CpG oligonucleotides partially inhibit growth of Mycobacterium tuberculosis, but not Salmonella or Listeria, in human monocyte-derived macrophages

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI:10.1016/j.femsim.2005.05.007

Jennifer P. Wang, Tomoko Hayashi, Sandip K. Datta, Richard S. Kornbluth, Eyal Raz, Donald G. Guiney

{"title":"CpG oligonucleotides partially inhibit growth of Mycobacterium tuberculosis, but not Salmonella or Listeria, in human monocyte-derived macrophages","authors":"Jennifer P. Wang, Tomoko Hayashi, Sandip K. Datta, Richard S. Kornbluth, Eyal Raz, Donald G. Guiney","doi":"10.1016/j.femsim.2005.05.007","DOIUrl":null,"url":null,"abstract":"<div>Immunostimulatory DNA sequences and their synthetic oligonucleotide analogs (CpG-ODN) activate innate immunity and can stimulate antibacterial effects against numerous intracellular pathogens. While it has been shown previously that CpG-ODN inhibit growth of Mycobacterium avium in murine and human macrophages, we now report that Mycobacterium tuberculosis growth can be inhibited by CpG-ODN treatment of human monocyte-derived macrophages (hMDM). This inhibitory effect was reversed by IFN-γ, which has been shown repeatedly to enhance the growth of virulent M. tuberculosis in cultured hMDM. The antibacterial effect of CpG-ODN in human macrophages was specific for M. tuberculosis when compared to other intracellular pathogens including Listeria monocytogenes and Salmonella enterica serovar Dublin. These data indicate that CpG-ODN can improve the ability of hMDM to contain growth of virulent M. tuberculosis.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.007","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001264","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 15

Abstract

Immunostimulatory DNA sequences and their synthetic oligonucleotide analogs (CpG-ODN) activate innate immunity and can stimulate antibacterial effects against numerous intracellular pathogens. While it has been shown previously that CpG-ODN inhibit growth of Mycobacterium avium in murine and human macrophages, we now report that Mycobacterium tuberculosis growth can be inhibited by CpG-ODN treatment of human monocyte-derived macrophages (hMDM). This inhibitory effect was reversed by IFN-γ, which has been shown repeatedly to enhance the growth of virulent M. tuberculosis in cultured hMDM. The antibacterial effect of CpG-ODN in human macrophages was specific for M. tuberculosis when compared to other intracellular pathogens including Listeria monocytogenes and Salmonella enterica serovar Dublin. These data indicate that CpG-ODN can improve the ability of hMDM to contain growth of virulent M. tuberculosis.

查看原文本刊更多论文

CpG寡核苷酸在人单核细胞来源的巨噬细胞中部分抑制结核分枝杆菌的生长，但不抑制沙门氏菌或李斯特菌的生长

免疫刺激DNA序列及其合成的寡核苷酸类似物(CpG-ODN)可激活先天免疫，并可刺激对许多细胞内病原体的抗菌作用。虽然之前已经有研究表明CpG-ODN可以抑制鸟分枝杆菌在小鼠和人巨噬细胞中的生长，但我们现在报道CpG-ODN治疗人单核细胞源性巨噬细胞(hMDM)可以抑制结核分枝杆菌的生长。这种抑制作用被IFN-γ逆转，IFN-γ多次被证明可以促进培养的hMDM中毒力结核分枝杆菌的生长。CpG-ODN在人巨噬细胞中的抑菌作用与单核增生李斯特菌和都柏林沙门氏菌等其他细胞内病原体相比，对结核分枝杆菌具有特异性。这些数据表明，CpG-ODN可以提高hMDM抑制致病性结核分枝杆菌生长的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

FEMS immunology and medical microbiology 医学-传染病学

自引率

0.00%

发文量

审稿时长

3-8 weeks