Experimental eye research最新文献

{"title":"Construction of a competing endogenous RNA regulatory network in pterygium and role of hsa_circ_0081682 in fibroblast proliferation, migration, and apoptosis","authors":"Xinyu Tang ,&nbsp;Qiaodan Yang ,&nbsp;Yulian Dou ,&nbsp;Ruiying Zhang ,&nbsp;Ming Yan","doi":"10.1016/j.exer.2025.110365","DOIUrl":"10.1016/j.exer.2025.110365","url":null,"abstract":"<div><div>Pterygium is a fibrovascular growth associated with chronic inflammation, tissue remodeling, and angiogenesis, which invades the cornea. Circular RNAs (circRNAs) are emerging as pivotal role in many diseases, but their role in pterygium remains unclear. We performed circRNA and miRNA expression profiling on pterygium and conjunctival tissues, then the circRNA-miRNA-mRNA regulatory network was constructed. Bioinformatics was used to predict downstream pathways. Pterygium fibroblasts were used for experiments assessing proliferation (CCK8, EdU), migration (wound healing, transwell), and apoptosis (AnnexinV-FITC/PI). We identified 162 differentially expressed circRNAs and 96 miRNAs. Key pathways involved in pterygium pathogenesis, including focal adhesion and PI3K-Akt signaling, were predicted. Hsa_circ_0081862 was downregulated in pterygium tissues and fibroblasts, inhibiting fibroblast proliferation and migration while promoting apoptosis. This research constructed a ceRNA network and identified hsa_circ_0081682 as the potential diagnostic marker for pterygium. This research contributes to the understanding of biochemical basis of pterygium, which may facilitate the development of targeted strategies for its management and prevention.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110365"},"PeriodicalIF":3.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial alteration of metabolites in diabetic cortical cataracts: New insight into lactate","authors":"Pengfei Li ,&nbsp;Miaomiao Wu ,&nbsp;Rong Wang ,&nbsp;Guowei Zhang ,&nbsp;Lihua Kang ,&nbsp;Huaijin Guan ,&nbsp;Min Ji","doi":"10.1016/j.exer.2025.110361","DOIUrl":"10.1016/j.exer.2025.110361","url":null,"abstract":"<div><div>This study aimed to use metabolomics to accurately reveal alterations in metabolites and potential regulatory mechanisms in patients with diabetic cortical cataracts (DCC). We first collected cortical samples from different pathological areas of the same lens in DCC patients for metabolomics. Then, we used transcriptomic analysis to study lactate's effect on gene expression in human lens epithelial cells (HLECs). An in vitro rat lens culture assay evaluated lactate's impact on lens transparency, and WB and immunofluorescence assessed lactate-induced apoptosis and oxidative damage in rat LECs. Furthermore, CHIP sequencing and LC-MS identified H3K18la separately modified genes and potential lactylation proteins in HLECs. Immunoprecipitation validated lactylation levels of proteins. Our findings identified 11 upregulated and 18 downregulated metabolites in the opacity zone of LFCs (OZ-LFCs) compared to the clear zone (CZ-LFCs) in DCC patients. We confirmed the differential lactate content between OZ-LFCs and CZ-LFCs and, through transcriptomic analysis, discovered that lactate affects gene expression, protein metabolism, and DNA repair in primary Human Lens epithelial cells (HLECs). Lactate-induced apoptosis and DNA repair hastened lens opacity in a high-sugar rat lens culture model. Lactylation-MS and H3K18la-ChIP sequencing revealed 591 H3K18la-modified genes and 953 lactylation proteins in HLECs. PKM2 and NPM1 lactylation was confirmed through immunoprecipitation. These findings improve our grasp of spatial dynamics in DCC patient metabolomics and suggest a new research path into lactylation modification to understand lactate's role in cataract formation.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110361"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to the RIMR 2024 special issue","authors":"Marie Csete,&nbsp;Frederick L. Ferris III,&nbsp;SriniVas R. Sadda","doi":"10.1016/j.exer.2025.110354","DOIUrl":"10.1016/j.exer.2025.110354","url":null,"abstract":"","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110354"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilosomes as a novel ocular drug delivery system: Assessing the material attributes, process parameters, and quality attributes","authors":"Nisha Rajbhar ,&nbsp;Devansh Singhal ,&nbsp;Harsh P. Nijhawan ,&nbsp;Piyush Verma ,&nbsp;Govind Soni ,&nbsp;Khushwant S. Yadav","doi":"10.1016/j.exer.2025.110364","DOIUrl":"10.1016/j.exer.2025.110364","url":null,"abstract":"<div><div>Bilosomes are lipidic or surfactant-based nanovesicles with bile salts as key constituents and have emerged as promising carriers for diverse administration routes, including topical, oral, transdermal, and ophthalmic applications. In ocular drug delivery, bilosomes have provided some unique advantages over traditional nano-vesicular systems, which include improved permeation, prolonged retention, enhanced stability, and high deformability, resulting in better drug availability for therapeutic action. This review focuses on the quality attributes and process parameters that govern the design and functionality of bilosomes for ocular drug delivery. By addressing critical material attributes and certain formulation techniques, we provide insights into how these factors influence the stability, permeability, and therapeutic efficacy of bilosome-based systems. Advancements in bilosome formulations for treating ocular disorders, such as glaucoma, bacterial conjunctivitis, and keratitis, are highlighted. Additionally, the potential of surface-modified bilosomes with targeting moieties to enhance drug delivery characteristics is discussed. This review aims to provide a comprehensive overview of bilosome-based approaches, including decorated bilosomes, as a novel strategy for addressing challenges in ocular drug delivery.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110364"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging affects retinal oxygen and blood flow metrics in mice","authors":"Farzan Abdolahi ,&nbsp;Mansour Rahimi ,&nbsp;Rutuja Zinjal ,&nbsp;Sava Sakadzic ,&nbsp;Mahnaz Shahidi","doi":"10.1016/j.exer.2025.110363","DOIUrl":"10.1016/j.exer.2025.110363","url":null,"abstract":"<div><h3>Purpose</h3><div>Aging affects the eyes and is a major risk factor for ocular diseases. The purpose of this study was to determine the effect of age on retinal oxygen and blood flow metrics in mice.</div></div><div><h3>Methods</h3><div>Forty-seven C57BL/6 mice were included in four age groups (3-month-old (m): N = 18, 9m: N = 13, 15m: N = 8, and 21m: N = 8). Retinal arterial and venous diameters (D_A, D_V), venous blood velocity (V_V), and arterial and venous oxygen contents (O_2A, O_2V) were measured by our established multimodal imaging system. Average blood flow (BF), oxygen delivery (DO₂), metabolism (MO₂), and extraction fraction (OEF) were calculated. ANOVA, and Tukey post-hoc analyses were performed to determine the effect of age on variables.</div></div><div><h3>Results</h3><div>We found significant differences in V_V, BF, O_2A, O_2V, and DO₂ among the age groups. V_V was significantly lower in the 21m (<em>p</em> = 0.004) and marginally lower in 15m (<em>p</em> = 0.06) compared to the 3m group, representing reductions of 41% and 30%, respectively. BF was lower in the 21m than the 3m group (<em>p</em> = 0.007), a 42% reduction. Additionally, O_2A and O_2V were significantly lower in 21m compared to the 9m group (<em>p</em> ≤ 0.05), indicating reductions of 30% and 65%, respectively. DO₂ was significantly decreased in the 21m compared to 15m (<em>p</em> = 0.05) and 9m (<em>p</em> = 0.04) groups, representing reductions of 46% and 44%, respectively. Differences in MO₂, OEF, D_A, and D_V did not reach statistical significance (<em>p</em> ≥ 0.07).</div></div><div><h3>Conclusions</h3><div>Our results showed that retinal vascular oxygen content, oxygen delivery rate, and blood flow were significantly reduced in older mice and established baselines for retinal physiological biomarkers according to age.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110363"},"PeriodicalIF":3.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biometric survey of known and prospective murine models of posterior microphthalmia-nanophthalmia","authors":"Navdeep Gogna ,&nbsp;Jai Pinkney ,&nbsp;Lisa Stone,&nbsp;MHD Mustafa Khorzom,&nbsp;Fuxin Zhao ,&nbsp;Gayle B. Collin,&nbsp;Juergen K. Naggert,&nbsp;Mark P. Krebs,&nbsp;Patsy M. Nishina","doi":"10.1016/j.exer.2025.110335","DOIUrl":"10.1016/j.exer.2025.110335","url":null,"abstract":"<div><div>Posterior microphthalmia and nanophthalmia are related genetic conditions that disrupt ocular growth. Here, we conducted a biometric analysis of mouse models to assess shared features of these diseases. Three known microphthalmia alleles (<em>Mfrp</em>^<em>rd6</em>, <em>Prss56</em>^{<em>glcr4</em>}, and <em>Adipor1</em>^{<em>tm1Dgen</em>}) and two prospective alleles (<em>C1qtnf5</em>^{<em>tm1.1(KOMP)Vlcg</em>} and <em>Prss56</em>^{<em>em2(IMPC)J</em>}) were introgressed onto the C57BL/6J (B6) genetic background and compared to B6 mice at 1 through 12 months of age. Biometric parameters obtained using optical coherence tomography were analyzed statistically to identify strain differences. Fundus imaging and histological analyses were performed to assess ocular morphology. <em>Mfrp</em>^<em>rd6</em>, <em>Prss56</em>^{<em>glcr4</em>}, and <em>Prss56</em>^{<em>em2(IMPC)J</em>} mice had significantly shorter axial and posterior lengths, and longer anterior chamber depth compared to controls at all ages studied. <em>Adipor1</em>^{<em>tm1Dgen</em>} mice exhibited similar, but less severe, biometric changes. Axial length was not significantly changed in <em>C1qtnf5</em>^{<em>tm1.1(KOMP)Vlcg</em>} mice, but reduced anterior chamber depth and increased lens thickness were observed at one month of age. Lens and corneal thicknesses were otherwise unchanged in the models as compared to B6 controls. Corneal radius of curvature, examined at 4 months of age, was significantly decreased in all models relative to controls. Micropthalmia was observed independent of retinal degeneration (<em>Mfrp</em>^<em>rd6</em>, <em>Adipor1</em>^{<em>tm1Dgen</em>}) or retinal thickening (<em>Prss56</em> mutants). <em>Prss56</em> mutants developed retinal folds that were absent from other mutants and controls. We conclude that, in mice, <em>Mfrp</em>, <em>Prss56</em>, and <em>Adipor1</em> mutations yield similar microphthalmia phenotypes involving both the anterior and posterior eye. Changes to anterior chamber depth, lens thickness, and corneal curvature in <em>C1qtnf5</em>^{<em>tm1.1(KOMP)Vlcg</em>} mice suggest a role of <em>C1qtnf5</em> in anterior ocular growth.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110335"},"PeriodicalIF":3.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asparagine alleviates naphthalene-induced lens opacity by suppressing ferroptosis","authors":"Tingting Cui ,&nbsp;Ying Liu ,&nbsp;Furong Gao ,&nbsp;Juan Wang ,&nbsp;Lixia Lu ,&nbsp;Jieping Zhang ,&nbsp;Haibin Tian ,&nbsp;Guo-Tong Xu ,&nbsp;Caixia Jin ,&nbsp;Yanlong Bi ,&nbsp;Qingjian Ou ,&nbsp;Jing-Ying Xu","doi":"10.1016/j.exer.2025.110362","DOIUrl":"10.1016/j.exer.2025.110362","url":null,"abstract":"<div><div>Cataract, with lens opacity as its feature, often cause vision loss. The main clinical treatment is lens replacement surgery, which usually works well for most of the patients, but not for all. And researching drugs to delay or treat cataracts is also very important socially and scientifically. This study explored the effect of asparagine (Asn) on cataracts. <em>In vivo</em>, a naphthalene-induced cataract model in rats was set up, focusing on lens opacity. <em>In vitro,</em> SRA01/04 cells or cultured lenses were treated with the naphthalene metabolite 1,2-dihydroxynaphthalene (1,2-DHN) to study cellular mechanisms. The results showed that Asn effectively reduced lens opacity in rats with naphthalene-induced cataracts. <em>In vitro</em> experiments revealed that the ATF3/GPX4 signaling pathway is involved in the mechanism by which asparagine inhibits ferroptosis in lens epithelial cells induced by 1,2-DHN, playing a crucial role in this process. When given orally, Asn could cut down the accumulation of ferrous ions caused by naphthalene, stop the production of reactive oxygen species (ROS) and malondialdehyde (MDA), and ease the depletion of glutathione (GSH). In short, our findings suggest that Asn can protect against naphthalene-induced cataracts by reducing ferroptosis. This new discovery surely creates new research directions and strategies for future cataract prevention and treatment.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110362"},"PeriodicalIF":3.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical insulin treatment efficacy in rat corneal epithelial wound healing model and comparison of efficacy with topical hyaluronate treatment","authors":"Sena Karabay Kılıçarslan ,&nbsp;Gökhan Tortumlu ,&nbsp;Leyla Asena ,&nbsp;Caner İncekaş ,&nbsp;Fatma Helvacıoğlu ,&nbsp;Dilek Dursun Altınörs","doi":"10.1016/j.exer.2025.110359","DOIUrl":"10.1016/j.exer.2025.110359","url":null,"abstract":"<div><div>This study aimed to examine the effect of topical insulin on healing of the corneal epithelium in a rat corneal epithelial defect model, comparing it to artificial tears and non-treated controls. 28 adult male Sprague Dawley rats were divided into 4 groups in this study. A 2 mm diameter epithelial defect was created at the central cornea of one eye under sedation. Group A had untreated epithelial defects, Group B received 0.15 % sodium hyaluronate qid, and Group C received topical 1IU/mL insulin qid. The last group was healthy controls. Biomicroscopic examinations were performed at the 4th hour, 12th hour, 1st day, 2nd day, and 3rd day0. The defect size of epithelium was photographed during each examination using fluorescein staining. The area of the defect was calculated using the ImageJ software to determine the percentage of defect closure. On the 10th day, corneal tissues were excised for histopathological examination, after sacrification. Topical insulin and sodium hyaluronate treatment groups showed faster healing rate of corneal epithelial defect closure biomicroscopically compared to the untreated group. However, there was no statistically significant difference in the rate of epithelial defect closure between topical insulin and sodium hyaluronate groups. Histopathologically, topical insulin group showed superior epithelial remodeling at the fine structural level. Although no biomicroscopic difference was observed in corneal epithelial healing with topical insulin treatment compared to sodium hyaluronate, it has been demonstrated to be more effective histopathologically.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110359"},"PeriodicalIF":3.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic intermittent hypoxia modulates corneal fibrotic markers and inflammatory cytokine expression in a sex-dependent manner","authors":"Jessica L. Bradshaw ,&nbsp;Brenda Vasini ,&nbsp;Steve Mabry ,&nbsp;Brenna S. Hefley ,&nbsp;E. Nicole Wilson ,&nbsp;Jennifer J. Gardner ,&nbsp;Rebecca L. Cunningham ,&nbsp;Dimitrios Karamichos","doi":"10.1016/j.exer.2025.110358","DOIUrl":"10.1016/j.exer.2025.110358","url":null,"abstract":"<div><div>Chronic intermittent hypoxia (CIH) is a commonly observed condition in patients suffering from obstructive sleep apnea (OSA). Previous studies link CIH to fibrosis, inflammation, and hormonal dysregulation across various tissues. Yet, the effect of CIH in the cornea is unknown. Moreover, women and men diagnosed with OSA present with diverse symptoms, suggesting sex-specific pathophysiology at play. Thus, we used a rat model to assess the impact of CIH and sex on protein expression of corneal fibrotic markers (α-SMA, COL III, cFN, TSP-1), proinflammatory cytokines (IL-1 α, IL-17, IL-18, and IFN-γ), and hormone receptors (ERα, ERβ, GPER, GnRH-R, and LH-R). Male and female Sprague Dawley rats were exposed to normoxic or CIH conditions during their sleep cycle for 14 days. Extracted corneal proteins were subjected to Western blot and multiplex magnetic bead analysis. Our results reveal sex differences in fibrotic and inflammatory markers in the cornea, whereby female corneas exhibit higher levels of fibrotic markers, while male corneas exhibit increased inflammatory cytokines. CIH exposure resulted in elevated levels of α-SMA and pro-inflammatory cytokines in female corneas, while there was no impact on fibrotic or inflammatory markers in male corneas. Additionally, CIH exposure reduced hormone receptors in male and female corneas in a sex-dependent manner. Correlation analyses identified associations of corneal hormone receptors with corneal fibrotic and pro-inflammatory markers that were dependent on sex, with female corneas demonstrating stronger correlations compared to male corneas. Altogether, our data suggests hormone-mediated signaling may contribute to CIH-mediated corneal fibrosis and inflammatory phenotypes, especially in females.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110358"},"PeriodicalIF":3.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermidine mitigates blue-light-induced corneal injury by alleviating oxidative stress and restoring autophagy impairment","authors":"Xiang Li ,&nbsp;Jiayun Ge ,&nbsp;Jiru Zhu ,&nbsp;Xin Yu ,&nbsp;Dongjie Song ,&nbsp;Zongchan Zhang ,&nbsp;Yutong Xia ,&nbsp;Yanqing Li ,&nbsp;Zhitong Chen ,&nbsp;Kuangqi Chen ,&nbsp;Ye Shen ,&nbsp;Jianping Tong","doi":"10.1016/j.exer.2025.110360","DOIUrl":"10.1016/j.exer.2025.110360","url":null,"abstract":"<div><div>This study investigated the protective efficacy of spermidine on corneal epithelium exposed to photodamage from artificial blue light (BL). After six weeks of BL exposure, the rabbit cornea exhibited epithelial injury and delayed wound healing accompanied by downregulation of spermidine, as identified through metabolomic analysis. Pathways related to autophagy and the antioxidant response were implicated in this process. <em>In vitro</em>, spermidine mitigated BL-induced reductions in viability and proliferation of human corneal epithelial cells, decreased cell death, and enhanced colony formation. Spermidine also partially reversed BL-induced mitochondrial dysfunction, oxidative stress and autophagy impairment. Furthermore, topical administration of spermidine eye drops reduced epithelial injury in the rabbit cornea under BL exposure, demonstrating benefits in promoting cell proliferation, accelerating wound healing, and maintaining antioxidant capacity and autophagy.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110360"},"PeriodicalIF":3.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}