European journal of cell biology最新文献_第4页

A diverse interactome of the molecular chaperone CCT/TRiC monomers 分子伴侣CCT/TRiC单体的多种相互作用

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-09-01 Epub Date: 2025-07-09 DOI: 10.1016/j.ejcb.2025.151506

Carmen M. Córdoba-Beldad, Julie Grantham

引用次数: 0

Interference of estrogen signaling by endocrine disruptors in male and female cells: Potential implications of BPS and PFOS in human development 男性和女性细胞中内分泌干扰物对雌激素信号的干扰：BPS和PFOS在人类发育中的潜在影响

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-09-01 Epub Date: 2025-07-20 DOI: 10.1016/j.ejcb.2025.151509

Giulia Gaggi , Andrea Di Credico , Sandra Bibbò , Barbara Corneo , Alberto Ferlin , Angela Di Baldassarre , Barbara Ghinassi

{"title":"Interference of estrogen signaling by endocrine disruptors in male and female cells: Potential implications of BPS and PFOS in human development","authors":"Giulia Gaggi , Andrea Di Credico , Sandra Bibbò , Barbara Corneo , Alberto Ferlin , Angela Di Baldassarre , Barbara Ghinassi","doi":"10.1016/j.ejcb.2025.151509","DOIUrl":"10.1016/j.ejcb.2025.151509","url":null,"abstract":"<div><div>Endocrine-disruptors (EDs), such as bisphenol-S (BPS) and perfluorooctane-sulfonate (PFOS), can cross the placental barrier and interfere with fetal development, inducing consequences that seem to be more pronounced in males. We investigated whether BPS and PFOS could have different effects on male and female human induced-pluripotent-stem cells (hiPSCs) by analyzing their impact on estrogen signaling pathways. Our results demonstrate that in male hiPSCs, BPS and PFOS induce alterations in the estrogen pathways, confirming their role as xenoestrogens. BPS and PFOS also upregulated oxidative phosphorylation proteins in males, while disruptions in Golgi apparatus integrity were observed in female cells. These findings highlight the differential susceptibility of male and female cells to ED exposure and suggest that such chemicals, perturbing the hormonal network, may affect developmental programming and long-term health. Moreover, this study emphasizes the importance of considering sex-specific responses to environmental pollutants and their impact during the highly sensitive periods of fetal growth.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 3","pages":"Article 151509"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity and function of auxiliary fluxes of glucose metabolism in response to physiological normoxia (5 % O2) during long-term Adipose-Derived Stem/Stromal cell culture 长期脂肪源性干细胞/基质细胞培养过程中生理常氧（5 % O2）下葡萄糖代谢辅助通量的活性和功能

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-03-28 DOI: 10.1016/j.ejcb.2025.151486

Paulina Rybkowska , Maria Kawalec , Dorota Dymkowska , Klaudia Radoszkiewicz , Barbara Zabłocka , Krzysztof Zabłocki , Anna Sarnowska

{"title":"Activity and function of auxiliary fluxes of glucose metabolism in response to physiological normoxia (5 % O2) during long-term Adipose-Derived Stem/Stromal cell culture","authors":"Paulina Rybkowska , Maria Kawalec , Dorota Dymkowska , Klaudia Radoszkiewicz , Barbara Zabłocka , Krzysztof Zabłocki , Anna Sarnowska","doi":"10.1016/j.ejcb.2025.151486","DOIUrl":"10.1016/j.ejcb.2025.151486","url":null,"abstract":"<div><div>Energy metabolism homeostasis emerges as a dominant element influencing mesenchymal stem/stromal cells’ trajectory of development. The predominant glycolysis activity is a primary driver of cell proliferation and maintenance of the high-energetic state. Here, we examined the functions of two crucial auxiliary pathways: the phosphate-pentose pathway (PPP) and fructose-2,6-biphosphate pathway (FBP) to evaluate their impact on the therapeutic potential of Adipose-Derived Stem/Stromal cells (ASCs) during prolonged culture in various oxygen conditions: 5 % O<sub>2</sub> - physiological normoxia or 21 % O<sub>2</sub> - atmospheric oxygen. Our findings demonstrate that ASCs cultured in 5 % O<sub>2</sub> increased the rate of proliferation, migration, and expression of stemness factors, which is prominent during the initial and middle passages. Additionally, ASCs cultured in a 5 % O<sub>2</sub> exhibited heightened protection mechanisms against free radicals, increased LDH gene expression, and elevated extracellular acidification rate (ECAR). By estimating the HIF-1α level, we concluded that 5 % oxygen conditions were insufficient to induce a profound hypoxic state in ASCs. However, at the protein level, both the PPP and FBP pathways appeared to be more active in young (2-passage) cells, regardless of oxygen conditions, and their activity diminished over time. Additionally, the chemical suppression of G6PDH by Polydatin and inhibition of PFKFB3 by PFK-158 in ASCs (passage-2) revealed dose- and time-dependent effect on decreasing migratory capabilities of cells. Nevertheless, our work underscores the adaptable nature of ASC metabolism to prevailing external conditions, with the aging of the culture contributing to the decline in glycolysis-associated auxiliary pathways.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151486"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Mouse colorectal cancer organoids: Lessons from syngeneic and orthotopic transplantation systems 小鼠结直肠癌类器官：同基因和原位移植系统的经验教训

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-02-06 DOI: 10.1016/j.ejcb.2025.151478

Yu Muta, Yuki Nakanishi

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iPSC screening identifies CACNA2D2 as a potential therapeutic target for FTLD-Tau iPSC筛选发现CACNA2D2是FTLD-Tau的潜在治疗靶点

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-03-21 DOI: 10.1016/j.ejcb.2025.151484

Keiko Imamura , Ayako Nagahashi , Aya Okusa , Tomoki Sakasai , Kayoko Tsukita , Yumiko Kutoku , Yutaka Ohsawa , Yoshihide Sunada , Naruhiko Sahara , Nicholas M. Kanaan , Makoto Higuchi , Kohji Mori , Manabu Ikeda , Haruhisa Inoue

{"title":"iPSC screening identifies CACNA2D2 as a potential therapeutic target for FTLD-Tau","authors":"Keiko Imamura , Ayako Nagahashi , Aya Okusa , Tomoki Sakasai , Kayoko Tsukita , Yumiko Kutoku , Yutaka Ohsawa , Yoshihide Sunada , Naruhiko Sahara , Nicholas M. Kanaan , Makoto Higuchi , Kohji Mori , Manabu Ikeda , Haruhisa Inoue","doi":"10.1016/j.ejcb.2025.151484","DOIUrl":"10.1016/j.ejcb.2025.151484","url":null,"abstract":"<div><div>Frontotemporal Lobar Degeneration (FTLD) is a neurodegenerative disorder that affects the frontal and temporal lobes, which are crucial for regulating personality, behavior, and language. Pathologically, FTLD is characterized by Tau protein accumulation and neuronal death. In our effort to identify disease-modifying treatments, we conducted drug screening using neurons derived from induced pluripotent stem cells (iPSCs) of FTLD-Tau patients. This screening identified gabapentin as an existing drug that suppresses neuronal cell death with suppressed accumulation of Tau oligomers. Treatment with gabapentinoids, including pregabalin and mirogabalin, demonstrated similar neuroprotective effects. These compounds bind to the α2δ subunit of voltage-dependent calcium channels and specifically target the two isoforms α2δ-1 and α2δ-2. To determine which isoform is involved in the neurodegeneration seen in FTLD-Tau, we employed a knockout approach using iPSCs, which revealed that α2δ-2, encoded by CACNA2D2, plays a key role in the degeneration of FTLD-Tau neurons. Moreover, Neural organoids of FTLD-Tau exhibited features indicative of neurodegeneration, and CACNA2D2 knockout reversed a part of the gene expression alterations associated with these neurodegenerative features. These findings suggest that α2δ-2 may be a promising target for disease-modifying therapies in FTLD-Tau.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151484"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Air-liquid interface culture alters the characteristics and functions of monolayers generated from human iPS cell‑derived enterocyte‑like cell organoids 气液界面培养改变了人类iPS细胞衍生的肠细胞样细胞类器官产生的单层膜的特性和功能

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-02-05 DOI: 10.1016/j.ejcb.2025.151479

Tatsuya Inui , Yusei Uraya , Yukiko Ueyama-Toba , Hiroyuki Mizuguchi

{"title":"Air-liquid interface culture alters the characteristics and functions of monolayers generated from human iPS cell‑derived enterocyte‑like cell organoids","authors":"Tatsuya Inui , Yusei Uraya , Yukiko Ueyama-Toba , Hiroyuki Mizuguchi","doi":"10.1016/j.ejcb.2025.151479","DOIUrl":"10.1016/j.ejcb.2025.151479","url":null,"abstract":"<div><div>To evaluate the intestinal absorption and metabolism of orally administered drugs, human induced pluripotent stem (iPS) cell‑derived enterocyte‑like cells (ELCs) are expected to be useful. In a previous report, we succeeded in developing a highly functional monolayer platform (ELC-org-mono) from human iPS cell-derived ELCs through an organoid culture and demonstrated its suitability for pharmacokinetic studies. In recent years, the air–liquid interface (ALI) culture model was developed, allowing for the culture of epithelial tissue under conditions that mimic the in vivo environment. In the present study, we applied ALI culture to ELC-org-mono for further improvement of intestinal functions. ALI culture of ELC-org-mono greatly developed goblet cells and enhanced the gene expression levels of many drug-metabolizing enzymes, drug transporters and intestinal differentiation markers. However, their activities were not enhanced. RNA-seq analysis suggested that ALI culture increased the expression of genes related to metabolic processes but decreased glycolytic processes. Analysis of glycolytic capacity confirmed that ALI culture decreased glycolytic activities. Thus, there is room for some adjustment in the ALI culture model to optimize its applicability to pharmacokinetic studies using ELC-org-mono.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151479"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143347848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress Vitronectin调节人胎盘源间充质干细胞在营养胁迫下的黏附转换和迁移。

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-01-27 DOI: 10.1016/j.ejcb.2025.151477

Srishti Dutta Gupta, Nitish Pal, Malancha Ta

{"title":"Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress","authors":"Srishti Dutta Gupta, Nitish Pal, Malancha Ta","doi":"10.1016/j.ejcb.2025.151477","DOIUrl":"10.1016/j.ejcb.2025.151477","url":null,"abstract":"<div><div>At sites of tissue damage and wound healing, the mesenchymal stem cells (MSCs) are often challenged by nutrient availability due to blood supply disruption. Thus, it becomes critical to identify novel factors and their mechanism of action in regulating the adhesion and migration of MSCs under nutrient stress condition for successful clinical application. In human placenta-derived MSCs (PL-MSCs), we demonstrated an increase in cell spread area, along with increased adhesion and reduced migration of the cells, when cultured under nutrient stress condition. Correspondingly, an increase in the total number per cell and size of focal adhesions (FAs), together with prominent stress fibers were observed in nutrient-stressed PL-MSCs compared to control PL-MSCs. The FAs were demonstrated to be more stable, exhibiting slower turnover and longer lifespan. Vitronectin (VTN), an ECM glycoprotein, was upregulated under nutrient stress condition. Knockdown of VTN in PL-MSCs led to a significant reduction in the total number per cell and size of FAs, along with their faster turnover and shorter lifespan. Subsequently, a reversal in the cell spread area, adhesion and migration properties of the nutrient-stressed PL-MSCs were noted. Additionally, our findings indicated that VTN, as an upstream regulator, stimulated the phosphorylation of myosin light chain, which possibly promoted the maturation and stability of FAs along with assembly of stress fibers, thereby leading to increased adhesion and reduced migration of the cells. Overall, our study defines a distinct role of VTN as a critical regulator of migration in PL-MSCs under nutrient stress condition.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151477"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of organoid genetics 类器官遗传学的进化

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-02-28 DOI: 10.1016/j.ejcb.2025.151481

Thomas M. Klompstra , Ki-Jun Yoon , Bon-Kyoung Koo

{"title":"Evolution of organoid genetics","authors":"Thomas M. Klompstra , Ki-Jun Yoon , Bon-Kyoung Koo","doi":"10.1016/j.ejcb.2025.151481","DOIUrl":"10.1016/j.ejcb.2025.151481","url":null,"abstract":"<div><div>Organoids have revolutionized <em>in vitro</em> research by offering three-dimensional, multicellular systems that recapitulate the structure, function, and genetics of human tissues. Initially developed from both pluripotent stem cells (PSCs) and adult stem cells (AdSCs), organoids have expanded to model nearly every major human organ, significantly advancing developmental biology, disease modeling, and therapeutic screening. This review highlights the progression of organoid technologies, emphasizing the integration of genetic tools, including CRISPR-Cas9, prime editing, and lineage tracing. These advancements have facilitated precise modeling of human-specific pathologies and drug responses, often surpassing traditional 2D cultures and animal models in accuracy. Emerging technologies, such as organoid fusion, xenografting, and optogenetics, are expected to further enhance our understanding of cellular interactions and microenvironmental dynamics. As organoid complexity and genetic engineering methods continue to evolve, they will become increasingly indispensable for personalized medicine and translational research, bridging gaps between <em>in vitro</em> and <em>in vivo</em> systems.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151481"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral vaccines promote endoplasmic reticulum stress-induced unfolding protein response in teleost erythrocytes 病毒疫苗促进硬化红细胞内质网应激诱导的展开蛋白反应

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-04-09 DOI: 10.1016/j.ejcb.2025.151490

Maria Salvador-Mira , Paula Gimenez-Moya , Alba Manso-Aznar , Ester Sánchez-Córdoba , Manuel A. Sevilla-Diez , Veronica Chico , Ivan Nombela , Sara Puente-Marin , Nerea Roher , Luis Perez , Tanja Dučić , Núria Benseny-Cases , Ana Joaquina Perez-Berna , Maria del Mar Ortega-Villaizan

{"title":"Viral vaccines promote endoplasmic reticulum stress-induced unfolding protein response in teleost erythrocytes","authors":"Maria Salvador-Mira , Paula Gimenez-Moya , Alba Manso-Aznar , Ester Sánchez-Córdoba , Manuel A. Sevilla-Diez , Veronica Chico , Ivan Nombela , Sara Puente-Marin , Nerea Roher , Luis Perez , Tanja Dučić , Núria Benseny-Cases , Ana Joaquina Perez-Berna , Maria del Mar Ortega-Villaizan","doi":"10.1016/j.ejcb.2025.151490","DOIUrl":"10.1016/j.ejcb.2025.151490","url":null,"abstract":"<div><div>Most available evidence points to a proviral role for endoplasmic reticulum (ER) stress, as many viruses exploit it to promote viral replication. In contrast, few studies have linked ER stress to the antiviral immune response, and even fewer to the vaccine-induced immune response. In this work, we demonstrated that ER stress is a key molecular link in the immune response of teleost erythrocytes or red blood cells (RBCs) under vaccine stimulation. Moreover, the unfolded protein response (UPR<sup>ER</sup>) triggered by ER stress may work together with autophagy and related cellular mechanisms as part of a coordinated immune response in RBCs. We unveiled biochemical changes in the lipid-protein profile of vaccine-treated RBCs by synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-µFTIR) associated with the modulation of ER expansion, increased mitochondrial number, and vesicular structures detected by soft X-ray cryotomography (cryo-SXT). We found a positive correlation between both morphological and biochemical changes and the expression of genes related to UPR<sup>ER</sup>, autophagy, mitochondrial stress, vesicle trafficking, and extracellular vesicle release. These processes in RBCs are ideal cellular targets for the development of more specific prophylactic tools with greater immunogenic capacity than currently available options.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151490"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization 全息层析显微镜显示内皮细胞在内皮化过程中无标记的定量动力学

IF 4.5 3区生物学

European journal of cell biology Pub Date : 2025-06-01 Epub Date: 2025-04-22 DOI: 10.1016/j.ejcb.2025.151492

William D. Leineweber , Gabriela Acevedo Munares , Christian Leycam , Raul Michael , Juliette Noyer , Patrick Jurney

{"title":"Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization","authors":"William D. Leineweber , Gabriela Acevedo Munares , Christian Leycam , Raul Michael , Juliette Noyer , Patrick Jurney","doi":"10.1016/j.ejcb.2025.151492","DOIUrl":"10.1016/j.ejcb.2025.151492","url":null,"abstract":"<div><div>Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization. The study highlights how HTM on a commercial instrument can uncover novel insights into HUVEC cell behavior, offering potential applications in medical diagnostics and research, particularly in developing treatments for cardiovascular diseases. This advanced imaging technique not only enhances our understanding of EC biology but also presents a significant step forward in the study of cardiovascular diseases, providing a robust platform for future research and therapeutic development.</div></div>","PeriodicalId":12010,"journal":{"name":"European journal of cell biology","volume":"104 2","pages":"Article 151492"},"PeriodicalIF":4.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0