Equine veterinary journal. Supplement最新文献

{"title":"Dedication to Professor G. Barrie Edwards.","authors":"T S Mair, C J Proudman","doi":"10.1111/j.2042-3306.2011.00436.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00436.x","url":null,"abstract":"","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"v-vi"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00436.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of mechanical manipulations on the local inflammatory reaction in the equine colon.","authors":"C Hopster-Iversen,&nbsp;K Hopster,&nbsp;C Staszyk,&nbsp;K Rohn,&nbsp;D Freeman,&nbsp;A K Rötting","doi":"10.1111/j.2042-3306.2011.00378.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00378.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Large intestinal diseases in horses are characterised by inflammation, which could arise from the disease process with some contribution from intestinal manipulation. The effects of the latter are unknown but important to surgeons and could contribute to post operative complications.</p><p><strong>Objectives: </strong>To characterise type and degree of intestinal inflammation induced by various mechanical stimuli in the equine ascending colon.</p><p><strong>Methods: </strong>Laparotomy was performed in 12 horses, the left dorsal colon exteriorised and 3 segments randomly exposed to different mechanical manipulations: 1) enterotomy, 2) enterotomy and mucosal irritation and 3) serosal irritation. Intestinal biopsies were harvested before, immediately after and 30 min after each manipulation for histological evaluation. Eosinophils were detected with Luna's stain and neutrophils identified by immunohistochemical staining for calprotectin. Additionally, left dorsal colon samples from 14 horses from a jejunal ischaemia-reperfusion study were collected immediately after laparotomy (7 horses) and at the end of the experiment without previous manipulation of the colon (7 horses). Horses were subjected to euthanasia at the end of both studies.</p><p><strong>Results: </strong>Redistribution of mucosal neutrophils and eosinophils towards the luminal surface and increased neutrophilic infiltration of the submucosa were demonstrated after serosal and mucosal irritation. All manipulations resulted in serosal infiltration with neutrophils. Laparotomy and small intestinal manipulation increased mucosal eosinophilic infiltration.</p><p><strong>Conclusions and potential relevance: </strong>Mechanical intestinal manipulation caused a rapid local inflammatory reaction in the mucosa, submucosa and serosa including a mucosal eosinophilic response. These changes could exacerbate existing inflammation in horses with large colon disease. Colic surgery can lead to intestinal inflammation in nonmanipulated intestine and this could contribute to a higher morbidity rate in horses after prolonged colic surgery. An intestinal biopsy should be collected at the beginning of surgery to avoid false interpretations.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00378.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nephrosplenic entrapment of the large colon in 142 horses (2000-2009): analysis of factors associated with decision of treatment and short-term survival.","authors":"C Lindegaard,&nbsp;C T Ekstrøm,&nbsp;S B Wulf,&nbsp;J M B Vendelbo,&nbsp;P H Andersen","doi":"10.1111/j.2042-3306.2011.00376.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00376.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Previous studies indicate similar overall survival of horses with nephrosplenic entrapment of the large colon (NSE), regardless of treatment strategy. Short-term survival of a primarily conservative treatment strategy without rolling under general anaesthesia (GA) and a low proportion of surgical intervention as well as indicators of short-term nonsurvival has not been documented.</p><p><strong>Objectives: </strong>To document short-term survival of horses with NSE treated in a university referral hospital with a low rate of surgical interventions and to determine factors associated with the decision of treatment and short-term nonsurvival.</p><p><strong>Methods: </strong>A retrospective review of medical records of 142 horses diagnosed with NSE between January 2000 and October 2009 was undertaken. Case details and clinical parameters from the initial examination, treatment and outcome were recorded. Factors associated with decision of treatment and short-term survival were identified by multiple logistic regression analysis.</p><p><strong>Results: </strong>Warmblood breeds were over-represented in comparison to the general colic population. Overall short-term survival was 91.5% (130/142) which is similar to previous studies. Three horses considered to be in need of surgery were subjected to euthanasia for economical reasons before treatment. Of 114 conservatively treated horses, 110 (96.5%) survived, as did 20/25 (80%) of surgically treated horses. Nine conservatively managed horses were treated with phenylephrine. Gastric reflux (P = 0.0077), pain (P = 0.024) and abdominal distension (P = 0.05) were associated with the decision to treat surgically. Increased heart rate (P<0.001), and surgery (P = 0.032) were associated with reduced likelihood of short-term survival.</p><p><strong>Conclusions and potential relevance: </strong>Overall short-term survival was similar to that reported in previous studies with higher proportions of surgically managed cases. Consequently, horses with NSE should be managed by a primarily conservative treatment strategy, with the decision to treat surgically based on specific evidence based criteria.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00376.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent colic in the horse: incidence and risk factors for recurrence in the general practice population.","authors":"C E Scantlebury,&nbsp;D C Archer,&nbsp;C J Proudman,&nbsp;G L Pinchbeck","doi":"10.1111/j.2042-3306.2011.00383.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00383.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>The frequency of recurrent colic in the UK equine general practice population is previously unreported. Elucidating risk factors for recurrent colic could provide a basis for clinical decision making and interventions.</p><p><strong>Objectives: </strong>To determine the incidence rate of and risk factors for recurrent colic.</p><p><strong>Hypotheses: </strong>Horse management, prophylactic health care and innate behaviour contribute to the risk of recurrent colic.</p><p><strong>Methods: </strong>A cohort of 127 horses was enrolled at the point of a veterinary-diagnosed medical colic episode. Participating owners completed a baseline and 3 follow-up telephone questionnaires over one year. Clinical details of each colic episode were collected with data on management, behaviour and preventive healthcare. Incidence was calculated using time at risk data; non-time varying covariates were assessed for association with recurrent colic using multivariable logistic regression.</p><p><strong>Results: </strong>The recurrence rate was 50 colic events/100 horse years at risk (HYAR). Including only veterinary attended recurrent colic episodes the incidence was 35 colic events per 100 HYAR. A multivariable logistic regression model was built to explore non-time varying risk factors for recurrence collected from baseline data. The model showed that horses that have a known dental problem (OR 5.5, 95% CI 1.3, 23.1) or crib-bite/windsuck (OR 12.1, 95% CI 1.4, 108.1) were at increased risk of recurrence during the year following a colic event.</p><p><strong>Conclusions and potential relevance: </strong>The incidence of recurrence in horses following a medical episode of colic is high in this population and represents a welfare concern. The incidence rate can be used to compare intervention efficacy in similar populations. Identified risk factors could provide the basis for management interventions or highlight at risk individuals.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"81-8"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00383.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sedation on D(+)-xylose absorption tests in 6 normal horses.","authors":"C Fintl,&nbsp;C F Ihler","doi":"10.1111/j.2042-3306.2011.00374.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00374.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>D(+)-xylose absorption tests are commonly performed when investigating suspected small intestinal malabsorption in the horse. The test involves the administration of a D(+)-xylose solution via a nasogastric tube followed by serial blood sampling to determine its rate of absorption. In some horses, nasogastric intubation cannot be safely performed without prior administration of a sedative. Due to its short duration of action, the α(2) agonist xylazine is commonly used for this purpose. However, α(2) agonists have also been reported to influence the rate of gastric emptying as well as small intestinal motility patterns.</p><p><strong>Objective: </strong>To evaluate if prior sedation with xylazine would influence the rate of absorption of D(+)-xylose in 6 normal Standardbred horses in a randomised cross-over study.</p><p><strong>Methods: </strong>D(+)-xylose was administered by nasogastric intubation at a dose rate of 0.5 g/kg bwt given as a 10% solution with water while xylazine was administered iv at a dose rate of 0.5 mg/kg bwt. A heparinised blood sample was collected prior to administration of D(+)-xylose (and xylazine when used) and then at 30, 45, 60, 75, 90, 120, 150, 180 and 240 min following administration. Samples were immediately analysed using a modified colorimetric micro method. The cumulative amount of D(+)-xylose absorbed at each time point with and without prior sedation were. The significance rate was set at P<0.05.</p><p><strong>Results: </strong>The study failed to demonstrate a statistically significant difference in the amount of D(+)-xylose absorbed between sedated and unsedated animals, although there was a tendency for a less rapid initial uptake with prior sedation.</p><p><strong>Conclusion: </strong>This study suggests that prior sedation with xylazine will not significantly affect the result of a D(+)-xylose absorption test in the normal horse.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"149-52"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00374.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30034318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic evidence of colonic mesenteric vessels as an indicator of right dorsal displacement of the large colon in 13 horses.","authors":"N S Grenager,&nbsp;M G Durham","doi":"10.1111/j.2042-3306.2011.00418.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00418.x","url":null,"abstract":"<p><p>This report describes the use of ultrasound to diagnose right dorsal displacement of the large colon (RDDLC) in 13 horses prior to surgery. Horses had ultrasonographic examinations performed of the right lateroventral aspect of the abdomen upon admission to the hospital with a 2-5 MHz broadband curvilinear sector scanning transducer after alcohol was used to wet the hair. First, the caecal vessels were identified in the right flank and followed medially and cranially. Next, each intercostal space, from caudal to cranial, was scanned from dorsal to ventral evaluating for abnormally-located mesenteric vessels associated with the large colon. Abnormally-located mesenteric vessels associated with the large colon, distinct from the caecal vessels, were identified in 13 of 23 horses with a diagnosis of RDDLC obtained at exploratory laparotomy. In horses, ultrasonographic visualisation of mesenteric vessels along the right lateral abdomen, dorsal to the costochondral junction in at least 2 intercostal spaces, distinct from the caecal vessels, is consistent with a surgical diagnosis of RDDLC.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"153-5"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00418.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30034319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strongyle egg counts in Standardbred trotters: are they associated with race performance?","authors":"P Fog,&nbsp;H Vigre,&nbsp;M K Nielsen","doi":"10.1111/j.2042-3306.2011.00381.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00381.x","url":null,"abstract":"<p><strong>Reasons for performing study: </strong>Strongyle worm burdens are assumed to subclinically affect equine performance. This assumption appears to be particularly pronounced in the equine racing industry.</p><p><strong>Hypothesis: </strong>Race results of Standardbred trotters are negatively affected by high strongyle faecal egg count levels.</p><p><strong>Methods: </strong>Faecal samples were obtained from 213 racing Standardbred trotters, aged ≥ 2 years, and stabled at training facilities of 21 professional trainers with license at racecourses in Denmark. Strongyle egg counts were generated using a McMaster technique. Race results were recorded as the finishing position of the horse (position 1-3 vs. finishing lower) and winning purse. The effect of strongyle egg counts on performance was assessed using regression analyses.</p><p><strong>Results: </strong>Strongyle egg counts ranged from 0-3500 with a mean of 319 and a median of 150 eggs/g. Finishing in positions 1-3 was significantly associated with higher egg counts.</p><p><strong>Conclusions: </strong>Race performance of the population of professionally trained Danish Standardbred trotters was not negatively affected by higher strongyle faecal egg count levels.</p><p><strong>Potential relevance: </strong>The traditional frequent anthelmintic treatments of racehorses may be inordinate.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"89-92"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00381.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there a statistical correlation between ultrasonographic findings and definitive diagnosis in horses with acute abdominal pain?","authors":"F Beccati,&nbsp;M Pepe,&nbsp;R Gialletti,&nbsp;M Cercone,&nbsp;C Bazzica,&nbsp;S Nannarone","doi":"10.1111/j.2042-3306.2011.00428.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00428.x","url":null,"abstract":"<p><strong>Reason for performing study: </strong>Abdominal ultrasonography has become a part of the diagnostic investigation for the acute abdomen in many equine clinics. There is limited information on the correlation between abnormalities detected on abdominal ultrasonography and the specific category of small intestine (SI) and large intestine (LI) diseases.</p><p><strong>Objectives: </strong>To investigate the relationship between abdominal ultrasonographic findings and disease categories that cause abdominal pain requiring surgery.</p><p><strong>Methods: </strong>Medical records were reviewed for horses undergoing surgery or post mortem examination for colic. The ultrasound examination was performed to assess free peritoneal fluid, the left kidney, stomach, appearance and motility of the duodenum, identification, appearance, motility and thickness of small intestine loops, and the appearance and motility of the colon. Logistic regression analysis was used to identify associations between disease categories and ultrasonographic findings; a Chi-squared test was used to test for associations between each variable and disease categories.</p><p><strong>Results: </strong>The study included 158 horses. Distended and nonmotile SI loops were associated with strangulated obstruction (n = 45); increased free peritoneal fluid, completely distended SI loops with abnormal motility and thickened loops were associated with definitive diagnosis involving SI (n = 58). Failure to visualise the left kidney was associated with renosplenic entrapment (n = 16); thickened large colon (LC) was associated with LC strangulating volvulus (n = 9).</p><p><strong>Conclusion: </strong>The use of abdominal ultrasonography can be used for the accurate definitive diagnosis involving SI and LI diseases.</p><p><strong>Potential relevance: </strong>This retrospective study may be used as a basis for prospective studies to assess the ultrasonographic findings in horses with medical colic and to compare these with surgical findings.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"98-105"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00428.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29888306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural changes in the equine colonic mucosa after ischaemia and reperfusion.","authors":"A Grosche,&nbsp;A J Morton,&nbsp;A S Graham,&nbsp;L C Sanchez,&nbsp;A T Blikslager,&nbsp;M M R Polyak,&nbsp;D E Freeman","doi":"10.1111/j.2042-3306.2011.00402.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00402.x","url":null,"abstract":"<p><strong>Reason for performing study: </strong>Ultrastructural changes in the epithelium can provide information on early changes in barrier properties, repair and inflammation in equine colon after ischaemia and reperfusion (I/R).</p><p><strong>Objectives: </strong>To describe the morphology and ultrastructure of the epithelium in equine large colonic mucosa after I/R, and the response of inflammatory cells to injury.</p><p><strong>Methods: </strong>Ischaemia was induced for 1 h followed by 4 h of reperfusion in a 40 cm segment of the pelvic flexure in 6 horses. Mucosal biopsies before and after ischaemia, and after 1, 2 and 4 h of reperfusion were fixed in glutaraldehyde/paraformaldehyde and osmium tetroxide, and embedded in epon. Morphological and ultrastructural changes were evaluated in toluidine blue-stained semithin sections by light microscopy and in thin sections stained with uranyl acetate/lead citrate by transmission electron microscopy.</p><p><strong>Results: </strong>Ischaemia caused swelling of epithelial cells and their organelles, opening of tight junctions, detachment from the basement membrane, early apoptosis and single cell necrosis. Autophagy was a prominent feature in epithelial cells after ischaemia. Reperfusion was characterised by apoptosis, epithelial regeneration and restoration of apical cell junctions. Phagocytic-like vacuoles containing cellular debris and bacteria were evident in epithelial cells after reperfusion. Paracellular and subepithelial clefts formed, accompanied by infiltration of neutrophils, lymphocytes and eosinophils into the epithelium. Subepithelial macrophages and luminal neutrophils had increased phagocytic activity.</p><p><strong>Conclusions: </strong>Ischaemia caused ultrastructural damage to the colonic epithelium, but epithelial cells recovered during reperfusion.</p><p><strong>Potential relevance: </strong>Transmission electron microscopy can demonstrate subtle ultrastructural damage to epithelial cells and evidence of recovery after I/R in equine colon.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00402.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucosal injury and inflammatory cells in response to brief ischaemia and reperfusion in the equine large colon.","authors":"A Grosche,&nbsp;A J Morton,&nbsp;A S Graham,&nbsp;J F Valentine,&nbsp;J R Abbott,&nbsp;M M R Polyak,&nbsp;D E Freeman","doi":"10.1111/j.2042-3306.2011.00415.x","DOIUrl":"https://doi.org/10.1111/j.2042-3306.2011.00415.x","url":null,"abstract":"<p><strong>Reason for performing study: </strong>Intestinal ischaemia and reperfusion (I/R) can activate inflammatory cells in the equine colon, although effects on different types of inflammatory cells have received little attention.</p><p><strong>Objectives: </strong>To assess early mucosal injury, the reaction of mucosal neutrophils, eosinophils, mast cells and macrophages, and cyclooxygenase (COX)-1 and -2 expression in response to I/R in the equine large colon.</p><p><strong>Methods: </strong>Large colon ischaemia was induced for 1 h (1hI) followed by 4 h of reperfusion in 6 horses, and mucosal biopsies were sampled before and after ischaemia, and after 1, 2 and 4 h of reperfusion. Semithin sections (500 nm) of epon-embedded biopsies were stained with toluidine blue for histomorphometric evaluation. The number and distribution of mucosal macrophages (CD163), neutrophils (calprotectin), eosinophils (LUNA) and mast cells (toluidine blue) were determined, and mucosal COX-1 and -2 expression was identified.</p><p><strong>Results: </strong>Ischaemia caused epithelial cell and nuclear swelling (mean ± s.e. nuclear width; control: 2.7 ± 0.2 µm vs. 1hI: 4.2 ± 0.2 µm; P<0.01), subepithelial oedema (control: 0.2 ± 0.1 µm vs. 1hI: 3.2 ± 0.2 µm; P<0.01) and increased epithelial apoptosis (control: 14.3 ± 4.1 apoptotic cells/mm mucosa vs. 1hI: 60.4 ± 14.0 apoptotic cells/mm mucosa; P<0.01). COX-2 expression (P<0.01) was evident after ischaemia. Reperfusion caused paracellular fluid accumulation (control: 0.9 ± 0.1 µm vs. 1hI: 0.6 ± 0.6 µm vs. 1hI + 4hR: 1.6 ± 0.2 µm; P<0.05). Epithelial repair started at 1 h of reperfusion (P<0.001), followed by migration of neutrophils into the mucosa after 2 h (control: 72.3 ± 18.4 cells/mm(2) mucosa vs. 1hI + 2hR: 1149.9 ± 220.6 cells/mm(2) mucosa; P<0.01). Mucosal eosinophils, mast cells and macrophages did not increase in numbers but were activated.</p><p><strong>Conclusions: </strong>Epithelial injury and COX-2 expression caused by short-term hypoxia were followed by intense inflammation associated with epithelial repair during reperfusion.</p><p><strong>Potential relevance: </strong>Equine colonic mucosa subjected to a brief period of ischaemia can repair during reperfusion, despite increased mucosal inflammation.</p>","PeriodicalId":11801,"journal":{"name":"Equine veterinary journal. Supplement","volume":" 39","pages":"16-25"},"PeriodicalIF":0.0,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.2042-3306.2011.00415.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30033903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}