European Journal of Biological Research最新文献_第4页

Pectin coating of titanium and polystyrene surfaces modulates the macrophage inflammatory response 钛和聚苯乙烯表面的果胶涂层调节巨噬细胞的炎症反应

European Journal of Biological Research Pub Date : 2018-05-21 DOI: 10.5281/ZENODO.1250541

A. Mieszkowska, J. Folkert, B. Burke, O. Addison, K. Gurzawska

{"title":"Pectin coating of titanium and polystyrene surfaces modulates the macrophage inflammatory response","authors":"A. Mieszkowska, J. Folkert, B. Burke, O. Addison, K. Gurzawska","doi":"10.5281/ZENODO.1250541","DOIUrl":"https://doi.org/10.5281/ZENODO.1250541","url":null,"abstract":"Titanium has been used with success for bone anchoring of dental implants. However, when implant surfaces are exposed to the oral environment, the progression of peri-implantitis triggered by specific oral bacteria has been reported. Bacterial colonization of implants leads to prolonged immune cell activation and bone resorption. A new strategy to improve implant biocompatibility and prevent peri-implantitis is to develop pectin surface nanocoatings. These plant-derived polysaccharides are promising candidates for surface nanocoatings of titanium implants due to their osteogenic and anti-inflammatory properties. Therefore, the aim of the study was to evaluate the in vitro effect of nanocoating with plant-derived rhamnogalacturonan-I (RG-I) on pro- and anti-inflammatory responses of primary human monocyte-derived macrophages (HMDMs) induced by Escherichia coli LPS and Porphyromonas gingivalis bacteria. In the present study, two different types of surface materials, tissue culture polystyrene (TCPS) plates and titanium (Ti) discs, coated with pectic polysaccharides, potato unmodified RG-I (PU) and potato dearabinanated RG-I (PA), have been examined. The inflammatory responses of HMDMs after E. coli LPS/P. gingivalis stimulation were investigated through gene expression measurements of pro- and anti-inflammatory cytokines. The results showed that PU and PA decreased expression of the proinflammatory genes tumour necrosis factor-alpha (TNFA), interleukin-1 beta (IL1B) and interleukin-8 (IL8) in activated HMDMs cultured on TCPS/Ti surfaces. In contrast, the effects on anti-inflammatory interleukin-10 (IL10) gene expression were not significant. The results indicate that RG-Is should be considered as a candidate for organic nanocoatings of titanium implant surfaces in order to limit host proinflammatory responses and improve bone healing. \u0000DOI: http://dx.doi.org/10.5281/zenodo.1250541","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86371195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimization of kojic acid production conditions from cane molasses using Plackett-Burman design 用Plackett-Burman设计优化甘蔗糖蜜生产曲酸的工艺条件

European Journal of Biological Research Pub Date : 2018-03-22 DOI: 10.5281/ZENODO.1211517

A. A. Zohri, G. Mahmoud, N. H. Saddek, Radwa A. Hanafy

{"title":"Optimization of kojic acid production conditions from cane molasses using Plackett-Burman design","authors":"A. A. Zohri, G. Mahmoud, N. H. Saddek, Radwa A. Hanafy","doi":"10.5281/ZENODO.1211517","DOIUrl":"https://doi.org/10.5281/ZENODO.1211517","url":null,"abstract":"Fungal synthesis of kojic acid has gained more interest in these days as an alternative way to chemical synthetic. The aspect of the microbial fermentation process is to develop a suitable culture medium to obtain the maximum amount of kojic acid using statistical methods. In this study; different selected three isolates of Aspergillus flavus (No 1, 2 and 3) were screened for their ability to produced kojic acid and the isolate No 3 was the highest kojic acid producer one. The capability of A. flavus No 3 to produce kojic acid was improved using Plackett-Burman design. From ten different agro-industrial wastes cane molasses recorded the highest kojic acid productivity with 2.24 g/l-1 day-1 and was the most effective parameter plays a crucial role in Plackett-Burman design. Maximum kojic acid production (24.65 g/l) by A. flavus (No. 3) obtained under the fermentation conditions: incubation temperature at 25oC, incubation time 9 days, pH 3, inoculum size 0.5%, shaking rate at 150 rpm and medium constituents: Cane molasses 60 g/l, yeast extract 7 g/l, KH2PO4 2 g/l, ZnSO4·7H2O 100 µg/l and MgSO4·7H2O 1 g/l with regression analysis (R2) 99.45% and 2.33-fold increase in comparison to the production of the original level (10.6 g/l). \u0000DOI: http://dx.doi.org/10.5281/zenodo.1211517","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77486990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Pesticides and food safety in Africa 非洲的农药和食品安全

European Journal of Biological Research Pub Date : 2018-03-22 DOI: 10.5281/ZENODO.1237542

A. Adewunmi, S. Fapohunda

引用次数: 8

Antioxidant potential of the farmer preferred selections of Solanum aethiopicum vegetable consumed in central Uganda 在乌干达中部消费的农民首选的埃塞俄比亚茄蔬菜的抗氧化潜力

European Journal of Biological Research Pub Date : 2018-03-10 DOI: 10.5281/ZENODO.1195552

S. Sekulya, A. Nandutu, A. Namutebi, J. Ssozi, M. Masanza, B. Kabod, J. Jagwe, A. Kasharu, D. Rees, E. Kizito

引用次数: 1

The relevance of sebum composition in the etiopathogeny of acne 皮脂成分在痤疮发病机制中的相关性

European Journal of Biological Research Pub Date : 2018-02-25 DOI: 10.5281/ZENODO.1184139

M. Cunha, F. Daza, C. M. Filho, G. Veiga, F. A. Fonseca

{"title":"The relevance of sebum composition in the etiopathogeny of acne","authors":"M. Cunha, F. Daza, C. M. Filho, G. Veiga, F. A. Fonseca","doi":"10.5281/ZENODO.1184139","DOIUrl":"https://doi.org/10.5281/ZENODO.1184139","url":null,"abstract":"Acne vulgaris is an inflammatory disease that develops around the hair follicle. Many are the interconnected etiopathogenic factors involved, among which we can mention the increase in levels of androgen hormones, sebum hypersecretion, follicular hyperkeratosis with microcomedo formation, the proliferation of the bacteria Propionibacterium acnes ( P . acnes ) and the resulting inflammatory response. The way this bacterial growth occurs and how it is connected with the development of the inflammatory process have been themes of many clinical and experimental trials. Modifications in the sebum composition lead to a greater proliferation and differentiation of keratinocytes that obstruct the follicular ostium and favor the formation of comedones. On the other hand, these modifications alter the follicular hydration and facilitate the proliferation of the P . acnes , which not only produces chemotactic factors but also releases lipase that oxidizes the squalene. The oxidized squalene induces the formation of pro-inflammatory cytokines and boosts the innate immunity of keratinocytes and sebocytes, thus generating the inflammatory process. The aim of this study was to review the literature regarding the new concepts on the pathogenesis of acne.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77318083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of selenium on nutritive value of purslane (Portulaca oleracea L.) 硒对马齿苋营养价值的影响

European Journal of Biological Research Pub Date : 2018-01-01 DOI: 10.5281/ZENODO.1283418

K. Gamal, H. Salama, S. Ismaiel

{"title":"Effect of selenium on nutritive value of purslane (Portulaca oleracea L.)","authors":"K. Gamal, H. Salama, S. Ismaiel","doi":"10.5281/ZENODO.1283418","DOIUrl":"https://doi.org/10.5281/ZENODO.1283418","url":null,"abstract":"Purslane ( Portulaca oleracea ) one of the auxiliary plants was traditionally consumed in many parts of the world for its nutritional and medicinal benefits. The nutrient components of purslane such as total protein, total carbohydrates and mineral content such as macro elements (Na, K, Ca and Mg) and micro elements (Fe, Cu, Pb and Zn) were estimated at different concentrations of selenium which treated in soil where the plant cultivated. The protein and carbohydrate contents of leaves as well as protein of stems increase with increasing the selenium concentration, while protein and carbohydrate of roots as well as carbohydrate of stems decrease with increasing Se concentration. The mineral content was also affected by Se concentration, Fe, Cu and Zn of leaves decreased with increasing Se concentration, while K, Ca, Mg and Na are directly proportional with Se concentration. In stems, Zn only is inversely proportional with Se concentration. In roots, Fe, Cu, Mg and K are inversely proportional with Se concentration, while Na, Ca and Zn are directly proportional. The findings of this study revealed that carbohydrates, protein and mineral contents of purslane can be affected and controlled by selenium concentration.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85199854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthelminthic/larvicidal activity of some common medicinal plants 一些常见药用植物的杀虫活性

European Journal of Biological Research Pub Date : 2017-10-25 DOI: 10.5281/ZENODO.1036819

K. Sunita, Pradeep Kumar, M. Khan, Sadaf, S. Husain, D. Singh

{"title":"Anthelminthic/larvicidal activity of some common medicinal plants","authors":"K. Sunita, Pradeep Kumar, M. Khan, Sadaf, S. Husain, D. Singh","doi":"10.5281/ZENODO.1036819","DOIUrl":"https://doi.org/10.5281/ZENODO.1036819","url":null,"abstract":"The helminthic infection are most common disease in different animals and in human beings, which affecting a large proportion of the world population. Helminthic infection can also affect millions of livestock resulting in considerable economic loss in domestic animals. For control of helminthic disease in different part of world are uses synthetic medicines which are very effective in curing helminthiasis, but it’s also causes a number of side effects. The continued uses of synthetic anthelmintic/larvicidal drugs are also causing a major drug resistance problem in several parasitic diseases. The plant derived crude products are less efficient with respect to cure of parasitic diseases but one relatively free from side effect. A large number of medicinal plants are traditionally uses to cure helminthiasis in developing countries. Thus, plant derived drugs are gaining a lot of attention for curing parasitic infection. There are several medicinal plants and their different crude products, organic extracts and active components have been scrutinized for using in various methods in helminthic/larvicidal infection control. The present reviews summarized the use of traditional medicinal plants and their different products further leads to evaluation of new researches.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82024088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Statistical optimization as a powerful tool for indole acetic acid production by Fusarium oxysporum 统计优化是尖孢镰刀菌生产吲哚乙酸的有力工具

European Journal of Biological Research Pub Date : 2017-10-20 DOI: 10.5281/ZENODO.1012348

G. Mahmoud, H. Mostafa

{"title":"Statistical optimization as a powerful tool for indole acetic acid production by Fusarium oxysporum","authors":"G. Mahmoud, H. Mostafa","doi":"10.5281/ZENODO.1012348","DOIUrl":"https://doi.org/10.5281/ZENODO.1012348","url":null,"abstract":"Crop production is challenged in our world by increasing food demands, decrease natural resource bases and climatic change. Nowadays plant growth regulators works like fertilizers in increasing plant growth production efficiency and needed to produce in large industrial scale. Fermentation condition and medium constituents can significantly affect on the product production and designing an acceptable fermentation medium is critical importance. In this paper Fusarium sp. could be considered as promising indole-3-acetic acid producers with the ability to improve the production using statistical methods. The results showed that fermentation type, incubation temperature and L-tryptophan were the most influencing parameters on the production. Maximum IAA production by Fusarium oxysporum was 300.4 mg/l obtained under the fermentation conditions: temperature at 25 o C, incubation period 5 days, pH 7, inoculums size 2%, shaking rate at 150 rpm and medium constituents: Glucose 40 g/l, yeast extract 3 g/l, L-tryptophan 1 g/l, KH 2 PO 4 2 g/l, NaNO 3 4 g/l, MgSO 4 ·7H 2 O 0.1 g/l with regression analysis (R 2 ) 99.67% and 2.12-fold increase in comparison to the production of the original level (142 mg/l).","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86623402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Scorpion venom: pharmacological analysis and its applications 蝎毒:药理分析及其应用

European Journal of Biological Research Pub Date : 2017-09-27 DOI: 10.5281/ZENODO.998076

M. Chaubey

{"title":"Scorpion venom: pharmacological analysis and its applications","authors":"M. Chaubey","doi":"10.5281/ZENODO.998076","DOIUrl":"https://doi.org/10.5281/ZENODO.998076","url":null,"abstract":"Scorpions belong to class: Arachnida, order: Scorpionida represented now by approximately 1500 species. These are one of the most ancient group of the animals on the earth conserving their morphology almost unaltered and are the most successful inhabitants of the earth. Scorpions when stimulated secrete venom which is a cocktail of variable concentration of neurotoxins, cardiotoxins, nephrotoxins, hemolytic toxins, phosphodiesterases, phospholipases, hyaluronidase, glucosaminoglycans, histamine, seratonin, tryptophan and cytokine releasers. According to an estimate, frequency of deaths caused by scorpion sting is higher in comparison to that of caused by snake-bite. Almost all of these lethal scorpions except Hemiscorpious species belong to scorpion family Buthidae comprising 500 species. Scorpion venoms show variable reactions in envenomated patients. However, closer the phylogenic relationship among the scorpions, more similar the immunological properties. Furthermore, various constituents of venom may act directly or indirectly and individually or synergistically to exert their effects. Scorpion stings cause a wide range of conditions from severe local skin reactions to neurologic, respiratory and cardiovascular collapse. Lethal members of Buthidae family include Buthus , Parabuthus , Mesobuthus , Tityus , Leiurus , Androctonus and Centruroides . Besides their lethal properties, scorpion venoms have some unique properties beneficial to mankind. These contain anti-insect, antimicrobial and anticancer properties and thus, can play a key role in the insect pest management programmes, treatment of microbial infection and in the treatment of various cancer types.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83067844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In silico sequence analysis of predicted beta-amylase 7-like protein in Juglans regia L. 核桃β -淀粉酶7样蛋白预测序列分析。

European Journal of Biological Research Pub Date : 2017-05-24 DOI: 10.5281/ZENODO.583137

E. Sevindik

{"title":"In silico sequence analysis of predicted beta-amylase 7-like protein in Juglans regia L.","authors":"E. Sevindik","doi":"10.5281/ZENODO.583137","DOIUrl":"https://doi.org/10.5281/ZENODO.583137","url":null,"abstract":"Walnut ( Juglans regia L.) is a deciduous tree of the Juglandaceae family. Beta-amylase (β-amylase, EC 3.2.1.2) is an enzyme that catalyses hydrolysis of glycosidic bonds in polysaccharides. In this study; sequence, physicochemical, and three-dimensional analyses of predicted β-amylase 7-like protein in Juglans regia using various bioinformatic tools were conducted. The physicochemical properties of the predict β-amylase 7-like protein were analyzed by using ExPASy ProtParam tool that revealed the molecular weight (MW), Isoelectric Points ( pI ), total number of negatively charged residues (Asp + Glu), total number of positively charged residues (Arg + Lys), instability index, aliphatic index, and GRAVY (Grand Average of Hydropathy) values. Subcellular localization using CELLO v.2.5, putative phosphorylation sites using NetPhos 3.1 server, domain analysis using Pfam, and secondary structure prediction using SOPMA were accom-plished. To predict the 3D structure of the predict β-amylase 7-like protein, homology models were applied using PSIPRED, RAMPAGE, and PyMOL programs. The results of our study provide insight into fundamental characteristics of the predicted β-amylase 7-like protein in Juglans regia.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83595971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1