Experimental and Clinical Physiology and Biochemistry最新文献

{"title":"High content of dietary fructose stimulates the formation of neutrophil extracellular traps in the biliary system","authors":"І. Біла, О. І. Віщур, Р. О. Білий","doi":"10.25040/ecpb2020.01.029","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.029","url":null,"abstract":"Neutrophils safeguard our bloodstreams as well as the surface of ducts, mucosa linings and wounds, intending to protect us from pathogens. Bile ducts connect the bacteria-filled intestine with the liver, the metabolic factory of the body, full of sinusoidal capillaries. Thus, bile ducts become a hot place in the body, requiring constant protection from pathogens. Neutrophils can us three ways: they can phagocytize pathogens, produce chemicals mediate pathogens` to form neutrophil extracellular traps (NETs). The latter decondensed DNA fibers, decorated components of neutrophilic granules abundant ROS production. NETs the but, and examined for the presence of NETs by visualizing DNA content with PI by means of the fluorescence microscopy. Under both HFD and HLHCD conditions we observed the formation of NETs in the gallbladder. Serum, obtained from blood samples, was analyzed for the activity of neutrophil elastase (an enzyme released from neutrophilic granules upon NETs formation) by using specific fluorogenic substrate. Neutrophil elastase activity was significantly increased after 6 weeks of feeding on the HFD (p=0.0219) and HLHCD (p=0.0148, ANOVA). Thus, we can conclude that high dietary fructose content stimulates the formation of neutrophil extracellular traps in bile ducts of experimental animals. These findings are extremely important in understanding the reasons for low-grade inflammation observed under non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH).","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77555548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The variety of immune responses to adoptogenetic balneotherapy is strictly deterministic","authors":"O. Melnyk, Z. Struk","doi":"10.25040/ecpb2020.01.005","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.005","url":null,"abstract":"Four variants of the immune responses to adaptogenic balneotherapy have been identified. It is shown that the immunotropic effect of balneotherapy on certain individuals is not effec tive enough and in some cases even unfavorable. All four variants of immune responses are virtually unmistakably predicted by a set of 20 immune and microbiota predictors. Each type of immune response is characterized by a specific vegetative, endocrine and metabolic support. In the next study the discriminant analysis revealed that the constellation 8 HRV and 5 endo crine parameters as well as gender of the patient predicts the nature of the immune response with an accuracy of 90,9%. As soon as we found out 16 metabolic parameters as the predictors which, taken together, determine the nature of the immune response with an accuracy of 95,5%. Standing on the positions of determinism, we assumed that taking into account all the recorded initial neuro-endocrine and metabolic parameters of the body would allow to predict the immune response of each patient unmistakably. Testing this hypothesis was the purpose of this study. The object of observation was 34 men and 10 women aged 24-70, who came to the Truskavets’ spa for the treatment of chronic pyelonephritis combined with cholecystitis in remission. The state of the autonomic nervous system is estimated by parameters of HRV (“CardioLab+HRV”). To assess the endocrine status we determined plasma levels of principal adaptation hormones: Cortisol, Testosterone and Triiodothyronine (ELISA with the use of analyzer “RT-2100C”). The metabolic panel amounted to: plasma lipoproteines; the plasma and daily urine levels of the electrolytes (calcium, magnesium, phosphates, chloride, sodium and potassium; nitrous metabo lites (creatinine, urea, uric acid). According to the parameters of electrolyte exchange, hormonal activity was evaluated: parathyroid, calcitonin and mineralocorticoid. Following the accepted algorithm, the forward stepwise method revealed 31 parameters as the predictors, among them: 13 HRVs, 4 endocrine, 11 metabolic as well as 2 parameters of cholekinetics and age. All types of immune response to adaptogenic balneotherapy are predicted unmistakably. The previously revealed variety of immune responses to adaptogenic balneotherapy is quite strictly conditioned by the initial state of the neuroendocrine-immune complex, microbiota, me tabolism as well as the sex and age of patients.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82483413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protector properties of N-acetylcysteine, melatonin and their compatible use for oxidative damage of rat brain cells with experimental diabetes mellitus type 1","authors":"","doi":"10.25040/ecpb2019.04.016","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.016","url":null,"abstract":"Diabetes mellitus (DM) is the most widespread endocrinological disease which associated with increasing risk of Alzheimer’s disease. Diabetic encephalopathy is one of the most common and serious complications of DM. Molecular mechanisms of diabetic encephalopathy are inves-tigated. An important element in the development of DM1 encephalopathy is the activation of oxidative stress. And determination the levels of “free iron” and 8-oxoguanine are important for evaluation of the effectiveness of diabetic encephalopathy therapy. Purpose of the study. To study the effect of N-acetylcysteine, melatonin and their compatible use on the state of oxidative damage of rat brain cells with experimental DM1. Materials and research methods. Experiments were carried out on male Wistar rats. DM1 was induced by administration of streptozotocin (STZ). Rats with induced DM1 was re-ceiving N-acetylcysteine (NAC, 1500 mg/kg), melatonin (Mel, 10 mg/kg) and a combination during 5 weeks, starting at 15 days after control pathology was reproduced. Euthanasia was implemented by decapitation under thiopental anesthesia (40 mg/kg, intraperitoneally) to col-lect blood and brain of rats. Molecular determined by spectrophotometrically. Levels of “free iron” were determined by electron paramagnetic resonance (EPR) on a computerized EPR-spectrophotometer PE-1307. Results and its discussion. During 7-week experiment, the levels of “free iron” in brain tissue and blood in group of animals with DM1 were significantly higher than corresponding values of control group (by 17-times and by 8-times р< 0,05). Under these conditions, the marker of oxidatively damaged DNA, 8-oxoG, significantly raised in brain of diabetic rats by 3.4 times more than in control group (0.61±0.10 nm/g vs 0.18±0.06 nm/g, р< 0,05). The induction of NAC, Mel, and especially their combination, was accompanied with decrease in “free iron” complexes in brain tissues (by 2.1-8.5-times) and blood (by 1.2-1.4-times) of rats with experimental DM1. 0,05). significant 2.0-fold decrease of 8-oxoG Conclusions. Induction of DM1 contributes to the intensification of oxidation processes, which is accompanied by an increasing in the levels of “free iron” complexes in the tissues of the brain and of experimental Under these conditions, oxidative damage to was observed, as evidenced by increasing in the level of 8-oxoG (p <0.05). The induction of N-acetylcysteine, melatonin, and especially their combination, contributed to the antioxidant protection of rat brain cells with experimental DM1, reducing the level of “free iron” and coun-teracting oxidative DNA damage.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81223667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of physical rehabilitation on the quality of life in patients with rheumatoid arthritis","authors":"","doi":"10.25040/ecpb2019.04.045","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.045","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85638177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIGUANIDES AND IMMUNE CHANGES IN PATIENTS WITH SYSTEMIC CONNECTIVE TISSUE DISEASES WITH A METABOLIC SYNDROME","authors":"","doi":"10.25040/ecpb2019.04.029","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.029","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87303136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoretical and applied aspects of the application of alternative methods of toxicity research in the implementation of hygienic regulation of chemical factors of production","authors":"","doi":"10.25040/ecpb2019.04.073","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.073","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"204 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74460703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Nitric Oxide Synthesis Modulators on the Mechanisms of Apoptosis Development and Production of Reactive Oxygen Species in the Blood Leukocytes in Experimental Antiphospholipid Syndrome","authors":"","doi":"10.25040/ecpb2019.04.053","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.053","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79367377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian cancer: Ca2+-dependent and Ca2+-independent isoforms of NO-synthase functioning in blood lymphocytes of women","authors":"","doi":"10.25040/ecpb2019.04.063","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.063","url":null,"abstract":"The purpose of the work was to study the activity and kinetic parameters of isoforms of NO-synthase blood lymphocytes in women with ovarian cancer. Ovarian cancer is considered to be one of the most severe diseases of the female reproductive tract. The study of the pathogenesis of ovarian cancer is being directed to improve methods of diagnosis and detection of tumor formation process at an early stages. It is known that metabolism of L -arginine and synthesis of nitric oxide (NO) plays an important role in carcinogenesis and tumor growth. It is assumed that NO modulates can-cer-dependent events, including angiogenesis, apoptosis, cell cycle, invasion imaginary affinity constant inducible isoform of NO-synthase to arginine was in 5.4 times lower than that of constitutive isoforms of NO-synthase of blood lymphocytes of the control group and inhibition of constitutive isoform occurs by competitive type –by reducing the rate of enzyme turnover.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"257 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76206523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the hemodynamics of athletes under the influence of anaerobic physical tests of different duration","authors":"","doi":"10.25040/ecpb2019.04.038","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.038","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82355968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Biochemical Parameters of Blood and Homogenate of the Alveolar Bone of Female Rats at Long-term Experimental Methionine Hyperhomocysteinemia and its Correction with Choline and Vitamin D","authors":"І. Кутельмах","doi":"10.25040/ecpb2019.04.022","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.022","url":null,"abstract":"bone homogenate of the experimental animals, the concentration of total protein, malondial-dehyde, reduced glutathione, phospholipids and their fractional composition, total and ionized calcium and phosphate content, alkaline acid phosphatase, alpha phosphate activity, alkaline phosphatase activity, and alpha phosphate activity were determined, content of homocysteine and 25-hydroxyvitamin D (25-OH D). on the female rats on the biochemical processes in the blood and the dental status of their progeny, in the early postnatal period of life.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86829318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}