European cells & materials最新文献_第8页

Dental pulp inflammatory/immune response to a chitosan-enriched fibrin hydrogel in the pulpotomised rat incisor. 大鼠切牙后牙髓对富含壳聚糖的纤维蛋白水凝胶的炎症/免疫反应。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-08-20 DOI: 10.22203/eCM.v040a05

E Renard, J Amiaud, L Delbos, C Charrier, A Montembault, M Ducret, J-C Farges, L David, B Alliot-Licht, A Gaudin

{"title":"Dental pulp inflammatory/immune response to a chitosan-enriched fibrin hydrogel in the pulpotomised rat incisor.","authors":"E Renard, J Amiaud, L Delbos, C Charrier, A Montembault, M Ducret, J-C Farges, L David, B Alliot-Licht, A Gaudin","doi":"10.22203/eCM.v040a05","DOIUrl":"https://doi.org/10.22203/eCM.v040a05","url":null,"abstract":"Current pulpotomy is limited in its ability to induce regeneration of the dental-pulp (DP) complex. Hydrogels are reported to be well-suited for tissue engineering and are unlikely to induce an inflammatory response that might damage the remaining tissue. The present study investigated the molecular and cellular actors in the early inflammatory/immune response and deciphered M1/M2 macrophage polarisation to a chitosan-enriched fibrin hydrogel in pulpotomised rat incisors. Both fibrin and fibrin-chitosan hydrogels induced a strong increase in interleukin-6 (IL-6) transcript in the DP when compared to the DP of untreated teeth. Gene expression of other inflammatory mediators was not significantly modified after 3 h. In the viable DP cell population, the percentage of leukocytes assessed by flow cytometry was similar to fibrin and fibrin-chitosan hydrogels after 1 d. In this leukocyte population, the proportion of granulocytes increased beneath both hydrogels whereas the antigen-presenting cell, myeloid dendritic cells, T cells and B cells decreased. The natural killer (NK) cell population was significantly decreased only in DPs from teeth treated with fibrin-chitosan hydrogel. Immunolabeling analysis of the DP/hydrogel interface showed accumulation of neutrophil granulocytes in contact with both hydrogels 1 d after treatment. The DP close to this granulocyte area contained M2 but no M1 macrophages. These data collectively demonstrated that fibrin-chitosan hydrogels induced an inflammatory/immune response similar to that of the fibrin hydrogel. The results confirmed the potential clinical use of fibrin-chitosan hydrogel as a new scaffold for vital-pulp therapies.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"40 ","pages":"74-87"},"PeriodicalIF":3.1,"publicationDate":"2020-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38284176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Enrichment and preserved functionality of multipotential stromal cells in bone marrow concentrate processed by vertical centrifugation. 垂直离心处理骨髓浓缩液中多电位间质细胞的富集及功能保存。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-08-04 DOI: 10.22203/eCM.v040a04

J J El-Jawhari, D C Ilas, W Jones, R Cuthbert, E Jones, P V Giannoudis

{"title":"Enrichment and preserved functionality of multipotential stromal cells in bone marrow concentrate processed by vertical centrifugation.","authors":"J J El-Jawhari, D C Ilas, W Jones, R Cuthbert, E Jones, P V Giannoudis","doi":"10.22203/eCM.v040a04","DOIUrl":"https://doi.org/10.22203/eCM.v040a04","url":null,"abstract":"The concentration of bone marrow (BM) aspirate (BMA) is increasingly valued for bone and cartilage repair, particularly with the rarity and donor-variability of BM-multipotential stromal cells (BM-MSCs). The present study aimed to assess BM-MSC yield following BM concentration using a fast and compact-sized vertical centrifugation system. BMA concentrate (BMAC) was separated in a 1 min process and collected easily after an automatic discarding of plasma and red blood cells. A significant increase in CD45low CD271high cells per BMAC volume (measured using flow-cytometry) was noted (4-fold, p = 0.0001). Additionally, the vertical centrifugation system helped to enrich colony numbers (assessed by CFU-F assays) in BMAC comparably with conventional centrifugation systems, BioCUE™ and SmartPReP-2® (4.3-fold, 4.6-fold and 3-fold, respectively). Next, a functional assessment of BM-MSCs processed by vertical centrifugation was performed, and MSC viability and proliferation were not affected. Also, these BM-MSCs showed similar alkaline phosphatase and calcium levels to those of BMA-MSCs when osteogenically induced. Furthermore, glycosaminoglycans and Nile red levels in addition to the gene expression assays confirmed that there was no significant change in chondrogenic or adipogenic abilities between BMA-MSCs and BMAC-MSCs. The expression levels of selected angiogenic and immunomodulatory mediators were also similar between the two groups. Collectively, the vertical centrifugation system helped to enrich BM-MSCs effectively, while maintaining cell viability and functions. Thus, such a vertical centrifugation system for BM concentration can be valuable for various regenerative therapies.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"40 ","pages":"58-73"},"PeriodicalIF":3.1,"publicationDate":"2020-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38227080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dual local drug delivery of vancomycin and farnesol for mitigation of MRSA infection in vivo - a pilot study. 万古霉素和法尼醇双重局部给药减轻体内MRSA感染的初步研究。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-07-16 DOI: 10.22203/eCM.v040a03

U C Woelfle, T Briggs, S Bhattacharyya, H Qu, N Sheth, C Knabe, P Ducheyne

{"title":"Dual local drug delivery of vancomycin and farnesol for mitigation of MRSA infection in vivo - a pilot study.","authors":"U C Woelfle, T Briggs, S Bhattacharyya, H Qu, N Sheth, C Knabe, P Ducheyne","doi":"10.22203/eCM.v040a03","DOIUrl":"https://doi.org/10.22203/eCM.v040a03","url":null,"abstract":"Surgical site infections after orthopaedic surgery using fracture fixation devices or endosseous implants create major surgical challenges with severe adverse effects, such as osteomyelitis. These infections are frequently caused by Staphylococcus aureus, often with high resistance to antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA). Due to the formation of impenetrable biofilms on implant surfaces, systemic antibiotic treatment has become exceedingly difficult. New solutions are pursued by combining several drugs using a controlled delivery system from specifically engineered implant surfaces. A sol-gel coating on titanium implants was previously developed with 20 wt % vancomycin and 30 wt % farnesol, with suppression of MRSA in vitro. The present study investigated the efficacy of sol-gel film coatings for controlled dual local delivery over 4 weeks utilising a rat infection model. The findings confirmed the viability of this new concept in vivo based on the differences observed between coatings containing vancomycin alone (SGV) and the dual-drug-containing coating with vancomycin and farnesol (SGVF). While both the SGVF and SGV coatings facilitated excellent preservation of the osseous microarchitecture, SGVF coating displayed a slightly higher potency for suppressing MRSA infiltration than SGV, in combination with a lower reactive bone remodelling activity, most likely by disturbing biofilm formation. The next step for advancing the concept of dual-drug delivery from sol-gel coatings to the clinic and confirming the promising effect of the SGVF coatings on reactive bone remodelling and suppressing MRSA infiltration is a study in a larger animal species with longer time points.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"40 ","pages":"38-57"},"PeriodicalIF":3.1,"publicationDate":"2020-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38187695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture system. 用山羊离体加载的椎间盘培养系统模拟中度退变的椎间盘分解代谢环境。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-07-16 DOI: 10.22203/eCM.v040a02

C Me Rustenburg, J W Snuggs, K S Emanuel, A Thorpe, C Sammon, C L Le Maitre, T H Smit

{"title":"Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture system.","authors":"C Me Rustenburg, J W Snuggs, K S Emanuel, A Thorpe, C Sammon, C L Le Maitre, T H Smit","doi":"10.22203/eCM.v040a02","DOIUrl":"https://doi.org/10.22203/eCM.v040a02","url":null,"abstract":"Low-back pain affects 80 % of the world population at some point in their lives and 40 % of the cases are attributed to intervertebral disc (IVD) degeneration. Over the years, many animal models have been developed for the evaluation of prevention and treatment strategies for IVD degeneration. Ex vivo organ culture systems have also been developed to better control mechanical loading and biochemical conditions, but a reproducible ex vivo model that mimics moderate human disc degeneration is lacking. The present study described an ex vivo caprine IVD degeneration model that simulated the changes seen in the nucleus pulposus during moderate human disc degeneration. Following pre-load under diurnal, simulated physiological loading (SPL) conditions, lumbar caprine IVDs were degenerated enzymatically by injecting collagenase and chondroitinase ABC (cABC). After digestion, IVDs were subjected to SPL for 7 d. No intervention and phosphate-buffered saline injection were used as controls. Disc deformation was continuously monitored to assess disc height recovery. Histology and immunohistochemistry were performed to determine the histological grade of degeneration, matrix expression, degrading enzyme and catabolic cytokine expression. Injection of collagenase and cABC irreversibly affected the disc mechanical properties. A decrease in extracellular matrix components was found, along with a consistent increase in degradative enzymes and catabolic proteins [interleukin (IL)-1β, -8 and vascular endothelial growth factor (VEGF)]. The changes observed were commensurate with those seen in moderate human-IVD degeneration. This model should allow for controlled ex vivo testing of potential biological, cellular and biomaterial treatments of moderate human-IVD degeneration.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"40 ","pages":"21-37"},"PeriodicalIF":3.1,"publicationDate":"2020-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22203/eCM.v040a02","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38166108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The current state of PEEK implant osseointegration and future perspectives: a systematic review. PEEK种植体骨整合的现状及未来展望:系统综述。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-07-15 DOI: 10.22203/eCM.v040a01

N AlOtaibi, K Naudi, D Conway, A Ayoub

{"title":"The current state of PEEK implant osseointegration and future perspectives: a systematic review.","authors":"N AlOtaibi, K Naudi, D Conway, A Ayoub","doi":"10.22203/eCM.v040a01","DOIUrl":"https://doi.org/10.22203/eCM.v040a01","url":null,"abstract":"Polyetheretherketone (PEEK) has been considered as an alternative to replace surgical metal implants. Several medical applications, including dental and orthopaedic implants, need confirmed osseointegration before functional loading. The present study aims at providing a comprehensive systematic review of the evidence on PEEK implants' osseointegration. A systematic search was conducted using Cochrane library, MEDLINE (PubMed), Ovid MEDLINE, Web of Science and EMBASE databases. Publications were identified in accordance with specific inclusion and exclusion criteria. Eligibility screening, data extraction and quality assessment were performed. The review protocol was registered in PROSPERO (CRD42018116061). A total of 55 articles were reviewed and 29 of the most relevant that met the inclusion criteria were selected. Heterogeneity was identified among the included studies. Several approaches have been applied to enhance PEEK osseointegration, with most in vivo studies conducted on small-scale animal models but no study evaluating the osseointegration of PEEK under cyclic loading. However, PEEK modifications are demonstrated to enhance osseointegration preclinically. Collectively, the present review shows a shortage of evidence, including a lack of comprehensive assessment of osseointegration, the need for large-animal-model tests, the need to assess the effect of loading on the implants and the lack of randomised controlled clinical trials.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"40 ","pages":"1-20"},"PeriodicalIF":3.1,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38155267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Link N suppresses interleukin-1β-induced biological effects on human osteoarthritic cartilage. Link N抑制白介素-1β诱导的人骨关节炎软骨生物学效应。

IF 3.1 3区医学

European cells & materials Pub Date : 2020-01-15 DOI: 10.22203/eCM.v039a04

M Alaqeel, M P Grant, L M Epure, O Salem, A AlShaer, O L Huk, S G Bergeron, D J Zukor, R Kc, H-J Im, A N Anbazhagan, J Antoniou, F Mwale

{"title":"Link N suppresses interleukin-1β-induced biological effects on human osteoarthritic cartilage.","authors":"M Alaqeel, M P Grant, L M Epure, O Salem, A AlShaer, O L Huk, S G Bergeron, D J Zukor, R Kc, H-J Im, A N Anbazhagan, J Antoniou, F Mwale","doi":"10.22203/eCM.v039a04","DOIUrl":"https://doi.org/10.22203/eCM.v039a04","url":null,"abstract":"Osteoarthritis (OA) is a disease of diarthrodial joints associated with extracellular matrix proteolytic degradation under inflammatory conditions, pain and disability. Currently, there is no therapy to prevent, reverse or modulate the disease course. The present study aimed at evaluating the regenerative potential of Link N (LN) in human OA cartilage in an inflammatory milieu and determining if LN could affect pain-related behaviour in a knee OA mouse injury model. Osteo-chondro OA explants and OA chondrocytes were treated with LN in the presence of interleukin-1β (IL-1β) to simulate an osteoarthritic environment. Quantitative von Frey polymerase chain reaction and Western blotting were performed to determine the effect of LN on matrix protein synthesis, catabolic enzymes, cytokines and nerve growth factor expression. Partial medial meniscectomy (PMM) was performed on the knee of C57BL/6 mice and, 12 weeks post-surgery, mice were given a 5 µg intra-articular injection of LN or phosphate-buffered saline. A von Frey test was conducted over 24 h to measure the mechanical allodynia in the hind paw. LN modulated proteoglycan and collagen synthesis in human OA cartilage through inhibition of IL-1β-induced biological effects. LN also supressed IL-1β-induced upregulation of cartilage-degrading enzymes and inflammatory molecules in OA chondrocytes. Upon investigation of the canonical signalling pathways IL-1β and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), LN resulted to significantly inhibit NF-κB activation in a dose-dependent manner. In addition, LN suppressed mechanical allodynia in an OA PMM mouse model. Results supported the concept that LN administration could provide therapeutic potential in OA.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"39 ","pages":"65-76"},"PeriodicalIF":3.1,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10184629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

DEXAMETHASONE: CHONDROPROTECTIVE CORTICOSTEROID OR CATABOLIC KILLER? 地塞米松:保护软骨的皮质类固醇还是分解代谢杀手?

IF 3.1 3区医学

European cells & materials Pub Date : 2019-11-22 DOI: 10.22203/eCM.v038a17

R. Black, A. Grodzinsky

{"title":"DEXAMETHASONE: CHONDROPROTECTIVE CORTICOSTEROID OR CATABOLIC KILLER?","authors":"R. Black, A. Grodzinsky","doi":"10.22203/eCM.v038a17","DOIUrl":"https://doi.org/10.22203/eCM.v038a17","url":null,"abstract":"While glucocorticoids have been used for over 50 years to treat rheumatoid and osteoarthritis pain, the prescription of glucocorticoids remains controversial because of potentially harmful side effects at the molecular, cellular and tissue levels. One member of the glucocorticoid family, dexamethasone (DEX) has recently been demonstrated to rescue cartilage matrix loss and chondrocyte viability in animal studies and cartilage explant models of tissue injury and post-traumatic osteoarthritis, suggesting the possibility of DEX as a disease-modifying drug if used appropriately. However, the literature on the effects of DEX on cartilage reveals conflicting results on the drug’s safety, depending on the dose and duration of DEX exposure as well as the model system used. Overall, DEX has been shown to protect against arthritis-related changes in cartilage structure and function, including matrix loss, inflammation and cartilage viability. These beneficial effects are not always observed in model systems using initially healthy cartilage or isolated chondrocytes, where many studies have reported significant increases in chondrocyte apoptosis. It is crucially important to understand under what conditions DEX may be beneficial or harmful to cartilage and other joint tissues and to determine potential for safe use of this glucocorticoid in the clinic as a disease-modifying drug.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"38 1","pages":"246 - 263"},"PeriodicalIF":3.1,"publicationDate":"2019-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22203/eCM.v038a17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49145706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Vibration treatment modulates macrophage polarisation and enhances early inflammatory response in oestrogen-deficient osteoporotic-fracture healing. 振动治疗可调节巨噬细胞极化并增强雌激素缺乏的骨质疏松骨折愈合中的早期炎症反应。

IF 3.1 3区医学

European cells & materials Pub Date : 2019-11-07 DOI: 10.22203/eCM.v038a16

S. Chow, Y. Chim, J. Wang, N. Zhang, R. Wong, N. Tang, K. Leung, W. Cheung

{"title":"Vibration treatment modulates macrophage polarisation and enhances early inflammatory response in oestrogen-deficient osteoporotic-fracture healing.","authors":"S. Chow, Y. Chim, J. Wang, N. Zhang, R. Wong, N. Tang, K. Leung, W. Cheung","doi":"10.22203/eCM.v038a16","DOIUrl":"https://doi.org/10.22203/eCM.v038a16","url":null,"abstract":"Fracture healing is a well-orchestrated and coordinated process and begins with the inflammatory stage involving the infiltration of immune cells and the release of cytokines, including tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). Low-magnitude high-frequency vibration (LMHFV) stimulation is effective in promoting fracture healing. The study hypothesis was that the innate immune response was impaired in osteoporotic fracture and LMHFV could positively modulate it. 9-month-old ovariectomy (OVX)-induced osteoporotic rats were randomised into sham (SHAM), OVX control (OVX), OVX-vibration (OVX-VT) or OVX vibration plus administration of COX-2 specific non-steroid anti-inflammatory drugs (OVX-VT-NSAID). LMHFV (35 Hz, 0.3 g) was given 20 min/d and 5 d/week to the treatment groups. Healing and innate immune response were evaluated by weekly radiographs, endpoint micro-computed tomography (µCT), enzyme-linked immunosorbent assay (ELISA) and histomorphometry at weeks 1, 2, 4 and 8 post-treatment. Results showed that OVX slightly elevated systemic inflammation but impaired the innate immune response locally at the fracture site, with significantly lower expressions of TNF-α and IL-6 but higher IL-10 expression during the early stage of healing. LMHFV was effective in accelerating the delayed fracture healing in OVX bones by partly restoring the impaired innate immune response at the fracture site, accompanied by promoted progression of macrophage polarisation from M1 (pro-inflammatory) to M2 (anti-inflammatory) phenotype. In conclusion, vibration treatment could positively modulate the impaired innate immune response and promote macrophage polarisation in osteoporotic-fracture healing.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"38 1","pages":"228-245"},"PeriodicalIF":3.1,"publicationDate":"2019-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22203/eCM.v038a16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48878058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Effective repair of articular cartilage using human pluripotent stem cell-derived tissue. 利用人多能干细胞衍生组织有效修复关节软骨。

IF 3.1 3区医学

European cells & materials Pub Date : 2019-11-05 DOI: 10.22203/eCM.v038a15

O F Gardner, S C Juneja, H Whetstone, Y Nartiss, J T Sieker, C Veillette, G M Keller, A M Craft

{"title":"Effective repair of articular cartilage using human pluripotent stem cell-derived tissue.","authors":"O F Gardner, S C Juneja, H Whetstone, Y Nartiss, J T Sieker, C Veillette, G M Keller, A M Craft","doi":"10.22203/eCM.v038a15","DOIUrl":"https://doi.org/10.22203/eCM.v038a15","url":null,"abstract":"In an effort to develop an effective source of clinically relevant cells and tissues for cartilage repair a directed differentiation method was used to generate articular chondrocytes and cartilage tissues from human embryonic stem cells (hESCs). It has previously been demonstrated that chondrocytes derived from hESCs retain a stable cartilage-forming phenotype following subcutaneous implantation in mice. In this report, the potential of hESC-derived articular-like cartilage to repair osteochondral defects created in the rat trochlea was evaluated. Articular cartilage-like tissues were generated from hESCs and implanted into the defects. After 6 and 12 weeks, the defects were evaluated histologically and immunohistochemically, and the quality of repair was assessed using a modified ICRS II scoring system. Following 6 and 12 weeks after implantation, hESC-derived cartilage tissues maintained their proteoglycan and type II collagen-rich matrix and scored significantly higher than control defects, which had been filled with fibrin glue alone. Implants were found to be well integrated with native host tissue at the basal and lateral surfaces, although implanted human cells and host cells remained regionally separated. A subset of implants underwent a process of remodeling similar to endochondral ossification, suggesting the potential for a single cartilaginous implant to promote the generation of new subchondral bone in addition to repair of the articular cartilage. The ability to create cartilage tissues with integrative and reparative properties from an unlimited and robust cell source represents a significant advance for cartilage repair that can be further developed in large animal models before clinical- setting application.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"38 ","pages":"215-227"},"PeriodicalIF":3.1,"publicationDate":"2019-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22203/eCM.v038a15","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9457286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products. 人牙髓干细胞与血浆衍生产物在仿生种植体表面的粘附、整合和成骨。

IF 3.1 3区医学

European cells & materials Pub Date : 2019-11-04 DOI: 10.22203/eCM.v038a14

I. Irastorza, J. Luzuriaga, R. Martínez‐Conde, G. Ibarretxe, F. Unda

{"title":"Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products.","authors":"I. Irastorza, J. Luzuriaga, R. Martínez‐Conde, G. Ibarretxe, F. Unda","doi":"10.22203/eCM.v038a14","DOIUrl":"https://doi.org/10.22203/eCM.v038a14","url":null,"abstract":"Dental implants are the usual therapy of choice in the dental clinic to replace a loss of natural teeth. Over recent decades there has been an important progress in the design and manufacturing of titanium implant surfaces with the goal of improving their osteointegration. In the present work, the aim was to evaluate the usefulness of hDPSCs (human dental pulp stem cells), in combination with autologous plasma components, for in vitro bone generation on biomimetic titanium dental implant materials. In this context, the combination of hDPSCs stimulated by PRGF or PRF and cultured on standard Ti6A14V and biomimetic BAS™ (Avinent Implant System) titanium surfaces were studied in order to evaluate possible enhancements in the osteoblastic differentiation process out of human mesenchymal cells, as well as bone matrix secretion on the implant surface. The results obtained in this in vitro model of osteogenesis suggested a combination of biomimetic rough titanium surfaces, such as BAS™, with autologous plasma-derived fibrin-clot membranes such as PRF and/or insoluble PRGF formulations, but not with an addition of water-soluble supplements of plasma-derived growth factors, to maximise osteoblastic cell differentiation, bone generation, anchorage and osteointegration of titanium-made dental implants.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"38 1","pages":"201-214"},"PeriodicalIF":3.1,"publicationDate":"2019-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22203/eCM.v038a14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46351543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15