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Synergy of GH67 and GH115 α-1,2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-03-11 DOI: 10.1016/j.enzmictec.2025.110629
Xinxin Li , Lanyu Li , Alessia Manassero , Astrid Müller , Sumitha K. Reddy , Mirjam A. Kabel , Ronald P. de Vries , Peicheng Sun
{"title":"Synergy of GH67 and GH115 α-1,2-glucuronidases with Penicillium subrubescens endoxylanases to stimulate xylooligosaccharide production","authors":"Xinxin Li ,&nbsp;Lanyu Li ,&nbsp;Alessia Manassero ,&nbsp;Astrid Müller ,&nbsp;Sumitha K. Reddy ,&nbsp;Mirjam A. Kabel ,&nbsp;Ronald P. de Vries ,&nbsp;Peicheng Sun","doi":"10.1016/j.enzmictec.2025.110629","DOIUrl":"10.1016/j.enzmictec.2025.110629","url":null,"abstract":"<div><div>A primary substitution of the plant cell wall hemicellulosic polysaccharide xylan is (4-<em>O</em>-methyl-)<span>d</span>-glucuronic acid, which hinders the endoxylanases (XLNs) degradation of xylan for the production of valuable xylooligosaccharides (XOS). In this context, α-1,2-glucuronidase (AGU) plays a critical role in hydrolyzing the α-(1→2)-glycosidic linkages between 4-<em>O</em>-methyl-<span>d</span>-glucuronic acid and xylosyl residues in xylan, thereby enhancing XOS production by XLNs. However, AGUs have been relatively poorly studied, and insufficient and incomplete data on their biochemical properties, substrate specificity, and product profiling has limited their application. Here, we cloned, heterologously produced, purified and functionally characterized an AGU from <em>Aspergillus niger</em> (<em>An</em>AguA) and another AGU from <em>Penicillium subrubescens</em> (<em>Ps</em>AguB), belonging to Glycoside Hydrolase family 67 (GH67) and 115 (GH115), respectively, in the Carbohydrate-Active enZyme database. Results showed that neither AGU released 4-<em>O</em>-methyl-<span>d</span>-glucuronic acid from polymeric beech wood glucuronoxylan (BeWX). However, we found that from BeWX pre-digested with GH10 or GH11 XLNs from <em>P. subrubescens</em> (<em>Ps</em>XlnA and <em>Ps</em>XlnF, respectively), <em>An</em>AguA released 4-<em>O</em>-methyl-<span>d</span>-glucuronic acid only from the non-reducing end of glucuronoxylan oligosaccharide, whereas <em>Ps</em>AguB released 4-<em>O</em>-methyl-<span>d</span>-glucuronic acid from glucuronoxylan oligosaccharides regardless of the xylosyl substitution position. Furthermore, we demonstrated that enhancement of XOS release by adding AGUs to various combinations of GH10 (<em>Ps</em>XlnA–C) and GH11 (<em>Ps</em>XlnD–F, <em>Ps</em>XlnH–I) XLNs from <em>P. subrubescens</em> varied based on the AGU-XLN combination. The combination of <em>An</em>AguA with <em>Ps</em>XlnA was the most effective, achieving at least a 3-fold increase in the release of XOS with a degree of polymerization of 5–7 compared to using <em>Ps</em>XlnA alone.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"187 ","pages":"Article 110629"},"PeriodicalIF":3.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acetic acid production from corn straw via enzymatic degradation using putative acetyl esterase from the metagenome assembled genome
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-03-04 DOI: 10.1016/j.enzmictec.2025.110619
Hao Chen , Zhiwei Zeng , Rup Lal , Jie Wu , Jia Chen , Mei Li , Lulu Cao , Xiqiang Liu , Ruzhe Zhang , Chunjie Gong
{"title":"Acetic acid production from corn straw via enzymatic degradation using putative acetyl esterase from the metagenome assembled genome","authors":"Hao Chen ,&nbsp;Zhiwei Zeng ,&nbsp;Rup Lal ,&nbsp;Jie Wu ,&nbsp;Jia Chen ,&nbsp;Mei Li ,&nbsp;Lulu Cao ,&nbsp;Xiqiang Liu ,&nbsp;Ruzhe Zhang ,&nbsp;Chunjie Gong","doi":"10.1016/j.enzmictec.2025.110619","DOIUrl":"10.1016/j.enzmictec.2025.110619","url":null,"abstract":"<div><div>Acetic acid production from corn straw by enzyme catalysis shows its application value in food industry. In this study, a gene encoding for a putative acetyl esterase derived from <em>Sphingobacterium soilsilvae</em> Em02 was discovered in metagenome assembled genome. The gene was expressed in <em>Escherichia coli</em> BL21 to obtain enzyme with a molecular mass of 38.8 kDa. P-Nitrophenyl acetate was used as a substrate to determine the enzyme activity. The enzyme demonstrated optimal activity under conditions of 40 °C and a neutral pH of 7.0. Under optimal conditions, 17.58 mg of acetic acid was obtained using the enzyme from 50 mg corn straw pretreated with amylase. The acetyl esterase derived from <em>Sphingobacterium soilsilvae</em> Em02, demonstrates significant potential for biotechnological applications, particularly in biomass degradation.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"187 ","pages":"Article 110619"},"PeriodicalIF":3.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recombinant alginate lyases and mannitol dehydrogenase enhance hydrolysis of macroalgal carbohydrates
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-26 DOI: 10.1016/j.enzmictec.2025.110618
Dominique F. Rocher, Marthinus W. Myburgh, Marinda Viljoen-Bloom, Rosemary A. Cripwell
{"title":"Recombinant alginate lyases and mannitol dehydrogenase enhance hydrolysis of macroalgal carbohydrates","authors":"Dominique F. Rocher,&nbsp;Marthinus W. Myburgh,&nbsp;Marinda Viljoen-Bloom,&nbsp;Rosemary A. Cripwell","doi":"10.1016/j.enzmictec.2025.110618","DOIUrl":"10.1016/j.enzmictec.2025.110618","url":null,"abstract":"<div><div>Brown macroalgae are a promising source for bioethanol production, primarily due to their high carbohydrate, low lignin and high moisture content. Bioconversion of macroalgae to ethanol requires a yeast, such as <em>Saccharomyces cerevisiae</em>, that can hydrolyse the macroalgal carbohydrates, namely laminarin, mannitol and alginate. In this study, the mannitol dehydrogenase (MDH) genes from <em>Aspergillus fumigatus</em> (<em>AfMDH</em>) and <em>Talaromyces islandicus</em> (<em>TiMDH</em>), and the alginate lyase (AL) genes from <em>Sphingomonas</em> sp. (<em>SpxAL</em> and <em>SpeAL</em>) and <em>Talaromyces emersonii</em> (<em>TeeAL</em>) were expressed in the laboratory strain, <em>S. cerevisiae</em> Y294. Co-cultures of a laminarinase-producing yeast, Y294[Relam1/Tvlam1] and yeasts expressing mannitol dehydrogenases and alginate lyases were evaluated for the consolidated bioprocessing of the major carbohydrates in brown macroalgae. Laminarin and mannitol were targeted for ethanol production, while alginate was depolymerised to expose mannitol. A co-culture of <em>S. cerevisiae</em> Y294[Relam1/Tvlam1], [AfMDH] and [TeeAL/SpxAL] strains produced 10.30 g/L ethanol from <em>Ecklonia maxima</em>, representing a 98 % carbon conversion (based on the laminarin and mannitol content). A strain expressing both endo- and exo-alginate lyase improved the ethanol yield by 42.28 % compared to strains expressing only laminarinase- and mannitol dehydrogenase. Scanning electron microscopy further revealed that co-cultures containing laminarinase, MDH, and AL enzymes promoted significant physical degradation and increased porosity in macroalgal substrates, suggesting enhanced alginate hydrolysis and improved enzyme accessibility. This is the first report on the simultaneous hydrolysis of mannitol, alginate and laminarin with recombinant enzymes during macroalgal fermentation. The results demonstrate significant progress towards exploiting brown macroalgae for bioconversion to ethanol and high-value products.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"186 ","pages":"Article 110618"},"PeriodicalIF":3.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergy between processive cellulases in Ruminoccocus albus
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-18 DOI: 10.1016/j.enzmictec.2025.110610
Alem Storani , Alberto A. Iglesias, Sergio A. Guerrero
{"title":"Synergy between processive cellulases in Ruminoccocus albus","authors":"Alem Storani ,&nbsp;Alberto A. Iglesias,&nbsp;Sergio A. Guerrero","doi":"10.1016/j.enzmictec.2025.110610","DOIUrl":"10.1016/j.enzmictec.2025.110610","url":null,"abstract":"<div><div>Endoglucanases (EGs), cellobiohydrolases (CBHs), and β-glucosidases are essential components in enzymatic degradation of cellulose. We analyzed the glycosyl hydrolases from families GH5 and GH48 from <em>Ruminococcus albus</em> 8 (<em>Ral</em>Cel5G and <em>Ral</em>Cel48A). Both enzymes feature a catalytic motif and a carbohydrate binding domain from family 37 (CBM37). <em>Ral</em>Cel5G also exhibited a second CBM37 with lower similarity. As a result, <em>Ral</em>Cel5G showed higher binding affinity toward insoluble substrates and broader recognition capacity. Kinetic characterization using different cellulosic substrates and reaction product analysis confirmed <em>Ral</em>Cel5G as a processive EG while <em>Ral</em>Cel48A is a CBH. Interestingly, we found a synergistic effect on their activity at a low EG to CBH ratio, despite the processive activity of <em>Ral</em>Cel5G. Furthermore, the lignocellulose degradation capacity was improved by supplementing the cellulases with hemicellulase <em>Ral</em>Xyn10A. These results provide valuable information about the interaction between processive EG and conventional CBH, necessary for the rational design of enzyme cocktails for optimized biomass processing.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"186 ","pages":"Article 110610"},"PeriodicalIF":3.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of keratinase from Chryseobacterium camelliae Dolsongi-HT1 and efficacy on skin exfoliation
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-17 DOI: 10.1016/j.enzmictec.2025.110605
Eun-Mi Kim, Soojung Oh, Hyeongwon Choi, Won-Seok Park
{"title":"Characterization of keratinase from Chryseobacterium camelliae Dolsongi-HT1 and efficacy on skin exfoliation","authors":"Eun-Mi Kim,&nbsp;Soojung Oh,&nbsp;Hyeongwon Choi,&nbsp;Won-Seok Park","doi":"10.1016/j.enzmictec.2025.110605","DOIUrl":"10.1016/j.enzmictec.2025.110605","url":null,"abstract":"<div><div>Keratin is the outermost layer that protects our skin and has an appropriate turnover cycle. With age, the keratin turnover cycle begins to dysfunction. To overcome this issue, we artificially remove dead skin cells. In this study, we attempted to screen enzymes that could be useful in the cosmetics industry to develop enzymes suitable for the enzyme-based method, a mild exfoliation method that does not damage the skin. <em>Chryseobacterium camelliae</em> Dolsongi-HT1 with keratinolytic activity was isolated from green tea leaves (sourced from the Dolsongi tea garden, Jeju Island). The keratinolytic activity of <em>C. camelliae</em> Dolsongi-HT1 was detected in the culture media, indicating that the target keratinolytic enzyme is a secreted protein. Keratinolytic activity was demonstrated using forearm skin keratin and reconstituted human skin models. The enzyme from <em>C. camelliae</em> Dolsng-HT1 (HT1) could efficiently decompose human skin keratin. Moreover, experiments using the reconstituted human skin model demonstrated that HT1 is efficient in exfoliating the outermost stratum corneum. Compared with the popularly used chemical exfoliation method, enzymatic exfoliation using HT1 was less abrasive and did not damage the epidermal layer. Keratinolytic enzyme was identified using protein purification and mass spectrometry. The identified enzyme (iHT1) was expressed in the <em>Bacillus subtilis</em> RIK 1285 secretory protein expression system. The iHT1 enzyme showed high activity over a wide temperature range (30–60 °C), with the highest activity at 30 °C. The optimum pH for the activity of iHT was pH8.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"186 ","pages":"Article 110605"},"PeriodicalIF":3.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation 高聚合度壳寡糖的酶法生产及其在酱油保鲜中的潜在应用
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-17 DOI: 10.1016/j.enzmictec.2025.110608
Yihao Liu , Guangru Sun , Yimeng Lou , Peng Cheng , Qian Song , Wen Lv , Chunling Wang
{"title":"Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation","authors":"Yihao Liu ,&nbsp;Guangru Sun ,&nbsp;Yimeng Lou ,&nbsp;Peng Cheng ,&nbsp;Qian Song ,&nbsp;Wen Lv ,&nbsp;Chunling Wang","doi":"10.1016/j.enzmictec.2025.110608","DOIUrl":"10.1016/j.enzmictec.2025.110608","url":null,"abstract":"<div><div>Chitooligosaccharides (COSs) with a high degree of polymerisation (DP 5–10) have been reported to possess diverse bioactivities. Thus, the development of novel methods for the acquisition of high-DP COSs has become increasingly significant. In the study, a novel GH family 46 chitosanase gene (<em>ThCsn46</em>) was expressed and characterized. ThCsn46 was further applied to COSs production, and the highest yield of 95.7 % (143.6 g/L) was obtained using 15 % (w/v) of chitosan as the substrate. The proportion of high-DP COSs occupied 40.6 % of the total COSs. Moreover, the high (GlcN)<sub>6</sub> content was achieved. The total viable count (TVC) and amino acid nitrogen (AAN) of soy sauce incorporated with 0.1 % (w/v) of COSs were better than that of the negative control. The potential of ThCsn46 for application in the production of COSs and the preservation of soy sauce is significant. The green and efficient bioproduction process represents a promising way for further research.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"186 ","pages":"Article 110608"},"PeriodicalIF":3.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening and characterization of thermostable xylose isomerase from Rhodothermus marinus for erythrose production from one-carbon source 筛选和鉴定海红藻中的可热稳定木糖异构酶,以利用单碳源生产赤藓糖
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-17 DOI: 10.1016/j.enzmictec.2025.110607
Yanfei Wang , Wenwen Li , Dandan Wang , Yan Zeng , Ming Li , Yuanxia Sun , Jiangang Yang
{"title":"Screening and characterization of thermostable xylose isomerase from Rhodothermus marinus for erythrose production from one-carbon source","authors":"Yanfei Wang ,&nbsp;Wenwen Li ,&nbsp;Dandan Wang ,&nbsp;Yan Zeng ,&nbsp;Ming Li ,&nbsp;Yuanxia Sun ,&nbsp;Jiangang Yang","doi":"10.1016/j.enzmictec.2025.110607","DOIUrl":"10.1016/j.enzmictec.2025.110607","url":null,"abstract":"<div><div>Four-carbon (C4) sugars, which rarely exist in nature, usually have various biological functions and serve as building units for pharmaceutical agents. For example, erythrose possesses various pharmacological activities. Although several iso/epimerases have a catalytic function on C5/C6 sugars, only a few studies have demonstrated the enzymatic iso/epimerization of C4 sugars. In this work, we presented a xylose isomerase from <em>Rhodothermus marinus</em> possessing isomerization activity toward C4 <span>l</span>-erythrulose. This enzyme showed higher affinity and catalytic efficiency toward <span>l</span>-erythrulose than <span>d</span>-fructose and <span>d</span>-xylulose. Its specific activity reached 24.2 U/mg under optimal reaction conditions, and its half-life was over 8 days at 50 ℃. Demonstration of this enzyme under 833 mM of L-erythrulose gave 130 mM L-erythrose with a conversion yield of 15.6 %. On the basis of this beneficial enzyme, we further designed a multienzyme system and presented a one-pot cascade process for the synthesis of <span>l</span>-erythrose from low-cost, one-carbon formaldehyde (FALD) as the sole feedstock. Given that FALD can be derived from CO<sub>2</sub> electrocatalysis or methanol oxidization, <span>l</span>-erythrose synthesis can be realized from methanol and even CO<sub>2</sub>. <span>l</span>-erythrose can further function as feedstock for synthesizing other high-carbon sugars by coupling enzymatic aldol condensation and reduction reactions.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"186 ","pages":"Article 110607"},"PeriodicalIF":3.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Processivity and enzymatic mechanism of a non-modular family 5 endoglucanase from Sporocytophaga sp. CX11 with potential applications in cellulose saccharification
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-15 DOI: 10.1016/j.enzmictec.2025.110609
Xiaoyi Chen , Zilong Gao , Shang Wang, Fan Yang
{"title":"Processivity and enzymatic mechanism of a non-modular family 5 endoglucanase from Sporocytophaga sp. CX11 with potential applications in cellulose saccharification","authors":"Xiaoyi Chen ,&nbsp;Zilong Gao ,&nbsp;Shang Wang,&nbsp;Fan Yang","doi":"10.1016/j.enzmictec.2025.110609","DOIUrl":"10.1016/j.enzmictec.2025.110609","url":null,"abstract":"<div><div>In this study, a novel GH5 processive endoglucanase <em>Sm</em>Cel5A from <em>Sporocytophaga</em> sp. CX11 was functionally expressed in <em>E. coli.</em> It could rapidly decrease the viscosity of carboxymethyl cellulose (CMC-Na) solution by nearly 50 % within the initial 8 min of incubation and exhibited a significantly high specific activity towards CMC-Na of 9940 U/µmol. <em>Sm</em>Cel5A could also hydrolyze other cellulosic substrates such as RAC, Avicel, filter paper, β-glucan and the chromogenic substrate <em>p</em>NPC. When hydrolyzing filter paper, about 89.1 % of soluble reducing sugars were generated after 180 min of incubation, and the main products were cellobiose followed by cellotriose and glucose. The processive ratio of <em>Sm</em>Cel5A increased from 2.32 to 11.22 as the reaction time was extended from 5 min to 180 min, which is significantly higher than those of other known processive endoglucanases. Moreover, <em>Sm</em>Cel5A could hydrolyze cellodextrins with the degree of polymerization (DP) ≥ 3, but it was not active on cellobiose. In combination reaction with β-glucosidase, the maximum substrate conversion rate reached 73.2 %, showing that the synergistic reaction of the two enzymes could efficiently reduce the accumulation of cellobiose and greatly improve the hydrolysis efficiency of cellulose. The three-dimensional structure of <em>Sm</em>Cel5A was predicted by AlphaFold2 and showed to feature a classic GH5 family (β/α)<sub>8</sub>-barrel structure, with a deep substrate-binding cleft formed by the amino acids at the C-terminus. Molecular docking results indicated that when hydrolyzing cellulosic substrates, <em>Sm</em>Cel5A exhibits a preference for the exo-type mechanism of action over the endo-type mechanism of action.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"185 ","pages":"Article 110609"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering the D-lactic acid responsive promoter/repressor system as dynamic metabolic engineering tool in Lactobacillus delbrueckii subsp. bulgaricus for controlled D-lactic acid biosynthesis
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-07 DOI: 10.1016/j.enzmictec.2025.110606
Payal Mukherjee , Senthilkumar Sivaprakasam
{"title":"Engineering the D-lactic acid responsive promoter/repressor system as dynamic metabolic engineering tool in Lactobacillus delbrueckii subsp. bulgaricus for controlled D-lactic acid biosynthesis","authors":"Payal Mukherjee ,&nbsp;Senthilkumar Sivaprakasam","doi":"10.1016/j.enzmictec.2025.110606","DOIUrl":"10.1016/j.enzmictec.2025.110606","url":null,"abstract":"<div><div>Dynamic metabolic engineering integrates synthetic logic circuits into cellular systems, optimizing metabolic fluxes and augmenting biosynthesis of target metabolites. This study evaluated a D-lactic acid (DLA)-responsive promoter-repressor system from <em>Pseudomonas fluorescens</em> A506, re-engineered for heightened sensitivity and functional efficacy in <em>Lactobacillus delbrueckii</em> subsp. <em>bulgaricus</em> VI104. The codon-optimized regulatory architecture exhibited peak performance at DLA inducer concentration range of 60–100 mM, validated by fluorometry and microscopy. As an application, overexpression of D-lactate dehydrogenase (<em>dldh</em>) downstream of the engineered promoter repressor system enabled finely tuned modulation of DLA biosynthesis, autonomously regulating the transition between growth and production phases, thereby attenuating overall metabolic load. Cross-species compatibility was confirmed by excising regulatory elements from the promoter-repressor system and functionally assessing them in recombinant <em>L. bulgaricus</em>. Molecular docking elucidated critical noncovalent interactions between D-<em>LldR</em> repressor and operator nucleotide sequence in absence of inducer DLA. The engineered promoter construct with high efficiency effectively elevated DLA biosynthesis by 2.15-folds and expanded the overall fermentation time relative to constitutive systems, attaining maximum DLA titre of 9.02 g L⁻<sup>1</sup> in bioreactor setup. These results substantially broaden the molecular cloning toolkit available for <em>L. bulgaricus</em>, fostering potential future applications in biotherapeutics and probiotics.</div></div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"185 ","pages":"Article 110606"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of 2’-deoxyguanosine for an α-amylase inhibitor in the extracts of the earthworm Eisenia fetida and characterization of its inhibitory activity against porcine pancreatic α-amylase
IF 3.4 3区 生物学
Enzyme and Microbial Technology Pub Date : 2025-02-04 DOI: 10.1016/j.enzmictec.2025.110604
Masako Ogasawara , Katsuhiro Yoshii , Jun Wada , Yoshihiro Yamamoto , Kuniyo Inouye
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