Environmental Toxicology最新文献

Ecotoxicological Evaluation of Organic Sunscreens Used Worldwide, Alone and in Mixture, on Terrestrial Plants 世界范围内单独和混合使用的有机防晒霜对陆生植物的生态毒理学评价

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-10-07 DOI: 10.1002/tox.24571

Diego Espirito Santo, Edson Araújo de Almeida, Elisângela Dusman, A. C. Downs, Regiane da Silva Gonzalez, Osvaldo Valarini Junior, Ana Paula Peron

{"title":"Ecotoxicological Evaluation of Organic Sunscreens Used Worldwide, Alone and in Mixture, on Terrestrial Plants","authors":"Diego Espirito Santo, Edson Araújo de Almeida, Elisângela Dusman, A. C. Downs, Regiane da Silva Gonzalez, Osvaldo Valarini Junior, Ana Paula Peron","doi":"10.1002/tox.24571","DOIUrl":"https://doi.org/10.1002/tox.24571","url":null,"abstract":"The environmental hazards of sunscreens are discussed worldwide. However, there are few ecotoxicological studies on these compounds alone for edaphic organisms, and none for their mixtures. Avobenzone (1 and 10 ng/L), octocrylene (10 and 100 μg/L), and oxybenzone (2 and 20 μg/L), alone and in binary combinations (between the lowest and the highest concentrations), were evaluated for phytotoxicity to the roots of rustic varieties of Daucus carota and Solanum lycopersicum, and for cytotoxicity and genotoxicity in the roots of Allium cepa bulbs. In contrast to the higher concentrations, the lower concentrations of sunscreens, despite the increase in superoxide and hydroxyl radicals in the cells, did not cause changes in the length of the rootlets, since they did not affect cell elongation. In mixture, the lower concentrations caused a synergistic interaction in the roots, while the higher did not exceed the toxicity of the filters alone. In bulbs, the filters alone and in mixtures caused inhibition of cell division and mitotic spindle alterations in the meristems, mainly due to the accumulation of H2O2 in the cells, and the mixtures triggered a synergistic interaction in the roots. The mixtures were highly hazardous, especially avobenzone‐oxybenzone, the most absorbed by the roots and with the greatest phytotoxic and cytogenotoxic potential; however, of low environmental stability. Environmentally stable mixtures with octocrylene were the least absorbed but were highly harmful, inducing phytotoxicity and cytogenotoxicity in the plants. Therefore, the use of sewage sludge and wastewater on crop soils poses a risk to agricultural productivity and the environment.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"24 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Microbial Sensing via Resazurin Reduction Catalyzed by Graphene Oxide, A Versatile Approach for Diagnostics and Electrochemical Applications 通过氧化石墨烯催化reazurin还原增强微生物传感，一种用于诊断和电化学应用的通用方法

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-10-02 DOI: 10.1002/tox.24572

Valentina Palmieri, Marco de Spirito, Massimiliano Papi

{"title":"Enhanced Microbial Sensing via Resazurin Reduction Catalyzed by Graphene Oxide, A Versatile Approach for Diagnostics and Electrochemical Applications","authors":"Valentina Palmieri, Marco de Spirito, Massimiliano Papi","doi":"10.1002/tox.24572","DOIUrl":"https://doi.org/10.1002/tox.24572","url":null,"abstract":"Resazurin is a cell viability phenoxazine dye widely employed for bacterial monitoring, as its colorimetric and fluorometric conversion reflects microbial metabolic activity. In this work, we demonstrate that graphene oxide (GO), a two‐dimensional nanomaterial with high surface reactivity, markedly accelerates the reduction of resazurin in the presence of Staphylococcus aureus, enabling rapid microbial detection at non‐cytotoxic concentrations. Importantly, this GO‐mediated enhancement directly supports applications in environmental toxicology. Rapid identification of bacterial contaminants in water and environmental samples is essential for assessing toxic exposures, such as those caused by pathogenic contamination of drinking water. By lowering the time required to detect viable bacteria, the GO–resazurin system provides a sensitive and practical tool for evaluating environmental quality and potential health risks associated with microbial contamination. While the GO and resazurin lack interaction in the absence of cells, the S. aureus metabolism specifically increases electron shuttling of the probe towards the carbon material compared to other tested bacteria. Furthermore, with a comprehensive spectrofluorimetric characterization of resazurin/GO/bacteria interaction, we demonstrate an enhanced resorufin color loss that unveils potential applications in revolutionizing environmental monitoring, pollutant degradation, and microbial fuel cells design. These findings highlight the role of GO as a catalytic enhancer, enabling more sensitive early warning systems in exposure assessment, improving accuracy in pollution monitoring, and facilitating efficient and sustainable remediation strategies for toxic substances removal.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"101 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Molecular Alterations in the Gonads of Hoplias malabaricus Contaminated by Metals 金属污染马齿苋性腺的形态和分子变化

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-10-02 DOI: 10.1002/tox.24573

Breno Thuller, Yves Moreira Ribeiro, Elizete Rizzo, Nilo Bazzoli, Alessandro Loureiro Paschoalini

{"title":"Morphological and Molecular Alterations in the Gonads of Hoplias malabaricus Contaminated by Metals","authors":"Breno Thuller, Yves Moreira Ribeiro, Elizete Rizzo, Nilo Bazzoli, Alessandro Loureiro Paschoalini","doi":"10.1002/tox.24573","DOIUrl":"https://doi.org/10.1002/tox.24573","url":null,"abstract":"Mining is one of the most important activities in the economic development of several countries; however, it generates significant impacts on water resources, releasing metals in these environments. The exposure of fish to metals alters the redox capacity of the organism, increasing the production of reactive oxygen and nitrogen species, causing molecular and cellular changes. In 2019, the Paraopeba River basin, an important river in the Neotropical region, suffered from the discharge of tons of mining tailings, affecting the entire aquatic ecosystem. The present study analyzed cellular changes and their relationship with alterations in iNOS and metallothionein levels, caused by the accumulation of metals in the gonads of Hoplias malabaricus. Ten specimens were collected in a control environment and 10 specimens in contaminated Paraopeba River. For histopathological analysis, gonadal fragments were collected from all individuals and submitted to routine histological techniques. Additionally, metallothionein and iNOS were immunolabeled to evaluate oxidative stress and metals' influence. The results showed that several metals in the Paraopeba River led to histopathological alterations in the gonads of males and females. Furthermore, intense positive staining of metallothionein and iNOS in different reproductive cells was demonstrated. This study shows the effects of oxidative capacity caused by metals on the reproductive morphophysiology of fish from the Paraopeba River, even after 5 years since the dam rupture.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"37 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mono(2-Ethylhexyl) Phthalate Induces Inflammatory and Angiogenic Alterations Mediated by the PI3K/AKT Pathway in HTR-8/SVneo Trophoblastic Cells. 邻苯二甲酸单（2-乙基己基）酯通过PI3K/AKT通路诱导HTR-8/SVneo滋养细胞炎症和血管生成改变

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-26 DOI: 10.1002/tox.24570

Luis Daniel Martínez-Razo,Ericka Karol Pamela Almeida-Aguirre,Mariana Bobadilla-Bravo,Ismael Mancilla-Herrera,Alejandra Martínez-Ibarra,Marco Cerbón

{"title":"Mono(2-Ethylhexyl) Phthalate Induces Inflammatory and Angiogenic Alterations Mediated by the PI3K/AKT Pathway in HTR-8/SVneo Trophoblastic Cells.","authors":"Luis Daniel Martínez-Razo,Ericka Karol Pamela Almeida-Aguirre,Mariana Bobadilla-Bravo,Ismael Mancilla-Herrera,Alejandra Martínez-Ibarra,Marco Cerbón","doi":"10.1002/tox.24570","DOIUrl":"https://doi.org/10.1002/tox.24570","url":null,"abstract":"Exposure to mono(2-ethylhexyl) phthalate (MEHP) during pregnancy has been associated with adverse pregnancy and birth outcomes characterized by extravillous trophoblast (EVT) abnormal function. Previous reports have suggested that MEHP can activate the PI3K/AKT pathway in EVT cells, a pathway known to regulate inflammation and angiogenesis in these cells. However, the molecular effects of MEHP on crucial EVT functions such as inflammatory and angiogenic homeostasis remain unexplored. This study aimed to characterize the role of the PI3K/AKT pathway as a mechanism of action of MEHP activity, as well as its effects on inflammatory and angiogenic soluble molecules in HTR-8/Svneo EVT human-derived cells. The results showed that a low (5 μM) MEHP concentration increased AKT phosphorylation, but a high (200 μM) concentration did not. Conversely, a high MEHP concentration, but not a low concentration, promoted nuclear translocation of p65 in a PI3K-dependent manner. Notably, distinct patterns of cytokines were transcriptionally and secretorily activated by high and low concentrations of MEHP. IL1B, CXCL8, and TNF were transcriptionally upregulated by MEHP 5 μM, while gene expression and secretion of IL-6 were induced by MEHP 200 μM, suggesting a biphasic inflammatory dose response. In addition, both MEHP concentrations upregulated the expression of angiogenic molecules (VEGF, PGF, and ANGPTL4) and impaired migration and tube formation in HTR-8/Svneo cells. Both inflammatory and angiogenic responses induced by MEHP were inhibited by the PI3K inhibitor LY294002. Collectively, these data demonstrate that MEHP induces inflammation and impairs angiogenesis partly via PI3K/AKT in HTR-8/SVneo cells. These findings may help to understand previous clinical associations between MEHP exposure and placental pathophysiology.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"20 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxic Mechanisms of Fipronil in Human Astrocytes and the Protective Effect of Vitamin E on Fipronil-Induced Toxicity. 氟虫腈对人星形胶质细胞的毒性机制及维生素E对氟虫腈毒性的保护作用。

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-23 DOI: 10.1002/tox.24546

Ying-Tso Chen,Che-Sheng Chu,Wei-Chih Sun,Li-Min Chio,Wei-Zhe Liang

{"title":"Toxic Mechanisms of Fipronil in Human Astrocytes and the Protective Effect of Vitamin E on Fipronil-Induced Toxicity.","authors":"Ying-Tso Chen,Che-Sheng Chu,Wei-Chih Sun,Li-Min Chio,Wei-Zhe Liang","doi":"10.1002/tox.24546","DOIUrl":"https://doi.org/10.1002/tox.24546","url":null,"abstract":"Fipronil (FIP), a widely used agricultural insecticide, has raised significant concerns due to its harmful environmental residues and neurotoxic effects. Although FIP's toxicological impact has been studied in some neuronal cell models, its specific effects on human glial cells remain poorly understood. This study sought to investigate the mechanisms of FIP-induced toxicity and evaluate the protective potential of vitamin E (VE), a known antioxidant. Using human astrocyte (GHA) cells, we treated cells with FIP (5-25 μM), VE (20 μM), or both and compared their responses to untreated controls. Our findings revealed that FIP significantly reduced cell viability and caused morphological changes in astrocytes, including cellular shrinkage and detachment. FIP also increased the production of reactive oxygen species (ROS) and depleted intracellular glutathione (GSH) levels, indicating a disruption of cellular redox balance and the onset of oxidative stress. Furthermore, FIP triggered the activation of apoptotic pathways through upregulation of Bax, caspase-9, and caspase-3, coupled with downregulation of the antiapoptotic protein Bcl-2. Concurrently, FIP disrupted antioxidant defense mechanisms by modulating the Nrf2/HO-1/NQO1 signaling pathway. Interestingly, pretreatment with VE effectively reversed these effects. VE reduced ROS levels, replenished GSH, mitigated apoptosis, and restored antioxidant protein expression, protecting astrocytes against FIP-induced cytotoxicity and oxidative damage. These findings highlight oxidative stress as a critical factor in FIP-induced astrocyte toxicity and position VE as a promising protective agent. Further research is essential to explore VE's therapeutic potential in mitigating oxidative stress-related neurotoxicity caused by environmental toxins like FIP.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Safranal Against Paraquat-Induced Acute Lung Injury in Rat. Safranal对百草枯致大鼠急性肺损伤的保护作用。

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-20 DOI: 10.1002/tox.24561

Sima Beigoli,Arghavan Memarzia,Mahla Mohammadi Mahjoob,Nema Mohammadian Roshan,Mohammad Hossein Boskabady

{"title":"Protective Effects of Safranal Against Paraquat-Induced Acute Lung Injury in Rat.","authors":"Sima Beigoli,Arghavan Memarzia,Mahla Mohammadi Mahjoob,Nema Mohammadian Roshan,Mohammad Hossein Boskabady","doi":"10.1002/tox.24561","DOIUrl":"https://doi.org/10.1002/tox.24561","url":null,"abstract":"Lung and multi-organ injuries are caused by the herbicide paraquat (PQ). Safranal (Saf), the principal compound found in Crocus sativus, exhibits a diverse array of pharmacological properties, encompassing anti-inflammatory, anti-oxidative, and immunoregulatory activities. The potential preventive impacts of Saf and pioglitazone (Pio), in comparison to dexamethasone (Dexa), were investigated in rats to assess their efficacy in mitigating lung injury induced by PQ aerosol. Saline aerosol was given to the control (Ctrl) group of rats, whereas PQ aerosol was given to the other six groups (PQ), eight times every other day. PQ groups were given either saline (PQ), two doses of Saf (Saf (L), 0.8 mg/kg/day and Saf (H), 3.2 mg/kg/day), pioglitazone (Pio, 5 mg/kg/day), the combination of Saf (L) + Pio, or Dexa (0.03 mg/kg/day) during PQ exposure for 16 days. Superoxide dismutase (SOD), catalase (CAT), and thiol levels decreased, while malondialdehyde (MDA) in the bronchoalveolar lavage fluid (BALF), lung tissue levels of tumor necrosis factor (TNF-α) and interleukin 10 (IL-10), tracheal responsiveness (TR) to methacholine, and lung pathological changes were increased in the PQ group. Significant improvements were seen in all treated groups, with the exception of a few variables in Saf (L). The combination of Saf (L) + Pio had a greater effect than Saf (L) or Pio alone. All comparisons had p values < 0.05 to < 0.001. The Saf and Pio show promising combined preventative abilities, suggesting that the PPARγ receptor plays a role in Saf impact.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"18 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecotoxicological Evaluation of the Antimicrobial Butylparaben in Edaphic Organisms Using Multiple Biomarkers. 利用多种生物标志物评价对羟基苯甲酸丁酯在土壤生物中的生态毒理学作用。

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-20 DOI: 10.1002/tox.24568

Lorena Maihury Santos Tsubouchi,Edson Araújo de Almeida,Diane Scapin,Anna Karolina Gomes Oliveira,Cassiano Aparecido de Souza,Diego Espirito Santo,Carmem Lúcia Henrich,Ana Elisa Maehashi,Gideã Taques Tractz,Craig Allan Downs,Osvaldo Valarini Junior,Regiane da Silva Gonzalez,Elisângela Düsman,Ana Paula Peron

{"title":"Ecotoxicological Evaluation of the Antimicrobial Butylparaben in Edaphic Organisms Using Multiple Biomarkers.","authors":"Lorena Maihury Santos Tsubouchi,Edson Araújo de Almeida,Diane Scapin,Anna Karolina Gomes Oliveira,Cassiano Aparecido de Souza,Diego Espirito Santo,Carmem Lúcia Henrich,Ana Elisa Maehashi,Gideã Taques Tractz,Craig Allan Downs,Osvaldo Valarini Junior,Regiane da Silva Gonzalez,Elisângela Düsman,Ana Paula Peron","doi":"10.1002/tox.24568","DOIUrl":"https://doi.org/10.1002/tox.24568","url":null,"abstract":"Butylparaben (BuP) recurrently contaminates soils worldwide, mainly by incorporating sewage sludge into cultivated areas, using wastewater in irrigation, and leaching contaminated soils. However, there are few studies on the ecotoxicological effects of this paraben on edaphic organisms. The ecotoxicity of BuP was evaluated in seeds of Daucus carota, Allium cepa, and Cucumis sativus, in the roots of A. cepa bulbs, and in Eisenia fetida earthworms, at concentrations of 10, 50, 100, and 500 ng/L. In root meristems, the four concentrations of BuP induced lipid peroxidation and raised the levels of superoxide radicals, which triggered inhibition of cell division and mitotic spindle alteration, significantly reducing the growth of roots in seeds and bulbs. In animals, BuP at 10, 50, 100, and 500 ng/L caused 80%, 80%, 70%, and 90% evasion of earthworms from artificial soil, respectively. In addition, this paraben did not cause mortality in earthworms after 14 days of exposure. However, all concentrations increased the production of superoxide and hydroxyl radicals in cells and caused lipid peroxidation. Thus, increased exposure to this compound can affect the ecological functions negatively and/or cause the death of these animals. Therefore, recurrent contamination with BuP can negatively impact soil quality, posing a risk to agricultural productivity and the environment. This study is a pioneer in the ecotoxicological evaluation of BuP in plants at environmentally relevant concentrations and in the behavioral and oxidative stress study in earthworms.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"6 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2-Ethyl-Hexyldiphenyl Phosphate (EHDPP) Induces Cytotoxicity by Activation of Autophagy Process in HepG2 Cells. 2-乙基己基二苯基磷酸（EHDPP）通过激活HepG2细胞自噬过程诱导细胞毒性。

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-19 DOI: 10.1002/tox.24567

Mingrong Qian,Juntao Wang,Caihong Wang,Yuanxiang Jin

{"title":"2-Ethyl-Hexyldiphenyl Phosphate (EHDPP) Induces Cytotoxicity by Activation of Autophagy Process in HepG2 Cells.","authors":"Mingrong Qian,Juntao Wang,Caihong Wang,Yuanxiang Jin","doi":"10.1002/tox.24567","DOIUrl":"https://doi.org/10.1002/tox.24567","url":null,"abstract":"Organophosphate flame retardants (OPFRs) are extensively used in the electronics industry, plastics, textiles, and other sectors to reduce flammability and enhance fire safety. However, excessive OPFR use has led to significant environmental accumulation, increasing ecological stress and potential human health risks. This study investigates the effects of 2-ethylhexyldiphenyl phosphate (EHDPP) on HepG2 cells, demonstrating that EHDPP exposure impairs normal cellular function through excessive autophagy, ultimately reducing cell viability. Our findings reveal a concentration-dependent decline in cell survival, with an LC50 of 120 μM. While lower concentrations (0, 1, 5, 10, and 50 μM) exerted minimal effects, 50 μM EHDPP significantly reduced cell viability. Further analysis confirmed that EHDPP exposure upregulates autophagy-related genes (ATG5, ATG12, VPS34, Beclin1, FIP200, ATG13, and ATG3), leading to an increased LC3-II/I ratio and a decrease in p62 levels, indicative of enhanced autophagic activity. Transmission electron microscopy (TEM) imaging confirmed a substantial increase in autophagosome formation at 50 μM EHDPP. These results provide new insights into the cytotoxic mechanisms of EHDPP, emphasizing its role in triggering excessive autophagy in hepatic cells.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"55 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Association to Mechanism: Excessive Exposure to Tin During Pregnancy May Cause Fetal Neural Tube Defects 从关联到机制：孕期过量接触锡可能导致胎儿神经管缺陷

IF 4.5 3区医学

Environmental Toxicology Pub Date : 2025-09-02 DOI: 10.1002/tox.24566

Yongyan Chen, Yingnan Guo, Jing Wei, Chengrong Wang, Jufen Liu, Lei Jin, Zhiwen Li, Aiguo Ren, Linlin Wang

{"title":"From Association to Mechanism: Excessive Exposure to Tin During Pregnancy May Cause Fetal Neural Tube Defects","authors":"Yongyan Chen, Yingnan Guo, Jing Wei, Chengrong Wang, Jufen Liu, Lei Jin, Zhiwen Li, Aiguo Ren, Linlin Wang","doi":"10.1002/tox.24566","DOIUrl":"https://doi.org/10.1002/tox.24566","url":null,"abstract":"Despite widespread human exposure to tin, its health effects remain poorly understood. This study examined the role of tin in neural tube defects (NTDs) through a case–control study and animal experiments. Tin levels in maternal serum and placentas were analyzed in 200 NTD cases and 400 controls to explore potential associations. Elevated tin concentrations in maternal serum were associated with an increased risk of NTDs, with an odds ratio of 2.31 (95% CI, 1.13–4.75), with similar associations found for placental tin and tributyltin (TBT) exposure. In animal experiments, pregnant mice exposed to 10–40 mg/kg TBT exhibited a 25.5%–27.6% incidence of fetal NTDs. Maternal TBT exposure increased oxidative stress and apoptosis in embryonic neural tissues. Antibody microarray analysis prioritized MAPK signaling as the dominant perturbed pathway. Subsequent western blot and RT‐qPCR analysis convergently validated TBT‐induced MAPK hyperactivation. Vitamin E supplementation had antagonistic effects, reducing these harmful outcomes. These findings suggest that prenatal tin exposure is a significant risk factor for NTDs. The teratogenic effect of TBT appears to be mediated by enhanced oxidative stress, activation of MAPK signaling, and apoptosis in the developing neural tube, processes that can be mitigated by Vitamin E supplementation. Thus, tin exposure during pregnancy is associated with an increased risk of fetal NTDs, and animal models demonstrate that TBT can induce these defects through specific biological pathways. This research highlights the need for further investigation into tin exposure and its potential health impacts on fetal development.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"45 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144930771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Glyphosate on Zebrafish (Danio rerio) Developmental Toxicity. 草甘膦对斑马鱼发育毒性的影响。

IF 3.2 3区医学

Environmental Toxicology Pub Date : 2025-09-01 Epub Date: 2025-04-02 DOI: 10.1002/tox.24503

Ning Zhou, Ruirui Li, Jin Lu, Jiagao Cheng, Wenping Xu, Liming Tao, Yang Zhang

{"title":"Effects of Glyphosate on Zebrafish (Danio rerio) Developmental Toxicity.","authors":"Ning Zhou, Ruirui Li, Jin Lu, Jiagao Cheng, Wenping Xu, Liming Tao, Yang Zhang","doi":"10.1002/tox.24503","DOIUrl":"10.1002/tox.24503","url":null,"abstract":"<p><p>Glyphosate has been widely used in agricultural production as a highly effective, low-toxic, broad-spectrum organophosphorus herbicide. However, there has been controversy about whether it is toxic to the nervous system. In order to explore this issue in depth, the present study analyzed the molecular mechanism of action of glyphosate from four perspectives, namely, gene regulation, protein expression, morphological changes, and behavioral changes, and assessed the potential effects of glyphosate on the development of the nervous system of zebrafish through the establishment of a zebrafish model. The results showed that zebrafish embryos at 6 hpf after fertilization were exposed to glyphosate until 72 and 120 hpf. After exposure, it was found that the central nervous development-related gene Elavl3 was down-regulated, and GAP-43, Neurog1, and GFAP were up-regulated. The expression of HuC protein, which is used to maintain neuronal axonal homeostasis, was significantly reduced, and the expression of GFAP protein, which is used to repair neurological damage and inflammation, was significantly increased. Under the regulation of related genes and proteins, zebrafish larvae show abnormal changes during the development of a series of nervous systems such as heart rate slowing, somite shortening, spinal and brain malformations. At the same time, the zebrafish's action behavior also changed, with a significant decrease in its total time share in the low-speed shift and high-speed shift states, and a delayed response to dark-light environmental stimuli. In summary, studies have shown that glyphosate exposure may induce damage and inflammation of the zebrafish nervous system, resulting in developmental malformations, abnormal motor behavior, and potential neurotoxicity. Therefore, the possible neurotoxicity and environmental risks of glyphosate to aquatic animals should not be ignored and should be of great concern.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":"1160-1172"},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0