{"title":"Distribution of epilithic diatoms in the Savinja River flowing through an urban landscap","authors":"Igor Železnik, Doroteja Čatorič, Mihael J. Toman","doi":"10.14720/abs.59.2.15870","DOIUrl":"https://doi.org/10.14720/abs.59.2.15870","url":null,"abstract":"The catchment area in urban and agricultural landscapes is greatly influenced by human activities that reflect also in physical and chemical characteristics of water as well as in species diversity in water bodies. The diversity and the species composition of epilithic diatom communities in the Savinja River, as well as basic environmental parameters were analysed. Sampling sites were selected in reaches subjected to different influences from the catchment area and with different physical and chemical characteristics. Samples were collected at the site where the Savinja River enters the urban area of the town Celje, at the end of urban landscape and downstream of the Celje waste water treatment plant outflow. The most common and dominant diatom species in the periphyton community was Achnanthes biasolettiana. Other common diatom taxa that were found in all samples and at least in one sample exceeded relative abundance of 10% were Nitzschia fonticola, Amphora pediculus and Nitzschia dissipata. The results of the redundancy analyses (RDA) revealed that the variance of the epilithic diatom community was explained by O2 saturation(35%) and saprobic index (33% of TVE). Diatom species richness was positively correlated with O2 saturation. Shannon-Wienerdiversity index was positively correlated with saprobic index values based on all algae and trophic index calculated on the base of diatoms indicating a relatively low organic matter and nutrient input into the river system. The results showed that no significant changes in epilithic diatom species composition and no negative impacts on diversity of epilithic diatom community in the Savinja River were detected on its flow through the urban landscape. Moreover, changes between the seasons were more evident than changes between sampling sites, confirming the importance of sampling date for monitoring.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133527302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fluorescent markers in microscopy: photophysical characteristics and applications in cell biology","authors":"Urban Bogataj, J. Štrus, N. Žnidaršič, M. Kreft","doi":"10.14720/abs.59.2.15855","DOIUrl":"https://doi.org/10.14720/abs.59.2.15855","url":null,"abstract":"In the fluorescence microscopy of biological specimens the structures in cells and tissues usually need to be labelled with various fluorescent markers. The three main groups of fluorescent markers are small organic fluorochromes, fluorescent proteins and quantum dots. Fluorescent markers differ according to photophysical properties and binding specificity for the selected target structures in the sample. For the labelling of specific structures with small organic fluorochromes or quantum dots it is usually necessary to conjugate them with target-specific macromolecules. For the labelling with fluorescent proteins it is necessary to introduce a fluorescent protein gene into the observed cells or organism. The most important photophysical properties of fluorescent markers are absorption and emission spectra, Stokes shift, extinction coefficient and quantum yield. Currently, various super-resolution fluorescent microscopy techniques exploit special fluorochromes that enable fluorescence modulation by specific wavelength illumination, to achieve the resolution below the diffraction limit.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124601988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Germination rate of stinkwort (Dittrichia graveolens) and false yellowhead (D. viscosa) in relation to salinity","authors":"M. Grašič, S. Anžlovar, S. Strgulc Krajšek","doi":"10.14720/abs.59.1.15694","DOIUrl":"https://doi.org/10.14720/abs.59.1.15694","url":null,"abstract":"This study was conducted to investigate the effect of salinity on germination rate of stinkwort (Dittrichia graveolens (L.) Greuter) and false yellowhead (D. viscosa (L.) Greuter). Lettuce (Lactuca sativa L.) was used as a positive control. Seeds of all three test species were sown on agar plates with three different NaCl treatments (2.5 g NaCl/L ≈ 42 mM NaCl, 5 g NaCl/L ≈ 85 mM NaCl and 10 g NaCl/L ≈ 171 mM NaCl) and control treatment without NaCl. The three tested species germinated under all salinity conditions. However, they thrived best under control conditions and their germination rate was gradually declining with increasing salinity. Additionally, the start of their germination was delayed with increasing NaCl concentration. According to our findings, we can conclude that both Dittrichia species are very tolerant to salinity.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126992740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of leaf extracts of crack willow (Salix fragilis) on the growth of Japanese knotweed (Fallopia japonica)","authors":"J. Dolenc Koce","doi":"10.14720/abs.59.1.15695","DOIUrl":"https://doi.org/10.14720/abs.59.1.15695","url":null,"abstract":"Japanese knotweed (Fallopia japonica) is one of the most invasive of species in Europe, and can substantially reduce local native biodiversity. In the present study, the allelopathic potential of crack willow (Salix fragilis) on growth of Japanese knotweed was investigated.Aqueous extracts of 0.1% and 1% (w/v) were prepared from liophilised willow leaves and used for watering of young knotweed plants. Their growth was monitored for 196 days. Shoot height and leaf number were not affected but the mass of leaves and especially roots was reduced (up to 32%). At the end of experiment, biochemical characteristics related to physiological state (photochemical efficiency of PSII, protein content, enzyme activity of guaiacol peroxidase, lipid peroxidation) were measured. Mostly, they were at control levels, but the activity of guaiacol peroxidase and lipid peroxidation in roots increased. The extracts of crack willow showed moderate inhibitory effect on roots of treated knotweeds while the growth of shoots was unaffected. Given the root reduction described here, further studies with willow extracts and field studies with crack willow and Japanese knotweed plants would be reasonable.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129964335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Control of alpine dock (Rumex alpinus) by non-chemical methods","authors":"U. Šilc, M. Gregori","doi":"10.14720/abs.59.1.15696","DOIUrl":"https://doi.org/10.14720/abs.59.1.15696","url":null,"abstract":"Alpine dock (Rumex alpinus) is a troublesome weed particularly in protected zones or Natura 2000 areas, in which only non-chemical control measures can be applied. The aim of our study was to investigate by means of a field experiment the effectiveness of various non-chemical methods: mowing, manual removal, heating, foil and grazing by cattle and pigs. Floristic changes, cover, number of shoots and biomass were monitored at 14-day intervals for three consecutive years. Manual removal and foil were most successful, with almost complete removal of the biomass and cover of alpine dock, and mowing, which reduced the cover to 50%. Other methods were not as efficient. Animals avoid grazing on R. alpinus and heat merely supresses dock growth for a short period. ","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124366928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pavlína Škarkováa, Tea Romih, M. Kos, Sara Novak, Veno Kononenko, A. Jemec, M. Vávrová, D. Drobne
{"title":"Gold nanoparticles do not induce adverse effects on terrestrial isopods Porcellio scaber after 14-day exposure","authors":"Pavlína Škarkováa, Tea Romih, M. Kos, Sara Novak, Veno Kononenko, A. Jemec, M. Vávrová, D. Drobne","doi":"10.14720/abs.59.1.15697","DOIUrl":"https://doi.org/10.14720/abs.59.1.15697","url":null,"abstract":"Despite the anticipated environmental release of anthropogenic gold nanoparticles (AuNPs), there is currently not enough data on their potential impact on terrestrial environment. In the current study, we investigated the effects of considerably low concentrations of AuNPs on terrestrial isopods (Porcellio scaber) after 14 days of exposure. The effects on mortality, weight change, feeding rate, avoidance/preference feeding behavior, and cell membrane destabilization of digestive gland cells were followed. In parallel, the accumulation of Au in the digestive glands was measured. Our results show that none of the tested parameters was affected in isopods under given exposure doses (10 and 60 µg Au/g dry leaf) and exposure duration. No Au was assimilated in the digestive glands. Also, the same doses of the reference chemical, AuCl3, showed no effect. We conclude that these concentrations of AuNPs are safe for terrestrial isopods. We encourage reporting the results showing no adverse effects of nanoparticles to balance the prevailing publication of their adverse effects. This will help to build a realistic public perception of the environmental risk of nanomaterials.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130625921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of short exposure to electro-oxidation treatment on Planktothrix rubescens","authors":"Maša Čater, T. Rozina, Maja Zupančič Justin","doi":"10.14720/abs.59.1.15702","DOIUrl":"https://doi.org/10.14720/abs.59.1.15702","url":null,"abstract":"Cyanobacterial blooms in fresh water bodies have a serious negative impact on human, animal and environmental health. The impact of electro-oxidation of water containing Planktothrix rubescens in electrolytic cell equipped with diamond electrodes has been tested. The treatment had an immediate effect on cyanobacterial phycocyanin and chlorophyll fluorescence response and total cell biovolume. Cyano-bacterial number reduced for 80 % 130 h after a single treatment of 500 mL of a water sample through electrolytic cell with a flow rate 1 L/min. Obtained results indicate a great potential of the method for in-lake cyanobacterial control.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122419388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Jemec, T. Milivojević, D. Drobne, K. Sepčič, G. Glavan
{"title":"No chronic effects on biochemical biomarkers, feeding and survival of carnolian honeybees (Apis mellifera carnica) after exposure to nanosized carbon black and titanium dioxide","authors":"A. Jemec, T. Milivojević, D. Drobne, K. Sepčič, G. Glavan","doi":"10.14720/abs.59.1.15698","DOIUrl":"https://doi.org/10.14720/abs.59.1.15698","url":null,"abstract":"Honeybees (Apis mellifera) are important pollinators threatened by environmental pollution, plan protection products and other potential contaminants. Due to an extensive predicted use of engineered nanomaterials (NMs) in agriculture the impact on honeybees should be investigated. We studied the 10-days chronic dietary effect of carbon black (CB) and titanium dioxide (TiO2) NMs on the antioxidant activities, cholinergic function, feeding behaviour and survival of honeybees. Exposure of honeybees Apis mellifera carnica to TiO2 and CB NMs (1 mg ml-1) did not affect the feeding and survival. No alteration of catalase, acetylcholinesterase and glutathione S-transferase enzymatic activity was noticed in the brain of honeybees, indicating that TiO2 and CB NMs at the tested exposure dose had no adverse effects on honeybees. Currently predicted environmental concentrations for TiO2 and CB NMs are significantly lower than the concentration tested in the current study. Based on our findings we conclude that the potential use of TiO2 and CB NMs in agriculture is currently safe for honeybees at the tested concentration level and presents potential advantages compared to other NMs with known toxic potential.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126389195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Golob, Srdan Bavdek, Tina Zajc, Franc Janžekovič, Tina Klenovšek
{"title":"Nekatere anatomske značilnosti skeleta sive čaplje, Ardea cinerea","authors":"Z. Golob, Srdan Bavdek, Tina Zajc, Franc Janžekovič, Tina Klenovšek","doi":"10.14720/abs.59.1.15699","DOIUrl":"https://doi.org/10.14720/abs.59.1.15699","url":null,"abstract":"Na štirih skeletih sive čaplje (Ardea cinerea) smo proučili morfološke lastnosti kosti in skeletnih sklopov, predvsem nekatere segmente lobanje, hrbtenice, plečnega obroča, prsnice in medeničnega obroča. Za lobanjo je značilna izrazita kraniofacialna upogibna cona (zonaflexoria craniofacialis), dolga čelnica (os frontale) z izrazito vzdolžno depresijo (depressio frontalis), dolg postorbitalni lok (arcus postor- bitalis), obsežna senčnična jama (fossa temporalis) in dodatna plitka podsenčnična jama (fossa subtemporalis). Parasfenoidni ročaj (rostrumparasphenoidale) je izrazit, krilatki (ossa pterygoidea) pa močni in premi. Pri vratnih vretencih je značilna močnejša kalcifikacija šestega vretencain njegova toga povezanost s petim. V področju notarija (notarium) vretenca niso zraščena: med zadnjim vratnim in prvim prsnim vretencem je jasen sklep. Tudi prva tri prsna vretenca niso medsebojno zraščena, pač pa tesneje povezana v ventralnem delu. Četrto prsno vretence je izrazito pregibno (prosto), kot pri drugih ptičih. Peto prsno vretence je pridruženo sinsakrumu. Ključnici sta koščeno zraščeni v vilice (furcula), njuna apofiza pa tvori sinostozo z vrhom prsničnega gredlja (apex carinae). Krokarnici se končujeta na prsnici vsaka v svojem sklepnem žlebu (sulcus articularis coracoideus), in sicer tako, da je sternalni konec desne krokarnice položen nekoliko preko leve. Na ročaju prsnice (manubriumsterni) manjka notranji trn (spina interna), na njegovem mestu je kratek žleb. Osnovni morfološki opis dopolnjujejo slike kosti oz. skeleta in dva rentgenska posnetka na živi živali, predstavljeni so tudi rezultati meritev posameznih kosti.","PeriodicalId":121848,"journal":{"name":"Acta Biologica Slovenica","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129689314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}