Folia Pharmacologica Japonica最新文献_第3页

[Revolutionizing physiology: advances in organ-on-a-chip technology]. [革命性的生理学：器官芯片技术的进展]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.26019

Qiang Li, Ken Takahashi

{"title":"[Revolutionizing physiology: advances in organ-on-a-chip technology].","authors":"Qiang Li, Ken Takahashi","doi":"10.1254/fpj.26019","DOIUrl":"10.1254/fpj.26019","url":null,"abstract":"To address the low predictability (92% clinical failure rate) and high costs associated with species differences in traditional animal testing, this research advocates for New Approach Methodologies (NAMs) in alignment with the US FDA Modernization Act 2.0. The central achievement presented is the development of a \"human Heart-on-a-chip.\" This microfluidic device co-cultures human iPS-derived cardiomyocytes, fibroblasts, and vascular endothelial cells to mimic living 3D tissue structures and fluid environments. The system demonstrated superior physiological reproducibility compared to conventional methods in evaluating drug responses (e.g., nifedipine) and detecting fatal side effects like QT prolongation. In addition to the heart, applications are advancing across diverse organ and disease models, including the construction of ischemia-reperfusion injury models using kidney chips and pulmonary fibrosis models using lung chips, the evaluation of barrier functions via blood-brain barrier (BBB) chips, and the analysis of interactions between cancer cells and immune cells using cancer chips. It is concluded that these technologies enable high-precision in vitro reproduction of human physiology and pathology, thereby improving the efficiency of drug screening, reducing economic losses, and accelerating the paradigm shift toward the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation.","PeriodicalId":12208,"journal":{"name":"Folia Pharmacologica Japonica","volume":"161 5","pages":"356-362"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Role of mechanosensitive ion channels in skeletal muscle regeneration]. [机械敏感离子通道在骨骼肌再生中的作用]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25077

Kotaro Hirano, Yuji Hara

引用次数: 0

[A social cognitive map in the hippocampus]. [海马体中的社会认知地图]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25109

Kisa Watanabe, Kano Izumo, Sena Iijima, Yuji Ikegaya, Nobuyoshi Matsumoto

引用次数: 0

[Gut microbiota-mediated mechanisms of Kampo medicine in cancer supportive care and their impact on future treatment outcomes]. [肠道微生物群介导的康坡药在癌症支持治疗中的机制及其对未来治疗结果的影响]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.26048

Kanako Miyano

引用次数: 0

[Preface]. (前言)。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.26054

Schuichi Koizumi, Misa Arizono

引用次数: 0

[The role of the periaqueductal gray in behavioral selection under threat]. [输水管周围灰质在威胁下的行为选择中的作用]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25086

Natsuko Hitora-Imamura, Yuki Honshuku

{"title":"[The role of the periaqueductal gray in behavioral selection under threat].","authors":"Natsuko Hitora-Imamura, Yuki Honshuku","doi":"10.1254/fpj.25086","DOIUrl":"10.1254/fpj.25086","url":null,"abstract":"Fear responses and defensive behaviors are essential for survival, allowing animals to detect threatening stimuli and classify them as aversive. Neural circuits governing fear dynamically select adaptive defensive strategies, such as freezing or flight, depending on threat imminence and escape availability. The lateral/ventrolateral periaqueductal gray (l/vlPAG) plays a central role in these behaviors, and activation of its excitatory neurons elicits diverse fear responses based on subregion, projection target, and stimulation intensity. A subset of l/vlPAG neurons projects to the magnocellular nucleus of the medulla (Mc), yet the contribution of this pathway to intensity-dependent defensive behaviors and negative emotional valence remained unclear. Using in vivo calcium imaging, Mc-projecting l/vlPAG neurons were shown to be strongly activated by aversive stimuli. Optogenetic stimulation at two intensities revealed that strong activation induced flight behavior and place aversion, whereas weak activation evoked freezing. These results indicate that strong activation of the l/vlPAG-Mc pathway drives active defensive behaviors coupled with negative valence, while weak activation mediates passive defensive responses. Overall, these findings suggest that adaptive switching between defensive strategies is achieved through modulation of activation levels within l/vlPAG output pathways, allowing rapid responses to varying threat levels and environmental context. The l/vlPAG functions as a hub integrating diverse inputs and selectively recruiting neuronal subpopulations, and dysfunction in this circuit may contribute to fear- and anxiety-related psychiatric disorders. Understanding the l/vlPAG and its input-output pathways is therefore critical for identifying potential therapeutic targets.","PeriodicalId":12208,"journal":{"name":"Folia Pharmacologica Japonica","volume":"161 2","pages":"96-100"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147325209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25065

Hideaki Tagashira

引用次数: 0

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25092

Akihiko Tanimura

引用次数: 0

[Discovery of heat-induced calcium release via ryanodine receptors and a novel mechanism of thermal runaway]. [热诱导钙通过ryanodine受体释放和热失控新机制的发现]。

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25035

Madoka Suzuki, Kotaro Oyama, Toshiko Yamazawa

{"title":"[Discovery of heat-induced calcium release via ryanodine receptors and a novel mechanism of thermal runaway].","authors":"Madoka Suzuki, Kotaro Oyama, Toshiko Yamazawa","doi":"10.1254/fpj.25035","DOIUrl":"https://doi.org/10.1254/fpj.25035","url":null,"abstract":"Regulation of thermogenesis in mammals is essential for maintaining body temperature homeostasis under fluctuating environmental temperature. Impairments in this regulation can lead to severe conditions, including fever or heatstroke. This review focuses on malignant hyperthermia (MH), a pathological escalation of thermogenesis in skeletal muscle. It highlights the role of type 1 ryanodine receptor (RYR1), a Ca2+ release channel, based on our recent studies. Previous studies have revealed that genetic mutations in RYR1 are associated with muscle disorders including MH, which are characterized by abnormal Ca2+-induced Ca2+ release (CICR). To test our hypothesis that RYR1 channel function is closely related to thermogenesis, we examined cultured cell lines expressing wild-type or MH-related mutants of RYR1, as well as muscle cells prepared from MH model mice. Using a local heating microscopy combined with fluorescence temperature imaging, we identified a novel phenomenon termed heat-induced Ca2+ release (HICR). Furthermore, our results indicate that anesthesia induces simultaneous increases in temperature and cytoplasmic Ca2+ concentration in muscle cells. Based on these findings, we propose a positive feedback loop where HICR drives further Ca2+ release during MH episodes, causing thermogenesis and further elevation of body temperature. This review summarizes our experimental results that were presented at the symposium, providing greater detail on the mechanisms underlying MH pathogenesis and the role of RYR1 in thermal regulation.","PeriodicalId":12208,"journal":{"name":"Folia Pharmacologica Japonica","volume":"161 1","pages":"33-37"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of fluorescence lifetime imaging-based biosensors for quantitative visualization]. 基于荧光寿命成像的定量可视化生物传感器的发展

Folia Pharmacologica Japonica Pub Date : 2026-01-01 DOI: 10.1254/fpj.25044

Satoshi Arai

{"title":"[Development of fluorescence lifetime imaging-based biosensors for quantitative visualization].","authors":"Satoshi Arai","doi":"10.1254/fpj.25044","DOIUrl":"10.1254/fpj.25044","url":null,"abstract":"Fluorescent biosensors have become essential tools in life sciences, enabling the visualization of the spatiotemporal dynamics of signaling molecules at the cellular level. In particular, intensity-based sensors-where changes in the concentrations of signaling molecules are detected as changes in fluorescence intensity-are widely used due to their versatility. However, such sensors are often affected by several factors, including variations in biosensor concentration, photobleaching, optical path settings, and focus drift, which hamper quantitative analysis. To overcome these challenges, we have been developing fluorescence lifetime imaging microscopy (FLIM)-based biosensors that utilize fluorescence lifetime-a parameter independent of probe concentration and imaging conditions-as a robust and reliable readout. Our research has focused on the quantitative visualization of physiological parameters, particularly those relevant to skeletal muscle homeostasis and ion channel activity. One example is a small-molecule fluorescent temperature sensor designed to quantify temperature changes in subcellular compartments. This sensor, based on an organic dye, enables targeting to organelle membranes and provides high spatial resolution, allowing precise detection of local heat production, such as that occurring in the mitochondria of brown adipocytes. In parallel, we have developed genetically encoded fluorescent protein-based sensors that correlate fluorescence lifetime values with the concentrations of signaling molecules such as ATP. These sensors have enabled the quantitative imaging of ATP dynamics in various cell types and multicellular systems. Furthermore, we are constructing a flexible sensor development platform, paving the way for the creation of diverse biosensors that can contribute to comprehensive studies in muscle physiology.","PeriodicalId":12208,"journal":{"name":"Folia Pharmacologica Japonica","volume":"161 1","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0