Engineering in Life Sciences最新文献

{"title":"Combination of Extrusion and Drop-on-Demand Bioprinting in One Process Enables the Local Placement of Cells or Signaling Factors Into (Bio) Printed Hydrogel Structures","authors":"Finn Dani,&nbsp;Nieves Cubo-Matteo,&nbsp;Leonie Schlicht,&nbsp;Michael Gelinsky,&nbsp;Anja Lode","doi":"10.1002/elsc.70062","DOIUrl":"10.1002/elsc.70062","url":null,"abstract":"<p>Combining the volumetric fabrication of hydrogel constructs using extrusion bioprinting with highly precise drop-on-demand (DoD) bioprinting offers exciting opportunities in biofabrication. This technical report presents a technique in which a solenoid micro-pipette is operated as an additional tool in an extrusion (bio)printing system to deposit small volumes of bioinks into extrusion-printed hydrogel constructs. Using three exemplary approaches, we show that this enables the patterned placement of cells or growth factors within 3D constructs and thus influences developmental processes. Human cells within low-viscosity bioinks, deposited into extrusion-printed hydrogel constructs by filling inter-strand cavities or by injection into the hydrogel strands, maintained their viability and functionality up to 28 days. As demonstrated for salivary gland cells, the properties of the hydrogel matrix can influence the fate of the injected cells: In a stiff alginate (Alg)-based hydrogel, they formed aggregates, which is beneficial for organoid formation, and in softer hydrogels, they migrated to neighboring cell clusters. Locally injected signaling factors such as vascular endothelial growth factor (VEGF) attracted endothelial cells and fibroblasts, which migrated into previously cell-free hydrogel areas. The combination of extrusion and DoD bioprinting opens new approaches to integrate different cell types and functionalizations in one construct, facilitating the creation of more complex and dynamic models.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12754084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145888709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148127013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148103121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Strategies for Fungal Cell Disruption in Biotechnological Applications","authors":"Bhagyeshri Ulhas Mantri,&nbsp;Maliheh Vahidinasab,&nbsp;Sonja Berensmeier","doi":"10.1002/elsc.70061","DOIUrl":"https://doi.org/10.1002/elsc.70061","url":null,"abstract":"<p>Fungal cell disruption plays a critical role in unlocking a wide range of high-value intracellular products such as lipids, proteins, pigments, and bioactive compounds. However, lysing fungal cells is far more challenging than breaking bacterial or algal cells due to their robust and highly structured cell walls. These biological barriers demand a tailored and strategic approach depending on the fungal species, cell morphology, and downstream processing requirements. This review explores the various mechanical and non-mechanical methods used to disrupt fungal cells, beyond outlining the core principles behind each method, the engineering and process factors that influence their performance are emphasized. A comparative analysis is provided, focusing on key parameters like disruption efficiency, scalability, cost-effectiveness, and environmental impact. The review also sheds light on emerging hybrid and integrated approaches, the role of pre-treatment or co-treatment strategies, and the potential for greener and more sustainable alternatives aligned with circular bioeconomy goals. Ultimately, this review aims to serve as a guide for researchers, bioprocess engineers, and industry professionals seeking to optimize fungal bioproduct extraction in a way that is not only technically sound but also economically viable and environmentally responsible, paving the way for more efficient, scalable, and sustainable fungal-based biomanufacturing.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145646409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148103120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scale-Up Strategy Focused on Hydrodynamic Stress for Mammalian Cell Culture Established by a Dry-Wet Approach","authors":"Hiroyuki Kenmoku,&nbsp;Akira Kaneko,&nbsp;Takanobu Saito,&nbsp;Takahiro Nemoto,&nbsp;Yoshikazu Kato,&nbsp;Shunsuke Ohira","doi":"10.1002/elsc.70054","DOIUrl":"https://doi.org/10.1002/elsc.70054","url":null,"abstract":"<p>Today, most recombinant protein drugs are produced by mammalian cells in a stirred-type bioreactor (BR). Although cell culture scale-up strategies have been extensively investigated, scale-up and switching BRs while maintaining comparable culture performance remains a challenging step. This is because the empirical correlations used to determine operating parameters are applicable only for limited situations using similar BRs across scales. In addition, a few small scale-down models (SSDMs) are able to evaluate cellular sensitivity to the shear environment of manufacturing-scale BRs. In this study, we focused on the hydrodynamic stress associated with agitation and developed an SSDM that generates high shear stress without undesirable secondary effects such as vortex formation and severe gas hold-up. In-house BRs with various scales and configurations were used for fed-batch culture of CHO-K1 cells, and their shear environment was characterized by computational fluid dynamics (CFD). Using the dry-wet approach, we found that average shear stress was well correlated with titer decrease as an indicator of culture performance. We also confirmed that the response to shear stress differs among cell lines, and that evaluation of the shear sensitivity of cells is accordingly a risk mitigation step that is required to ensure successful scale-up.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148117421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Chemical Defined Medium for Lactobacillus brevis Based on Artificial Intelligence","authors":"Chang Yu,&nbsp;Xu Yang,&nbsp;XiaoQing Ren,&nbsp;JianYe Xia","doi":"10.1002/elsc.70051","DOIUrl":"10.1002/elsc.70051","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p><i>Lactobacillus brevis</i> (<i>L. brevis</i>) is a bacterium known for its lactic acid production and probiotic properties. However, the limited research on industrial fermentation of <i>L. brevis</i> and its low productivity pose challenges. This work aimed to develop a cost-effective synthetic medium as an alternative to a complex medium for its large-scale production. We first investigated the nutrient requirements of <i>L. brevis</i> in terms of amino acids, vitamins, and nucleotides using the single nutrient omission method. Artificial intelligence algorithms combined with the Growth Profiler 960 (GP960) were then employed to optimize the concentration in the synthetic medium. The results indicate that <i>L. brevis</i> has diverse nutritional demands, including 18 essential amino acids (excluding aspartic acid and glutamine) and specific vitamins (pantothenic acid, nicotinic acid, vitamin B6, riboflavin) and nucleotides. Furthermore, the developed synthetic medium supported equivalent growth of <i>L. brevis</i> to that of the complex medium. The specific growth rate of <i>L. brevis</i> in synthetic medium could reach 0.297 h⁻¹. The cost of synthetic medium was 57.52% lower than that of fermentation medium. These results provide a solid foundation for further research on industrial fermentation of <i>L. brevis</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Summary</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Determine the requirements of <i>Lactobacillus brevis</i> growth for various types of amino acids, vitamins, and nucleotides.</li>\u0000 \u0000 <li>A combination of artificial intelligence algorithms and Growth Profiler 960 (GP960) equipment is used to optimize the medium at high throughput.</li>\u0000 \u0000 <li>No restrictions are placed on the factors and levels of medium optimization.</li>\u0000 \u0000 <li>Compared to complex medium, the components of the synthetic medium for <i>Lactobacillus brevis</i> are clearer and less expensive.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148113881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148129976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}