increased by 5.9 times, cFos protein by 80%, HIF-1   protein by 3.4 times and HIF- 3? protein by 3 times. After the end of the hypoxic effects, the vasopressin content in the region of the medial parvocellular subdivision of PVH was increased by 6.6 times, cFos protein - by 37%, HIF-1   protein by 2.5 times, and HIF-3   protein by 73%. In the posthypoxic period, a decrease in the content of vasopressin in PVH was noted. At the same time, while in the lateral part of the posterior magnocellular subdivision of PVH compared with the hypoxic period, the level of vasopressin was decreased by 4 times and remained by 43% higher than in the control animals, in the medial parvocellular subdivision of PVH the neuropeptide content decreased by 11 times and was by 38 % lower than before the onset of the hypoxic effects. Conclusions.  Hypoxic hypoxia stimulates an increase in the formation of vasopressin, proteins of the HIF family and cFos in the lateral part of the posterior magnocellular and medial parvocellular subdivisions of PVH. In the post-hypoxic period, inhibition of vasopressin synthesis in neurons of the medial parvocellular subdivision is observed while maintaining the increased functional activity of neurons in the lateral part of the posterior magnocellular subdivision of the PVH.","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76022284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Clinicopathological and Genetic Characteristics of High-grade Gliomas with Histone H3.3 G34 Mutation in Teenagers and Young adults","authors":"Yuanyuan Cheng","doi":"10.33696/PATHOLOGY.1.006","DOIUrl":"https://doi.org/10.33696/PATHOLOGY.1.006","url":null,"abstract":"33 Chromatin is composed of nucleosomes, with each nucleosome consisting of an octamer of two copies each of histones H3, H4, H2A, and H2B. Chromatin is critical for the control of transcription, replication, DNA repair and other aspects of genomic stability. In humans, there are different histone H3 variants. H3.3 proteins are expressed thoughout the cell cycle as well as in quiescent cells [1], while H3.1 and H3.2 are expressed only during the S-phase [2].","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"29 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83492823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Player in an Old Story: FBXO16 Prevents Breast Cancer Tumorigenesis through Disrupting Cellular Function of Nuclear ?-Catenin","authors":"Debasish Paul, S. Rapole, M. Santra","doi":"10.33696/pathology.1.004","DOIUrl":"https://doi.org/10.33696/pathology.1.004","url":null,"abstract":"22 β-Catenin is the central modulator of the canonical Wnt signaling pathway. Upon Wnt on state, β-Catenin is translocated to the nucleus and function as a transcription coactivator for several oncogenes. In Wnt off state, β-catenin is mostly localized in the cytoplasm and sequestered by the destruction complex, the negative regulator of β-catenin expression [2,3]. This destruction complex is composed of adenomatous polyposis coli (APC), casein kinase 1α (CK1α), glycogen synthase kinase 3α/β (GSK-3α/β), and AXIN1 [4-8]. CK1α initiates the degradation process of β-Catenin by phosphorylating it at Ser45, which is subsequently phosphorylated at Thr41, Ser37, and Ser33 by GSK3β [9]. The phosphorylated β-catenin is then polyubiquitinated by SCFβ-TrCP to promote its proteasomal degradation. In contrast, in the case of Wnt on state, β-catenin is released from the destruction complex and translocate to the nucleus. It is also reported that Norrin and R-Spondin activate Wnt signaling in stem cells and this activation is Wnt ligand independent [10-12]. They bind to LRP5/6 receptor and prevent the inactivation of LRP function by Rnf43/Znrf3. LRP5/6 functions as a coreceptor of Frizzled receptor and helps to separate Axin from the destruction complex. Thus, they help to translocate β-catenin in the nucleus through sequestration of Axin in the membrane. In the nucleus, β-catenin functions as a transcriptional coactivator to relate plethora of genes. Destruction complex-mediated sequestration of β-catenin is efficient to prevent cancer. However, it is ineffective in most of the cancers because of either mutation in the components of the destruction complex [13,14] or in the β-Catenin itself [15,16]. Among the components of destruction complex, loss of APC is main driver for constitutive translocation of β-catenin in colorectal cancer [17]. APC is mutated in almost 49% of colorectal cancers [18]. However, β-Catenin is predominantly mutated in hepatocellular carcinoma, endometrial cancer, and pancreatic cancer [19-21]. In addition to APC inactivation and β-catenin mutation, mutation in R-spondin/LRP5/ RNF43 plays crucial role in Wnt-dependent colorectal cancer. RNF43 is mutated in approximately 19% of colorectal cancer. Similarly, R-spondin is mutated in 10% of colorectal cancer. Interestingly, irrespective of the type of breast cancer, canonical Wnt signaling is activated in almost 50% of breast cancer and is closely associated with reduced overall survival of the breast cancer patients [22]. It is important to note that in most of the breast cancer patients, expression of Wnt ligands and receptors are elevated, whereas antagonists are silence.","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72855225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancytopenia: An Update","authors":"Subhashis Das","doi":"10.33696/pathology.1.005","DOIUrl":"https://doi.org/10.33696/pathology.1.005","url":null,"abstract":"Peripheral pancytopenia is not a disease by itself; rather it describes simultaneous presence of anemia, leucopenia and thrombocytopenia resulting from a number of disease processes. Detailed clinical history and meticulous physical examination along with baseline hematological investigations, provides invaluable information in the complete workup of pancytopenic patients, helping in systematic planning of further investigations to diagnose and ascertain the cause, avoiding a battery of unnecessary tests [1].","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75238932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Encapsulated Probiotics in Health Care","authors":"Esmat Alemzadeh, A. Oryan","doi":"10.33696/pathology.1.003","DOIUrl":"https://doi.org/10.33696/pathology.1.003","url":null,"abstract":"Esmat Alemzadeh1,2, Ahmad Oryan3* 1Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran 2Department of Medical Biotechnology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran 3Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran *Correspondence should be addressed to Ahmad Oryan; oryan@shirazu.ac.ir, oryan1215@gmail.com","PeriodicalId":12291,"journal":{"name":"Experimental pathology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74344594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}