Endocrine regulations最新文献

{"title":"One-week sorghum (<i>Sorghum bicolor</i> L.) grain consumption is insufficient to increase adiponectin levels in prediabetic adults.","authors":"Sony Wibisono Mudjanarko, Teguh Rahardjo, Soebagijo Adi Soelistijo, Siti Rahmawati","doi":"10.2478/enr-2024-0002","DOIUrl":"10.2478/enr-2024-0002","url":null,"abstract":"<p><p><b>Objective.</b> Adiponectin is an internally produced bioactive compound with a protective role against the insulin resistance-related diseases. Finding an adiponectin modifier can play a beneficial role in preventing the progression of the diseases, particularly in the prediabetic patients, as a high-risk population. This study was undertaken to examine the effect of dietary sorghum grain for a week on the plasma adiponectin levels in prediabetic patients. <b>Methods.</b> The study involved 26 (13+13) participants in both control and intervention groups. The control group maintained their habitual diet of white rice, while the intervention group replaced their habitual diet of white rice with sorghum grain for seven consecutive days. In all participants, the adiponectin concentration was measured before and after the intervention period. <b>Results.</b> Most study subjects had central obesity and dyslipidemia. Adiponectin levels after the intervention period decreased from the baseline in the control and sorghum groups including in all BMI groups. The change of decreasing adiponectin level was greater in the control than the sorghum group and in line with greater BMI in the sorghum group, but statistically insignificant. No significant difference in adiponectin concentrations was found among BMI groups. <b>Conclusion.</b> Sorghum grain consumption for a week is insufficient to increase adiponectin levels in the prediabetic patients. Insulin resistance, central obesity, and dyslipidemia may be the confounding variables that alter the favorable effect of sorghum on adiponectin. Longer sorghum consumption or other interventions may be needed to increase the adiponectin levels in people under these conditions.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"58 1","pages":"11-18"},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of kisspeptin in the pathogenesis of a polycystic ovary syndrome.","authors":"Adiba Aasif, Roshan Alam, Haseeb Ahsan, Mohammad Mustufa Khan, Arshiya Khan, Saba Khan","doi":"10.2478/enr-2023-0032","DOIUrl":"10.2478/enr-2023-0032","url":null,"abstract":"<p><p>Hypothalamic-pituitary gonadal (HPG) axis is responsible for the development and regulation of the female reproductive system. In polycystic ovary syndrome (PCOS), there is a disturbance in the HPG axis. Kisspeptin, a neuropeptide produced by the KISS1 gene, plays a vital role in the regulation of HPG axis by binding with its receptors KISS1R/GPR54, and stimulates gonadotropin secretion from the hypothalamus into pituitary to release luteinizing hormone (LH) and follicle stimulating hormone (FSH). Polymorphisms or mutations in the KISS1 gene can cause disturbance in the kisspeptin signaling pathway and is thought to disrupt HPG axis. Altered signaling of kisspeptin can cause abnormal secretion of GnRH pulse, which leads to increased LH/FSH ratio, thereby affecting androgen levels and ovulation. The increased levels of androgen worsen the symptoms of PCOS. In the present article, we review the molecular physiology and pathology of kisspeptin and how it is responsible for the development of PCOS. The goal of this review article is to provide an overview and metabolic profile of kisspeptin in PCOS patients and the expression of kisspeptin in PCOS animal models. In the present article, we also review the molecular physiology and pathology of kisspeptin and how it is responsible for the development of PCOS.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"292-303"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138828830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicotinamide prevention in diabetes-induced alterations in the rat liver.","authors":"Tamara Kuchmerovska, Lesya Yanitska, Oksana Horkunenko, Mykhailo Guzyk, Tetiana Tykhonenko, Irina Pryvrotska","doi":"10.2478/enr-2023-0031","DOIUrl":"10.2478/enr-2023-0031","url":null,"abstract":"<p><p><b>Objective.</b> The study was performed to elucidate whether nicotinamide (NAm) can attenuate the diabetes-induced liver damage by correction of ammonia detoxifying function and disbalance of NAD-dependent processes in diabetic rats. <b>Methods.</b> After four weeks of streptozotocin-induced diabetes, Wistar male rats were treated for two weeks with or without NAm. Urea concentration, arginase, and glutamine synthetase activities, NAD+ levels, and NAD+/NADH ratio were measured in cytosolic liver extracts. Expression of <i>parp-1</i> gene in the liver was estimated by quantitative polymerase chain reaction and PARP-1 cleavage evaluated by Western blotting. <b>Results.</b> Despite the blood plasma lipid peroxidation products in diabetic rats were increased by 60%, the activity of superoxide dismutase (SOD) was reduced. NAm attenuated the oxidative stress, but did not affect the enzyme activity in diabetic rats. In liver of the diabetic rats, urea concentration and arginase activity were significantly higher than in the controls. The glutamine synthetase activity was decreased. Decline in NAD+ level and cytosolic NAD+/NADH ratio in the liver of diabetic rats was observed. Western blot analysis demonstrated a significant up-regulation of PARP-1 expression accompanied by the enzyme cleavage in the diabetic rat liver. However, no correlation was seen between mRNA expression of <i>parp-1</i> gene and PARP-1 protein in the liver of diabetic rats. NAm markedly attenuated PARP-1 cleavage induced by diabetes, but did not affect the <i>parp-1</i> gene expression. <b>Conclusions.</b> NAm counteracts diabetes-induced impairments in the rat liver through improvement of its detoxifying function, partial restoration of oxidative stress, NAD+ level, normalization of redox state of free cytosolic NAD+/NADH-couples, and prevention of PARP-1 cleavage.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"279-291"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138828829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population.","authors":"Andrii Prodan, Ihor Dzubanovsky, Oleksandr Kamyshnyi, Natalia Melnyk, Svitlana Pidruchna, Stanislava Voloshyn","doi":"10.2478/enr-2023-0030","DOIUrl":"10.2478/enr-2023-0030","url":null,"abstract":"<p><p><b>Objective.</b> Many conflicting results have been obtained in the study of leptin (LEP) and leptin receptor (LEPR) gene variants that are associated with the obesity and diabetes possibly due to differences in the study populations. The aim of this study was to evaluate changes in the metabolic hormones (leptin, ghrelin, adiponectin, resistin) levels in the blood of obese patients in relation to the GHRL (rs696217), LEP (rs7799039), LEPR (rs1137100, rs1137101, rs1805094) polymorphism in Ukrainian population. <b>Methods.</b> The study involved 53 obesity cases and 48 non-obesity subjects (controls). The GHRL, LEP, and LEPR genes polymorphism (rs696217, rs7799039, rs1137100, rs1137101, rs1805094) was genotyped using a TaqMan real-time polymerase chain reaction method. Blood hormones (leptin, ghrelin, adiponectin, resistin) were determined with commercially available kits using a Multiskan FC analyzer. <b>Results.</b> The study of the effect of genotypes of the GHRL (rs696217), LEP (rs7799039), and LEPR (rs1137100, rs1805094) polymorphisms on the level of metabolic hormones (leptin, ghrelin, adiponectin, resistin) in the blood of obese patients did not show reliably significant results. Thus, the presence of the LEPR genes (rs1137101) polymorphism in the Ukrainian population indicates an increased risk of the metabolic syndrome development regardless of the homozygous or heterozygous genotype (genotypes AA, AG, GG). <b>Conclusions.</b> We established a significant effect of the presence of the A allele and G allele of the LEPR gene polymorphism (rs1137101) on the level of leptin, ghrelin, adiponectin, and resistin in the serum of patients diagnosed with the metabolic syndrome in the Ukrainian population.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"269-278"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138828828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum adipokines (omentin-1 and visfatin) in coronary artery disease at a North Indian hospital.","authors":"Saif Ali, Roshan Alam, Mohammad Kaleem Ahmad, Mukhtar Ahmad, Haseeb Ahsan, Mohammad Mustafa Khan, Saba Khan","doi":"10.2478/enr-2023-0029","DOIUrl":"10.2478/enr-2023-0029","url":null,"abstract":"<p><p><b>Objective.</b> Adipose tissue is considered to be an endocrine organ that secretes bioactive substances known as adipokines that contribute to the pathophysiology of metabolic and coronary diseases related to obesity. In this study, various novel biomarkers, such as inflammatory markers that are pro-inflammatory (visfatin) and anti-inflammatory (omentin-1), as prognostic indicators for people with coronary artery disease (CAD) were investigated. <b>Methods.</b> In this study, 30 diabetic patients with CAD, 30 diabetic patients without CAD, and 30 healthy control counterparts were included. Serum omentin and visfatin concentrations were evaluated by solid-phase enzyme linked immunosorbent assay (ELISA) kit. Patients with established diagnosis of CAD based on angiography, ECG, and elevated cardiac marker level were included into the study. Patients with cardioembolic stroke, cerebral venous sinus thrombosis, CNS vasculitis, and hemorrhage due to trauma, tumor, vascular malformation, and coagulopathy were excluded. <b>Results.</b> The serum omentin-1 levels were significantly higher in the healthy controls in comparison with the diabetic group (p<0.0001) and serum visfatin levels were significantly higher in the diabetic group in comparison with the healthy controls (p<0.0001). The serum omentin levels were significantly higher in the diabetic group in comparison with the cardio-diabetic group (p<0.0001) and serum visfatin levels were significantly higher in the cardio-diabetic group in comparison with the diabetic group (p<0.0001). The serum omentin-1 showed negative correlation with the serum visfatin in the cardio-diabetic group. <b>Conclusion.</b> The adipokines, such as omentin and visfatin, may be good therapeutic candidates in preventing or ameliorating CAD.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"262-268"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138828827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia controls the expression of genes responsible for serine synthesis in U87MG cells on ERN1-dependent manner.","authors":"Myroslava Y Sliusar, Dmytro O Minchenko, Olena O Khita, Dariia O Tsymbal, Yuliia M Viletska, Olha Y Luzina, Serhij V Danilovskyi, Oksana O Ratushna, Oleksandr H Minchenko","doi":"10.2478/enr-2023-0028","DOIUrl":"10.2478/enr-2023-0028","url":null,"abstract":"<p><p><b>Objective.</b> Serine synthesis as well as endoplasmic reticulum stress and hypoxia are important factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown of ERN1 (endoplasmic reticulum to nucleus signaling) significantly suppressed the glioblastoma cell proliferation and modified the hypoxia regulation. The present study is aimed to investigate the impact of hypoxia on the expression of <i>PHGDH</i> (phosphoglycerate dehydrogenase), <i>PSAT1</i> (phosphoserine aminotransferase 1), <i>PSPH</i> (phosphoserine phosphatase), <i>ATF4</i> (activating transcription factor 4), and <i>SHMT1</i> (serine hydroxymethyltransferase 1) in U87MG glioblastoma cells in relation to knockdown of ERN1 with the intent to reveal the role of ERN1 signaling pathway on the endoplasmic reticulum stress-dependent regulation of expression of these genes. <b>Methods.</b> The control U87MG glioblastoma cells (transfected by empty vector) and ERN1 knockdown cells (transfected by dominant-negative ERN1) were exposed to hypoxia introduced by dimethyloxalylglycine for 4 h. RNA was extracted from cells and reverse transcribed. The expression level of <i>PHGDH</i>, <i>PSAT1</i>, <i>PDPH</i>, <i>SHMT1</i>, and <i>ATF4</i> genes was studied by real-time qPCR and normalized to ACTB. <b>Results.</b> It was found that hypoxia up-regulated the expression level of <i>PHGDH</i>, <i>PSAT1</i>, and <i>ATF4</i> genes in control U87MG cells, but <i>PSPH</i> and <i>SHMT1</i> genes expression was down-regulated. The expression of <i>PHGDH</i>, <i>PSAT1</i>, and <i>ATF4</i> genes in glioblastoma cells with knockdown of ERN1 signaling protein was more sensitive to hypoxia, especially <i>PSAT1</i> gene. At the same time, the expression of <i>PSPH</i> gene in ERN1 knockdown cells was resistant to hypoxia. The expression of <i>SHMT1</i> gene, encoding the enzyme responsible for conversion of serine to glycine, showed similar negative sensitivity to hypoxia in both control and ERN1 knockdown glioblastoma cells. <b>Conclusion.</b> The results of the present study demonstrate that the expression of genes responsible for serine synthesis is sensitive to hypoxia in gene-specific manner and that ERN1 knockdown significantly modifies the impact of hypoxia on the expression of <i>PHGDH</i>, <i>PSAT1</i>, <i>PSPH</i>, and <i>ATF4</i> genes in glioblastoma cells and reflects the ERN1-mediated reprograming of hypoxic regulation at gene expression level.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"252-261"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41195395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of leucocyte and platelet indices in patients with type 2 diabetes mellitus with microvascular complications at a tertiary care hospital in south India - A prospective cross-sectional study.","authors":"Raj Gokul, Chidambaram Yoganathan, Christopher Paul Clement Jenil Dhas, Nekkanti Abilash, Petchiappan Velammal, Kumar Bhargavi, Sivaraj Sujith Kumar","doi":"10.2478/enr-2023-0026","DOIUrl":"10.2478/enr-2023-0026","url":null,"abstract":"<p><p><b>Objective.</b> The present study was directed to assess the correlation between leukocyte and platelet indices and microvascular complications in patients with type 2 diabetes mellitus (T2DM). <b>Methods.</b> A prospective cross-sectional study was conducted between January 2020 and May 2021 at a tertiary healthcare center. Sixty T2DM patients, who fulfilled the inclusion and exclusion criteria, were included into the study and divided into 2 groups: T2DM patients with microvascular complications and T2DM patients without vascular complications. Clinical history was taken and examinations (routine complete blood count) were done to obtain platelet indices, neutrophillymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and lymphocyte-monocyte ratio (LMR) were obtained and tabulated. A correlation was statistically analyzed from the obtained data, p value <0.05 was considered to be statistically significant. <b>Results.</b> From the patients with microvascular complications, 18 cases suffered from retinopathy and nephropathy. Majority of the participants suffered from moderate non-proliferative retinopathy. The creatine median and absolute neutrophil count (ANC) were significantly higher in T2DM patients with microvascular complications (p<0.0001 and p<0.0054, respectively) compared to T2DM patients without vascular complications. No significant correlation was found between platelet indices, NLR, PLR with regard to fasting blood sugar, post prandial blood sugar, HbA1C in T2DM patients. <b>Conclusions.</b> Since no significant correlation was found between the different platelet indices and microvascular complications, it is evident that these markers cannot be used as the predictors of microvascular complications in T2DM patients.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"235-241"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41195394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of DNA methylation of <i>CAPN10</i> gene changes in the patients with type 2 diabetes mellitus as a predictive biomarker instead of HbA1c, random blood sugar, lipid profile, kidney function test, and some risk factors.","authors":"Harem Othman Smail, Dlnya Asaad Mohamad","doi":"10.2478/enr-2023-0025","DOIUrl":"10.2478/enr-2023-0025","url":null,"abstract":"<p><p><b>Objective.</b> Nowadays, type 2 diabetes mellitus (T2DM) is the most common chronic endocrine disorder, affecting an estimated 5-10% of adults worldwide and this disease rapidly increases in the Kurdistan region population. This research aims to identify DNA methylation change in the <i>CPAN10</i> gene as a predictive biomarker in T2DM and the association between DNA methylation status with lipid profile and kidney function test. <b>Methods.</b> The participants (113) were divided into three groups: diabetes group (47), prediabetes group (36), and control group (30). The study was carried out on patients who visited the private clinical sectors between August and December 2021 in the Koya city Kurdistan region of Iraq. To determine DNA methylation status, methylation-specific PCR (MPS) with paired primer for each methylated and unmethylated region was used. The Mann-Whitney U test and Spearman's correlation were performed for statistical analysis of data and a value of p<0.05 was considered significant. <b>Results.</b> The obtained results show that DNA hypermethylation was recorded in the promoter region in the samples of the diabetes and prediabetes groups compared to the healthy group (control). Various factors also affected the level of DNA methylation, such as HbA1c in prediabetes group and body mass index in the control group. <b>Conclusion.</b> These results indicate that DNA methylation changes in the <i>CAPN10</i> gene promoter region may be used as a potential predictive biomarker to diagnose T2DM; however, this study requires further data to support this evidence.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"221-234"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41195396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress in liver of streptozotocin-induced diabetic mice fed a high-fat diet: A treatment role of <i>Artemisia annua</i> L.","authors":"Mahshid Ghanbari, Mohammad Shokrzadeh Lamuki, Forouzan Sadeghimahalli, Emran Habibi, Mohammad Reza Sayedi Moqadam","doi":"10.2478/enr-2023-0027","DOIUrl":"10.2478/enr-2023-0027","url":null,"abstract":"<p><p><b>Objective.</b> The aim of this study was the investigation of a treatment role of Artemisia annua L. (AA) on liver dysfunction and oxidative stress in high-fat diet/streptozotocin-induced diabetic (HFD/STZ) mice. <b>Methods.</b> Sixty mice were divided into 12 groups including control, untreated diabetic, and treated diabetic ones with metformin (250 mg/kg), and doses of 100, 200, and 400 mg/kg of water (hot and cold) and alcoholic (methanol) extracts of AA. Type 2 diabetes mellitus (T2DM) was induced in mice by high-fat diet for 8 weeks and STZ injection in experimental animals. After treatment with doses of 100, 200 or 400 mg/kg of AA extracts in HFD/STZ diabetic mice for 4 weeks, oxidative stress markers such as malondialdehyde (MDA), glutathione (GSH), and free radicals (ROS) were determined in the liver tissue in all groups. <b>Results.</b> Diabetic mice treated with metformin and AA extracts showed a significant decrease in ROS and MDA concentrations and a notable increase in GSH level in the liver. Effectiveness of higher doses of AA extracts (200 and 400 mg/kg), especially in hot-water and alcoholic ones, were similar to and/or even more effective than metformin. <b>Conclusion.</b> Therapeutic effects of AA on liver dysfunction showed that antioxidant activity of hot-water and alcoholic AA extracts were similar or higher than of metformin.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"242-251"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41195397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the GHRL gene (rs696217) polymorphism on the metabolic disorders in patients with obesity in the Ukrainian population.","authors":"Andrii Prodan,&nbsp;Ihor Dzubanovsky,&nbsp;Oleksandr Kamyshnyi,&nbsp;Natalia Melnyk,&nbsp;Stepan Grytsenko,&nbsp;Stanislava Voloshyn","doi":"10.2478/enr-2023-0021","DOIUrl":"10.2478/enr-2023-0021","url":null,"abstract":"<p><p><b>Objective.</b> Over the past four decades, the prevalence of obesity has tripled and limited genetic studies with specific SNPs have been conducted, but no investigations using ghrelin and obestatin prepropeptide (GHRL) gene have been reported in the Ukrainians population. The aim of this study was to evaluate changes in the level of metabolic hormones in the blood of obese patients in relation to the GHRL (rs696217) polymorphism. <b>Methods.</b> The study involved 53 obesity cases and 48 non-obesity subjects (controls). The GHRL (rs696217) polymorphism was genotyped using a TaqMan real-time polymerase chain reaction method. Blood hormones were determined with commercially available kits using a Multi-skan FC analyzer. <b>Results.</b> Carriers of the T allele of the GHRL (rs696217) polymorphism were statistically significantly more in patients diagnosed with obesity compared to controls indicating a genetically determined cause of obesity. We also established a significant effect of the presence of the T allele of the GHRL (rs696217) polymorphism on the decrease in the adiponectin level and the increase of resistin level in obese patients. The study of the effect of genotypes (TT, GT, GG) of the GHRL (rs696217) polymorphism on the metabolic hormone levels in the blood of obese patients did not show reliably significant differences. <b>Conclusions.</b> The presence of the T allele of the GHRL (rs696217) polymorphism in Ukrainian population indicates an increased risk of the obesity development regardless on the homozygous or heterozygous genotype.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"173-182"},"PeriodicalIF":0.0,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10288257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}