EUREKA: Life Sciences最新文献_第4页

Dengue fever in Nigeria: a mini review 尼日利亚的登革热:一个小综述

EUREKA: Life Sciences Pub Date : 2023-05-15 DOI: 10.21303/2504-5695.2023.002906

Daniel Thakuma Tizhe, Dentsen Fortune Dashe, J. Kwaga

{"title":"Dengue fever in Nigeria: a mini review","authors":"Daniel Thakuma Tizhe, Dentsen Fortune Dashe, J. Kwaga","doi":"10.21303/2504-5695.2023.002906","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002906","url":null,"abstract":"Dengue is a disease caused by dengue virus. It is a major mosquito-borne viral disease of humans that is endemic in areas of tropical and subtropical regions, which are environmentally suitable for vector propagation. The disease poses a major threat to the overwhelmed and weak public healthcare delivery system in the developing world, especially in Africa where febrile illnesses are common. The disease undermines the fight against febrile illnesses as infection with dengue often remains undetected or misdiagnosed as malaria or other febrile diseases. This review article highlights dengue epidemiology in Nigeria, dengue transmission dynamics, pathogenesis, diagnosis and current interventions strategies, challenges in addressing dengue infection and future prospects towards dengue elimination. The literature search for publications on dengue virus infection in Nigeria was performed using Google Scholar, PubMed, Web of Science, African Journals Online and other scholarly online databases. Dengue remains a threat to Nigeria and other African countries. In Nigeria, the occurrence of this infection remains a low priority in the public health sector even though it has been reported in about 17 states across the country. The reports of dengue infection in all the geo-political zones suggests active transmission of dengue, hence the need to consider other etiologies of febrile illnesses and engage the public to sustain local involvement as local risk to dengue outbreaks is linked to the population’s knowledge, attitude and behavioral practices that encourage vector breeding","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72600716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary schistosomiasis: risk factors and symptoms among school adolescents in Kaduna State, Nigeria 尿路血吸虫病:尼日利亚卡杜纳州在校青少年的危险因素和症状

EUREKA: Life Sciences Pub Date : 2023-05-15 DOI: 10.21303/2504-5695.2023.002905

H. Bishop, H. Inabo, Elijah Ekah Ella, Mohammed Bello

{"title":"Urinary schistosomiasis: risk factors and symptoms among school adolescents in Kaduna State, Nigeria","authors":"H. Bishop, H. Inabo, Elijah Ekah Ella, Mohammed Bello","doi":"10.21303/2504-5695.2023.002905","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002905","url":null,"abstract":"Improper waste disposal, unsafe water and indiscriminate water-contact activities are major factors enhancing continuous spread of schistosomiasis in Nigeria. Many water bodies are prone to contamination with human wastes directly discharged into them or due to surface runoff, and are infested with parasites. Open defecation and discharge of household sewage into water channels is still practiced. Children conduct activities in these water bodies, thereby exposing themselves to infections with schistomes among other pathogens. Urine samples (10 mL each) were collected from 600 consented school adolescents across six Local Government Areas of Kaduna State, Nigeria. Information on their water-contact activities were obtained by means of questionnaires. Urine sediment was examined for Schistosoma haematobium eggs by microscopy. No infection was recorded in adolescents who had awareness about the disease. Those who engaged in swimming (9.2 %, OR=2.2) and fishing (10.3 %, OR=2.1) were significantly more infected than those who did not (P≤0.05). Adolescents who worked on irrigated farms (9.0 %, OR=1.4), washed clothes in rivers (9.0 %, OR=1.6), or fetch water from rivers for domestic purpose (10.0 %) were more infected than others who did not engage in those activities. Therefore, swimming and fishing are important factors enhancing the spread of schistosomiasis among school adolescents in Kaduna State. Irrigation farming, washing of clothes in rivers or fetching water from rivers exposed the adolescents to schistosome infections. Widespread awareness campaigns, provision safe water to communities, and standard water-based recreational centers are paramount","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75096246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal assessment of pupal habitat productivity of malaria vector: Anopheles gambiae s.l as influence by physico-chemical conditions at selected breeding habitats in Niger, Nigeria 在尼日利亚尼日尔选定的繁殖生境中，疟疾病媒冈比亚按蚊蛹生境生产力受物化条件影响的季节性评估

EUREKA: Life Sciences Pub Date : 2023-05-15 DOI: 10.21303/2504-5695.2023.002841

Shitta Kefas Babale, Hasber Salim, I. Yakudima, Buda Mohammed Kabir, Rashidu Mamman, Usman Mohammed Chiroma, Shehu Kura Ibrahim

{"title":"Seasonal assessment of pupal habitat productivity of malaria vector: Anopheles gambiae s.l as influence by physico-chemical conditions at selected breeding habitats in Niger, Nigeria","authors":"Shitta Kefas Babale, Hasber Salim, I. Yakudima, Buda Mohammed Kabir, Rashidu Mamman, Usman Mohammed Chiroma, Shehu Kura Ibrahim","doi":"10.21303/2504-5695.2023.002841","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002841","url":null,"abstract":"Malaria is the most significant protozoan disease in Africa and the principal vector-borne disease (VBD) in Nigeria, which is influenced by the quality of breeding habitats that are reflected through the stage preceding adult. Control of Anopheles gambiae s.l. populations through source reduction is still considered the most effective way of prevention and control, although it has proven unsustainable in Niger State. Physico-chemical cues were investigated. Samples were taken inside and outside 1m2 cages weekly by dipping and emptying the cages from May 2019 to March 2020. The data subjected to analysis of variance (one and two-way Anova). The findings revealed the mean pupae abundance (MPA), were significantly higher in Large Water bodies (624.50±217.81), and followed by Gutters (436.00±184.2) and Swamps (285.50±125.06). The mean pupae productivity (MPP), followed the order of descending rate >GT (717.50±219.38) >LW (677.21±145.10) >SW (530.40±136.97). The result also showed that emptying technique (ET) was more sufficient and reliable than dipping technique (DT). The peak abundance and productivity of the pupal stage was June to August then declined in March,2020 both habitats. However, MPP differed significantly (p<0.05) from one another across the months in all the habitat types. The physical and chemical cues of the breeding sites, varied significantly, except in temperature, total hardness, biochemical oxygen demands, conductivity, and pH in all the habitats. This study revealed high utilization of physico-chemical properties and poses increased risk of malaria. Thus, emphasis on the vector management strategies should be given specially on gutters and large water bodies as breeding habitats of malaria vectors (MV), in Niger State","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88990502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplement using cockroach meal: impact on growth indices and biochemical responses in hybrid catfish 饲料中添加蟑螂粉对杂交鲶鱼生长指标和生化反应的影响

EUREKA: Life Sciences Pub Date : 2023-05-15 DOI: 10.21303/2504-5695.2023.002909

A. O. Kolawole, K. Adamu, I. Usman, Yakubu Manbe Mohammed, M. Aliyu-Paiko

{"title":"Dietary supplement using cockroach meal: impact on growth indices and biochemical responses in hybrid catfish","authors":"A. O. Kolawole, K. Adamu, I. Usman, Yakubu Manbe Mohammed, M. Aliyu-Paiko","doi":"10.21303/2504-5695.2023.002909","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002909","url":null,"abstract":"Globally the need to substitute fishmeal with other animal protein sources in the fish diet has gained more attention; which is occasioned by the increasing cost of fish. A 12-week trial was conducted to ascertain the growth indices and biochemical response of hybrid catfish (Clarias gariepinus(♀) and Heterobranchus bidorsalis (♂)) fed cockroach meal supplement. A total of 800 hybrid catfish were randomly distributed into four dietary treatments (Diet A- control feed), Diet B (50 % cockroach and 50 % fishmeal), Diet C (100 % cockroach meal) and Diet D (100 % fishmeal). Each treatment was replicated with 35 fish per experimental pond in a completely randomized design. Progressive weight gain was determined bi-weekly. At the end of the study period, fishes were randomly sacrificed for proximate compositions and plasma biochemical analyses (glucose, triglyceride, cholesterol, total protein, albumin) using standard protocols. The study revealed that growth parameters (Weight gain, Average weight gain, Protein efficiency ratio, Feed Conversion rate, Specific growth rate and Survival rate (SR) were influenced by the dietary treatments (p>0.05). The highest weight gain was recorded in Diet 1 (8847.73±292.65 g) > Diet 2 (7816.33±121.71 g)>Diet 3 (7811.67±566.08 g) >Diet 4 (7022.00±44.193 g). Similarly, the best feed conversion ratio was found in fish fed Diet A (10.12±0.01),>Diet B, C and D had the same values of 0.11 g respectively. There was no significant (p>0.05) difference in plasma biochemical indicators measured when compared to the control (Diet A). Thus, indicating that all the fish were healthy and had normal physiological activities. Cockroach meal can therefore, be used as a feed supplement without having any adverse effect on the growth and well-being of hybrid catfish fingerlings","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88290739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pinostrobin induces acute leukemia cell apoptosis via the regulation of miR-410-5p and SFRP5. Pinostrobin通过调控miR-410-5p和SFRP5诱导急性白血病细胞凋亡。

EUREKA: Life Sciences Pub Date : 2023-05-01 DOI: 10.2139/ssrn.4379242

Chosita Norkaew, S. Roytrakul, Sawanya Charoenlappanit, Siriwan Thaisakun, Dalina Tanyong

{"title":"Pinostrobin induces acute leukemia cell apoptosis via the regulation of miR-410-5p and SFRP5.","authors":"Chosita Norkaew, S. Roytrakul, Sawanya Charoenlappanit, Siriwan Thaisakun, Dalina Tanyong","doi":"10.2139/ssrn.4379242","DOIUrl":"https://doi.org/10.2139/ssrn.4379242","url":null,"abstract":"AIMS\u0000This study attempted to explore the mechanisms involved in pinostrobin (PN)-mediated acute leukemia cell apoptosis regulated by miR-410-5p.\u0000\u0000\u0000MATERIAL AND METHODS\u0000NB4 and MOLT-4 cells were cultured and treated with PN at the IC50 concentration. Apoptosis was examined by Annexin V-FITC/PI staining. RT-qPCR was used to measure the expression of caspase-3, BAK, BCL-W, and MCL-1. The target protein of PN was identified using LC-MS/MS followed by bioinformatic analysis. TargetScan, DIANA, and miRDB were used for the prediction of miRNAs involved in the PN-induced apoptosis mechanism. miRNA mimic transfection, RT-qPCR, and western blot analysis were performed to evaluate the regulatory effect of miRNA on its target and the involvement of miRNA in apoptosis induction by PN. In addition, the synergistic effect of PN and daunorubicin (DNR) were investigated by using the MTT assay.\u0000\u0000\u0000KEY FINDINGS\u0000The results showed that PN reduced cell viability and induced apoptosis in both leukemia cell lines. From the LC-MS/MS and bioinformatics analysis, SFRP5 and miR-410-5p were selected as a potential PN target protein and miRNA, respectively. After miRNA mimic transfection, miR-410-5p, which is an onco-miRNA, was decreased and led to increased apoptosis in both cell lines, indicating that this miRNA is involved in PN-mediated apoptosis mechanisms. Moreover, PN demonstrated a synergistic effect with DNR, suggesting that PN may be used in combination with conventional chemotherapy drugs.\u0000\u0000\u0000SIGNIFICANCE\u0000PN regulates the expression of miR-410-5p and SFRP5 to promote apoptosis in acute leukemia cells. It could be developed as an alternative treatment for leukemia in the future.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80682843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Medroxyprogesterone acetate-resistant endometrial cancer cells are susceptible to ferroptosis inducers. 抗醋酸甲孕酮的子宫内膜癌细胞对铁下垂诱导剂敏感。

EUREKA: Life Sciences Pub Date : 2023-05-01 DOI: 10.2139/ssrn.4355249

H. Murakami, M. Hayashi, S. Terada, M. Ohmichi

{"title":"Medroxyprogesterone acetate-resistant endometrial cancer cells are susceptible to ferroptosis inducers.","authors":"H. Murakami, M. Hayashi, S. Terada, M. Ohmichi","doi":"10.2139/ssrn.4355249","DOIUrl":"https://doi.org/10.2139/ssrn.4355249","url":null,"abstract":"AIMS\u0000Medroxyprogesterone acetate (MPA) is the most common fertility-sparing treatment in patients with early-stage endometrial cancer. If MPA treatment fails, hysterectomy is recommended. Thus, there is an urgent need for novel treatment approaches for MPA-resistant endometrial cancer patients who wish to preserve their fertility. Ferroptosis is a recently discovered type of regulated cell death caused by the excessive accumulation of reactive oxygen species (ROS), followed by aberrant lipid peroxidation. Recent studies have shown that inducing ferroptosis is a potential therapeutic strategy for cancer. However, the role of ferroptosis in endometrial cancer treatment remains to be discussed. We therefore investigated the effects of ferroptosis inducers on MPA-resistant endometrial cancer cells.\u0000\u0000\u0000MAIN METHODS\u0000The levels of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), the main mediators of ferroptosis, were examined. Cell viability was evaluated after treatment with the ferroptosis inducers sulfasalazine, erastin, or RSL3. The degree of intracellular oxidative stress after treatment with these drugs was evaluated by the glutathione level, ROS level, ferrous iron level, lipid peroxidation and changes in mitochondrial morphology. The effect of ferroptosis inducers in vivo was also examined.\u0000\u0000\u0000KEY FINDINGS\u0000The expression of SLC7A11 and GPX4 in MPA-resistant ECC-1 cells decreased in comparison to parental ECC-1 cells. Sulfasalazine, erastin, and RSL3 significantly reduced cell viability and increased intracellular oxidative stress in MPA-resistant ECC-1 cells. Ferroptosis inducers also suppressed in vivo tumor growth more effectively in MPA-resistant ECC-1.\u0000\u0000\u0000SIGNIFICANCE\u0000Treatment with ferroptosis inducers could be a novel therapeutic approach for MPA-resistant endometrial cancer.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81301839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Topiramate treatment during the peripubertal period does not alter aortic endothelial function in female Wistar rats. 托吡酯治疗在青春期周围没有改变雌性Wistar大鼠的主动脉内皮功能。

EUREKA: Life Sciences Pub Date : 2023-05-01 DOI: 10.2139/ssrn.4333542

D. G. da Silva, K. F. Moura, A. C. de Souza, Kenny Gutemberg Nunes Silva, C. B. Vidigal, Juliana da Silva Jezuíno, Rafaella Cardoso Gravena, G. G. Pelosi, D. C. Gerardin, Maria do Carmo Franco, G. Ceravolo

{"title":"Topiramate treatment during the peripubertal period does not alter aortic endothelial function in female Wistar rats.","authors":"D. G. da Silva, K. F. Moura, A. C. de Souza, Kenny Gutemberg Nunes Silva, C. B. Vidigal, Juliana da Silva Jezuíno, Rafaella Cardoso Gravena, G. G. Pelosi, D. C. Gerardin, Maria do Carmo Franco, G. Ceravolo","doi":"10.2139/ssrn.4333542","DOIUrl":"https://doi.org/10.2139/ssrn.4333542","url":null,"abstract":"AIMS\u0000This study aimed to evaluate the short- and long-term adverse effects of blood pressure (BP), vascular endothelial function, and estrogen receptor (ERα and ERβ) modulation on endothelial function in female Wistar rats treated with topiramate (TPM), an antiepileptic drug, during the peripubertal period.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Female Wistar rats were treated with TPM (41 mg/kg) or water (CTR group) by gavage from postnatal day (PND) 28 to 50 (peripubertal phase). At the end of the treatment, the TPM and CTR rats were divided into two groups and evaluated after 24 h or from PND 85 (adulthood). The rats were evaluated for: thoracic aorta reactivity to phenylephrine (Phenyl), acetylcholine (ACh), and sodium nitroprusside (SNP); aortic ring reactivity after ERα and ERβ antagonism; and BP.\u0000\u0000\u0000KEY FINDINGS\u0000It was observed that vascular response to Phenyl, ACh, and SNP was similar between TPM and CTR rats in the short- and long-term evaluations. In addition, the ER antagonism did not interfere with aortic contraction or relaxation in either TPM or CTR.\u0000\u0000\u0000SIGNIFICANCE\u0000Taken together, the results show that TPM treatment during the peripubertal period does not alter aortic endothelial function and its estrogen modulation via classic ER in female Wistar rats, suggesting that TPM treatment in this period is safe for the vascular system.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76091530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Student Researcher Spotlight 学生研究重点

EUREKA: Life Sciences Pub Date : 2023-04-30 DOI: 10.29173/eureka28788

Juliette Eshleman

引用次数: 0

From Dr. Lisa Willis 丽莎·威利斯博士

EUREKA: Life Sciences Pub Date : 2023-04-30 DOI: 10.29173/eureka28786

L. Willis

引用次数: 0

Student Researcher Spotlight 学生研究重点

EUREKA: Life Sciences Pub Date : 2023-04-30 DOI: 10.29173/eureka28789

Matthew Birtle

引用次数: 0