V Hopwood, D W Warnock, J D Milne, T Crowley, C T Horrocks, P K Taylor
{"title":"Evaluation of a new slide latex agglutination test for diagnosis of vaginal candidosis.","authors":"V Hopwood, D W Warnock, J D Milne, T Crowley, C T Horrocks, P K Taylor","doi":"10.1007/BF02013092","DOIUrl":"https://doi.org/10.1007/BF02013092","url":null,"abstract":"<p><p>A new commercial slide latex particle agglutination test for rapid (2 min) diagnosis of vaginal candidosis was evaluated and compared with conventional methods. Of the 263 women studied, 63 (23.9%) had yeasts in the vagina. Clinical signs of vulvitis or vaginitis were seen in 23 women (8.8%) and 40 (15.2%) were harbouring yeasts without clinical signs. Yeast counts were generally higher in women with clinical signs of vaginal candidosis than in those without. The test was positive in 15 of the 23 women (65.2%) with clinical signs, the incidence of a positive test increasing in direct proportion to the amount of yeasts isolated. The test's sensitivity, specificity and predictive values were comparable to those of microscopy and culture. Being both rapid and simple to perform, this new test offers a useful alternative to conventional methods for the diagnosis of vaginal candidosis.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"392-4"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14785999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Couetdic, P Plesiat, R Chambers, J Y Cahn, Y Michel-Briand
{"title":"Three cases of bacteremia with group Ve-2 bacteria in France.","authors":"G Couetdic, P Plesiat, R Chambers, J Y Cahn, Y Michel-Briand","doi":"10.1007/BF02013125","DOIUrl":"https://doi.org/10.1007/BF02013125","url":null,"abstract":"","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"499-500"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14786697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of disk approximation and agar dilution induction tests for demonstration of in vitro antagonism of cefotaxime by cefoxitin in Enterobacter species.","authors":"S W Chandler, F S Nolte","doi":"10.1007/BF02013114","DOIUrl":"https://doi.org/10.1007/BF02013114","url":null,"abstract":"<p><p>Cefoxitin-resistant clinical isolates of Enterobacter spp. demonstrated inducible beta-lactamase activity in 31 of 47 (65.9%) disk approximation tests and in 32 of 52 (63.5%) agar dilution induction tests. Agreement between the two results was only 72.3%, and 80.9% of the strains were positive in at least one induction test. Lack of valid interpretive criteria preclude their routine use in the clinical laboratory.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"476-8"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14622633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D L Dworzack, M P Pugsley, C C Sanders, E A Horowitz
{"title":"Emergence of resistance in gram-negative bacteria during therapy with expanded-spectrum cephalosporins.","authors":"D L Dworzack, M P Pugsley, C C Sanders, E A Horowitz","doi":"10.1007/BF02013110","DOIUrl":"https://doi.org/10.1007/BF02013110","url":null,"abstract":"<p><p>To assess the clinical importance of emergence of beta-lactam resistance caused by stable derepression of chromosomal beta-lactamases, sequential cultures from patients treated with expanded-spectrum cephalosporins were monitored for the persistence of bacteria possessing these enzymes. Antibiotic susceptibilities and beta-lactamase production before and after cefoxitin induction were determined in sequential isolates of individual bacterial strains. Of 49 strains isolated from 44 patients, 25 strains (51%) were eradicated by cephalosporin therapy, 17 strains (35%) persisted with unchanged susceptibility in sequential cultures, and 7 strains (14%) from 7 patients developed multiple beta-lactam resistance during cephalosporin therapy. In 6 of the 7 strains, resistance was associated with stable derepression of beta-lactamases. In the patient group whose strains developed resistance, subsequent use of non-beta-lactam antibiotics was more frequent and mortality was higher.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"456-9"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14622632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation of Corynebacterium group D2 from clinical specimens.","authors":"B Van Bosterhaut, G Claeys, J Gigi, G Wauters","doi":"10.1007/BF02013099","DOIUrl":"https://doi.org/10.1007/BF02013099","url":null,"abstract":"<p><p>A total of 38 strains of Corynebacterium Group D2 isolated from clinical specimens in Belgian laboratories were characterized by pronounced urease activity, inability to acidify sugars and to reduce nitrates and multiresistance to antibiotics. Two strains were involved in clinical diseases. The organisms were found to have a more opportunistic than pathogenic behaviour.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"418-9"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14786689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J N Katchaki, F B Bronkhorst, G P van Berge Henegouwen
{"title":"Lack of invasiveness of Campylobacter pylori.","authors":"J N Katchaki, F B Bronkhorst, G P van Berge Henegouwen","doi":"10.1007/BF02013126","DOIUrl":"https://doi.org/10.1007/BF02013126","url":null,"abstract":"","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"500"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14786698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incidence of inducible beta-lactamases in gram-negative septicemia isolates from twenty-nine European laboratories. European Study Group on Antibiotic Resistance.","authors":"","doi":"10.1007/BF02013111","DOIUrl":"https://doi.org/10.1007/BF02013111","url":null,"abstract":"<p><p>In 1984 the European Study Group on Antibiotic Resistance (ESGAR), which is made up of 29 laboratories in 12 European countries, consecutively collected gram-negative bacilli and staphylococci isolates from blood and using the microdilution method performed susceptibility testing with 11 beta-lactam antibiotics. A total of 2,578 isolates were collected; 68% were gram-negatives and 32% staphylococci. Pseudomonas spp. accounted for 12% of the strains, Enterobacter spp. 7%, Serratia spp. 3%, indole-positive Proteus spp. 1%, Citrobacter spp. and Morganella spp. 0.9% each. Strains with inducible beta-lactamases were detected by the cefoxitin disc diffusion method in 11% of all gram-negatives and in 67% of the relevant species. The production of inducible beta-lactamase was confirmed by elevated MICs to and decreased killing by piperacillin, cefotaxime and ceftazidime after induction of enzyme production with low concentrations of cefoxitin. This phenomenon was not observed with mecillinam or the new penem Sch 34343.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"460-6"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14248855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A L Barry, G H Miller, R S Hare, C Thornsberry, R N Jones
{"title":"Netilmicin disk susceptibility tests: effect of cations on the MIC correlates.","authors":"A L Barry, G H Miller, R S Hare, C Thornsberry, R N Jones","doi":"10.1007/BF02013098","DOIUrl":"https://doi.org/10.1007/BF02013098","url":null,"abstract":"<p><p>When testing Pseudomonas aeruginosa against netilmicin, MICs were markedly affected by the concentration of cations added to the test medium. A susceptible disk test result (zone greater than or equal to 15 mm) corresponded to MIC less than or equal to 4.0 micrograms/ml in unsupplemented broth, less than or equal to 12 micrograms/ml in broth with half the usual amount of cations and less than or equal to 32 micrograms/ml in broth with the recommended concentration of cations. Tests with 30 micrograms netilmicin disks best predicted susceptibility as determined by MICs in broth without added cations. When the MICs were determined in cation supplemented broth, the number of interpretive discrepancies increased to an unacceptably high level.</p>","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 4","pages":"416-7"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02013098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14248853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}