European journal of cell biology最新文献

{"title":"Damage control of epithelial barrier function in dynamic environments","authors":"Tomohito Higashi,&nbsp;Akira C. Saito,&nbsp;Hideki Chiba","doi":"10.1016/j.ejcb.2024.151410","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151410","url":null,"abstract":"<div><p>Epithelial tissues cover the surfaces and lumens of the internal organs of multicellular animals and crucially contribute to internal environment homeostasis by delineating distinct compartments within the body. This vital role is known as epithelial barrier function. Epithelial cells are arranged like cobblestones and intricately bind together to form an epithelial sheet that upholds this barrier function. Central to the restriction of solute and fluid diffusion through intercellular spaces are occluding junctions, tight junctions in vertebrates and septate junctions in invertebrates. As part of epithelial tissues, cells undergo constant renewal, with older cells being replaced by new ones. Simultaneously, the epithelial tissue undergoes relative rearrangement, elongating, and shifting directionally as a whole. The movement or shape changes within the epithelial sheet necessitate significant deformation and reconnection of occluding junctions. Recent advancements have shed light on the intricate mechanisms through which epithelial cells sustain their barrier function in dynamic environments. This review aims to introduce these noteworthy findings and discuss some of the questions that remain unanswered.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151410"},"PeriodicalIF":6.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S017193352400027X/pdfft?md5=6566a5c09602d1fd655944353375b58b&pid=1-s2.0-S017193352400027X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140344911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cortactin interacts with αDystrobrevin-1 and regulates murine neuromuscular junction morphology","authors":"Teresa De Cicco ,&nbsp;Marcin Pęziński ,&nbsp;Olga Wójcicka ,&nbsp;Bhola Shankar Pradhan ,&nbsp;Margareta Jabłońska ,&nbsp;Klemens Rottner ,&nbsp;Tomasz J. Prószyński","doi":"10.1016/j.ejcb.2024.151409","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151409","url":null,"abstract":"<div><p>Neuromuscular junctions transmit signals from the nervous system to skeletal muscles, triggering their contraction, and their proper organization is essential for breathing and voluntary movements. αDystrobrevin-1 is a cytoplasmic component of the dystrophin-glycoprotein complex and has pivotal functions in regulating the integrity of muscle fibers and neuromuscular junctions. Previous studies identified that αDystrobrevin-1 functions in the organization of the neuromuscular junction and that its phosphorylation in the C-terminus is required in this process. Our proteomic screen identified several putative αDystrobrevin-1 interactors recruited to the Y730 site in phosphorylated and unphosphorylated states. Amongst various actin-modulating proteins, we identified the Arp2/3 complex regulator cortactin. We showed that similarly to αDystrobrevin-1, cortactin is strongly enriched at the neuromuscular postsynaptic machinery and obtained results suggesting that these two proteins interact in cell homogenates and at the neuromuscular junctions. Analysis of synaptic morphology in cortactin knockout mice showed abnormalities in the slow-twitching soleus muscle and not in the fast-twitching tibialis anterior. However, muscle strength examination did not reveal apparent deficits in knockout animals.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151409"},"PeriodicalIF":6.6,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000268/pdfft?md5=01b1f23eefb553123bf14cd4b170e3a3&pid=1-s2.0-S0171933524000268-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140346965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells","authors":"Daniel Weidinger ,&nbsp;Julian Jacobsen ,&nbsp;Desiree Alisch ,&nbsp;Hendrik Uebner ,&nbsp;Natalie Heinen ,&nbsp;Lea Greune ,&nbsp;Saskia Westhoven ,&nbsp;Kaschin Jamal Jameel ,&nbsp;Juliane Kronsbein ,&nbsp;Stephanie Pfaender ,&nbsp;Christian Taube ,&nbsp;Sebastian Reuter ,&nbsp;Marcus Peters ,&nbsp;Hanns Hatt ,&nbsp;Jürgen Knobloch","doi":"10.1016/j.ejcb.2024.151408","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151408","url":null,"abstract":"<div><h3>Background</h3><p>Therapeutic options for steroid-resistant non-type 2 inflammation in obstructive lung diseases are limited. Bronchial epithelial cells are key in the pathogenesis by releasing the central proinflammatory cytokine interleukine-8 (IL-8). Olfactory receptors (ORs) are expressed in various cell types. This study examined the drug target potential of ORs by investigating their impact on associated pathophysiological processes in lung epithelial cells.</p></div><div><h3>Methods</h3><p>Experiments were performed in the A549 cell line and in primary human bronchial epithelial cells. OR expression was investigated using RT-PCR, Western blot, and immunocytochemical staining. OR-mediated effects were analyzed by measuring 1) intracellular calcium concentration via calcium imaging, 2) cAMP concentration by luminescence-based assays, 3) wound healing by scratch assays, 4) proliferation by MTS-based assays, 5) cellular vitality by Annexin V/PI-based FACS staining, and 6) the secretion of IL-8 in culture supernatants by ELISA.</p></div><div><h3>Results</h3><p>By screening 100 potential OR agonists, we identified two, Brahmanol and Cinnamaldehyde, that increased intracellular calcium concentrations. The mRNA and proteins of the corresponding receptors OR2AT4 and OR2J3 were detected. Stimulation of OR2J3 with Cinnamaldehyde reduced 1) IL-8 in the absence and presence of bacterial and viral pathogen-associated molecular patterns (PAMPs), 2) proliferation, and 3) wound healing but increased cAMP. In contrast, stimulation of OR2AT4 by Brahmanol increased wound healing but did not affect cAMP and proliferation. Both ORs did not influence cell vitality.</p></div><div><h3>Conclusion</h3><p>ORs might be promising drug target candidates for lung diseases with non-type 2 inflammation. Their stimulation might reduce inflammation or prevent tissue remodeling by promoting wound healing.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151408"},"PeriodicalIF":6.6,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000256/pdfft?md5=e70d3f1f239a419f3746716aef60a9dd&pid=1-s2.0-S0171933524000256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140351823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reorganization of the actin cytoskeleton during the formation of neutrophil extracellular traps (NETs)","authors":"Hans Georg Mannherz ,&nbsp;Heidi Budde ,&nbsp;Muhammad Jarkas ,&nbsp;Roua Hassoun ,&nbsp;Natalia Malek-Chudzik ,&nbsp;Antonina J. Mazur ,&nbsp;Jelena Skuljec ,&nbsp;Refik Pul ,&nbsp;Markus Napirei ,&nbsp;Nazha Hamdani","doi":"10.1016/j.ejcb.2024.151407","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151407","url":null,"abstract":"<div><p>We analyzed actin cytoskeleton alterations during NET extrusion by neutrophil-like dHL-60 cells and human neutrophils in the absence of DNase1 containing serum to avoid chromatin degradation and microfilament disassembly. NET-formation by dHL-60 cells and neutrophils was induced by Ionomycin or phorbol-12-myristat-13-acetate (PMA). Subsequent staining with anti-actin and TRITC-phalloidin showed depolymerization of the cortical F-actin at spatially confined areas, the NET extrusion sites, effected by transient activation of the monooxygenase MICAL-1 supported by the G-actin binding proteins cofilin, profilin, thymosin ß4 and probably the F-actin fragmenting activity of gelsolin and/or its fragments, which also decorated the formed NETs. MICAL-1 itself appeared to be proteolyzed by neutrophil elastase possibly to confine its activity to the NET-extrusion area. The F-actin oxidization activity of MICAL-1 is inhibited by Levosimendan leading to reduced NET-formation. Anti-gasdermin-D immunohistochemistry showed a cytoplasmic distribution in non-stimulated cells. After stimulation the NET-extrusion pore displayed reduced anti-gasdermin-D staining but accumulated underneath the plasma membrane of the remaining cell body. A similar distribution was observed for myosin that concentrated together with cortical F-actin along the periphery of the remaining cell body suggesting force production by acto-myosin interactions supporting NET expulsion as indicated by the inhibitory action of the myosin ATPase inhibitor blebbistatin. Isolated human neutrophils displayed differences in their content of certain cytoskeletal proteins. After stimulation neutrophils with high gelsolin content preferentially formed “cloud”-like NETs, whereas those with low or no gelsolin formed long “filamentous” NETs.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151407"},"PeriodicalIF":6.6,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000244/pdfft?md5=3c9faff4b12ac68c8457db4efad09f59&pid=1-s2.0-S0171933524000244-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140327689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The solute carrier SLC7A1 may act as a protein transporter at the blood-brain barrier","authors":"Magdalena Kurtyka ,&nbsp;Frank Wessely ,&nbsp;Sarah Bau ,&nbsp;Eseoghene Ifie ,&nbsp;Liqun He ,&nbsp;Nienke M. de Wit ,&nbsp;Alberte Bay Villekjær Pedersen ,&nbsp;Maximilian Keller ,&nbsp;Caleb Webber ,&nbsp;Helga E. de Vries ,&nbsp;Olaf Ansorge ,&nbsp;Christer Betsholtz ,&nbsp;Marijke De Bock ,&nbsp;Catarina Chaves ,&nbsp;Birger Brodin ,&nbsp;Morten S. Nielsen ,&nbsp;Winfried Neuhaus ,&nbsp;Robert D. Bell ,&nbsp;Tamás Letoha ,&nbsp;Axel H. Meyer ,&nbsp;Claus U. Pietrzik","doi":"10.1016/j.ejcb.2024.151406","DOIUrl":"10.1016/j.ejcb.2024.151406","url":null,"abstract":"<div><p>Despite extensive research, targeted delivery of substances to the brain still poses a great challenge due to the selectivity of the blood-brain barrier (BBB). Most molecules require either carrier- or receptor-mediated transport systems to reach the central nervous system (CNS). These transport systems form attractive routes for the delivery of therapeutics into the CNS, yet the number of known brain endothelium-enriched receptors allowing the transport of large molecules into the brain is scarce. Therefore, to identify novel BBB targets, we combined transcriptomic analysis of human and murine brain endothelium and performed a complex screening of BBB-enriched genes according to established selection criteria. As a result, we propose the high-affinity cationic amino acid transporter 1 (SLC7A1) as a novel candidate for transport of large molecules across the BBB. Using RNA sequencing and in situ hybridization assays, we demonstrated elevated <em>SLC7A1</em> gene expression in both human and mouse brain endothelium. Moreover, we confirmed SLC7A1 protein expression in brain vasculature of both young and aged mice. To assess the potential of SLC7A1 as a transporter for larger proteins, we performed internalization and transcytosis studies using a radiolabelled or fluorophore-labelled anti-SLC7A1 antibody. Our results showed that SLC7A1 internalised a SLC7A1-specific antibody in human colorectal carcinoma (HCT116) cells. Moreover, transcytosis studies in both immortalised human brain endothelial (hCMEC/D3) cells and primary mouse brain endothelial cells clearly demonstrated that SLC7A1 effectively transported the SLC7A1-specific antibody from luminal to abluminal side. Therefore, here in this study, we present for the first time the SLC7A1 as a novel candidate for transport of larger molecules across the BBB.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151406"},"PeriodicalIF":6.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000232/pdfft?md5=f35f1d0d69bba4a3f28797ca29dc4ad1&pid=1-s2.0-S0171933524000232-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140277743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast","authors":"Kaitlin E. Homa ,&nbsp;Glen M. Hocky ,&nbsp;Cristian Suarez ,&nbsp;David R. Kovar","doi":"10.1016/j.ejcb.2024.151404","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151404","url":null,"abstract":"<div><p>While it is well-established that F-actin networks with specific organizations and dynamics are tightly regulated by distinct sets of associated actin-binding proteins (ABPs), how ABPs self-sort to particular F-actin networks remains largely unclear. We report that actin assembly factors Arp2/3 complex and formin Cdc12 tune the association of ABPs fimbrin Fim1 and tropomyosin Cdc8 to different F-actin networks in fission yeast. Genetic and pharmacological disruption of F-actin networks revealed that Fim1 is preferentially directed to Arp2/3-complex mediated actin patches, whereas Cdc8 is preferentially targeted to formin Cdc12-mediated filaments in the contractile ring. To investigate the role of Arp2/3 complex- and formin Cdc12-mediated actin assembly, we used four-color TIRF microscopy to observe the in vitro reconstitution of ABP sorting with purified proteins. Fim1 or Cdc8 alone bind similarly well to filaments assembled by either assembly factor. However, in ‘competition’ reactions containing both actin assembly factors and both ABPs, ∼2.0-fold more Fim1 and ∼3.5-fold more Cdc8 accumulates on Arp2/3 complex branch points and formin Cdc12-assembled actin filaments, respectively. These findings indicate that F-actin assembly factors Arp2/3 complex and formin Cdc12 help facilitate the recruitment of specific ABPs, thereby tuning ABP sorting and subsequently establishing the identity of F-actin networks in fission yeast.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151404"},"PeriodicalIF":6.6,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000219/pdfft?md5=8304ed8acd8b10fb628417601482cee3&pid=1-s2.0-S0171933524000219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1","authors":"Linnet Bischof,&nbsp;Franziska Schweitzer,&nbsp;Hans-Peter Schmitz,&nbsp;Jürgen J. Heinisch","doi":"10.1016/j.ejcb.2024.151405","DOIUrl":"https://doi.org/10.1016/j.ejcb.2024.151405","url":null,"abstract":"<div><p>Yeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GDP/GTP exchange factor (GEF), remained unaffected. An influence of the voltage-dependent anion channel (VDAC) Por1 on the association of GFP-Rho5 with mitochondria under oxidative stress conditions appeared to be strain-dependent. However, epistasis analyses and bimolecular fluorescence complementation (BiFC) studies indicate a genetic and physical interaction. All four strains lacking a single MOMP were investigated for their effect on mitophagy.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151405"},"PeriodicalIF":6.6,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000220/pdfft?md5=2d55ac6b32853bfa5e71c7b6ab106a18&pid=1-s2.0-S0171933524000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140163207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstitution of cytolinker-mediated crosstalk between actin and vimentin","authors":"Irene Istúriz Petitjean ,&nbsp;Quang D. Tran ,&nbsp;Angeliki Goutou ,&nbsp;Zima Kabir ,&nbsp;Gerhard Wiche ,&nbsp;Cécile Leduc ,&nbsp;Gijsje H. Koenderink","doi":"10.1016/j.ejcb.2024.151403","DOIUrl":"10.1016/j.ejcb.2024.151403","url":null,"abstract":"<div><p>Cell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free reconstitution. By contrast, the interactions between the three cytoskeletal networks are relatively unexplored. They are coupled via crosslinkers of the plakin family such as plectin. These are challenging proteins for reconstitution because of their giant size and multidomain structure. Here we engineer a recombinant actin-vimentin crosslinker protein called ‘ACTIF’ that provides a minimal model system for plectin, recapitulating its modular design with actin-binding and intermediate filament-binding domains separated by a coiled-coil linker for dimerisation. We show by fluorescence and electron microscopy that ACTIF has a high binding affinity for vimentin and actin and creates mixed actin-vimentin bundles. Rheology measurements show that ACTIF-mediated crosslinking strongly stiffens actin-vimentin composites. Finally, we demonstrate the modularity of this approach by creating an ACTIF variant with the intermediate filament binding domain of Adenomatous Polyposis Coli. Our protein engineering approach provides a new cell-free system for the biophysical characterization of intermediate filament-binding crosslinkers and for understanding the mechanical synergy between actin and vimentin in mesenchymal cells.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151403"},"PeriodicalIF":6.6,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000207/pdfft?md5=451d043d0bc0cda6e2659ddfd35dccd1&pid=1-s2.0-S0171933524000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140128663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Actin-membrane linkers: Insights from synthetic reconstituted systems","authors":"Feng-Ching Tsai ,&nbsp;Gwendal Guérin ,&nbsp;Julien Pernier ,&nbsp;Patricia Bassereau","doi":"10.1016/j.ejcb.2024.151402","DOIUrl":"10.1016/j.ejcb.2024.151402","url":null,"abstract":"<div><p>At the cell surface, the actin cytoskeleton and the plasma membrane interact reciprocally in a variety of processes related to the remodeling of the cell surface. The actin cytoskeleton has been known to modulate membrane organization and reshape the membrane. To this end, actin-membrane linking molecules play a major role in regulating actin assembly and spatially direct the interaction between the actin cytoskeleton and the membrane. While studies in cells have provided a wealth of knowledge on the molecular composition and interactions of the actin-membrane interface, the complex molecular interactions make it challenging to elucidate the precise actions of the actin-membrane linkers at the interface. Synthetic reconstituted systems, consisting of model membranes and purified proteins, have been a powerful approach to elucidate how actin-membrane linkers direct actin assembly to drive membrane shape changes. In this review, we will focus only on several actin-membrane linkers that have been studied by using reconstitution systems. We will discuss the design principles of these reconstitution systems and how they have contributed to the understanding of the cellular functions of actin-membrane linkers. Finally, we will provide a perspective on future research directions in understanding the intricate actin-membrane interaction.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151402"},"PeriodicalIF":6.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000190/pdfft?md5=57b937c8a7a0cf9a66db72e1c542da38&pid=1-s2.0-S0171933524000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis","authors":"Yuan Zhuang ,&nbsp;Johanna B. Fischer ,&nbsp;Gopala Nishanth ,&nbsp;Dirk Schlüter","doi":"10.1016/j.ejcb.2024.151401","DOIUrl":"10.1016/j.ejcb.2024.151401","url":null,"abstract":"<div><p>The facultative intracellular bacterium <em>Listeria</em> (<em>L</em>.) <em>monocytogenes</em> may cause severe diseases in humans and animals. The control of listeriosis/<em>L. monocytogenes</em> requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular <em>L. monocytogenes</em>. Both the immune response against <em>L. monocytogenes</em> and cell intrinsic pathogen control are critically regulated by post-translational modifications exerted by the host ubiquitin system and ubiquitin-like modifiers (Ubls). In this review, we discuss our current understanding of the role of the ubiquitin system and Ubls in listeriosis, as well as future directions of research.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 2","pages":"Article 151401"},"PeriodicalIF":6.6,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000189/pdfft?md5=2e8603c47657a372fbad6143dc985edf&pid=1-s2.0-S0171933524000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140007916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}