Environmental Toxicology最新文献

{"title":"Features of Microplastics in Seawater, Sediment, and Most Tourists Consumed Marine Fish and Shellfish From Cox's Bazar and Saint Martin's Island, Bay of Bengal, Bangladesh.","authors":"Md Azharul Islam Palash, Farjana Akter, Prianka Kundu, Md Abid Hasan, Shilpi Saha, S M Mahatab Uddin, Md Mishfikur Rahman Sunji, Anirban Sarker, Md Firoz Khan, Tahmina Begum, Mohammad Abdul Baki","doi":"10.1002/tox.70071","DOIUrl":"10.1002/tox.70071","url":null,"abstract":"<p><p>The present study investigated microplastic (MP) contamination in surface seawater, intertidal sediments, and commercially important fish and shellfish from Cox's Bazar and Saint Martin's Island, two of the most popular tourist destinations in Bangladesh. Seawater and sediment samples were collected from three locations per site, with each site having three replicates. Additionally, seven fish and four crustacean species were obtained from local markets and barbecue shops. MPs were morphologically identified under a stereomicroscope and chemically characterized using Fourier transform infrared spectroscopy (FTIR). The average MP concentrations in surface water were higher at Saint Martin's Island (35.1 ± 29.5 particles L^-1) than at Cox's Bazar (10.45 ± 7.65 particles L^-1). In sediments, concentrations averaged 0.211 ± 0.05 items g^-1 at Cox's Bazar and 0.2055 ± 0.1 at St. Martin's Islands. MP abundance in fish and shellfish was tissue-dependent and varied by location, generally following the order: gut > dorsal muscle > ventral muscle. Overall, mean MP concentrations in the corresponding tissues were higher in samples from Cox's Bazar than in those from St. Martin's Island. Fibers, predominantly black and red (0.1-0.5 mm), were the most dominant form of MPs across all samples. FTIR analysis identified polyethylene terephthalate (53.33%), polyethylene (26.67%), and polypropylene (20%) as major polymer types. The estimated daily MP intake via seafood consumption was highest in adults (74.99 items day^-1) and children (49.99 items day^-1), primarily through the ingestion of Awaous grammepomus . These findings revealed widespread MP contamination in the Bay of Bengal region, underscoring the potential ecological and human health risks associated with it.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":" ","pages":"1490-1507"},"PeriodicalIF":3.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147510569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Trigonella foenum‐graecum Extract Against Lead Toxicity: Integrated Redox, Endocrine, and Spermatogenic Responses","authors":"Veysi Kayri, Emre Sahin, Mehmet Irmak, Ramazan Ozmen, Recep Hakki Koca, Besir Er, Ibrahim H. Ozercan, Kazim Sahin","doi":"10.1002/tox.70204","DOIUrl":"https://doi.org/10.1002/tox.70204","url":null,"abstract":"Lead (Pb) exposure remains an important environmental health concern because of its bioaccumulation and toxicity to multiple organs, including the liver, kidneys, and male reproductive system. This study evaluated the protective effects of a standardized <jats:italic> <jats:styled-content style=\"fixed-case\">Trigonella foenum‐graecum</jats:styled-content> L </jats:italic> . (fenugreek; TFG) seed extract against subchronic lead acetate‐induced toxicity in rats. An integrated experimental design assessed biochemical, oxidative, molecular, endocrine, histopathological, and sperm functional parameters. Animals were assigned to control, TFG extract (50 or 100 mg/kg), lead acetate, and combined lead plus TFG extract groups. Lead exposure caused hepatorenal dysfunction and histopathological injury, accompanied by increased malondialdehyde (MDA), reduced antioxidant defenses, increased nuclear factor kappa B (NF‐κB) protein expression, and reduced nuclear factor erythroid 2‐related factor 2 (Nrf2) and heme oxygenase‐1 (HO‐1) protein expression. Lead exposure also decreased circulating follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) concentrations and impaired sperm motility, density, morphology, viability, and testicular architecture. Coadministration of the TFG extract significantly attenuated these alterations, improved biochemical and antioxidant indices, reduced NF‐κB expression, increased Nrf2 and HO‐1 expression, improved gonadotropin concentrations and sperm quality, and ameliorated hepatic and testicular histopathology. For most endpoints, the 100 mg/kg/day dose produced greater numerical improvement than 50 mg/kg/day, although between‐dose differences were not consistently significant. These findings indicate that the chemically characterized fenugreek extract attenuates lead‐induced systemic and reproductive toxicity in this preclinical rat model, with effects associated with improved redox balance, inflammation‐related protein expression, gonadotropin concentrations, and spermatogenic outcomes. Further studies are required before therapeutic or translational conclusions can be drawn.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"73 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149218365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Glutathione S‐Transferase and Metallothionein Polymorphisms on Mercury Levels and End‐Stage Renal Disease ( ESRD ) in Colombian Patients","authors":"Diana M. Narváez, Juliana Lago, Carlos Trillos, Marcela Varona, Leonardo Briceño, Ruth Marien Palma, Sonia M. Díaz, Gilma Hernández, Eliana M. Téllez, Juan Sebastián Aguilar, Daniela Castellanos, Xavier Martínez, Estefanía Zapata, Alix Rocio Barrios, Ignacio Villanueva, Michael R. Fonseca, Helena Groot","doi":"10.1002/tox.70196","DOIUrl":"https://doi.org/10.1002/tox.70196","url":null,"abstract":"Epidemiological studies have demonstrated a link between chronic kidney disease (CKD) and environmental mercury (Hg) exposure. Colombia has a well‐documented history of artisanal and small‐scale gold mining, where Hg exposure affects miners and nearby riverside and urban populations. This study aimed to evaluate the effects of polymorphisms in <jats:italic>GSTM1</jats:italic> , <jats:italic>GSTT1</jats:italic> , <jats:italic>MT1A</jats:italic> (rs8052394), and <jats:italic>MT2A</jats:italic> (rs10636) on Hg levels and their relationship with end‐stage renal disease (ESRD) in a Colombian urban population. We conducted a cross‐sectional, multicenter exploratory study on 401 CKD patients to assess the impact of polymorphisms on total Hg (tHg) levels in blood and hair, and their relationship with ESRD. Patients were divided into two groups: Group 1 (G1) with patients in Stages 1–4 CKD, and Group 2 (G2) with patients in Stage 5 CKD. Unexpectedly, tHg levels were low in our population (GM (SD) = 0.69 (2.07) and 0.73 (2.18) μg/L in blood, and GM (SD) = 0.22 (3.80) and 0.18 (2.9) μg/g in hair for G1 and G2, respectively). We found that low hair tHg levels were significantly associated with null <jats:italic>GSTT1</jats:italic> , double null <jats:italic>GSTM1/GSTT1</jats:italic> , and <jats:italic>MT1A</jats:italic> (rs8052394) GG genotype in G1, and high tHg with the null <jats:italic>GSTM1</jats:italic> genotype in G2 patients. Furthermore <jats:italic>,</jats:italic> when pooling G1 and G2, the double‐null genotype of <jats:italic>GSTM1</jats:italic> / <jats:italic>GSTT1</jats:italic> , as well as <jats:italic>MT1A</jats:italic> (rs8052394) AA and <jats:italic>MT2A</jats:italic> (rs10636) GC, were more prevalent among patients with hair and blood tHg levels exceeding 1 μg/g and 5 μg/L, respectively. Patients carrying the <jats:italic>MT1A</jats:italic> (rs8052394) AG genotype exhibited an increased risk of ESRD. In conclusion, our findings suggest that, even in a population with low Hg exposure, polymorphisms in glutathione S‐transferases and metallothioneins showed stage‐specific associations with hair tHg levels, and that <jats:italic>MT1A</jats:italic> may contribute to ESRD risk.","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"49 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Mechanism of Crocin Against Zearalenone-Induced Oxidative Damage to Swine Testicle Cells","authors":"Chu Yang,&nbsp;Rui Cao,&nbsp;Chao Li,&nbsp;Chongfan Du,&nbsp;Tianfang Xiao,&nbsp;Ruiyi Lin,&nbsp;Weimin Lin","doi":"10.1002/tox.70080","DOIUrl":"10.1002/tox.70080","url":null,"abstract":"<div>\u0000 \u0000 <p>Zearalenone (ZEA), a mycotoxin produced in moldy feed, induces oxidative damage in testicular cells of male animals. These cells are crucial for androgen secretion and male reproductive health. Consequently, identifying effective and safe treatments to maintain testicular redox balance and ensure male animal fertility is imperative. Crocin, a natural compound with recognized antioxidant properties, shows promise; however, its mechanism for mitigating ZEA-induced testicular oxidative injury remains unclear. To elucidate crocin's action on oxidative stress and apoptosis in swine testicular (ST) cells, we treated cells with crocin after establishing a ZEA-induced oxidative stress model. Compared to ZEA treatment alone, crocin significantly ameliorated oxidative stress by inhibiting reactive oxygen species (ROS) and malondialdehyde (MDA) elevation, while promoting the activity/levels of catalase (CAT), total antioxidant capacity (T-AOC), and superoxide dismutase (SOD). Furthermore, crocin effectively alleviated ZEA-induced apoptosis by attenuating mitochondrial membrane potential (MMP) depolarization, reducing the apoptosis rate, suppressing the expression of key apoptotic genes caspase 3 (<i>CASP3</i>) and caspase 9 (<i>CASP9</i>), and improving ZEA-induced G1 phase cell cycle arrest. Mechanistically, crocin mitigated oxidative damage at both mRNA and protein levels by restoring the homeostasis of the nuclear factor erythroid 2-related factor 2 (Nrf2) and mitogen-activated protein kinase (MAPK) signaling pathways, which were dysregulated by ZEA. In conclusion, crocin protects ST cells against ZEA-induced oxidative damage by reducing oxidative stress markers, alleviating ZEA-induced dysregulation of the Nrf2 and MAPK pathways, preventing apoptotic damage, and enhancing cellular antioxidant capacity. This study provides theoretical support for developing crocin as a potential feed additive to prevent oxidative damage to the boar reproductive system.</p>\u0000 </div>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1364-1373"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147439270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure and Bioaccumulation of Mercury Impacted the Extent of Cellular Damage and Oxidative Stress in Local Chickens Around Ilesha Gold Mining Areas of Nigeria","authors":"Bolaji F. Oyeyemi,&nbsp;Paul Ayodeji Awonegan,&nbsp;Adekunle Wahab Oyeyemi,&nbsp;Olatunji Abubakar Jimoh,&nbsp;John Babatope Owabumoye,&nbsp;Abiodun O. Fatoye,&nbsp;Olugbenga Samson Onile,&nbsp;Abass Toba Anifowoshe,&nbsp;Peter Olusakin Oladoye","doi":"10.1002/tox.70078","DOIUrl":"10.1002/tox.70078","url":null,"abstract":"<div>\u0000 \u0000 <p>Mercury (Hg) is a well-known global pollutant of environmental concern. Uncontrolled mining activities represent an avenue for its release and possible environmental contamination. Ilesha gold mining areas are characterized by extensive artisan and small-scale gold mining (ASGM) activities. However, it is largely unknown to what extent Hg pollution has occurred as well as the environmental and ecological deterioration in the area. Here, we studied Hg in soil, sediments, water, chicken blood, liver, muscle (chest), and kidney. Oxidative stress, hepatorenal damage, heat shock response, cellular DNA damage, and mRNA levels of apoptosis-related genes were analyzed using qPCR. All analyses were done in chickens from gold mining (GMA) and non-gold mining areas (NGMA). This study reported that the levels of Hg in soil (range: 1.637 ± 0.237–1.796 ± 0.104 μg/g), sediment (range: 1.424 ± 0.046–1.450 ± 0.062 μg/g), and water (range: 1.255 ± 0.071–1.378 ± 0.145 μg/mL) in GMA far exceeded Hg levels in NGMA. This might imply the extent to which ASGM activities have contributed to the Hg contamination in the areas. Chickens from GMA exhibited significantly high Hg levels, with the largest proportion bioaccumulated in the kidney (range: 0.596 ± 0.057–0.605 ± 0.028 μg/g) and lowest proportion in blood (range: 0.215 ± 0.023–0.226 ± 0.043 μg/g), suggesting the health risks dwellers in the area are prone to. Cellular damage biomarkers were significantly elevated in the tissue of Hg-exposed chickens from GMA compared to those from NGMA, demonstrating the significant health risks associated with Hg pollution and/or uptake in the areas.</p>\u0000 </div>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1403-1415"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147454603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan/Tripolyphosphate Nanoparticles Encapsulating Essential Oils as a New Class of Biopesticides: Structural Properties and Ecotoxicity Evaluation","authors":"Sofia Machado,&nbsp;Catarina Ganilho,&nbsp;Tatiana Andreani,&nbsp;Rose Marie O. F. Sousa,&nbsp;Artur Ribeiro,&nbsp;Ruth Pereira","doi":"10.1002/tox.70072","DOIUrl":"10.1002/tox.70072","url":null,"abstract":"<div>\u0000 \u0000 <p>Essential oils (EO) are fragrant, hydrophobic natural products derived from aromatic and medicinal plants that can be highly sensitive to environmental conditions. Nanoencapsulation offers a promising solution to overcome environmental degradation by abiotic factors. In this work, ionic gelation was employed to synthesize formulations of EO extracted from \u0000 <i>Satureja montana</i>\u0000 , \u0000 <i>Thymus vulgaris</i>\u0000 , \u0000 <i>Anethum graveolens</i>\u0000 , and \u0000 <i>Coriandrum sativum</i>\u0000 encapsulated in chitosan/TPP (tripolyphosphate) nanoparticles. Despite their potential as biopesticides, ecotoxicological aspects have not been much explored. Herein, aquatic (<i>Aliivibrio fisheri</i>, <i>Raphidocelis subcapitata</i>, \u0000 <i>Lemna minor</i>\u0000 , and \u0000 <i>Daphnia magna</i>\u0000 ) and terrestrial (\u0000 <i>Folsomia candida</i>\u0000 and soil microbial activity) non-target organisms were used to assess the environmental impact of the EO encapsulated in chitosan/TPP nanoparticles. The chitosan nanoparticles had a positive charge, high homogeneity (PDI &lt; 0.2), an average size of about 200 nm, and showed encapsulation efficiencies between 82% and 98%. Regarding hazard assessment, EO in their free form can cause toxic effects, even though they are prone to degradation by environmental factors. Encapsulation into chitosan/TPP nanoparticles resulted in a significant reduction of the essential oils' toxic profile. Overall, the observed toxicity comes mainly from the EO activity toward the aquatic test species. By reducing the ecotoxicity of EO toward non-target species (except for collembolans), the nanoformulations proved a promising solution to be explored as biopesticides, aiming to replace the use of synthetic pesticides. Only the nanoencapsulated \u0000 <i>C. sativum</i>\u0000 EO caused an inhibitory effect on nitrogen mineralization and arylsulfatase activity. Moreover, enhancing our understanding of the ecotoxicological impact of nanobiopesticides is essential before recommending their use in crop protection.</p>\u0000 </div>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1374-1402"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multistressor Interaction of Perfluorooctane Sulfonate (PFOS) and Temperature in Two Estuarine Fish Species, Red Drum (Sciaenops ocellatus) and Sheepshead Minnow (Cyprinodon variegatus)","authors":"Anna L. Thornton,&nbsp;Katy W. Chung,&nbsp;Peter B. Key,&nbsp;Emily C. Pisarski,&nbsp;Philip Tanabe,&nbsp;Marie E. DeLorenzo","doi":"10.1002/tox.70058","DOIUrl":"10.1002/tox.70058","url":null,"abstract":"<p>Perfluorooctane sulfonate (PFOS), a perfluoroalkyl substance (PFAS), is characterized by a long, fluorinated carbon chain which makes it ideal for oil- and water-repellent products but detrimental for the environment, where it persists and accumulates in organisms. PFOS has been detected in coastal ecosystems, but information on impacts to estuarine fish health and survival is lacking. In addition, potential rising temperatures due to climate change may alter organism physiology and bioavailability of chemical contaminants. This study examines the effects of PFOS on two species of fish found in Southeastern United States estuaries, the recreationally important red drum (<i>Sciaenops ocellatus</i>) and the standard toxicity test species sheepshead minnow (<i>Cyprinodon variegatus</i>), under two different temperatures (20°C and 30°C). The combined stress of PFOS exposure at the higher temperature yielded significantly greater chemical uptake and mortality in both species compared to PFOS at 20°C. The enhanced effect of temperature on PFOS mortality was greatest for \u0000 <i>C. variegatus</i>\u0000 . The combined stress of PFOS exposure and elevated temperature caused the greatest change in gene expression in \u0000 <i>C. variegatus</i>\u0000 relative to the individual stressors. Similarly, routine metabolic rates for \u0000 <i>S. ocellatus</i>\u0000 were significantly higher when exposed to the combined stress of PFOS exposure and elevated temperature. The results indicate that elevated temperature and PFOS act together to exacerbate toxicity in these estuarine fish. Sublethal responses to PFOS and temperature observed in this study could have long-term repercussions for fisheries. This study demonstrates the importance of expanding species used in ecological risk assessments and creating applicable results for wild species.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1167-1178"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/tox.70058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147270067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenols and Phthalates in Bottled Mineral Water: First Evidence of Co-Occurrence, Estrogenic Activity, and Health Risk in Brazil","authors":"Giulia Boito Reyes,&nbsp;Lucas Correia Gonçalves,&nbsp;Fernanda Cristina Iba Molinari,&nbsp;José Eduardo Gonçalves","doi":"10.1002/tox.70063","DOIUrl":"10.1002/tox.70063","url":null,"abstract":"<p>Bisphenol A (BPA), its structural analogs, and phthalates are synthetic chemicals widely used, with documented endocrine-disrupting activity. This study aimed to develop and validate a selective multiresidue analytical method for the simultaneous determination of BPA, 10 bisphenol analogs, and six phthalates in PET-bottled mineral water. To our knowledge, this is the first report assessing the co-occurrence of these contaminants in bottled mineral water in Brazil. Six commercial bottled water brands were sampled under two storage conditions: ambient temperature and solar exposure. Solid-phase extraction (SPE) was applied before GC–MS analysis. Among the 17 target analytes, BPA, BPF, BPS, and DIOP were detected in quantifiable concentrations. BPF and DIOP were found in all samples, with maximum concentrations of 7.92 and 3.85 μg L⁻¹, respectively. BPA and BPS were detected in specific brands only after sunlight exposure, reaching up to 7.10 and 9.08 μg L⁻¹, respectively. Despite concentrations being below current international regulatory limits, health risk assessment revealed that the estimated daily intake (EDI) of BPF and BPS resulted in safety factors (SF) below 1 for both adults and children, indicating a potential health concern. Estrogen equivalency (EEQ) values associated with BPA, BPF, BPS, and DIOP ranged from 0.5 to 13 ng E₂/L, exceeding effect-based trigger values proposed for estrogenic activity in bottled mineral water. The results suggest that even BPA-free labeled packaging may pose a risk due to the presence of its analogs and highlight the need for expanded regulatory oversight and routine monitoring of endocrine-disrupting compounds in bottled water.</p>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1179-1187"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/tox.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Shared Diagnostic Genes and Underlying Mechanisms Between Skin Exposure to Air Pollution and Skin Barrier Damage: A Bioinformatics Approach","authors":"Yueyue Chen,&nbsp;Liping Qu,&nbsp;Feifei Wang","doi":"10.1002/tox.70068","DOIUrl":"10.1002/tox.70068","url":null,"abstract":"<div>\u0000 \u0000 <p>Air pollution poses a significant threat to skin health, contributing to inflammation, aging, and disruption of the epidermal barrier. This study aims to identify genes associated with skin exposure to air pollution and skin barrier damage, and to discover potential biomarkers for these effects. Datasets related to air pollution-exposed skin (PS) and skin barrier damage (SD) were obtained from the Gene Expression Omnibus (GEO) database. GO and KEGG enrichment analyses were initially performed on both datasets. Through functional enrichment analysis, protein–protein interaction (PPI) network construction, and the application of two machine learning algorithms, we identified 140 common genes and two key diagnostic genes, FOSL1 and TKT. Receiver operating characteristic (ROC) curve analysis was used to validate the PS and SD datasets, achieving optimal area under the curve (AUC) values. Further investigation of FOSL1 and TKT via Gene Set Enrichment Analysis (GSEA) and immune cell infiltration analysis explored their roles in PS and SD conditions. Additionally, a skin model simulating air pollution exposure using particulate matters (PMs) was developed. RT-qPCR results showed that as the concentration of PMs increased, genes related to skin barrier damage were activated. The reliability of FOSL1 and TKT was confirmed through both RT-qPCR and Western Blot analyses.</p>\u0000 </div>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1249-1264"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147359079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEHP Exposure Affects Mitochondrial Function During Ovarian Follicle Development","authors":"Shu-Jie Yang,&nbsp;Xiao-Kang Lu,&nbsp;Li-Shu Li,&nbsp;Tong Tong,&nbsp;Jun-Yu Qian,&nbsp;Xing Duan","doi":"10.1002/tox.70060","DOIUrl":"10.1002/tox.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>The extensive utilization of plastics has resulted in the emergence of di(2-ethylhexyl) phthalate (DEHP) as a major contaminant in the environment, posing serious implications for human and animal health. Multiple investigations suggest that exposure to DEHP impairs female reproductive capacity, causing depletion of primordial follicles and disruption of hormone production. However, the specific mechanisms by which DEHP influences ovarian development and function in females remain unclear. In our work, we conducted an in vivo study using a mouse model exposed to 200 mg/kg DEHP for 28 days. We found that exposure to DEHP inhibited ovarian development and follicle maturation, leading to decreased numbers of primary and antral follicles. Furthermore, we observed that exposure to DEHP destroyed mitochondrial dynamics in the ovary, leading to mitophagy and autophagy. Additionally, DEHP exposure induced oxidative stress and abnormal mitochondrial energy metabolism by inhibiting Sirt3/Sod2-regulated signaling pathway in the ovary. Furthermore, our findings showed that DEHP exposure caused ovarian DNA damage and apoptosis by inhibiting Akt/mTOR signaling cascade. In conclusion, our study shows that DEHP exposure profoundly impairs ovarian function through inhibiting Sirt3/Sod2 and Akt/mTOR signaling pathways. These results provide valuable insights into the detrimental effects of DEHP on the female reproductive system.</p>\u0000 </div>","PeriodicalId":11756,"journal":{"name":"Environmental Toxicology","volume":"41 11","pages":"1213-1222"},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147304001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}