Environmental Toxicology最新文献

{"title":"Chlorpyrifos Induces Apoptosis in Macrophages by Activating Both Intrinsic and Extrinsic Apoptotic Pathways.","authors":"Chen-Yu Chiang, Shin-Wu Liu, Chun-Jung Chen, Wen-Ying Chen","doi":"10.1002/tox.24515","DOIUrl":"https://doi.org/10.1002/tox.24515","url":null,"abstract":"<p><p>Although chlorpyrifos poses considerable risks to the environment and human health, it is still used in many countries. This pesticide has various toxic effects on humans, including neurotoxicity, reproductive toxicity, genotoxicity, and organ damage caused by oxidative stress and DNA damage. However, its specific toxicity to the immune system remains unclear. In this study, we explored the intrinsic and extrinsic apoptotic pathways through which chlorpyrifos induces apoptosis in macrophages. RAW 264.7 macrophages were treated with chlorpyrifos at concentrations of 0, 2, 4, 10, and 20 ppm for 3 h. Cytotoxicity was assessed using a lactate dehydrogenase assay, whereas apoptosis was evaluated through flow cytometry. The levels of cysteinyl aspartate-specific proteinase (caspase)-3, caspase-8, and caspase-9 were measured. The disruption of mitochondrial function and the expression of the death receptors Fas receptor and tumor necrosis factor-alpha receptor were assessed through JC-1 stain reagent. The release of mitochondrial cytochrome c, expression of Bcl2 family proteins, and level of cleaved caspases were analyzed through Western blotting. Chlorpyrifos induced cytotoxicity and apoptosis in a concentration-dependent manner. It activated caspase-3, caspase-8, and caspase-9, as well as disrupted mitochondrial function and Bcl2 family protein balance. Furthermore, chlorpyrifos induced the release of cytochrome c from the mitochondria and upregulated the expression of Fas receptor and tumor necrosis factor-alpha receptor. These findings suggest that chlorpyrifos induces cytotoxicity through caspase-3-dependent apoptosis via the intrinsic pathway (caspase-8 activation, mitochondrial dysfunction, Bcl2 protein imbalance, and cytochrome c release) and the extrinsic pathway (caspase-9 activation and death receptor expression).</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"METTL3-Mediated m6A Modification of Pri-miR-148a-3p Affects Prostate Cancer Progression by Regulating TXNIP\".","authors":"","doi":"10.1002/tox.24513","DOIUrl":"https://doi.org/10.1002/tox.24513","url":null,"abstract":"","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Choline Alleviated Perinatal Fluoride Exposure-Induced Learning and Memory Impairment Through α4β2 nAChRs and α7nAChRs in Offspring Mice\".","authors":"","doi":"10.1002/tox.24488","DOIUrl":"https://doi.org/10.1002/tox.24488","url":null,"abstract":"","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Causal Relationship Between 731 Immunocyte Phenotypes and Asthma Risk: A Bidirectional Mendelian Randomization Analysis","authors":"Jie Lin, Zhan Zhang, Qiang Zhou, Jiayi Shi, Wanqing Li, Sen Lin, Hu Tang, Zheyu Zhang, Tianfeng Chen, Xiaojun Cai, Longhe Cao","doi":"10.1002/tox.24506","DOIUrl":"https://doi.org/10.1002/tox.24506","url":null,"abstract":"Growing evidence suggests an association between various immune cell phenotypes and the development of asthma. However, it is still not entirely clear whether specific immune cell phenotypes might causally contribute to the risk of asthma. Despite further studies required to validate this claim, our study delves deeper into explaining this relationship and paving the way toward new therapeutic approaches. Our initial aim is to reveal the causal relationship between 731 immunocyte phenotypes and asthma; a bidirectional Mendelian randomization (MR) analysis was performed. We have investigated 731 immunocyte phenotypes in asthma, using a summary of previously published GWAS. Conducting an MR analysis, we have interpreted the potential causative relationship between them. Our analytical approaches included inverse variance‐weighted average, weighted median (WM) modeling, and pattern‐based approaches. To ensure the robustness and accuracy of our findings, we conducted sensitivity analyses employing MR‐PRESSO, Cochran's <jats:italic>Q</jats:italic> test, leave‐one‐out, and MR‐Egger approaches. Additionally, we conducted a reverse MR analysis to examine potential reverse causality. Our study revealed 43 immunophenotypes with a causal connection to asthma risk. Following Bonferroni correction and sensitivity analysis, we have identified four immunophenotypes with strong causal associations and reliability, them being: HLA DR on DC (95% CI: 1.0008–1.0014, <jats:italic>p</jats:italic> = 4.26 × 10<jats:sup>−6</jats:sup>, FDR = 2.21 × 10<jats:sup>−8</jats:sup>), CD8 on CD28− CD8br (95% CI: 1.0007–1.0020, <jats:italic>p</jats:italic> = 4.01 × 10<jats:sup>−5</jats:sup>，FDR = 5.70 × 10<jats:sup>−4</jats:sup>), CD62L on monocyte (95% CI: 0.9985–0.9995，<jats:italic>p</jats:italic> = 1.06 × 10<jats:sup>−4</jats:sup>，FDR = 9.20 × 10<jats:sup>−4</jats:sup>), CD8br% leukocyte (95% CI: 1.0006–1.0019，<jats:italic>p</jats:italic> = 1.07 × 10<jats:sup>−4</jats:sup>，FDR = 1.14 × 10<jats:sup>−3</jats:sup>). We have confirmed the large co‐inheritance of all these immunophenotypes, which suggests a contribution to asthma development. In addition, reverse MR confirmed that asthma and immune cell phenotypes lack a causal association between them. Our study provides evidence of different immune phenotypes, which are either beneficial or detrimental to asthma. Given the fact that asthma is a broad disease and contains complex immune mechanisms, this research may offer improved outcomes and long‐term asthma treatment strategies.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"4 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Transcriptional Factor BRD4 Promotes the Stemness of Esophageal Cancer by Activating the Nuclear PD-L1/RelB Axis.","authors":"","doi":"10.1002/tox.24508","DOIUrl":"https://doi.org/10.1002/tox.24508","url":null,"abstract":"<p><strong>Retraction: </strong>S. Li, Q. Guo, R. Guo, and H. Xu, \"Transcriptional Factor BRD4 Promotes the Stemness of Esophageal Cancer by Activating the Nuclear PD-L1/RelB Axis,\" Environmental Toxicology 39, no. 2 (2024): 669-679, https://doi.org/10.1002/tox.23939. The above article, published online on 24 August 2023, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Paul Tchounwou; and Wiley Periodicals LLC. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Pathogenomics Model for Personalized Medicine in Cervical Cancer: Cross-Talk of Gene Expressions and Pathological Images Related to Oxidative Stress.","authors":"","doi":"10.1002/tox.24510","DOIUrl":"https://doi.org/10.1002/tox.24510","url":null,"abstract":"<p><strong>Retraction: </strong>J. Li, S. Chen, J. Wu, X. Liu, H. Liu, Y. Liu, and Z. Zhu, \"Pathogenomics Model for Personalized Medicine in Cervical Cancer: Cross-Talk of Gene Expressions and Pathological Images Related to Oxidative Stress,\" Environmental Toxicology 39, no. 2 (2024): 751-767, https://doi.org/10.1002/tox.23974. The above article, published online on 26 September 2023, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Paul Tchounwou; and Wiley Periodicals LLC. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Comprehensive Analysis of m6A Modification Patterns and m6A-Related lncRNAs as Potential Biomarkers in Lung Adenocarcinoma.","authors":"","doi":"10.1002/tox.24512","DOIUrl":"https://doi.org/10.1002/tox.24512","url":null,"abstract":"<p><strong>Retraction: </strong>S. Wang, X. Gu, D. Xu, B. Liu, K. Qin, and X. Yuan, \"Comprehensive Analysis of m6A Modification Patterns and m6A-Related lncRNAs as Potential Biomarkers in Lung Adenocarcinoma,\" Environmental Toxicology 39, no. 4 (2024): 2285-2303, https://doi.org/10.1002/tox.24110. The above article, published online on 26 December 2023, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Christyn Bailey; and Wiley Periodicals LLC. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted. Sheng Wang disagrees with this decision. Xuyu Gu was unaware that they were included as an author on this submission and was not involved with its creation.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: SIK2-Positive Tumor Epithelial Cells in Breast Cancer Maybe Potential Anti-Cancer Messengers: A Systematic Analysis from a Single-Cell Perspective.","authors":"","doi":"10.1002/tox.24511","DOIUrl":"https://doi.org/10.1002/tox.24511","url":null,"abstract":"<p><strong>Retraction: </strong>Q. Wu, C. Qu, L. Xin, F. Yang, and L. Xu, \"SIK2-Positive Tumor Epithelial Cells in Breast Cancer Maybe Potential Anti-Cancer Messengers: A Systematic Analysis from a Single-Cell Perspective,\" Environmental Toxicology 39, no. 2 (2024): 768-782, https://doi.org/10.1002/tox.23987. The above article, published online on 29 September 2023, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Paul Tchounwou; and Wiley Periodicals LLC. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: An Innovative Predictive Model for Cervical Cancer Constructed Around a Gene Profile Associated With Cholesterol Metabolism.","authors":"","doi":"10.1002/tox.24509","DOIUrl":"https://doi.org/10.1002/tox.24509","url":null,"abstract":"<p><strong>Retraction: </strong>T. Pu, Y. Jin, C. Tang, J. Fu, C. Zhang, B. Su, and A. Cao, \"An Innovative Predictive Model for Cervical Cancer Constructed Around a Gene Profile Associated with Cholesterol Metabolism,\" Environmental Toxicology 39, no. 3 (2024): 1055-1071, https://doi.org/10.1002/tox.23969. The above article, published online on 11 September 2023, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Paul Tchounwou; and Wiley Periodicals LLC. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct Disruptive Effects of Methylparaben and Propylparaben on the Prostate and Gonads of Adult Gerbils.","authors":"Edvaldo Mendes Silva, Janaína Ribeiro Costa, Ariany Angéllica Caballero Corrêa, Naiara Cristina de Souza Ribeiro, Hericles Mesquita Campos, Elizabeth Pereira Mendes, Mara Rubia Marques, Sebastião Roberto Taboga, Paulo César Ghedini, Sérgio Alexandre Alcantara Dos Santos, Manoel Francisco Biancardi, Fernanda Cristina Alcantara Dos Santos","doi":"10.1002/tox.24507","DOIUrl":"https://doi.org/10.1002/tox.24507","url":null,"abstract":"<p><p>Parabens are chemicals used as preservatives in food, personal care products, and pharmaceuticals. Detectable paraben levels are found in water, air, and soil, making human and animal exposure unavoidable. This study evaluated the effects of methylparaben, propylparaben, and their mixture on the prostate and gonads of male and female gerbils. Gerbils aged 90 days were assigned to the groups: (M) 10 mg/kg/day of methylparaben; (P) 10 mg/kg/day of propylparaben; (M + P) 5 mg/kg/day of methylparaben plus 5 mg/kg/day of propylparaben; (C) control group. The animals were treated orally for 30 consecutive days, and the prostates and gonads were subjected to morphometric-stereological, immunohistochemical, and biochemical analyses. The male ventral prostates in the P and M + P groups showed an increase in relative weight, higher cell proliferation, besides the occurrence of hyperplasia and epithelial desquamation. All treatments reduced malondialdehyde levels in the male prostate. The female prostate exhibited alterations in all treated groups, but with greater severity in the P and M + P groups. These alterations included increased cell proliferation and epithelial hyperplasia, as well as stromal inflammatory foci and increased secretory activity. M, P, and M + P reduced the diameter of the seminiferous tubules, the thickness of the germinal epithelium, and the catalase activity in the testes. In the ovaries, P and M + P reduced the primary follicle number and increased the superoxide dismutase activity. These results indicate that propylparaben is more harmful to the prostate and gonads than methylparaben, and that the acceptable daily intake for these parabens is not safe for reproductive health.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}