EUREKA: Life Sciences最新文献_第10页

The Light We Cannot See 我们看不见的光

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60446-0

引用次数: 0

Wave Springs Met Market Needs 波浪泉满足市场需求

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60452-6

引用次数: 0

Skip Drive Offers More Control 跳过驱动器提供更多的控制

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60447-2

引用次数: 0

Rugged Connectors Withstand Harsh Environments 坚固耐用的连接器可抵御恶劣环境

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60453-8

引用次数: 0

Power Cut 停电

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60437-x

引用次数: 0

Direct-Drive Cuts Cost of Ownership 直接驱动降低了拥有成本

EUREKA: Life Sciences Pub Date : 2023-02-01 DOI: 10.12968/s0261-2097(23)60445-9

引用次数: 0

Prostacyclin receptor agonists induce DUSP1 to inhibit pulmonary artery smooth muscle cell proliferation. 前列环素受体激动剂诱导DUSP1抑制肺动脉平滑肌细胞增殖。

EUREKA: Life Sciences Pub Date : 2023-01-03 DOI: 10.2139/ssrn.4249928

H. Maruyama, S. Sakai, L. Dewachter, C. Dewachter, B. Rondelet, R. Naeije, M. Ieda

{"title":"Prostacyclin receptor agonists induce DUSP1 to inhibit pulmonary artery smooth muscle cell proliferation.","authors":"H. Maruyama, S. Sakai, L. Dewachter, C. Dewachter, B. Rondelet, R. Naeije, M. Ieda","doi":"10.2139/ssrn.4249928","DOIUrl":"https://doi.org/10.2139/ssrn.4249928","url":null,"abstract":"AIMS\u0000Upregulated p38MAPK signaling is implicated in the accelerated proliferation of pulmonary artery smooth muscle cells (PA-SMCs) and the pathogenesis of pulmonary artery remodeling observed in pulmonary arterial hypertension (PAH). Previously, we reported that after endothelin-1 (ET-1) pretreatment, bone morphogenetic protein 2 (BMP2) activates p38MAPK signaling and accelerates PA-SMC proliferation. The activity of p38MAPK signaling is tightly regulated by the inactivation of dual-specificity phosphatase 1 (DUSP1). Activated p38MAPK induces DUSP1 expression, forming a negative feedback loop. Prostacyclin IP receptor agonists (prostacyclin and selexipag) are used to treat PAH. In this study, we aimed to verify whether IP receptor agonists affect DUSP1 expression and accelerate the proliferation of PA-SMCs.\u0000\u0000\u0000MAIN METHODS\u0000PA-SMCs were treated with BMP2, ET-1, prostacyclin, and MRE-269, an active metabolite of selexipag, either alone or in combination. We quantified mRNA expressions using real-time quantitative polymerase chain reaction. Pulmonary artery specimens and PA-SMCs were obtained during lung transplantation in patients with PAH.\u0000\u0000\u0000KEY FINDINGS\u0000Both prostacyclin and MRE-269 increased DUSP1 expression. Combined treatment with BMP2 and ET-1 induced cyclin D1 and DUSP1 expression and increased PA-SMC proliferation. MRE-269 attenuated BMP2/ET-1-induced cell proliferation. ET-1 increased DUSP1 expression in PA-SMCs from control patients but not in PA-SMCs from patients with PAH.\u0000\u0000\u0000SIGNIFICANCE\u0000This study showed that the p38MAPK/DUSP1 negative feedback loop is impaired in PAH, contributing to unregulated p38MAPK activation and PA-SMC hyperplasia. IP receptor agonist MRE-269 increases DUSP1 expression and inhibit p38MAPK-mediated PA-SMC proliferation. Future elucidation of the detailed mechanism underlying reduced DUSP1 expression would be informative for PAH treatment.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89701088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion. ras转染的人乳腺肿瘤细胞通过与细胞粘附相关的机制对光动力治疗产生抗性。

EUREKA: Life Sciences Pub Date : 2022-12-01 DOI: 10.2139/ssrn.4253503

L. Rodriguez, G. D. Di Venosa, Martín A. Rivas, Á. Juarranz, F. Sanz‐Rodríguez, A. Casas

{"title":"Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion.","authors":"L. Rodriguez, G. D. Di Venosa, Martín A. Rivas, Á. Juarranz, F. Sanz‐Rodríguez, A. Casas","doi":"10.2139/ssrn.4253503","DOIUrl":"https://doi.org/10.2139/ssrn.4253503","url":null,"abstract":"AIMS\u0000Photodynamic therapy (PDT) is a treatment modality for several cancers involving the administration of a tumour-localising photosensitiser (PS) and its subsequent activation by light, resulting in tumour damage. Ras oncogenes have been strongly associated with chemo- and radio-resistance. Based on the described roles of adhesion and cell morphology on drug resistance, we studied if the differences in shape, cell-extracellular matrix and cell-cell adhesion induced by Ras transfection, play a role in the resistance to PDT.\u0000\u0000\u0000MATERIALS AND METHODS\u0000We employed the human normal breast HB4a cells transfected with H-RAS and a panel of five PSs.\u0000\u0000\u0000KEY FINDINGS\u0000We found that resistance to PDT of the HB4a-Ras cells employing all the PSs, increased between 1.3 and 2.5-fold as compared to the parental cells. There was no correlation between resistance and intracellular PS levels or PS intracellular localisation. Even when Ras-transfected cells present lower adherence to the ECM proteins, this does not make them more sensitive to PDT or chemotherapy. On the contrary, a marked gain of resistance to PDT was observed in floating cells as compared to adhesive cells, accounting for the higher ability conferred by Ras to survive in conditions of decreased cell-extracellular matrix interactions. HB4a-Ras cells displayed disorganisation of actin fibres, mislocalised E-cadherin and vinculin and lower expression of E-cadherin and β1-integrin as compared to HB4a cells.\u0000\u0000\u0000SIGNIFICANCE\u0000Knowledge of the mechanisms of resistance to photodamage in Ras-overexpressing cells may lead to the optimization of the combination of PDT with other treatments.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84293912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of NAC assisted insulin on cholesterol metabolism disorders in canine type 1 diabetes mellitus. NAC辅助胰岛素对犬1型糖尿病胆固醇代谢紊乱的影响。

EUREKA: Life Sciences Pub Date : 2022-12-01 DOI: 10.2139/ssrn.4249942

Shuzhou Wang, Haihua Huo, Haitong Wu, F. Ma, Jianzhao Liao, Xinrun Li, Qingyu Ding, Zhaoxin Tang, Jianying Guo

{"title":"Effects of NAC assisted insulin on cholesterol metabolism disorders in canine type 1 diabetes mellitus.","authors":"Shuzhou Wang, Haihua Huo, Haitong Wu, F. Ma, Jianzhao Liao, Xinrun Li, Qingyu Ding, Zhaoxin Tang, Jianying Guo","doi":"10.2139/ssrn.4249942","DOIUrl":"https://doi.org/10.2139/ssrn.4249942","url":null,"abstract":"Type 1 diabetes mellitus (T1DM) is a metabolic disease characterized by insulin deficiency and often accompanied by hypercholesterolemia. NAC is an effective antioxidative drug, but its application in the treatment of diabetes is still rare. A total of forty beagles were randomly divided into five groups: control group, DM group, INS group, INS with NAC group, and NAC group. The experiment lasted for 120 days. Results revealed that biochemical criterion increased in the DM group, while the indicators significantly decreased on the INS combined with NAC treatment group. Moreover, the insulin released test demonstrated that the model of T1DM was successfully constructed. The result of B ultrasound of gallbladder showed remarkable cholestasis in DM group. The cholesterol metabolism-related enzyme activity (HMGCR and SQLE) was evidently increased in DM group, but decreased in INS and NAC group. The content of TG, LDL-c, and HDL-c in liver was detected by the kit, and it was found that the content of TG, LDL-c, and HDL-c in DM group were reduced. Histopathological observation revealed that the cholestasis of liver cells and hepatic cords were disordered in DM group, the symptoms were alleviated under INS and NAC treatment. Additionally, the protein and mRNA expression of HMGCR and LDLR were obviously increased in DM group, but down regulated in INS and NAC treatment group. Overall, the liver function injury and secondary hypercholesterolemia can be found in T1DM canines, and NAC can relieve cholesterol metabolism disorder in the treatment of canine T1DM.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86470014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antibacterial activity and metabolomic analysis of linalool against bovine mastitis pathogen Streptococcus agalactiae. 芳樟醇对牛乳腺炎病原菌无乳链球菌的抑菌活性及代谢组学分析。

EUREKA: Life Sciences Pub Date : 2022-12-01 DOI: 10.2139/ssrn.4257878

T. Liang, G. Huo, Lele Chen, Ling Ding, Jian Wu, Ji Zhang, Rongmin Wang

{"title":"Antibacterial activity and metabolomic analysis of linalool against bovine mastitis pathogen Streptococcus agalactiae.","authors":"T. Liang, G. Huo, Lele Chen, Ling Ding, Jian Wu, Ji Zhang, Rongmin Wang","doi":"10.2139/ssrn.4257878","DOIUrl":"https://doi.org/10.2139/ssrn.4257878","url":null,"abstract":"Streptococcus agalactiae is among the major causative pathogens of bovine mastitis, as well as crucial pathogen leading to human morbidity and mortality. Being a promising natural antibacterial agent, linalool has been broadly applied in medicine and food processing. However, its antibacterial effect against S. agalactiae has barely been elucidated. This study is the first to investigate the antibacterial activity and action mechanism of linalool against S. agalactiae causing bovine mastitis. Linalool exhibited significant antibacterial activity against S. agalactiae, with an inhibition zone diameter of 23 mm and a minimum inhibitory concentration of 1.875 μL/mL. In addition, linalool damaged cell structural integrity of S. agalactiae, leading to the leakage of intracellular components (alkaline phosphatase, nucleic acids and protein). Linalool also exhibited a scavenging effect on biofilm. Moreover, untargeted metabolomics analysis revealed that linalool stress substantially disrupted intracellular metabolism of S. agalactiae. Linalool caused energy metabolism disorder, and obstructed nucleic acid synthesis in S. agalactiae. Furthermore, downregulation of amino acids (e.g., proline, alanine) and upregulation of saturated fatty acids provide strong evidence for linalool induced cell wall and membrane damage. Overall, linalool exhibited strong antibacterial activity against S. agalactiae by destroying the cell structure and disrupting intracellular metabolism. This study provides a new insight and theoretical foundation for linalool application in preventing S. agalactiae infection.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81405798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1