European Journal of Plant Pathology最新文献_第9页

Fungicides control black rot in Vanda: a strategy to avoid fungicide resistance 杀菌剂控制万达黑腐病：避免杀菌剂产生抗药性的策略

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-26 DOI: 10.1007/s10658-024-02824-1

Onuma Piasai, Tanapol Anyong, Netnapis Khewkhom, Wiphawee Leesutthiphonchai

{"title":"Fungicides control black rot in Vanda: a strategy to avoid fungicide resistance","authors":"Onuma Piasai, Tanapol Anyong, Netnapis Khewkhom, Wiphawee Leesutthiphonchai","doi":"10.1007/s10658-024-02824-1","DOIUrl":"https://doi.org/10.1007/s10658-024-02824-1","url":null,"abstract":"Vanda is a highly valued and important orchid in the horticultural industry; however, it is usually damaged by black rot disease, caused by Phytophthora species, leading to substantial economic losses. This study aims to evaluate multiple fungicides for black rot control. Oomycetes were isolated from diseased plants, and these isolates brought about a 100% disease incidence in Vanda with water-soaked lesions, becoming necrotic within 24 h. The isolates were identified as Phytophthora palmivora based on a phylogenetic analysis of internal transcribed spacers (ITSs) and cytochrome oxidase spacer regions. For controlling black rot disease, eight fungicides or mixtures with different target sites and modes of action were screened, including cyazofamid, dimethomorph, fosetyl-aluminum, metalaxyl, thiophanate-methyl, a mixture of fluopicolide and fosetyl-aluminum, a mixture of mancozeb and valifenalate, and a mixture of quintozene and etridiazole. All tested fungicides showed 33%–100% growth inhibition using the poisoned food technique. In greenhouse experiments, seven tested fungicide formulations, except for thiophanate-methyl, exhibited 20%–66% reduced lesion sizes compared with the no-chemical control. The information on fungicides with different filicide group codes could assist a fungicide spray program for black rot management in orchids.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"136 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139586180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Crotalaria spectabilis extract in Meloidogyne javanica and Heterodera glycines soybean nematodes Crotalaria spectabilis 提取物对 Meloidogyne javanica 和 Heterodera glycines 大豆线虫的影响

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-24 DOI: 10.1007/s10658-024-02821-4

Tamires Scupinari, Helena Mannochio-Russo, Gustavo Galo Marcheafave, Vanderlan da Silva Bolzani, Estela de Oliveira Nunes, Waldir Pereira Dias, Clara Beatriz Hoffmann-Campo, Maria Luiza Zeraik

{"title":"Effects of Crotalaria spectabilis extract in Meloidogyne javanica and Heterodera glycines soybean nematodes","authors":"Tamires Scupinari, Helena Mannochio-Russo, Gustavo Galo Marcheafave, Vanderlan da Silva Bolzani, Estela de Oliveira Nunes, Waldir Pereira Dias, Clara Beatriz Hoffmann-Campo, Maria Luiza Zeraik","doi":"10.1007/s10658-024-02821-4","DOIUrl":"https://doi.org/10.1007/s10658-024-02821-4","url":null,"abstract":"Nematodes are a common soybean threat, and the search for new solutions for controlling them has been the focus of many studies worldwide. Crotalaria spp. are known sources of pyrrolizidine alkaloids with nematicidal activities. In this study, the nematicidal and nematostatic effects of C. spectabilis extracts were evaluated in vitro over two different nematode species (Heterodera glycines and Meloidogyne javanica). A full factorial experimental design was employed to understand the effects and interactions of input factors (plant organ, extraction solvent, and nematode species) on the output response (nematode mortality). Ethanolic and alkaline extracts of leaves and seeds were tested at concentrations of 5, 10 and 20 mg L-1 in bioassays with 48 hours of incubation. The C. spectabilis seed’s alkaline extract assessed for H. glycines resulted in higher mortality rates (close to 96%) compared to the other experiments. Therefore, this combination was also evaluated in vivo in a soybean plantation, resulting in a decrease of about 90% in the nematode multiplication factor compared to the control group. Ultra-High Performance Liquid Chromatography coupled to a tandem High-Resolution Mass Spectrometer (UHPLC-HRMS/MS) analyses revealed pyrrolizidine alkaloids in the extract, such as senecionine N-oxide, besides monocrotaline and its derivatives. The results obtained in this study allow new possibilities to handle nematode infections. The direct application of C. spectabilis extracts or their isolated bioactive compounds on soil can be a promising approach.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139561725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Timing is everything: how planting period shapes nutritional quality, mycobiota characteristics, and mycotoxin contamination in maize (Zea mays) grains 时间就是一切：种植期如何影响玉米（Zea mays）谷物的营养质量、霉菌生物群特征和霉菌毒素污染

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-24 DOI: 10.1007/s10658-024-02820-5

{"title":"Timing is everything: how planting period shapes nutritional quality, mycobiota characteristics, and mycotoxin contamination in maize (Zea mays) grains","authors":"","doi":"10.1007/s10658-024-02820-5","DOIUrl":"https://doi.org/10.1007/s10658-024-02820-5","url":null,"abstract":"<h3>Abstract</h3> Maize (Zea mays L.) is crucial in global grain markets and food/feed production. Fungal contamination in ears can adversely affect crop yield and diminish the nutritional value of grains. Moreover, many of the main pathogens affecting maize are producers of mycotoxins, which pose a risk to food safety. The area under late-planted maize in Argentina has increased, as more stable yields despite lower potential are expected in comparison to the early planting period. However, late-planted maize is prone to fungal infections and insect damage as well as mycotoxin contamination. This study aimed to evaluate the influence of maize planting periods (early vs. late) on grain nutritional quality, grain mycobiota, mycotoxin levels, and their interrelationships. Two experiments were conducted at Buenos Aires (Argentina) using thirteen genetically stable maize hybrids. Early planting occurred on October 5 (2020) whereas late planting was performed on December 12 (2020). Grain yield, number of grains per square meter, and thousand grain weight were measured at harvest and representative samples of grains were used for the laboratory analyses. The results demonstrated that early-planted maize exhibited high grain nutritional quality and low levels of toxigenic fungal mycobiota. In contrast, late-planted maize displayed low nutritional quality, increased presence of toxigenic mycobiota, and high contamination with elevated levels of fumonisins (a major mycotoxin). This study highlights the impact of planting period on grain yield, grain quality, and mycotoxin contamination, and it emphasizes the need for more research and regulatory considerations in this field. <h3>Graphical Abstract</h3> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/10658_2024_2820_Figa_HTML.png\"/> ","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"54 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139561848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cactodera guizhouensis n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode parasitizing potato in Guizhou, China Cactodera guizhouensis n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode parasitizing potato in Guizhou, China

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-24 DOI: 10.1007/s10658-024-02816-1

Chun-Hui Ni, Yu-Jia Xie, Si-Hua Yang, Zai-Fu Yang, Chun-Ling Xu, Hui Xie

{"title":"Cactodera guizhouensis n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode parasitizing potato in Guizhou, China","authors":"Chun-Hui Ni, Yu-Jia Xie, Si-Hua Yang, Zai-Fu Yang, Chun-Ling Xu, Hui Xie","doi":"10.1007/s10658-024-02816-1","DOIUrl":"https://doi.org/10.1007/s10658-024-02816-1","url":null,"abstract":"A new cyst-forming nematode, Cactodera guizhouensis n. sp., isolated from the roots and surrounding soil of potato in Guizhou, China, is the first species of genus Cactodera found from the potato rhizosphere. The new species was characterized by the L/W ratio of its cyst, being 1.4 ± 0.1 (1.3–1.8), with a fenestral diameter of 17.7–28.7 × 12.3–23.4 μm and being absent vulval denticlest; the stylet length of second stage juveniles is 26.5 ± 1.2 (24.1–29.0) μm, while the tail is 53.4 ± 3.9 (45.0–61.9) μm and the hyaline tail is 25.7 ± 2.4 (21.6–29.8) μm, the lateral field having four incisures and areolation; the eggshell having punctations. The internal transcribed spacer sequences of ribosomal RNA (ITS-rRNA), and sequences of the D2-D3 region of 28S ribosomal RNA (28S-rRNA) from the new species were amplified and sequenced. The phylogenetic tree based on the sequences showed that the new species clustered into a separate clade and could be distinguished from other species in genus Cactodera. A key to the species of Cactodera is also provided in this study.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"25 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139561829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative characterization and phylogenetic relationships of Xiphinema rivesi and X. pachtaicum (Nematoda, Longidoridae) associated to vineyards in North Italy 与意大利北部葡萄园相关的 Xiphinema rivesi 和 X. pachtaicum（线虫纲，长虫科）的综合特征和系统发育关系

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-18 DOI: 10.1007/s10658-024-02815-2

Alberto Troccoli, Alessio Vovlas, Elena Fanelli, Valentina Papeschi, Stefano Toninelli, Trifone D’Addabbo, Francesca De Luca

{"title":"Integrative characterization and phylogenetic relationships of Xiphinema rivesi and X. pachtaicum (Nematoda, Longidoridae) associated to vineyards in North Italy","authors":"Alberto Troccoli, Alessio Vovlas, Elena Fanelli, Valentina Papeschi, Stefano Toninelli, Trifone D’Addabbo, Francesca De Luca","doi":"10.1007/s10658-024-02815-2","DOIUrl":"https://doi.org/10.1007/s10658-024-02815-2","url":null,"abstract":"The occurrence of Xiphinema species in the rhizosphere of vineyards showing general decline phenotype in North Italy was investigated. Most of the collected soil samples contained two Xiphinema species belonging to X. americanum-group, along with X. index. Using the integrative taxonomy approach, combining morphological, molecular and multivariate morphological analyses, the two populations of the X. americanum-group were identified as X. rivesi and X. pachtaicum. Furthermore, one male specimen of X. rivesi was recovered and the presence of three juvenile developmental stages was confirmed based on the scatter diagram. D2-D3 expansion domains of LSU, ITS region and partial COI were used to determine the phylogenetic relationships of the two Xiphinema species from North Italy with other species belonging to X. americanum-group. Five other Xiphinema populations from different geographical areas were included in this study and only molecularly identified as X. rivesi, X. pachtaicum and X. incertum by sequencing the ITS and D2-D3 expansion domains of LSU rDNA.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"234 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139499065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of RNA interference targeting chemosensory genes on chemotactic host-seeking in Meloidogyne graminicola 以化感基因为靶点的 RNA 干扰对禾谷圆线虫趋化性宿主寻找的影响

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-15 DOI: 10.1007/s10658-024-02818-z

Di Wang, Xuqi Shi, Xiping Long, Zixu Zhang, Shan Ye, Zhong Ding, Zhuhong Yang

{"title":"Effect of RNA interference targeting chemosensory genes on chemotactic host-seeking in Meloidogyne graminicola","authors":"Di Wang, Xuqi Shi, Xiping Long, Zixu Zhang, Shan Ye, Zhong Ding, Zhuhong Yang","doi":"10.1007/s10658-024-02818-z","DOIUrl":"https://doi.org/10.1007/s10658-024-02818-z","url":null,"abstract":"Pre-parasitic second-stage juveniles (pre-J2s) of Meloidogyne graminicola are non-feeding in soil, and their migration, localization and successful invasion of host roots are essential for survival. Chemotaxis is thought to play a central role in host localization, but the mechanisms of chemotaxis are poorly understood. In this study, we cloned and evaluated the molecular characteristics and functions of four chemosensory genes, including Mg-odr-3, Mg-odr-1, Mg-tax-4, and Mg-osm-9. Quantitative real-time PCR (qRT‒PCR) analysis revealed that Mg-odr-3 and Mg-osm-9 tended to be expressed in pre-J2s and parasitic J2s (par-J2s), while Mg-odr-1 and Mg-tax-4 were expressed in par-J2s, consistent with their functions during the host-seeking period and establishment of suitable feeding sites in root tips. Root signals from eight plant root extracts, namely, rice, barnyard grass (Echinochloa crus-galli), wheat, soybean, pepper, tomato, eggplant, and marigold, were repulsive to J2s of M. graminicola. Moreover, RNAi silencing of Mg-odr-3, Mg-odr-1, Mg-tax-4, and Mg-osm-9 in J2s attenuated their repulsion to the rice root extract. Furthermore, we found that the response of J2s to root exudates was concentration-dependent, with high concentrations of root exudates repelling J2s. Silenced J2s also showed reduced repulsion in response to high concentrations of rice root exudates. Conversely, M. graminicola J2s were attracted to the root tips of the eight plants, while RNAi-J2s showed defective attraction to root tips. In conclusion, our results showed that chemosensory genes are crucial for the chemotactic host-seeking behaviour of M. graminicola.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"30 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139474853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of bacteria causing Acute Oak Decline on Quercus robur in Slovakia 斯洛伐克首次报告导致栎树急性衰退的细菌

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-09 DOI: 10.1007/s10658-024-02813-4

Miłosz Tkaczyk, Katarzyna Sikora, Juraj Galko

引用次数: 0

Cadophora luteo-olivacea on apple and kiwifruit: characterization of selected strains and evaluation of fungicides for their control 苹果和猕猴桃上的 Cadophora luteo-olivacea：选定菌株的特征和杀真菌剂对其控制的评估

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2024-01-09 DOI: 10.1007/s10658-023-02811-y

S. Tomada, E. Staffler, G. Dionis, S. Baric, A. Di Francesco

{"title":"Cadophora luteo-olivacea on apple and kiwifruit: characterization of selected strains and evaluation of fungicides for their control","authors":"S. Tomada, E. Staffler, G. Dionis, S. Baric, A. Di Francesco","doi":"10.1007/s10658-023-02811-y","DOIUrl":"https://doi.org/10.1007/s10658-023-02811-y","url":null,"abstract":"Cadophora luteo-olivacea has been reported as an emerging postharvest pathogen of pome fruit and kiwifruit with the potential to have a significant economic impact. To date, the biology and epidemiology of C. luteo-olivacea has been poorly investigated. Therefore, the present study aims to gain knowledge on C. luteo-olivacea biology, by analyzing the parameters that can influence the fungal growth and virulence and by getting information about the sensitivity to fungicides. A mycelial growth study at different temperatures was conducted with five C. luteo-olivacea isolates belonging to different host plants (apple and kiwifruit). The optimum fungal growth was observed in a temperature range between 20 °C and 25 °C, with C. luteo-olivacea isolates from kiwifruit that resulted in faster growth than apple isolates. The pathogenicity of C. luteo-olivacea isolates was evaluated on detached apple and kiwifruit twigs, on 'Golden Delicious' and 'Fuji' apples, and on 'Hayward' and 'Sungold' kiwifruit, stored both at 0 °C and 20 °C. The pathogenicity on fruit and on woody twigs was variable, depending on the host cultivar, with a minor effect related to the fungal isolate. Moreover, the efficacy of ten different plant protection products (PPPs) against the conidial germination of the isolates was determined. Few PPPs were found to be effective (e.g. fludioxonil, dithianon, and cyprodinil) against C. luteo-olivacea. These results represent a starting point for further research on the biology and epidemiology of C. luteo-olivacea and the development of effective management strategies.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"9 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139408112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of plant extracts in growth promotion and onion purple blotch management: Unveiling metabolite fingerprinting of promising neem leaf extracts through GC MS 植物提取物在促进生长和治理洋葱紫斑病方面的功效：通过气相色谱质谱揭示楝树叶提取物的代谢物指纹图谱

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2023-12-30 DOI: 10.1007/s10658-023-02810-z

Shubham Saini, Kushal Raj, Anil Kumar Saini, Rakesh Kumar Chugh, Makhan Lal, Mukul Kumar Bhambhu

{"title":"Efficacy of plant extracts in growth promotion and onion purple blotch management: Unveiling metabolite fingerprinting of promising neem leaf extracts through GC MS","authors":"Shubham Saini, Kushal Raj, Anil Kumar Saini, Rakesh Kumar Chugh, Makhan Lal, Mukul Kumar Bhambhu","doi":"10.1007/s10658-023-02810-z","DOIUrl":"https://doi.org/10.1007/s10658-023-02810-z","url":null,"abstract":"Onion is affected by several biotic and abiotic stresses. This study aimed to evaluate the impact of onion seed priming with different plant extracts. The results show that neem leaf extract treated seeds exhibited the highest mean seedling establishment (71.50 per cent), seedling length (11.21 cm), seedling dry weight (15.88 mg) and seedling vigour indices (800.70 and 1135.27). The biochemical analysis of primed seedlings showed significant enhancement in enzymatic and non-enzymatic antioxidants. Purple blotch caused by Alternaria porri is the most important foliar disease of onion leading to significant losses. The evaluation of plant extracts against Alternaria porri showed that neem leaf extract at a concentration of 25 per cent inhibits mycelial growth of pathogen by 81.18 per cent. The GC MS analysis of the crude extract of neem indicated the presence of forty different chemical compounds, among which the main components included Benzene, 1,4-bis (phenylmethyl)- (15.05%), Naphthalene, 1,6-dimethyl- (10.98%), 3-(2,4-Dimethoxyphenyl) butan-2-one (8.63%) and Phytol (7.99%). Evaluation of the four promising botanicals viz., neem, congress grass, turmeric and ginger under field conditions indicated foliar sprays of neem leaf extract to be effective in both managing purple blotch disease (23.08 per cent disease management) and enhancing yield attributes (5.24 per cent seed yield increase). This study establishes the antimycotic and plant growth promoting potential of neem leaf extract for managing onion purple blotch.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"23 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139064230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture media and temperature effects on Neofusicoccum mediterraneum: assessing mycelial growth and colony characteristics 培养基和温度对地中海新球藻的影响：评估菌丝生长和菌落特征

IF 1.8 3区农林科学

European Journal of Plant Pathology Pub Date : 2023-12-18 DOI: 10.1007/s10658-023-02806-9

Farhat Ali Abouzkhar, Islem Yangui, Sawssen Hlaiem, Olfa Ezzine, Mohamed Lahbib Ben Jamâa

{"title":"Culture media and temperature effects on Neofusicoccum mediterraneum: assessing mycelial growth and colony characteristics","authors":"Farhat Ali Abouzkhar, Islem Yangui, Sawssen Hlaiem, Olfa Ezzine, Mohamed Lahbib Ben Jamâa","doi":"10.1007/s10658-023-02806-9","DOIUrl":"https://doi.org/10.1007/s10658-023-02806-9","url":null,"abstract":"In this study, Neofusicoccum mediterraneum, a highly aggressive plant pathogen with a broad host range, including Eucalyptus spp., was investigated to determine its optimum medium and temperature for mycelial growth and chlamydospore production. The results revealed differences in the growth behaviour of N. mediterraneum under different in vitro conditions. Potato Sucrose Agar (PSA) was determined to be the most favourable environment for mycelial growth, achieving the maximum growth rate (GR) of 100% after 3 days of incubation at 25 °C. This was closely followed by cultures on Malt Extract Agar (MEA), exhibiting a GR of 100% after 4 days of incubation at 25 °C, while both substrates displayed optimal growth within the temperature range of 25 to 30 °C. The General Linear Model (GLM) unveiled significant differences in the growth rate across the different media (P = 0.000) and temperatures (P = 0.000). The interaction effect (media*temperature) retained its high significance throughout all incubation days (P < 0.0001). The binomial distribution and multinomial logistic regression analysis revealed that colony texture was significantly influenced by both the media (P = 0.022) and the temperature (P = 0.008). Additionally, temperature affected significantly the surface colour (P = 0.015) and reverse colour (P = 0.023) of the colony. Production of chlamydospores was observed only on PSA at 20 °C, 25 °C, 30 °C and 35 °C and on PDA at 25 °C and 30 °C. Both the medium and temperature had notable influences on the pathogen's growth, highlighting their importance in understanding its biology and behaviour. These findings contribute to the understanding of the phenotypic plasticity exhibited by N. mediterraneum towards temperatures and growth substrates.","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":"32 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0