FEMS immunology and medical microbiology最新文献_第4页

Secretion of matrix metalloproteinase-9 by macrophages, in vitro, in response to Helicobacter pylori 巨噬细胞分泌基质金属蛋白酶-9对幽门螺杆菌的体外反应

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.03.008

Bergin Philip James , Wen Sicheng , Pan-Hammarström Qiang , Quiding-Järbrink Marianne

引用次数: 20

Host defense effector molecules in mucosal secretions 粘膜分泌物中的宿主防御效应分子

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.03.004

G. Sandra Tjabringa , Joost B. Vos , Diana Olthuis , Dennis K. Ninaber , Klaus F. Rabe , Joost Schalkwijk , Pieter S. Hiemstra , Patrick L.J.M. Zeeuwen

引用次数: 54

CpG oligonucleotides partially inhibit growth of Mycobacterium tuberculosis, but not Salmonella or Listeria, in human monocyte-derived macrophages CpG寡核苷酸在人单核细胞来源的巨噬细胞中部分抑制结核分枝杆菌的生长，但不抑制沙门氏菌或李斯特菌的生长

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.05.007

Jennifer P. Wang, Tomoko Hayashi, Sandip K. Datta, Richard S. Kornbluth, Eyal Raz, Donald G. Guiney

{"title":"CpG oligonucleotides partially inhibit growth of Mycobacterium tuberculosis, but not Salmonella or Listeria, in human monocyte-derived macrophages","authors":"Jennifer P. Wang, Tomoko Hayashi, Sandip K. Datta, Richard S. Kornbluth, Eyal Raz, Donald G. Guiney","doi":"10.1016/j.femsim.2005.05.007","DOIUrl":"10.1016/j.femsim.2005.05.007","url":null,"abstract":"<div>Immunostimulatory DNA sequences and their synthetic oligonucleotide analogs (CpG-ODN) activate innate immunity and can stimulate antibacterial effects against numerous intracellular pathogens. While it has been shown previously that CpG-ODN inhibit growth of Mycobacterium avium in murine and human macrophages, we now report that Mycobacterium tuberculosis growth can be inhibited by CpG-ODN treatment of human monocyte-derived macrophages (hMDM). This inhibitory effect was reversed by IFN-γ, which has been shown repeatedly to enhance the growth of virulent M. tuberculosis in cultured hMDM. The antibacterial effect of CpG-ODN in human macrophages was specific for M. tuberculosis when compared to other intracellular pathogens including Listeria monocytogenes and Salmonella enterica serovar Dublin. These data indicate that CpG-ODN can improve the ability of hMDM to contain growth of virulent M. tuberculosis.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25174883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Expression and secretion of the protective antigen of Bacillus anthracis in Bacillus brevis 短芽孢杆菌中炭疽芽孢杆菌保护性抗原的表达与分泌

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.05.008

Gi-eun Rhie , Young-Mia Park , Jeong-hoon Chun, Cheon-Kwon Yoo, Won-Keun Seong, Hee-Bok Oh

{"title":"Expression and secretion of the protective antigen of Bacillus anthracis in Bacillus brevis","authors":"Gi-eun Rhie , Young-Mia Park , Jeong-hoon Chun, Cheon-Kwon Yoo, Won-Keun Seong, Hee-Bok Oh","doi":"10.1016/j.femsim.2005.05.008","DOIUrl":"10.1016/j.femsim.2005.05.008","url":null,"abstract":"<div>We used the Bacillus brevis-pNU212 system to develop a mass production system for the protective antigen (PA) of Bacillus anthracis. A moderately efficient expression-secretion system for PA was constructed by fusing the PA gene from B. anthracis with the B. brevis cell-wall protein signal-peptide encoding region of pNU212, and by introducing the recombinant plasmid, pNU212-mPA, into B. brevis 47-5Q. The clone producing PA secreted about 300μg of recombinant PA (rPA) per ml of 5PY-erythromycin medium after 4 days incubation at 30°C. The rPA was fractionated from the culture supernatant of B. brevis 47-5Q carrying pNU212-mPA using ammonium sulfate at 70% saturation followed by anion exchange chromatography on a Hitrap Q, a Hiload 16/60 Superdex 200 gel filtration column and a phenyl sepharose hydrophobic interaction column, yielding 70 <!-->mg rPA per liter of culture. The N-terminal sequence of the purified rPA was identical to that of native PA from B. anthracis. The purified rPA exhibited cytotoxicity towards J774A.1 cells when combined with lethal factor. The rPA formulated in either Rehydragel HPA or MPL-TDM-CWS adjuvant (Ribi-Trimix) elicited the expression of a large amount of anti-PA and neutralizing antibodies in guinea pigs and completely protected them against a 100 LD50 challenge with fully virulent B. anthracis spores.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25183036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Infection of epithelial and dendritic cells by Chlamydia trachomatis results in IL-18 and IL-12 production, leading to interferon-γ production by human natural killer cells 沙眼衣原体感染上皮细胞和树突状细胞导致IL-18和IL-12的产生，导致人类自然杀伤细胞产生干扰素-γ

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.02.010

Catherina Eliszbeth Hook, Malgosia K. Matyszak, John S. Hill Gaston

引用次数: 44

Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors 蛋白酶抑制剂作用下溶组织梭菌培养上清液对HeLa细胞的细胞毒作用的测定

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.03.005

Jarosław Jóźwiak , Aldona Komar , Ewa Jankowska , Gayane Martirosian

{"title":"Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors","authors":"Jarosław Jóźwiak , Aldona Komar , Ewa Jankowska , Gayane Martirosian","doi":"10.1016/j.femsim.2005.03.005","DOIUrl":"10.1016/j.femsim.2005.03.005","url":null,"abstract":"<div>Clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. This includes lethal toxin, clostripain and high-potassium-sensitive toxin. Since the number of C. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of C. histolyticum. In this study we evaluated in vitro the influence of four common protease inhibitors: aprotinin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin on the toxicity of C. histolyticum supernatant towards human epithelial HeLa cells. We show that aprotinin has no effect, while PMSF, TLCK and chymostatin potentiate the cytotoxic activity of C. histolyticum, probably by hindering natural defence mechanisms of cells. In addition, PMSF and TLCK block clostripain enzymatic activity, while chymostatin leaves it intact. Elevated cytotoxicity of the supernatant is not related to the quantity of high-potassium-sensitive toxin, as was reported previously, since desalted supernatant still exerted its strong toxic effect. Our results show that addition of protease inhibitors for treating diseases complicated by concurrent C. histolyticum infection must require special attention.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25218517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Cloning of mce1 locus of Mycobacterium leprae in Mycobacterium smegmatis mc2 155 SMR5 and evaluation of expression of mce1 genes in M. smegmatis and M. leprae 耻垢分枝杆菌mc2 155 SMR5中麻风分枝杆菌mce1基因位点的克隆及mce1基因在耻垢分枝杆菌和麻风分枝杆菌中的表达评价

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.05.004

Ramachandran Sarojini Santhosh, Shunmugiah Karutha Pandian , Nirmala Lini, Abdul Khader Shabaana , Avuthu Nagavardhini, Kuppamuthu Dharmalingam

{"title":"Cloning of mce1 locus of Mycobacterium leprae in Mycobacterium smegmatis mc2 155 SMR5 and evaluation of expression of mce1 genes in M. smegmatis and M. leprae","authors":"Ramachandran Sarojini Santhosh, Shunmugiah Karutha Pandian , Nirmala Lini, Abdul Khader Shabaana , Avuthu Nagavardhini, Kuppamuthu Dharmalingam","doi":"10.1016/j.femsim.2005.05.004","DOIUrl":"10.1016/j.femsim.2005.05.004","url":null,"abstract":"<div>Plasmid pSET152 is a broad host range mobilizable vector which integrates into streptomyces chromosome utilizing att site and int function of ∅C31. Transformation of this plasmid into Mycobacterium smegmatis mc2 155 SMR5 gave stable transformants carrying the pSET152 as an integrated copy. Integration occurred at the cross over sequence 5′TTG disrupting the gatA gene (Glu-tRNAGln amidotransferase subunitA), which is non-essential under conditions used. Recombinant pSET152 plasmids carrying mce1 locus of Mycobacterium leprae were used to construct M. smegmatis transformants carrying the mce1 locus in their chromosome. RT-PCR analysis revealed specific transcripts of M. leprae mce in M. smegmatis. The transcribed mRNA carried intergenic regions between genes of mce1 locus indicating that mce1 locus is an operon. Examination of M. leprae specific mRNA from lepromatous leprosy patient’s biopsy showed that mce locus is transcribed as an operon in the pathogen also.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25131051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Nontypeable Haemophilus influenzae-binding gangliosides of human respiratory (HEp-2) cells have a requisite lacto/neolacto core structure 人类呼吸细胞(HEp-2)的不可分型流感嗜血杆菌结合神经节苷具有必要的乳酸/新乳酸核心结构

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.03.007

Charles S. Berenson , Kelly B. Sayles , Jing Huang , Vernon N. Reinhold , Mary Alice Garlipp , Herbert C. Yohe

{"title":"Nontypeable Haemophilus influenzae-binding gangliosides of human respiratory (HEp-2) cells have a requisite lacto/neolacto core structure","authors":"Charles S. Berenson , Kelly B. Sayles , Jing Huang , Vernon N. Reinhold , Mary Alice Garlipp , Herbert C. Yohe","doi":"10.1016/j.femsim.2005.03.007","DOIUrl":"10.1016/j.femsim.2005.03.007","url":null,"abstract":"<div>Nontypeable Haemophilus influenzae (NTHI) are a major cause of human infections. We previously demonstrated high affinity and high specificity binding of NTHI to minor gangliosides of human respiratory (HEp-2) cells and macrophages, but not to brain gangliosides. We further identified the NTHI-binding ganglioside of human macrophages as α2,3-sialylosylparagloboside (IV3NeuAc-nLcOse4Cer, nLM1), which possesses a neolacto core structure that is absent in brain gangliosides. This supported a hypothesis that lacto/neolacto core carbohydrates are critical for NTHI-ganglioside binding. To investigate, we determined the core carbohydrate structure of NTHI-binding gangliosides of HEp-2 cells, through multiple approaches, including specific enzymatic degradation, mass spectral analysis and gas–liquid chromatography. Our analyses denote the following critical structural attributes of NTHI-binding gangliosides: (1) a conserved lacto/neolacto core structure; (2) requisite sialylation, which may be either internal or external, with α2,3 (human macrophages) or α2,6 (HEp-2 cells) anomeric linkages; (3) internalized galactose residues. Mass spectral and gas chromatographic analyses confirm that NTHI-binding gangliosides of HEp-2 cells possess lacto/neolacto carbohydrate cores and identify the structure of the major peak as NeuAcα2-6Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer (α2,6-sialosylparagloboside, nLM1). Collectively, our studies denote NTHI-binding gangliosides as lacto/neolacto series structures.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25218423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Determination of intracellular cytokines produced by Th1 and Th2 cells using flow cytometry in patients with brucellosis 用流式细胞术测定布鲁氏菌病患者Th1和Th2细胞产生的细胞内细胞因子

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.04.001

H. Handan Akbulut , S. Sirri Kilic , Vedat Bulut , Mehmet Ozden

{"title":"Determination of intracellular cytokines produced by Th1 and Th2 cells using flow cytometry in patients with brucellosis","authors":"H. Handan Akbulut , S. Sirri Kilic , Vedat Bulut , Mehmet Ozden","doi":"10.1016/j.femsim.2005.04.001","DOIUrl":"10.1016/j.femsim.2005.04.001","url":null,"abstract":"<div>The aim of the study was to evaluate intracellular interferon-γ (IFN-γ), and interleukin-4 (IL-4) levels in pre- and post-treatment periods of brucellosis patients and to determine the relationship between these parameters and patients’ clinical findings. Twenty-five patients diagnosed as brucellosis and 11 aged-matched healthy volunteers were included in the study. CD3+CD4+ T lymphocytes levels were significantly lower in patients with brucellosis as compared to the control group. CD3+CD8+ T lymphocytes and CD3+IFN-γ+ levels were increased in brucellosis patients compared with the control group. CD4+IFN-γ+ and CD4+IL-4+ levels were no different between patients and healthy individuals. CD3+IL-4+ levels decreased in patients compared with healthy controls. Pre-treatment CD3+IFN-γ+ levels dramatically increased in patients responsive to management compared with the unresponsive ones. In responsive cases, CD3+IFN-γ+ levels decreased statistically after the treatment while in unresponsive cases no meaningful change was observed with respect to treatment. Adding IFN-γ to the treatment for improving the depleted levels of IFN-γ can be beneficial in patients with brucellosis who shows a tendency to chronicity or patients who do not respond to the treatment.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25131572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Involvement of the virulence gene products of Yersinia enterocolitica in the immune response of infected mice 小肠结肠炎耶尔森菌毒力基因产物在感染小鼠免疫应答中的作用

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.05.010

Osamu Takeuchi , Tatsuo Suzuki , Ikuo Kawamura , Noritada Kobayashi , Asako Takizawa-Hashimoto , Masao Mitsuyama

{"title":"Involvement of the virulence gene products of Yersinia enterocolitica in the immune response of infected mice","authors":"Osamu Takeuchi , Tatsuo Suzuki , Ikuo Kawamura , Noritada Kobayashi , Asako Takizawa-Hashimoto , Masao Mitsuyama","doi":"10.1016/j.femsim.2005.05.010","DOIUrl":"10.1016/j.femsim.2005.05.010","url":null,"abstract":"<div>The virulence of Yersinia enterocolitica is known to be highly dependent on its virulence plasmid. However, it remains unclear whether the virulence plasmid is engaged also in the induction of cell-mediated immunity that is essential for protective immunity in the host. In this study, we have compared the induction of type 1 helper T cell immunity against Y. enterocolitica using a virulent strain (P+) harboring the pYV plasmid and an avirulent strain (P−) harboring no pYV. Spleen cells from both groups of mice immunized with 1/10 LD50 of P+ strain and those with 1/10 LD50 of P− strain produced a high level of gamma interferon (IFN-γ) upon stimulation with heat-killed bacteria, and CD4+ T cells were exclusively responsible for IFN-γ production. When crude Yersinia outer proteins (Yops) were used for antigenic stimulation, IFN-γ response of immune spleen cells against crude Yops was observed only in mice immunized with P+ strain. Flowcytometric analysis revealed a significant level of increase in IFN-γ-producing CD8+ T cells as well as the increase in IFN-γ-producing CD4+ T cells against crude Yops. These results suggest that the virulence plasmid of Y. enterocolitica is involved in the induction of Th1-type of possibly protective T cells in infected mice.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25191386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0