{"title":"What happened to the genus Gammarus (Crustacea: Amphipoda) in Central Asia","authors":"Boris Sket, D. Sidorov, Z. Hou","doi":"10.14720/abs.62.2.15754","DOIUrl":"https://doi.org/10.14720/abs.62.2.15754","url":null,"abstract":"We examined the phylogenetic relationships in the unusually structured fauna of the amphipod genus Gammarus in Central Asia, trying to explain its causes. Mitochondrial DNA sequence data were used in MP and ML and Bayesian analyses to generate a phylogenetic hypothesis. All of the recently collected Gammarus spp. specimens belong to the G. lacustris aggregate, except scarce marginal populations of G. balcanicus and G. komareki aggregates. Although molecularly closely related, these. G. lacustris agg. populations are morphologically and ecologically equally diverse as are the numerous European Gammarus spp. Only few populations are morphologically close to the morphotype of G. lacustris. There is a more than 2,000 km wide gap between the eastern and western populations-species of G. balcanicus agg. Since Gammarus lacustris can be spread by birds, and its Asian relatives are molecularly significantly diverse, we suppose that it invaded Central Asia several times and from several sources. It most probably differentiated to diverse morphotypes after reaching Asia. The area of the cryophilic and less nomadic G. balcanicus could have been reached and conquered only by waterways. Its area was probably continuous in the past and divided by aridification during the Neogene. The strong historical aridifications probably extirpated the probably higher Gammarus diversity in Central Asia, including G. balcanicus. This opened the area and its habitats for the reiterated invasions of the particularly euryoecious G. lacustris s.l., followed by its morphological and ecological diversification.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121921597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irina L. Maslennikova, Ekaterina G. Orlova, Julia S. Gizatullina, M. Starčič Erjavec, Natalia P. Loginova, Yana N. Troinich, Marina V. Kuznetsova
{"title":"Effect of per oral administration of the ŽP strain, a new potential probiotic, on rats","authors":"Irina L. Maslennikova, Ekaterina G. Orlova, Julia S. Gizatullina, M. Starčič Erjavec, Natalia P. Loginova, Yana N. Troinich, Marina V. Kuznetsova","doi":"10.14720/abs.62.2.15750","DOIUrl":"https://doi.org/10.14720/abs.62.2.15750","url":null,"abstract":"Bacterial antimicrobial resistance is worldwide rising and is already reaching worrisome levels, hence WHO released the priority bacterial pathogen list for research and development of new antimicrobial agents. ŽP strain, a new Nissle 1917 based antimicrobial strain harbors a bacteriocin, colicin ColE7, bacterial conjugationbased “kill” – “anti-kill” antimicrobial system. In previous studies the efficiency of ColE7 bacterial conjugation-based antimicrobial system was shown against different Escherichia coli strains. In this study its effect after the per oral administration of the ŽP strain, on the host, namely on rats of both sexes was studied. The following parameters were analyzed weight, histology of intestine, spleen and Peyer’s patches, blood biochemistry, hematology and microbicide activity of leukocytes and peritoneal macrophages. Our results showed that when administered at a dose of 5 × 108 CFU in the drinking water the ŽP strain is safe and responsible for control and maintenance of an adequate local and non-specific immune response and can also improve the performance of animals in terms of weight gain. Hence our results indicate that it is a promising probiotic.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126142323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fitness evaluation of Oenopia conglobata contaminata (Menetries) (Col.: Coccinellidae) fed on different diets","authors":"F. Hassani, A. Shirvani, M. Rashki","doi":"10.14720/abs.62.2.15741","DOIUrl":"https://doi.org/10.14720/abs.62.2.15741","url":null,"abstract":"Due to the importance of coccinellid predators as biological control agents of insect pests, the effects of different factitious and artificial diets on reproductive parameters of Oenopia conglobata contaminata (Menetries) were studied under laboratory conditions (26±1ºC, 60±5% relative air humidity and a photoperiod of 16h light : 8 h dark). In this study, various combinations of Ephestia kuehniella (Zeller) eggs, bee pollen, cysts of Artemia urmiana Günther and lyophilized artificial diet were used. All diets affected fitness parameters of the ladybeetle. The adult weight (12.00±1.30 mg), fecundity (725±29.31 eggs), oviposition rate (15.72±0.85 eggs), fertility (4.86±0.34 larvae) and number of egg clusters (66.16±2.87) were higher on unlimited E. kuehniella eggs plus pollen (UEkP) than those on the other diets. The adults lived longer when fed on EkPLd (the E. kuehniella eggs plus pollen and a mixture of lyophilized diet) and UEkP diets (58.57±2.38 and 62.77±3.64 days, respectively) than when fed on the other offered diets. More eggs were hatched when adults reared on EkPLd and EkLd (the E. kuehniella eggs plus a mixture of lyophilized diet) diets(36.44±1.93 and 40.01±2.58 %, respectively). The parameter values were increased when the E. kuehniella eggs were used in a mix with bee pollen and artificial lyophilized diet and resulted in producing the heavier adults with longer longevity and more fertile eggs. Certainly, the diet will be more cost-effective than natural prey for mass production of the predatory coccinellid, O. conglobata contaminata.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130497547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of the toxicity, antioxidant and antimicrobial activities of some cyanobacterial strains isolated from different habitats","authors":"Nooshin Rajabpour, B. Nowruzi, Maryam Ghobeh","doi":"10.14720/abs.62.2.15753","DOIUrl":"https://doi.org/10.14720/abs.62.2.15753","url":null,"abstract":"Cyanobacteria are known as a source of fine chemicals, renewable fuels, and toxic compounds. The present study aimed at evaluating the toxicity and antioxidant and antimicrobial activities of four cyanobacterial strains isolated from different habitats. Due to the lack of information regarding the relationship between toxicity and biological activity of the cyanobacteria in terrestrial and aquatic ecosystems of Iran, we decided to conduct a preliminary study on the cyanobacterial strains in order to identify the potentially toxic cyanobacteria strains. In this respect, biosynthesis genes related to cyanobacterial toxins, anatoxins (anaC gene), nodularins (ndaF gene) and microcystins (mcyG gene) were amplified. In addition, antioxidant, antimicrobial and biochemical properties of cyanobacterial strains have also been evaluated. The results of the molecular analysis demonstrated that only Fischerella sp. contained the microcystins (mcyG) gene. In fact, this strain encounters numerous predators in its habitat, therefore antibacterial and antioxidant metabolites found in this strain have thought to play an important role in defense mechanisms. This case is the documentation of toxicity and promotion of biological activities of a soil cyanobacterium regarding survival in competitive ecological niches.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116467738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Allelopathic effect of aqueous extracts of Canadian goldenrod on germination and growth of radish","authors":"Alvina Leticia Anžlovar, S. Anžlovar","doi":"10.14720/abs.62.2.15740","DOIUrl":"https://doi.org/10.14720/abs.62.2.15740","url":null,"abstract":"In this study we tested the effects of Solidago canadensis extracts on seed germination and early growth of radish (Raphanus sativus). The aqueous extracts of 2.5% (m/v) concentration were prepared from roots, rhizomes, stems, leaves and inflorescences and applied onto the filter paper in Petri dishes where radish seeds were sown. We determined the germination rate and seedling growth for four days. The extracts from leaves and inflorescences delayed germination, whereas extracts from stems and roots had no significant effect on seed germination. The extracts from rhizome had a slightly stimulatory effect on the seed germination and promoted shoot length of radish seedlings, while extracts from leaves inhibited root and shoot length and seedlings development.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128053605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Accessibility of school health education is an underlying factor for differences in knowledge about sexually transmitted infections of secondary school pupils","authors":"P. Golja, Kaja Antončič","doi":"10.14720/abs.62.1.15738","DOIUrl":"https://doi.org/10.14720/abs.62.1.15738","url":null,"abstract":"Health education constituted an individual subject in secondary schools in Slovenia before 1985. Thereafter it was disintegrated and its topics incorporated into different school subjects. Health education topics are required in the extent of 15 hours in upper secondary general education schools, but in the extent of 0 to 18 hours in other secondary school programmes. The present study compared the knowledge about sexually transmitted infections of pupils of two 4-year secondary school programmes with different health education requirements. The results demonstrated that different accessibility of school health education results in distinct differences in knowledge about the existence, sources of infection, symptoms, and protection against sexually transmitted infections between pupils of different secondary school programmes. Despite the general conception of overloaded curriculums, more than two thirds of pupils of both educational programmes expressed the need of receiving more information in sexual education topics, with systematic medical exam selected as the most suitable form of sexual education, closely followed by Biology class, and school project day. The results call for the attention of educational policy makers, as they clearly emphasise the need of establishing systematic and comprehensive school health education for all pupils, not only for those enrolled in the selected secondary school programmes.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127827878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Edulamudi, Anthony Johnson Anthony Masilamani, Venkata Ramana Sai Gopal Divi, Vishnuvardhan Zakkula, Umamaheswara Rao Vanga, Veera Mallaiah Konada
{"title":"Symbiotic efficiency, biosorption and the growth of rhizobia on Horse gram plants under aluminium stress","authors":"P. Edulamudi, Anthony Johnson Anthony Masilamani, Venkata Ramana Sai Gopal Divi, Vishnuvardhan Zakkula, Umamaheswara Rao Vanga, Veera Mallaiah Konada","doi":"10.14720/abs.62.1.15737","DOIUrl":"https://doi.org/10.14720/abs.62.1.15737","url":null,"abstract":"The aim of the present study was to evaluate the tolerance potential of Horse gram rhizobia to aluminium (Al) toxicity, the enhancement in pod formation, symbiotic efficiency and biosorption potential in the rhizobia inoculated Horse gram (Macrotyloma uniflorum (Lam.) Verdc.) plants. Initially, 32 isolates of Horse gram rhizobia were screened for their tolerance of Al in growth media. Among the 32 strains, HGR 4, 6, 13 and 25 that were more tolerant were inoculated individually to Horse gram plants and the plants were then screened for the ability of pod formation, symbiotic efficiency and biosorption potential. Among them, maximum pod formation was observed in Horse gram upon inoculation with HGR-6 and grown at 400 µg g-1 of Al. Maximum nodulation was observed in Horse gram upon inoculation with HGR-6 and HGR-13 grown at 200 µg g-1 Al. Leghaemoglobin content was maximumon inoculation with HGR-13 at 400 µg g-1 of Al. The strain HGR-13 has shown biosorption potential in soil and as well as in root nodules even at 300 µg g-1 of Al though it was maximum at 100 µg g-1. This study demonstrated that the Horse gram plantsinoculated with Rhizobium strains HGR - 4, 6, 13 and 25, besides having nitrogen fixing ability also have the ability to grow in Al contaminated soils. Hence, Horse gram plants associated with these strains of rhizobia could be used in phytoremediation ofmetal (Al) contaminated soils.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123815295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mnogotere vloge silicija izboljšajo uspevanje rastlin","authors":"Mateja Grašič","doi":"10.14720/abs.62.1.15735","DOIUrl":"https://doi.org/10.14720/abs.62.1.15735","url":null,"abstract":"Prispevek na podlagi širokega pregleda literature obravnava vlogo silicija pri rastlinah, od same pojavnosti silicija v tleh preko mehanizmov privzema in prenosa, do nalaganja in deleža silicija v rastlinah. Članek nadalje zajema evolucijski vidik pojavnosti silicija pri rastlinah ter izpostavlja njegove ključne vloge pri uspevanju rastlin in blaženju negativnih učinkov številnih stresnih dejavnikov ter njegovo uporabnost v kmetijstvu.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"259 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115809913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Golob, Ana Kugovnik, I. Kreft, A. Gaberščik, M. Germ
{"title":"2 The interactions between UV radiation, drought and selenium in different buckwheat species","authors":"A. Golob, Ana Kugovnik, I. Kreft, A. Gaberščik, M. Germ","doi":"10.14720/abs.62.1.15736","DOIUrl":"https://doi.org/10.14720/abs.62.1.15736","url":null,"abstract":"Here we summarise the outcomes on biochemical response and growth of buckwheat with respect to UV radiation, drought and selenium, as well as their interactions. Buckwheats are taxonomically and morphologically very diverse. They have been subjected to more or less intensive breeding, which results in higher susceptibility to environmental constraints including drought and also in different potential to cope and exploit UV radiation. The responses of different buckwheats to UV, drought and their combination differed in different species. Selenium treatment may mitigate negative effects of environmental constraints on buckwheat. Regarding the production of UV absorbing compounds, buckwheats mainly positively respond to UV dose. Ca druses in buckwheat seem to be important in affecting UV penetration.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124187419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"‘Only introduced’ or ‘invasive’: spread of the alga Aulacoseira ambigua f. japonica from Asia to Africa and Europe","authors":"T. Eleršek","doi":"10.14720/abs.61.2.15893","DOIUrl":"https://doi.org/10.14720/abs.61.2.15893","url":null,"abstract":"\u0000 \u0000 \u0000Many non-native algal species are found in waters all over the world. Many terms are used to describe such organisms that have expanded their distribution. However, a unified model or concept remains to be defined, as how biological ‘invasions’ are seen depends on the perspective. Understanding invasive organisms is important for biodiversity, science policy and water management. During monitoring sampling at Slivniško Lake (Slovenia) in 2016, the curved diatom Aulacoseira ambigua f. japonica Tuji & D.M. Williams was identified. This species originates from Japan, from where it was described more than 100 years ago. The chronology of the published distributions of A. ambigua f. japonica defines its spread from Japan to Asia, then to western Russia and South Africa, and now to central Europe. This study provides further evidence that A. ambigua f. japonica has become established in Europe (Slovenia), as supported by light microscopy and scanning electron microscopy. To the best of our knowledge, this is the first qualitative and quantitative description of A. ambigua f. japonica in Europe. It has been suggested that the shape of these algal colonies is significant for the interpretation of ecological information, and indeed, also in the present case, spiral colonies were found in this eutrophic water body. Although curved cells and colonies can also be interpreted as (sub)populations or morphological variants, only detailed molecular studies can reveal if these also have taxonomic significance. \u0000 \u0000 \u0000","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121439823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}