European journal of cell biology最新文献

{"title":"Salmonella infection impacts host proteome thermal stability","authors":"Marlène S. Birk ,&nbsp;Philipp Walch ,&nbsp;Tarik Baykara ,&nbsp;Stephanie Sefried ,&nbsp;Jan Amelang ,&nbsp;Elena Buerova ,&nbsp;Ingrid Breuer ,&nbsp;Jörg Vervoorts ,&nbsp;Athanasios Typas ,&nbsp;Mikhail M. Savitski ,&nbsp;André Mateus ,&nbsp;Joel Selkrig","doi":"10.1016/j.ejcb.2024.151448","DOIUrl":"10.1016/j.ejcb.2024.151448","url":null,"abstract":"<div><p>Intracellular bacterial pathogens hijack the protein machinery of infected host cells to evade their defenses and cultivate a favorable intracellular niche. The intracellular pathogen <em>Salmonella enterica</em> subsp. Typhimurium (<em>S</em>Tm) achieves this by injecting a cocktail of effector proteins into host cells that modify the activity of target host proteins. Yet, proteome-wide approaches to systematically map changes in host protein function during infection have remained challenging. Here we adapted a functional proteomics approach - Thermal-Proteome Profiling (TPP) - to systematically assess proteome-wide changes in host protein abundance and thermal stability throughout an <em>S</em>Tm infection cycle. By comparing macrophages treated with live or heat-killed <em>S</em>Tm, we observed that most host protein abundance changes occur independently of <em>S</em>Tm viability. In contrast, a large portion of host protein thermal stability changes were specific to infection with live <em>S</em>Tm. This included pronounced thermal stability changes in proteins linked to mitochondrial function (Acod1/Irg1, Cox6c, Samm50, Vdac1, and mitochondrial respiratory chain complex proteins), as well as the interferon-inducible protein with tetratricopeptide repeats, Ifit1. Integration of our TPP data with a publicly available <em>S</em>Tm-host protein-protein interaction database led us to discover that the secreted <em>S</em>Tm effector kinase, SteC, thermally destabilizes and phosphorylates the ribosomal preservation factor Serbp1. In summary, this work emphasizes the utility of measuring protein thermal stability during infection to accelerate the discovery of novel molecular interactions at the host-pathogen interface.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 4","pages":"Article 151448"},"PeriodicalIF":4.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000657/pdfft?md5=0165df0c33e474067e594538a5fed8cd&pid=1-s2.0-S0171933524000657-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of lymph node metastasis: An extracellular vesicle perspective","authors":"Susana García-Silva,&nbsp;Héctor Peinado","doi":"10.1016/j.ejcb.2024.151447","DOIUrl":"10.1016/j.ejcb.2024.151447","url":null,"abstract":"<div><p>In several solid tumors such as breast cancer, prostate cancer, colorectal cancer or melanoma, tumor draining lymph nodes are the earliest tissues where colonization by tumor cells is detected. Lymph nodes act as sentinels of metastatic dissemination, the deadliest phase of tumor progression. Besides hematogenous dissemination, lymphatic spread of tumor cells has been demonstrated, adding more complexity to the mechanisms involved in metastasis. A network of blood and lymphatic vessels surrounds tumors providing routes for tumor soluble factors to mediate regional and long-distance effects. Additionally, extracellular vesicles (EVs), particularly small EVs/exosomes, have been shown to circulate through the blood and lymph, favoring the formation of pre-metastatic niches in the tumor-draining lymph nodes (TDLNs) and distant organs. In this review, we present an overview of the relevance of lymph node metastasis, the structural and immune changes occurring in TDLNs during tumor progression, and how extracellular vesicles contribute to modulating some of these alterations while promoting the formation of lymph node pre-metastatic niches.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151447"},"PeriodicalIF":4.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000645/pdfft?md5=ea2eecc58a41812b39eed80a2c9b37b9&pid=1-s2.0-S0171933524000645-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elimination of the extra chromosome of Dup15q syndrome iPSCs for cellular and molecular investigation","authors":"Haruka Munezane ,&nbsp;Keiko Imamura ,&nbsp;Naoko Fujimoto ,&nbsp;Akitsu Hotta ,&nbsp;Hiroshi Yukitake ,&nbsp;Haruhisa Inoue","doi":"10.1016/j.ejcb.2024.151446","DOIUrl":"10.1016/j.ejcb.2024.151446","url":null,"abstract":"<div><p>Chromosome 15q11.2–13.1 duplication (Dup15q) syndrome is one of the most common autism spectrum disorders (ASDs) associated with copy number variants (CNVs). For the analysis of CNV-relevant pathological cellular phenotypes, a CNV-corrected isogenic cell line is useful for excluding the influence of genetic background. Here, we devised a strategy to remove the isodicentric chromosome 15 by inserting a puro-ΔTK selection cassette into the extra chromosome using the CRISPR-Cas9 system, followed by a subsequent two-step drug selection. A series of assays, including qPCR-based copy number analysis and karyotype analysis, confirmed the elimination of the extra chromosome. Furthermore, cerebral organoids were generated from the parental Dup15q iPSCs and their isogenic iPSCs. scRNA-seq analysis revealed the alteration of expression levels in ion-channel-related genes and synapse-related genes in glutamatergic and GABAergic neurons in Dup15q organoids, respectively. The established isogenic cell line is a valuable resource for unraveling cellular and molecular alterations associated with Dup15q syndrome.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151446"},"PeriodicalIF":4.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000633/pdfft?md5=544af3c06a8f5da20823c813cbf3db8d&pid=1-s2.0-S0171933524000633-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression profiles of piRNAs and their interacting Piwi proteins in cellular model of renal development: Focus on Piwil1 in mitosis","authors":"Marek Kazimierczyk,&nbsp;Agnieszka Fedoruk-Wyszomirska,&nbsp;Dorota Gurda-Woźna,&nbsp;Eliza Wyszko,&nbsp;Agata Swiatkowska,&nbsp;Jan Wrzesinski","doi":"10.1016/j.ejcb.2024.151444","DOIUrl":"10.1016/j.ejcb.2024.151444","url":null,"abstract":"<div><p>Piwi proteins and Piwi interacting RNAs, piRNAs, presented in germline cells play a role in transposon silencing during germline development. In contrast, the role of somatic Piwi proteins and piRNAs still remains obscure. Here, we characterize the expression pattern and distribution of piRNAs in human renal cells in terms of their potential role in kidney development. Further, we show that all <em>PIWI</em> genes are expressed at the RNA level, however, only <em>PIWIL1</em> gene is detected at the protein level by western blotting in healthy and cancerous renal cells. So far, the expression of human Piwil1 protein has only been shown in testes and cancer cells, but not in healthy somatic cell lines. Since we observe only Piwil1 protein, the regulation of other <em>PIWI</em> genes is probably more intricated, and depends on environmental conditions. Next, we demonstrate that downregulation of Piwil1 protein results in a decrease in the rate of cell proliferation, while no change in the level of apoptotic cells is observed. Confocal microscopy analysis reveals that Piwil1 protein is located in both cellular compartments, cytoplasm and nucleus in renal cells. Interestingly, in nucleus region Piwil1 is observed close to the spindle during all phases of mitosis in all tested cell lines. It strongly indicates that Piwil1 protein plays an essential role in proliferation of somatic cells. Moreover, involvement of Piwil1 in cell division could, at least partly, explain invasion and metastasis of many types of cancer cells with upregulation of <em>PIWIL1</em> gene expression. It also makes Piwil1 protein as a potential target in the anticancer therapy.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151444"},"PeriodicalIF":4.5,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S017193352400061X/pdfft?md5=7451054a08e402ce6ec1ba62765123ed&pid=1-s2.0-S017193352400061X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xenogeneic versus allogeneic serum and macromolecular crowding in human tenocyte cultures","authors":"Andrea Rampin ,&nbsp;Andrea Rossoni ,&nbsp;Lefki Chaniotaki ,&nbsp;Ioannis S. Gkiatas ,&nbsp;Athina Tzora ,&nbsp;Ioannis Skoufos ,&nbsp;Nikolaos Diakakis ,&nbsp;Nikitas Prassinos ,&nbsp;Dimitrios I. Zeugolis","doi":"10.1016/j.ejcb.2024.151445","DOIUrl":"10.1016/j.ejcb.2024.151445","url":null,"abstract":"<div><p>Allogeneic serum and tissue-specific extracellular matrix have been shown to maintain permanently differentiated cell phenotype in culture. This is of particular importance for human tenocytes, a cell population that readily loses its function during ex vivo culture. With these in mind, herein we extracted human tenocytes using either foetal bovine serum or human serum, cultured them in the absence and presence of carrageenan and Ficoll®, the most widely used macromolecular crowding agents (to induce tissue-specific extracellular matrix deposition), and assessed cellular function, via metabolic activity, viability, proliferation and immunofluorescence for collagen related molecules, non-collagenous molecules and transmembrane molecules. At day 7, longest time point assessed, neither carrageenan nor Ficoll® significantly affected metabolic activity, viability and proliferation in either serum and human serum significantly increased metabolic activity and proliferation. At day 7, in the absence of macromolecular crowding, cells in human serum deposited significantly lower collagen type VI, biglycan, versican and tenomodulin than cells in foetal bovine serum. Interestingly, at day 7, in comparison to the no macromolecular crowding group, carrageenan in foetal bovine serum induced the highest effect, as judged by the highest number of significantly increased molecules (collagen type I, collagen type IV, collagen type V, collagen type VI, transforming growth factor β1, matrix metalloproteinase 14, lumican, versican, scleraxis and integrin α2β1). These data, although contradict previous observations where human serum outperformed foetal bovine serum, at the same time, support the use of foetal bovine serum in the development of cell-based medicines.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151445"},"PeriodicalIF":4.5,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000621/pdfft?md5=a1fdf9af0e9457eb6c97bd2d3faff8f2&pid=1-s2.0-S0171933524000621-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial - In vitro reconstitution of cytoskeletal processes","authors":"Antoine Jégou,&nbsp;Guillaume Romet-Lemonne","doi":"10.1016/j.ejcb.2024.151443","DOIUrl":"10.1016/j.ejcb.2024.151443","url":null,"abstract":"","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151443"},"PeriodicalIF":4.5,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000608/pdfft?md5=14d27deaa175562ed1b5c6c31fce5b2d&pid=1-s2.0-S0171933524000608-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141699712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine-derived renal epithelial cells isolated after kidney transplant are sensitive to neutrophil gelatinase-associated lipocalin exposure during in vitro culture","authors":"Valeria Pizzuti ,&nbsp;Emma Balducelli ,&nbsp;Miriam Di Nunzio ,&nbsp;Diletta Conte ,&nbsp;Elisa Gessaroli ,&nbsp;Marcello Demetri ,&nbsp;Pasquale Marrazzo ,&nbsp;Francesco Alviano ,&nbsp;Valeria Corradetti ,&nbsp;Federica Maritati ,&nbsp;Gaetano La Manna ,&nbsp;Giorgia Comai","doi":"10.1016/j.ejcb.2024.151442","DOIUrl":"10.1016/j.ejcb.2024.151442","url":null,"abstract":"<div><p>Urine-derived renal epithelial cells (URECs) are highly voided after kidney transplant and express typical kidney markers, including markers of kidney epithelial progenitor cells. Recently URECs have shown promising immunomodulatory properties when cultured with Peripheral Blood Mononuclear Cells (PBMCs), promoting an increase in the T regulatory cells. In vivo, kidney cells are highly exposed to damage associated molecules during both acute and chronic kidney injury. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most -known early marker of acute and chronic kidney damage. However, its role on the evolution of renal damage has not yet been fully described, nor has its impact on the characteristics of renal-derived cells during in vitro culture. The aim of this study is to investigate the effect of NGAL on the characteristics of URECs isolated after kidney transplant, by exposing these cells to the treatment with NGAL during in vitro culture and evaluating its effect on UREC viability, proliferation, and immunomodulatory potential. The exposure of URECs to NGAL reduced their viability and proliferative capacity, promoting the onset of apoptosis. The immunomodulatory properties of URECs were partially inhibited by NGAL, without affecting the increase of Treg cells observed during UREC-PBMCs coculture. These results suggest that the exposure to NGAL may compromise some features of kidney stem and specialized cell types, reducing their viability, increasing apoptosis, and partially altering their immunomodulatory properties. Thus, NGAL could represent a target for approaches acting on its inhibition or reduction to improve functional recovery.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151442"},"PeriodicalIF":4.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000591/pdfft?md5=3bf86adbdd800b05eb371569a08ce6ca&pid=1-s2.0-S0171933524000591-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of integrins in brain health and neurodegenerative diseases","authors":"Yunhao Cui ,&nbsp;Taisia Rolova ,&nbsp;Susanna C. Fagerholm","doi":"10.1016/j.ejcb.2024.151441","DOIUrl":"10.1016/j.ejcb.2024.151441","url":null,"abstract":"<div><p>Integrins are heterodimeric membrane proteins expressed on the surface of most cells. They mediate adhesion and signaling processes relevant for a wealth of physiological processes, including nervous system development and function. Interestingly, integrins are also recognized therapeutic targets for inflammatory diseases, such as multiple sclerosis. Here, we discuss the role of integrins in brain development and function, as well as in neurodegenerative diseases affecting the brain (Alzheimer’s disease, multiple sclerosis, stroke). Furthermore, we discuss therapeutic targeting of these adhesion receptors in inflammatory diseases of the brain.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151441"},"PeriodicalIF":4.5,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S017193352400058X/pdfft?md5=f293ae17864918e41908a51a93cbe440&pid=1-s2.0-S017193352400058X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinase activity of histone chaperone APLF maintains steady state of centrosomes in mouse embryonic stem cells","authors":"Sruthy Manuraj Rajam ,&nbsp;Pallavi Chinnu Varghese ,&nbsp;Mayur Balkrishna Shirude ,&nbsp;Khaja Mohieddin Syed ,&nbsp;Anjali Devarajan ,&nbsp;Kathiresan Natarajan ,&nbsp;Debasree Dutta","doi":"10.1016/j.ejcb.2024.151439","DOIUrl":"10.1016/j.ejcb.2024.151439","url":null,"abstract":"<div><p>Our recent studies revealed the role of mouse Aprataxin PNK-like Factor (APLF) in development. Nevertheless, the comprehensive characterization of mouse APLF remains entirely unexplored. Based on domain deletion studies, here we report that mouse APLF's Acidic Domain and Fork Head Associated (FHA) domain can chaperone histones and repair DNA like the respective human orthologs. Immunofluorescence studies in mouse embryonic stem cells showed APLF co-localized with γ-tubulin within and around the centrosomes and govern the number and integrity of centrosomes via PLK4 phosphorylation. Enzymatic analysis established mouse APLF as a kinase. Docking studies identified three putative ATP binding sites within the FHA domain. Site-directed mutagenesis showed that R37 residue within the FHA domain is indispensable for the kinase activity of APLF thereby regulating the centrosome number. These findings might assist us comprehend APLF in different pathological and developmental conditions and reveal non-canonical kinase activity of proteins harbouring FHA domains that might impact multiple cellular processes.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151439"},"PeriodicalIF":4.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000566/pdfft?md5=f9b67a2e63edf7473ac1ba067f0da878&pid=1-s2.0-S0171933524000566-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations of receptors and insulin-like growth factor binding proteins in senescent cells","authors":"Julia Matuszewska ,&nbsp;Adrianna Krawiec ,&nbsp;Artur Radziemski ,&nbsp;Paweł Uruski ,&nbsp;Andrzej Tykarski ,&nbsp;Justyna Mikuła-Pietrasik ,&nbsp;Krzysztof Książek","doi":"10.1016/j.ejcb.2024.151438","DOIUrl":"10.1016/j.ejcb.2024.151438","url":null,"abstract":"<div><p>The knowledge about cellular senescence expands dynamically, providing more and more conclusive evidence of its triggers, mechanisms, and consequences. Senescence-associated secretory phenotype (SASP), one of the most important functional traits of senescent cells, is responsible for a large extent of their context-dependent activity. Both SASP’s components and signaling pathways are well-defined. A literature review shows, however, that a relatively underinvestigated aspect of senescent cell autocrine and paracrine activity is the change in the production of proteins responsible for the reception and transmission of SASP signals, i.e., receptors and binding proteins. For this reason, we present in this article the current state of knowledge regarding senescence-associated changes in cellular receptors and insulin-like growth factor binding proteins. We also discuss the role of these alterations in senescence induction and maintenance, pro-cancerogenic effects of senescent cells, and aging-related structural and functional malfunctions.</p></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"103 3","pages":"Article 151438"},"PeriodicalIF":4.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171933524000554/pdfft?md5=686a49fea7150fa4545825733a880e8a&pid=1-s2.0-S0171933524000554-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}