Euphytica最新文献

{"title":"Effect and identification of different allele combinations of SSIIa and Wx on rice eating and cooking quality","authors":"Siqi Zhang, Aoqiu Fu, Min Guo, Jinxuan Li, Zhiqing Tan, Tao Guo, Hua Zhou, Guili Yang","doi":"10.1007/s10681-024-03329-y","DOIUrl":"https://doi.org/10.1007/s10681-024-03329-y","url":null,"abstract":"<p>Rice eating and cooking quality (ECQ) is one of the most important qualities and it has attracted more and more interests of consumers and breeders. <i>SSIIa</i> and <i>Wx</i> are the major genes affecting rice ECQ, and several alleles of <i>SSIIa</i> and <i>Wx</i> have been identified. In this study, the effects of different allele combinations of <i>SSIIa</i> and <i>Wx</i> on rice ECQ were explored by analyzing amylose content (AC), gelatinization temperature (GT), and Rapid Viscosity Analyzer (RVA) profile properties of one natural population which is composed of 30 glutinous rice varieties and 342 non-glutinous varieties. Nine allele combinations of <i>SSIIa</i> and <i>Wx</i> were identified in all the non-glutinous varieties. The allele combination of <i>SSIIa</i>^<i>c</i>/<i>Wx</i>^<i>b</i> harbored higher BDV, lower SBV, intermediate AC, and higher GT, which means better taste quality but more cooking time. For non-glutinous rice, stepwise regression analysis showed that RVA profile properties are mainly controlled by polymorphic loci of <i>Wx</i>-Int1(G/T) and <i>Wx</i>-EX10(C/T); AC, PV, BDV, and SBV are mainly controlled by <i>Wx</i>-Int1(G/T); HPV, CPV, and CSV are mainly controlled by <i>Wx</i>-EX10(C/T); GT is mainly controlled by <i>SSIIa</i>-EX8(GC/TT). Regression equation models also confirmed that <i>SSIIa</i>^c/<i>Wx</i>^<i>b</i> had better ECQ compared to other allele combinations, indicating its potential to improve rice quality. Our results may provide useful clues for elite alleles of <i>SSIIa</i> and <i>Wx</i> for introgression to other rice varieties to improve rice ECQ.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining ability and hybrid breeding in pearl millet (Pennisetum glaucum [L.] R. Br.) for agronomic traits and resistance to Striga hermonthica","authors":"Armel Rouamba, Hussein Shimelis, Inoussa Drabo, Emmanuel Mrema, Jacob Mashilo, Learnmore Mwadzingeni","doi":"10.1007/s10681-024-03320-7","DOIUrl":"https://doi.org/10.1007/s10681-024-03320-7","url":null,"abstract":"<p>Pearl millet (<i>Pennisetum glaucum</i> [L.] R. Br., 2n = 2x = 14) is a nutrient-dense and climate-resilient crop widely cultivated in the dry regions of Africa and Asia. In Burkina Faso, the actual mean yield of the crop is &lt; 1 ton/ha compared with a potential yield of 3 tons/ha. Several constraints, including cultivar susceptibility to the noxious weed <i>Striga hermonthica</i> (Del.) Bentham (<i>Sh</i>) and severe and recurrent drought stress limit the potential productivity of the crop. Therefore, this study aimed to determine the combining ability effects and degree of heterosis for agronomic traits and resistance to <i>Sh</i> among complementary pearl millet genotypes to select promising parental lines and hybrids to develop and deploy farmer-preferred varieties. The narrow-and broad-sense heritability were relatively higher for <i>Striga</i>-resistance (≥ 70%) and low (≤ 23%) for grain yield. The general combining ability and specific combining ability ratios were less than unity for agronomic traits and <i>Striga</i> reaction indicating the predominance of non-additive gene action conditioning the assessed traits. The new experimental hybrids such as IP-11358 × ICMB177111, IP-11358 × IKMB18002, IP-10579 × ICMB177002 and IP-9242 × ICMB177002 are recommended for multi-environment evaluation and production in <i>Sh</i>-infested pearl millet cultivation agro-ecologies in Burkina Faso or similar agro-ecologies.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping of a novel recessive brown planthopper resistance gene bph46 from wild rice (Oryza nivara)","authors":"Pavneet Kaur, Kumari Neelam, Preetinder S. Sarao, Navneet S. Saini, Yashika Walia Dhir, Renu Khanna, Yogesh Vikal, Kuldeep Singh","doi":"10.1007/s10681-024-03316-3","DOIUrl":"https://doi.org/10.1007/s10681-024-03316-3","url":null,"abstract":"<p>Rice production is severely threatened by frequent outbreaks of Brown planthopper (BPH), Nilaparvata lugens (Stảl.) biotypes globally. On this account, host-plant resistance serves as an important strategy to reduce the damage caused by BPH. The wild species of rice <i>Oryza nivara</i> accession IRGC 93198 showed consistent resistance reaction against BPH biotype 4 for 5 consecutive years of screening under the greenhouse conditions. The mapping of the BPH resistance gene from <i>Oryza nivara</i> accession IRGC 93198 was conducted using BC2F2 and BC2F3 progenies. Out of 239 BC2F2 plants, 65 plants were resistant (1-3 score), and 174 plants (5, 7, and 9 score) were susceptible, thus fitting the segregation ratio of 3:1 (Susceptible: Resistant). The BC2F3 progenies segregated in 1:2:1 confirming that the resistance from <i>O. nivara</i> is governed by a single recessive gene. Bulked segregant analysis (BSA) identified genomic region on the short arm of chromosome 4 to be associated with BPH resistance. Molecular mapping performed on BC2F2 population identified a QTL on chromosome 4 within the marker interval RM16285 and RM6314 explaining phenotypic variance of 27% at LOD 22.34. The linked marker RM6659 was found efficient in demarcating the susceptible from resistant lines when applied on the panel of rice cultivars, hence can be used for marker assisted selection in crop breeding. The previously identified BPH-resistant genes located on chromosome 4 were found susceptible to the BPH biotype 4 screening test. This specifies bph46 to be a novel gene that can be deployed as a valuable donor in BPH resistance breeding programs.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140299086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marker-assisted gene introgression for resistance to Xanthomonas oryzae pv. oryzae in rice for the control of bacterial leaf blight","authors":"Md. Ariful Islam, Md. Moniruzzaman Hasan, Md. Ataur Rahman, Tanbin Akter, Md. Ashraful Haque","doi":"10.1007/s10681-024-03331-4","DOIUrl":"https://doi.org/10.1007/s10681-024-03331-4","url":null,"abstract":"<p>Bacterial leaf blight, sometimes known as BLB, is one of the most damaging diseases that may jeopardize the world’s supply of rice. It is caused by the bacterium <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). It has caused sharp decline in production in regions of the globe that produce rice. More than 40 previously characterized resistance (R) genes enable host tolerance for diverse <i>Xoo</i> strains. In this study, three BLB resistant genes, including <i>xa5</i>, <i>xa13</i>, and <i>Xa21</i>, which have been crucial in disease prevention in Bangladesh, were introgressed into populations. These populations were created by crossing IRRI 154, a popular rice variety with a modern genetic background, with IRBB66 (resistant to BLB). Fifteen virulent bacterial isolates were used for BLB infection, and promising recombinants from the F₅ and BC₂F₄ generations were found to be resistant. Using marker assisted selection (MAS) with gene-specific primers on generations F₅ and BC₂F₄, we were able to find that 60 recombinant introgressed lines (RILs) had a pyramiding of BLB resistance genes. In terms of agronomic performance, the RILs outperformed both their donor and recipient parents, demonstrating that the RILs had pyramided three resistance genes, therefore conferring broad-spectrum BLB resistance. The produced BLB-resistant RILs offer substantial future development potential, either as cultivable crops or as BLB resistance donor material for use in boosting the yields of other rice lines.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140199729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biometrics and screening of kale (Brassica oleracea var. acephala) half-sib progenies obtained by recurrent selection in Brazil","authors":"Natália Oliveira Silva, Valter Carvalho de Andrade Júnior, Orlando Gonçalves Brito, Amanda Gonçalves Guimarães, José Sebastião Cunha Fernandes, Alcinei Mistico Azevedo, Marcelo Augusto Vieira Santos, Ariana Lemes da Costa","doi":"10.1007/s10681-024-03319-0","DOIUrl":"https://doi.org/10.1007/s10681-024-03319-0","url":null,"abstract":"<p>Kale is one of the main leafy vegetables grown in Brazil. However, genetic breeding programs for the development of superior cultivars are scarce. Thus, the objective of this study was to select promising genotypes and to obtain strategic information for the genetic improvement of kale from plant biometrics. The study was conducted in two stages in the horticulture sector of Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) during 2018 and 2019. The university is located in the municipality of Diamantina, state of Minas Gerais, Brazil. The seeds used were obtained from 25 pre-selected half-sib progenies. In 2018, these progenies were recombined, selecting the seeds from plants with superior agronomic performance. Subsequently, in the year 2019, the seeds harvested in the first stage were sown, and the agronomic evaluation of the resulting plants was conducted. The experimental design was randomized blocks with four replicates and six plants per plot. At this stage, agronomic biometrics of the plants were conducted, identifying those with higher productivity and leaf quality. The genetic parameters were estimated, and the genetic gains with selection were predicted. The highest individual heritability in the narrow sense (h²_a) was observed for plant height, followed by the mean mass per leaf. The yield of leaves showed gains of 11.80, 10.39, and 7.68% at selection intensities of 10, 15, and 30%, respectively, indicating the possibility of genetic progress with the selection of superior genotypes. The individual selection approach proved effective in improving the population, however, indirect selection for increased leaf number resulted in a reduction in leaf size.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140199807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study reveals the genetic basis of cold tolerance in soybean","authors":"Zhiyu Wang, Wei Li, Yaning Gao, Ming Shao, Kaiyi Yin, Yixiang Pu, Hao Cheng, Deyue Yu, Fang Huang, Hengyou Zhang, Jiao Wang","doi":"10.1007/s10681-024-03311-8","DOIUrl":"https://doi.org/10.1007/s10681-024-03311-8","url":null,"abstract":"<p>Soybean [<i>Glycine max</i> (L.) Merr.] is an important seed crop with agricultural sustainability that can be used to meet the growing demand for plant-based oil and protein worldwide. Expanding the cultivation of soybean to high-latitude regions could be an effective strategy for enhancing soybean production. Low temperatures in these regions can inhibit seed germination during the planting season. Despite their importance, many cold tolerance-related genes in soybean remain unidentified. Here, we carried out a genome-wide association study (GWAS) in a diverse soybean population with large variations in cold tolerance-related germination traits. The analyses led to the identification of a total of 46 single-nucleotide polymorphisms (SNPs) that were significantly associated with cold tolerance-related traits. We identified a new locus on chromosome 18, <i>qCold-18–3</i>, that was associated with both the low-temperature germination rate and potential. Furthermore, <i>Abscisic Acid-Deficient4</i> (<i>GmABA4</i>) was prioritized as the most promising candidate gene. In addition, <i>GmABA4</i> was strongly induced in a cold-tolerant accession by cold stress during germination, and it contains <i>cis</i>-acting elements associated with low temperature in its promoter region. Haplotype analysis revealed that the accessions harboring Hap3 of <i>GmABA4</i> were more tolerant to low temperature than those harboring the other haplotypes. In this study, a new cold tolerance-related candidate gene, <i>GmABA4,</i> was identified through GWAS, and further study could advance our understanding of the underlying genetic mechanisms and facilitate the breeding of soybean with improved cold tolerance.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140168706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine mapping and identification of regulatory genes for fruit neck length in bottle gourd (Lagenaria siceraria)","authors":"Gui Zhang, Yulai Huo, Peng Wang, Wenjin Yu, Mujie Shen, Rongchong Zhao, Yi Chen, Kunfeng Li, Yanjuan Yang","doi":"10.1007/s10681-024-03318-1","DOIUrl":"https://doi.org/10.1007/s10681-024-03318-1","url":null,"abstract":"<p>Fruit neck length (FNL) is one of the important commercial characteristics of bottle gourd (<i>Lagenaria siceraria</i>), which is usually desirable by consumers based on their preferences. At present, there has not been extensive research on the genetics of bottle gourd FNL. In this study, an F₂ segregating population was constructed using the long fruit neck material H16 and the short fruit neck material H06. Bulked segregant analysis and kompetitive allele-specific PCR techniques were used to identify candidate regions regulating FNL in bottle gourd. InDel markers were used to narrow the final candidate region interval to 0.16 Mb, which contained 8 genes. A combined analysis using a gene annotation library, real-time fluorescence quantitative expression, and sequence analysis of candidate genes in the region indicated that the <i>HG_GLEAN_10011965</i> gene might be involved in the regulation of bottle gourd FNL and was named <i>LsFNL1.1</i>. Based on the most narrowed candidate gene interval, an InDel marker was developed for molecular marker assisted breeding of bottle gourd FNL. The phenotypic-genotype association rate of this InDel marker was 83%. This study provides a theoretical basis for further exploring the molecular mechanisms underlying bottle gourd fruit traits and can accelerate the breeding of varieties with desirable characteristics.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140168563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening tropical and sub-tropical maize germplasm for resistance to Striga hermonthica and S. asiatica and yield-related traits","authors":"Emeline N. Dossa, Hussein Shimelis, Admire I. T. Shayanowako, Mark D. Laing","doi":"10.1007/s10681-024-03309-2","DOIUrl":"https://doi.org/10.1007/s10681-024-03309-2","url":null,"abstract":"<p>Identification of maize germplasm with dual resistance to <i>Striga hermonthica (Sh)</i> and <i>S. asiatica (Sa)</i>, could lead to the development of cultivars with stable resistance. 130 tropical and sub-tropical maize germplasms, including checks, were evaluated in a controlled environment for their reaction to <i>Sh</i> and <i>Sa</i> infestations using a 13 × 10 alpha lattice design with two replications over two seasons. Significant differences (<i>P</i> &lt; 0.05) were detected among the assessed genotypes for all the recorded traits in <i>Sh</i> and <i>Sa</i>-infested treatments. Under <i>Sa</i>-infested conditions, mean <i>Striga</i> emergence counts 8 weeks after planting (SEC8) and 10 weeks after planting (SEC10) were 5.00 and 45.50, respectively, while the mean <i>Striga</i> damage rate 8 weeks after planting (SDR8) and 10 weeks after planting (SDR10) were 3.35 and 3.07, respectively. Under <i>Sh</i>-infested conditions, SEC8 and SEC10 mean values were 3.66 and 3.77, respectively, while the SDR8 and SDR10 values were 5.25 and 2.75 respectively. Positive and significant (<i>P</i> &lt; 0.05) correlations were found between anthesis-silking interval (ASI) and SDR8 (r = 0.18) and SDR10 (0.32) under <i>Sa</i>-infested conditions. Negative and significant correlations were recorded between ear per plant (EPP) and SEC8, SDR8, and SDR10, with r = − 0.18, r = − 0.27, and r = − 0.24, respectively. Under <i>Sh</i>-infested conditions, significant and negative correlations were recorded between SDR8 and EPP (r = − 0. 20), EHT and SEC8 (r = − 0.22), EHT and SDR8 (r = − 0.36), PLHT and SDR8 (− 0.48), and PLHT and SDR10 (− 0.22). The results suggest that dual resistance to the two <i>Striga</i> species exists in some tropical and sub-tropical maize lines. The following genotypes have dual resistance to <i>Sa</i> and <i>Sh</i>: CML440, CML566, CML540, CML539, CLHP0343, CLHP0326, TZISTR1248, TZSTRI115, TZISTR25, TZISTR1205, TZSTRI113, TZISTR1119, TZISTR1174 and the OPVs B.King/1421, Shesha/1421, ZM1421, DTSTR-W SYN13, DTSTR-Y SYN14, and 2*TZECOMP3DT/WhiteDTSTRSYN) C2. The identified genotypes are suitable for use as parents in developing high-performing maize varieties with <i>Striga</i> resistance and improved grain yield.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140154513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a SNP-based linkage map and identification of QTLs for woody biomass-related traits using an interspecific F2 population derived from Jatropha curcas ×  Jatropha integerrima","authors":"Kularb Laosatit, Kitiya Amkul, Prakit Somta, Taeyoung Lee, Sangrea Shim, Suk-Ha Lee, Peerasak Srinives","doi":"10.1007/s10681-024-03306-5","DOIUrl":"https://doi.org/10.1007/s10681-024-03306-5","url":null,"abstract":"<p><i>Jatropha curcas</i> (known as jatropha and physic nut) can be used as an alternative source for biodiesel and biomass production. However, conventional breeding of <i>J. curcas</i> needs time, labor and land for characterization and evaluation of the progenies owing to its perennial growth habit. Quantitative trait loci (QTL) mapping can help identifying marker-trait association and accelerate breeding process of perennial crops through marker-assisted selection. However, the genetic basis for woody biomass improvement in jatropha has not yet been studied. In this manuscript, we report the construction of a SNP-based linkage map and detection of QTLs for woody-biomass-related traits using an F₂ population of the interspecific cross between <i>J. curcas</i> and <i>Jatropha</i> <i>integerrima</i>. A high-density genetic linkage map containing 2,179 SNPs was constructed. As high as 89.26% of the SNP markers developed from these interspecific progenies expressed significant segregation distortion. Most of the distorted markers showed neither gametophytic nor zygotic selection, except those on linkage groups 3, 5 and 6 which showed gametophytic and zygotic selection. The F₂ population showed high variation and transgressive segregation for plant height, canopy width and wood density. QTL analyses detected 5, 8, and 7 loci for plant height, canopy width, and wood density, respectively. The SNPs linked to these QTLs could be incorporated into marker-assisted breeding to maximize the selection gain in jatropha biomass breeding.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140126262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping for yield related traits in rice reveals major effect QTL qFLA1.1 from Oryza nivara increases flag leaf area","authors":"Haritha Guttikonda, Gowthami Chandu, Suchandranath Babu Munnam, Kavitha Beerelli, Divya Balakrishnan, R. Madhusudhana, Sarla Neelamraju","doi":"10.1007/s10681-024-03297-3","DOIUrl":"https://doi.org/10.1007/s10681-024-03297-3","url":null,"abstract":"<p>A stable back cross introgression line IL65 (IET22161) (Swarna/<i>O. nivara-</i>BC₂F₆) of rice was used to map flag leaf related traits in F₂ and F₃ populations. A total of 12 QTLs were mapped on two chromosomes with each QTL explaining 3 to 21% phenotypic variance (PV). Interestingly, a novel 12 Mb QTL cluster (RM8094—RM9) that controls 7 traits was identified on long arm of chromosome 1 where QTLs <i>qSPAD1.2</i>, <i>qSPAD1.3</i> for SPAD, <i>qFLL1.1</i>, <i>qFLL1.2</i> for flag leaf length, <i>qFLW1.1</i>, <i>qFLW1.2</i> for flag leaf width, <i>qFLA1.1</i>, <i>qFLA1.2</i> for flag leaf area, <i>qPH1.1</i>, <i>qPH1.2</i> for plant height, <i>qDTF1.2</i>, <i>qDTF1.3</i> for days to flowering and <i>qHI1.2</i>, <i>qHI1.3</i> for harvest index were co-located. Among these, one major effect QTL <i>qFLA1.1</i> for flag leaf area explaining 12.7% PV was identified in a 9 Mb region between RM8094 and RM5638. There was an adjacent minor effect QTL <i>qFLA1.2</i> with 7% PV in a 3 Mb region between RM5638 and RM9. Together, these two QTLs from <i>O. nivara</i> explained 19.7% PV of leaf area. The QTL for flag leaf related traits can be fine mapped and considered for breeding rice varieties with higher flag leaf area, photosynthetic rate and grain yield.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140115947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}