Endocrinology最新文献_第10页

Puberty Blocker, Leuprolide, Reduces Sex Differences in Rough-and-Tumble Play and Anxiety-like Behavior in Juvenile Rats 青春期阻断剂亮丙瑞林可减少幼年大鼠粗暴翻滚游戏和焦虑行为的性别差异

IF 4.8 3区医学

Endocrinology Pub Date : 2024-04-10 DOI: 10.1210/endocr/bqae046

Gabriela de Faria Oliveira, Amber T. Nguyen, Leykza Carreras-Simons, Thomas Niepsuj, Salma H. Gadelhak, Aimee K. Johnson, Ashwakh Abdalla, Eden Lev, Sofia G. Torres Roman, Samantha N Fuchs, Joan S. Jorgensen, Walid A. Farhat, Anthony P. Auger

{"title":"Puberty Blocker, Leuprolide, Reduces Sex Differences in Rough-and-Tumble Play and Anxiety-like Behavior in Juvenile Rats","authors":"Gabriela de Faria Oliveira, Amber T. Nguyen, Leykza Carreras-Simons, Thomas Niepsuj, Salma H. Gadelhak, Aimee K. Johnson, Ashwakh Abdalla, Eden Lev, Sofia G. Torres Roman, Samantha N Fuchs, Joan S. Jorgensen, Walid A. Farhat, Anthony P. Auger","doi":"10.1210/endocr/bqae046","DOIUrl":"https://doi.org/10.1210/endocr/bqae046","url":null,"abstract":"Abstract We examined the effect of the puberty blocker, leuprolide acetate, on sex differences in juvenile rough-and-tumble play behavior and anxiety-like behavior in adolescent male and female rats. We also evaluated leuprolide treatment on gonadal and pituitary hormone levels and activity-regulated cytoskeleton-protein messenger RNA levels within the adolescent amygdala, a region important both for rough-and-tumble play and anxiety-like behavior. Our findings suggest that leuprolide treatment lowered anxiety-like behavior during adolescent development, suggesting that the maturation of gonadotropin-releasing hormone systems may be linked to increased anxiety. These data provide a potential new model to understand the emergence of increased anxiety triggered around puberty. Leuprolide also reduced masculinized levels of rough-and-tumble play behavior, lowered follicle-stimulating hormone, and produced a consistent pattern of reducing or halting sex differences of hormone levels, including testosterone, growth hormone, thyrotropin, and corticosterone levels. Therefore, leuprolide treatment not only pauses sexual development of peripheral tissues, but also reduces sex differences in hormones, brain, and behavior, allowing for better harmonization of these systems following gender-affirming hormone treatment. These data contribute to the intended use of puberty blockers in stopping sex differences from developing further with the potential benefit of lowering anxiety-like behavior.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140716453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grb7 ablation in mice improved glycemic control, enhanced insulin signaling and increased abdominal fat mass in females. 在小鼠体内消融 Grb7 可改善血糖控制、增强胰岛素信号传导并增加雌性小鼠的腹部脂肪量。

IF 4.8 3区医学

Endocrinology Pub Date : 2024-04-05 DOI: 10.1210/endocr/bqae045

Anke Vermehren-Schmaedick, Sonali Joshi, Wendy Wagoner, Mason A. Norgard, William Packwood, Parham Diba, Heike Mendez, Lev M Fedorov, Shauna Rakshe, Byung Park, Daniel L Marks, A. Grossberg, S. Luoh

{"title":"Grb7 ablation in mice improved glycemic control, enhanced insulin signaling and increased abdominal fat mass in females.","authors":"Anke Vermehren-Schmaedick, Sonali Joshi, Wendy Wagoner, Mason A. Norgard, William Packwood, Parham Diba, Heike Mendez, Lev M Fedorov, Shauna Rakshe, Byung Park, Daniel L Marks, A. Grossberg, S. Luoh","doi":"10.1210/endocr/bqae045","DOIUrl":"https://doi.org/10.1210/endocr/bqae045","url":null,"abstract":"OBJECTIVE\u0000Growth Factor Receptor Bound Protein 7 (GRB7) is a multi-domain signaling adaptor. Members of the Grb7/10/14 family, specifically Gbrb10/14, have important roles in metabolism. We ablated the Grb7 gene in mice to examine its metabolic function.\u0000\u0000\u0000METHODS\u0000Global ablation of Grb7 in FVB/NJ mice was generated. Growth, organ weight, food intake, and glucose homeostasis were measured. Insulin signaling was examined by Western blotting. Fat and lean body mass was measured by Nuclear Magnetic Resonance, and body composition after fasting or high fat diet was assessed. Energy expenditure was measured by indirect calorimetry. Expression of adiposity and lipid metabolism genes was measured by qPCR.\u0000\u0000\u0000RESULTS\u0000Grb7-null mice were viable, fertile and without obvious phenotype. Grb7 ablation improved glycemic control and displayed sensitization to insulin signaling in the liver. Grb7-null females but not males had increased gonadal white adipose tissue mass. Following a 12-week high fat diet, Grb7-null female mice gained fat body mass and developed relative insulin resistance. With fasting, there was less decrease in fat body mass in Grb7-null female mice. Female mice with Grb7 ablation had increased baseline food intake, less energy expenditure and displayed a decrease in the expression of lipolysis and adipose browning genes in gonadal white adipose tissue by transcript and protein analysis.\u0000\u0000\u0000CONCLUSIONS\u0000Our study suggests that Grb7 is a negative regulator of glycemic control. Our results reveal a role for Grb7 in female mice in the regulation of the visceral adipose tissue mass, a powerful predictor of metabolic dysfunction in obesity.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140736873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Cell Insulin Resistance Plays a Causal Role in Fat-Induced β-Cell Dysfunction In Vitro and In Vivo β细胞胰岛素抵抗在体外和体内脂肪诱导的β细胞功能障碍中起着因果作用

IF 4.8 3区医学

Endocrinology Pub Date : 2024-04-05 DOI: 10.1210/endocr/bqae044

Aleksandar Ivovic, Justin Hou Ming Yung, A. Oprescu, Filip Vlavcheski, Yusaku Mori, S. Rahman, Wenyue Ye, Judith A. Eversley, Michael B Wheeler, Minna Woo, E. Tsiani, Adria Giacca

{"title":"β-Cell Insulin Resistance Plays a Causal Role in Fat-Induced β-Cell Dysfunction In Vitro and In Vivo","authors":"Aleksandar Ivovic, Justin Hou Ming Yung, A. Oprescu, Filip Vlavcheski, Yusaku Mori, S. Rahman, Wenyue Ye, Judith A. Eversley, Michael B Wheeler, Minna Woo, E. Tsiani, Adria Giacca","doi":"10.1210/endocr/bqae044","DOIUrl":"https://doi.org/10.1210/endocr/bqae044","url":null,"abstract":"Abstract In the classical insulin target tissues of liver, muscle, and adipose tissue, chronically elevated levels of free fatty acids (FFA) impair insulin signaling. Insulin signaling molecules are also present in β-cells where they play a role in β-cell function. Therefore, inhibition of the insulin/insulin-like growth factor 1 pathway may be involved in fat-induced β-cell dysfunction. To address the role of β-cell insulin resistance in FFA-induced β-cell dysfunction we co-infused bisperoxovanadate (BPV) with oleate or olive oil for 48 hours in rats. BPV, a tyrosine phosphatase inhibitor, acts as an insulin mimetic and is devoid of any antioxidant effect that could prevent β-cell dysfunction, unlike most insulin sensitizers. Following fat infusion, rats either underwent hyperglycemic clamps for assessment of β-cell function in vivo or islets were isolated for ex vivo assessment of glucose-stimulated insulin secretion (GSIS). We also incubated islets with oleate or palmitate and BPV for in vitro assessment of GSIS and Akt (protein kinase B) phosphorylation. Next, mice with β-cell specific deletion of PTEN (phosphatase and tensin homolog; negative regulator of insulin signaling) and littermate controls were infused with oleate for 48 hours, followed by hyperglycemic clamps or ex vivo evaluation of GSIS. In rat experiments, BPV protected against fat-induced impairment of β-cell function in vivo, ex vivo, and in vitro. In mice, β-cell specific deletion of PTEN protected against oleate-induced β-cell dysfunction in vivo and ex vivo. These data support the hypothesis that β-cell insulin resistance plays a causal role in FFA-induced β-cell dysfunction.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zona Glomerulosa–Derived Klotho Modulates Aldosterone Synthase Expression in Young Female Mice 肾小球上皮细胞衍生的 Klotho 可调节年轻雌性小鼠体内醛固酮合成酶的表达

IF 4.8 3区医学

Endocrinology Pub Date : 2024-04-04 DOI: 10.1210/endocr/bqae040

A. Daryadel, Cong Tang, Ye Xie, Mirko Peitzsch, Viktoria Fisi, C. Hantel, D. Loffing‐Cueni, D. T. Breault, David Penton, Johannes Loffing, Felix Beuschlein

{"title":"Zona Glomerulosa–Derived Klotho Modulates Aldosterone Synthase Expression in Young Female Mice","authors":"A. Daryadel, Cong Tang, Ye Xie, Mirko Peitzsch, Viktoria Fisi, C. Hantel, D. Loffing‐Cueni, D. T. Breault, David Penton, Johannes Loffing, Felix Beuschlein","doi":"10.1210/endocr/bqae040","DOIUrl":"https://doi.org/10.1210/endocr/bqae040","url":null,"abstract":"Abstract Klotho plays a critical role in the regulation of ion and fluid homeostasis. A previous study reported that haplo-insufficiency of Klotho in mice results in increased aldosterone synthase (CYP11B2) expression, elevated plasma aldosterone, and high blood pressure. This phenotype was presumed to be the result of diminished Klotho expression in zona glomerulosa (zG) cells of the adrenal cortex; however, systemic effects on adrenal aldosterone production could not be ruled out. To examine whether Klotho expressed in the zG is indeed a critical regulator of aldosterone synthesis, we generated a tamoxifen-inducible, zG-specific mouse model of Klotho deficiency by crossing Klotho-flox mice with Cyp11b2-CreERT mice (zG-Kl-KO). Tamoxifen-treated Cyp11b2-CreERT animals (zG-Cre) served as controls. Rosa26-mTmG reporter mice were used for Cre-dependent lineage-marking. Two weeks after tamoxifen induction, the specificity of the zG-Cre line was verified using immunofluorescence analysis to show that GFP expression was restricted to the zG. RNA in situ hybridization revealed a 65% downregulation of Klotho messenger RNA expression in the zG of zG-Kl-KO female mice at age 12 weeks compared to control mice. Despite this significant decrease, zG-Kl-KO mice exhibited no difference in plasma aldosterone levels. However, adrenal CYP11B2 expression and the CYP11B2 promotor regulatory transcription factors, NGFIB and Nurr1, were enhanced. Together with in vitro experiments, these results suggest that zG-derived Klotho modulates Cyp11b2 but does not evoke a systemic phenotype in young adult mice on a normal diet. Further studies are required to investigate the role of adrenal Klotho on aldosterone synthesis in aged animals.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting NAD+ metabolism: Pre-clinical insights into potential cancer therapy strategies. 靶向 NAD+ 代谢：潜在癌症治疗策略的临床前见解

IF 4.8 3区医学

Endocrinology Pub Date : 2024-04-03 DOI: 10.1210/endocr/bqae043

A. Mogol, A. Z. Kaminsky, David J Dutton, Zeynep Madak Erdogan

引用次数: 0

Is There a Special Role for Ovarian Hormones in the Pathogenesis of Lobular Carcinoma? 卵巢激素在小叶癌的发病机制中是否起着特殊作用？

IF 4.8 3区医学

Endocrinology Pub Date : 2024-03-29 DOI: 10.1210/endocr/bqae031

Renée L Flaherty, George Sflomos, Cathrin Brisken

引用次数: 0

Human Seminal Extracellular Vesicles Enhance Endometrial Receptivity Through Leukemia Inhibitory Factor. 人类精液细胞外囊泡通过白血病抑制因子增强子宫内膜的接受能力

IF 4.8 3区医学

Endocrinology Pub Date : 2024-03-29 DOI: 10.1210/endocr/bqae035

Hanshu Wang, Yu Lin, Rongrong Chen, Yu Zhu, Hongqiang Wang, Shengxian Li, Lei Yu, Kaishu Zhang, Yujie Liu, Tao Jing, Fei Sun

引用次数: 0

Adiponectin Inhibits the Progression of Obesity-Associated Papillary Thyroid Carcinoma Through Autophagy. 脂联素通过自噬抑制肥胖相关性甲状腺乳头状癌的进展

IF 4.8 3区医学

Endocrinology Pub Date : 2024-03-29 DOI: 10.1210/endocr/bqae030

Changlin Li, Jiao Zhang, Gianlorenzo Dionigi, Nan Liang, Haixia Guan, Hui Sun

{"title":"Adiponectin Inhibits the Progression of Obesity-Associated Papillary Thyroid Carcinoma Through Autophagy.","authors":"Changlin Li, Jiao Zhang, Gianlorenzo Dionigi, Nan Liang, Haixia Guan, Hui Sun","doi":"10.1210/endocr/bqae030","DOIUrl":"10.1210/endocr/bqae030","url":null,"abstract":"Context: Obesity is a risk factor for the development of papillary thyroid cancer (PTC). However, the molecular mechanisms by which obesity promotes PTC are unclear.Objective: This study aims to identify adipokines that are linked to PTC progression.Methods: An adipokine antibody array was used to determine the serum levels of 40 adipokines in normal-weight and obese PTC patients. Enzyme-linked immunosorbent assay was used to determine the serum levels of adiponectin. Recombinant human adiponectin was produced by human adipose-derived stem cells and used to treat PTC cells. Cell proliferation and migration were evaluated using the CCK8 and Transwell assays. Bioinformatics analysis was used to predict mechanisms by which adiponectin affects PTC.Results: Adipokines differentially expressed between normal-weight and obese patients showed a gender-dependent pattern. Obese PTC patients had a significantly lower serum adiponectin level than normal-weight patients, especially in female individuals. Adiponectin levels were negatively correlated with aggressive features of PTC, including tumor diameter > 1 cm, extrathyroidal extension, and lymph node metastasis. Recombinant human adiponectin inhibited the proliferation and migration of human PTC cells in vitro. Bioinformatics analysis identified adiponectin receptor 2 (ADIPOR2) and the autophagy pathway as possible mediators of adiponectin function in TC. In vitro experiments confirmed that adiponectin activated autophagy in PTC cells. These findings shed new lights into the role and mechanisms of adiponectin in TC pathogenesis.Conclusion: Adiponectin is involved in development of obesity-related PTC. Adiponectin can directly inhibit thyroid cancer growth and metastasis through the autophagy pathway.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal Basis of Biological Sex Differences in Human Athletic Performance. 人类运动成绩中生物性别差异的荷尔蒙基础。

IF 4.8 3区医学

Endocrinology Pub Date : 2024-03-29 DOI: 10.1210/endocr/bqae036

Jonathon W Senefeld, Sandra K Hunter

{"title":"Hormonal Basis of Biological Sex Differences in Human Athletic Performance.","authors":"Jonathon W Senefeld, Sandra K Hunter","doi":"10.1210/endocr/bqae036","DOIUrl":"10.1210/endocr/bqae036","url":null,"abstract":"Biological sex is a primary determinant of athletic human performance involving strength, power, speed, and aerobic endurance and is more predictive of athletic performance than gender. This perspective article highlights 3 key medical and physiological insights related to recent evolving research into the sex differences in human physical performance: (1) sex and gender are not the same; (2) males and females exhibit profound differences in physical performance with males outperforming females in events and sports involving strength, power, speed, and aerobic endurance; (3) endogenous testosterone underpins sex differences in human physical performance with questions remaining on the roles of minipuberty in the sex differences in performance in prepubescent youth and the presence of the Y chromosome (SRY gene expression) in males, on athletic performance across all ages. Last, females are underrepresented as participants in biomedical research, which has led to a historical dearth of information on the mechanisms for sex differences in human physical performance and the capabilities of the female body. Collectively, greater effort and resources are needed to address the hormonal mechanisms for biological sex differences in human athletic performance before and after puberty.","PeriodicalId":11819,"journal":{"name":"Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: "The Role of GLP-1 in the Metabolic Success of Bariatric Surgery". 更正："GLP-1 在减肥手术代谢成功中的作用"。

IF 4.8 3区医学

Endocrinology Pub Date : 2024-03-29 DOI: 10.1210/endocr/bqae042

引用次数: 0