Ina Ramírez Miranda, David Betancur Ancona, Yolanda Moguel Ordóñez
{"title":"Influence of phenolic compounds and flavonoids on the colour and antioxidant activity of Melipona beecheii honey from deciduous forest of Yucatan, Mexico","authors":"Ina Ramírez Miranda, David Betancur Ancona, Yolanda Moguel Ordóñez","doi":"10.9755/ejfa.2023.3198","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3198","url":null,"abstract":"The Melipona beecheii honey has bioactive compounds with high antioxidant activity that influence its colour. This research evaluated the influence of phenolic compounds and flavonoids on the colour and antioxidant activity of M. beecheii honeys. Forty-three honey samples from different site located in the low deciduous forest of Yucatan, Mexico, were extracted during the harvest and post-harvest seasons (2020 – 2021). The Pfund method was used to measure colour, Folin-Ciocalteu method for phenols measurement, aluminum chloride method for flavonoids determination, and ABTS and DPPH IC50 assay to determine antioxidants. The predominant colour of the honey was extra light amber and light amber at both seasons. Phenols ranged from 780 to 1317 mg/kg GAE; flavonoids from 36 to 55 mg/kg CE and antioxidant were 675 to 1161 µmol/kg TEAC by the ABTS and 361.8 to 173.0 mg/mL by the DPPH IC50. Phenols and flavonoids were correlated with colour and radical scavenging activity, indicating the influence of these compounds with the darker colour and higher antioxidant activity of honey. The statistically significant difference (P<0.05) between seasons could be associated with the botanical origin with great diversity of melliferous flora present in the low deciduous forest of the Yucatan Peninsula. Keywords: Antioxidant; colour; flavonoids; honey; Melipona beecheii.","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139161945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yali Zhang, Jing Gao, Rui Li, Yonggang Yan, Gang Zhang, Nan Wang, Wenbo Wang, Jiakun Yan
{"title":"Bacillus megaterium Compensates for Growth Inhibition from Phosphorus Deficiency by Improving Photosynthetic Capacity, Changing Antioxidant Potential, and Regulating Non-Structural Carbohydrate Concentrations in Glycyrrhiza uralensis","authors":"Yali Zhang, Jing Gao, Rui Li, Yonggang Yan, Gang Zhang, Nan Wang, Wenbo Wang, Jiakun Yan","doi":"10.9755/ejfa.2023.3200","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3200","url":null,"abstract":"This study assessed the effect of Bacillus megaterium on seedling growth of Glycyrrhiza uralensis Fisch. under control and phosphorus (P) deficiency conditions. The results showed that P deficiency improved 1) G. uralensis root growth, 2) superoxide dismutase, catalase, and peroxidase activities, 3) inorganic P, starch, and soluble sugar contents in roots, 4) dissipated energy flux per reaction center, trapped energy flux per reaction center, and absorption flux per reaction center, and 5) net photosynthetic rate, stomatal conductance, transpiration rate, maximum fluorescence intensity after dark adaptation, and variable fluorescence. However, P deficiency significantly decreased chlorophyll and carotenoid contents in G. uralensis, but enhanced chlorophyll and carotenoid contents in B. megaterium. Our findings on the regulatory mechanisms of B. megaterium in response to P starvation hold promise for improving the success of G. uralensis cultivation. Keywords: Bacillus megaterium; Glycyrrhiza uralensis Fisch; Phosphorus deficiency; Chlorophyll fluorescence; Antioxidant enzymes superoxide; Non-structural carbohydrate","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139163014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tri Sutriani Syam, Syahrijuita Kadir, Ika Yustisia
{"title":"Nata de durio: the utilization of durian seeds as a glucose source in the production of bacterial cellulose gel","authors":"Tri Sutriani Syam, Syahrijuita Kadir, Ika Yustisia","doi":"10.9755/ejfa.2023.3196","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3196","url":null,"abstract":"Durian seeds are a waste product from durian fruit. Durian seeds are often used by people as the main ingredient in making bread and cakes. However, the use of durian seeds as the main ingredient in making nata has never been done. Nata is extracellular cellulose obtained from the activity of the bacteria Acetobacter xylinum. The nata that is often found is nata de coco made from coconut water as the main ingredient. This research aims to look at the formation of nata de durio from durian seeds. The results showed that different dry weights of durian seeds did not affect the thickness of the Nata De Durio formed. The thickness of Nata De Durio on days 7 and 14 was highest at a dry weight of 250 grams of durian seeds with an average of 0.4 and 0.5 cm and the lowest was at a dry weight of 100 grams of durian seeds with an average of 0. 3 and 04 cm. Conclusion: The difference in dry weight of durian seeds does not affect the thickness of Nata De Durio. However, the dry weight of 250 gram durian seeds has a better Nata De Durio thickness compared to the dry weight of 100 grams and 200 grams of durian seeds. Keywords: Durian seeds, thickness, Nata De Durio","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139179684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of bioactive properties of Capparis spinosa fruits and use in production of Tulum cheese","authors":"A. Dinçoğlu, Ali Ileri, Jerina Rugji","doi":"10.9755/ejfa.2023.3179","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3179","url":null,"abstract":"The focus of the present study was to evaluate the bioactive properties of Capparis spinosa (caper) fruits and to investigate some quality parameters of Tulum cheese with addition of caper. Analyses for the phytochemical components of caper fruit used in the production of Tulum cheese showed that it has high levels of bioactive components in terms of antioxidant activity, total phenolics, and flavonoids. It was determined that C. spinosa, which is used in the production of Tulum cheese, had a good radical scavenging activity (DPPH radical assay). Four experimental groups were arranged in the present study and analyzed on the 1, 30, 45, 60, and 90th day of ripening. T_C was designed as the control group, T_C1, T_C2, and T_C4 groups contained 1, 2, and 4% of caper. According to the obtained results as the content of caper increased, dry matter ratios, ash, pH values, tyrosine and free fatty acid values in cheese increased significantly. The microbiological evaluation revealed that the caper affected positively Lactobacillus spp. counts. The highest aroma and taste scores among caper cheeses were given to the control group (T_C). Generally, caper did not have a negative effect on the textural profile of the product. These results revealed that C. spinosa contributes to the improvement of the bioactive potential as well as increasing the quality parameters of cheese. Keywords: Caper; Bioactive properties; Functional dairy; Microbiological properties; Tulum cheese","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rumella Simarmata, Ngadiman, Muhammad Saifur Rohman, Intan Chairun Nisa, Saraswati, Agus Rachmat
{"title":"Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity","authors":"Rumella Simarmata, Ngadiman, Muhammad Saifur Rohman, Intan Chairun Nisa, Saraswati, Agus Rachmat","doi":"10.9755/ejfa.2023.3182","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3182","url":null,"abstract":"Plant growth promoting bacteria with dual activity, 1-aminocyclopropane-1-carboxylic-acid deaminase (ACCD) and nitrogenase, is more effective in supporting plant growth under stress condition. Previously, we were obtained several endophytic bacterial strains that exhibited dual activity, one of which was Raoultella terrigena PCM8. This study aimed to characterize the ACCD and nitrogenase genes of PCM8 strain. The acdS gene was obtained from the results of Whole Genomic Sequencing analyis, while the nifH gene was obtained by PCR. The characterization of both of the genes was carried out by means of in-silico analysis. WGS annotation analysis, showed that the acdS gene of PCM8 was located at the locus 19090 of genomic DNA and contains 978 nucleotides. In silico analysis of both acdS and nifH gene products showed that the ACCD enzyme of PCM8 had 325 amino acids, with molecular weight of 34.95 kDa, while nitrogenase as represented by nifH subunit product consist of 96 amino acids with molecular weight of 93.98 kDa, respectively. The ACCD had pI value of 5.06, and catalytic residues of Lys51, Ser78, Tyr287, and Thr288, while nifH gene product had the pI value of 11.77. The results suggested that R. terrigena PCM8 potentially produce double activity of ACCD and nitrogenase and therefore it can be a good candidate as plant growth promoting under stress condition. Keywords: acdS gene, 1-aminocyclopropane-1-carboxylic acid deaminase, endophytic bacteria, nifH gene, nitrogenase Raoultella terrigena","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The impact on shelf life of Tulum cheese of modified atmosphere packaging","authors":"Pelin Demir, O. I. Ilhak, Gülsüm Öksüztepe","doi":"10.9755/ejfa.2023.3187","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3187","url":null,"abstract":"Tulum cheese is one of the most important conventional fermented dairy products produced in Türkiye. In this study, the effects of modified atmosphere packaging on the microbiological, physicochemical, sensory properties, and shelf life of Tulum cheese were investigated. For this aim, Tulum cheese samples were divided into four groups (B=100% CO2, C =100% N2, D=70% N2+30% CO2, E=75% N2+25% CO2), and the control samples were packaged in the air (A). These samples stored at 4±1°C were analyzed for the microbiological, chemical, and sensory on days 0, 30, 60, 90, 120, 150, 180, 210, and 240. TMAB, LLP, lactic streptococcus, lipolytic microorganisms, proteolytic microorganisms, and yeast-mold counts increased while the counts of coliform group bacteria, Enterobacteriaceae, and Staphylococcus aureus continually decreased in all groups during the storage. During the storage period, the E. coli and sulfate-reducing anaerobic bacteria counts were detected below detectable levels (<1.0 log10 CFU/g) in all groups. Other chemical parameter values increased continuously while pH values continually decreased during the storage in all the groups. It was observed that the total sensory scores of the samples decreased continually during the storage period. However, when the scores of the E group were evaluated within themselves, significant differences were obtained. In conclusion, Tulum cheese samples packaged in MAP showed significant changes in quality, and their shelf life was extended. Keywords: Modified atmosphere packaging; Quality characteristics; Sensory evaluation; Shelf-life extension; Tulum cheese","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Behera, Kasireddy Sivasankarreddy, B. J. Reddy, N. Saharia, Ramendra Nath Sarma, S. K. Singh, P. K. Majhi, Nayanmoni Borah
{"title":"Genetic gain and selection of stable genotypes in high zinc rice using AMMI and BLUP based stability methods","authors":"P. Behera, Kasireddy Sivasankarreddy, B. J. Reddy, N. Saharia, Ramendra Nath Sarma, S. K. Singh, P. K. Majhi, Nayanmoni Borah","doi":"10.9755/ejfa.2023.3180","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3180","url":null,"abstract":"Rice is the staple food of almost half of the world’s population, impacting nutrition especially in children, pregnant women, and nursing mothers. Because the traits were quantitatively inherited, they are affected by changes in location and year. A RBD with three replications was used to identify superior and stable high-zinc rice genotypes in Uttar Pradesh, India. Grain zinc content (GZC) is negatively correlated with grain yield using genetic association study. There was a significant G × E interaction (GEI) and V16 and V21 for GYP and V9, V2 and V10 for GZC were identified as stable based on the AMMI model and bi-plot. V11, V5, V21 for grain yield per plant (GYP) and for GZC, V14, and V10 are found to be stable and common in all AMMI stability parameters. V6, V13 and V5 for GYP and V10, V8 and V2 for GZC were identified as stable based on the mean vs. WAASB bi-plot. V21 for GYP and V4 for GZC was the highest yielder and widely adaptable based on WAASBY scores. V13 for GYP and V1 for GZC were all-time winners. V13 and V1 have the highest predicted mean for GYP and GZC, respectively, based on BLUP. V6, V21and V13 were identified as stable and selected based on the multi-trait stability index (MTSI). These selected genotypes selected through BLUP-based stability methods, MTSI, and strength and weakness plots make it easier to evaluate and select genotypes for varietal recommendations and future Zn-fortified rice breeding studies. Keywords: GEI, High Zinc Rice, MTSI, Stable genotype","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarah A. Altwaim, Azzah Alharbi, Shaymaa A. Abdalal, I. Alsaady, Maimonah Alghanmi
{"title":"Prevalence of intestinal parasites in leafy green vegetables consumed by inhabitants of Jeddah city, Saudi Arabia","authors":"Sarah A. Altwaim, Azzah Alharbi, Shaymaa A. Abdalal, I. Alsaady, Maimonah Alghanmi","doi":"10.9755/ejfa.2023.3189","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3189","url":null,"abstract":"Vegetables are a crucial component of every diet and a good source of vitamins and minerals. Many raw vegetables, such as salads, are consumed, and consuming raw vegetables contaminated with human and animal feces can lead to a variety of intestinal parasite diseases. The goal of this cross-sectional study was to investigate the incidence of contamination of green leafy vegetables with human intestinal parasites, as well as identify the most common parasite species and any seasonal variation in parasite abundance in green leafy vegetables found and sold for human consumption in various markets and stores in Jeddah. To the best of our knowledge, no research has been conducted on the prevalence of parasite-contaminated vegetables in Jeddah. From September 2020 to July 2021, 250 leafy vegetables (coriander, watercress, lettuce, parsley, leek, and green onion) were collected from various markets and stores in Jeddah. The vegetables were sorted, labelled, and evaluated in the laboratory after being completely washed with distilled water, examined for sediment, staining, and microscopically examined. Chromatographic immunoassay tests were also used to confirm parasite identification. Intestinal parasites were discovered in 35.2% of the vegetables (88/250). Coriander was the most contaminated (64.2%), while green onion was the least contaminated (21.9%). Blastocystis spp. was the most often discovered parasite (55.7%), followed by Strongyloides spp. larva (22.7%) and Entamoeba coli cysts (6.8%). Summer has the greatest percentage of parasites, followed by autumn, while winter has the fewest. There was no statistically significant relationship between the kind of vegetable, seasonal parasite prevalence in green vegetables, and parasite type.These findings highlight the importance of inspecting vegetables grown or imported from endemic countries for parasite contamination. In Jeddah, several fresh green vegetables were tainted with intestinal parasites. This demonstrates that those who consume raw vegetables regularly may be susceptible to parasitic illnesses. These findings highlight the need of monitoring vegetables grown or imported from parasitic-endemic areas. As a result, it is critical to establish strategies and control measures to limit the likelihood of parasites in food-borne diseases. Keywords: contamination; intestinal parasites; Jeddah; leafy vegetables","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chih-Chiang Wang, Chun Chen, Chih-Wei Chang, Po-Yen Chiu, Fu-An Chen
{"title":"Uric acid–reducing efficacy of Plectranthus amboinicus (Lour.) Spreng in mice with potassium oxonate-induced hyperuricemia","authors":"Chih-Chiang Wang, Chun Chen, Chih-Wei Chang, Po-Yen Chiu, Fu-An Chen","doi":"10.9755/ejfa.2023.3188","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3188","url":null,"abstract":"Plectranthus amboinicus (Lour.) Spreng is a type of Lamiaceae perennial herb that is widely used in folk treatments for respiratory and skin diseases. Notably, research on the hypouricemic effect of P. amboinicus is limited. This study investigated the uric acid–reducing efficacy of P. amboinicus in mice with potassium oxonate-induced hyperuricemia by performing an in vitro xanthine-oxidase-inhibition-activity-guided test of P. amboinicus extract (PAE) and its fractions. The ethyl acetate fraction (EAF) of PAE exhibited optimal efficacy in terms of the phenolic content, DPPH scavenging activity, and xanthine oxidase inhibition. We further demonstrated the uric acid–reducing activity of EAF in a mouse model of potassium bromate–induced hyperuricemia. The results can serve as a useful preclinical reference for researching the gout prevention effects of functional foods. Keywords: Plectranthus amboinicus (Lour.) Spreng, hyperuricemia, uric acid–reducing activity","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139181901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Members’ willingness to pay for sustainability agricultural organization: A Heckman model approach","authors":"Bakiye Kılıç Topuz","doi":"10.9755/ejfa.2023.3178","DOIUrl":"https://doi.org/10.9755/ejfa.2023.3178","url":null,"abstract":"The purpose of this research analyzed the factors influencing the members’ willingness to pay capital in the agricultural cooperatives and unions. The research was performed on a sample 155 members of agricultural organizations in Igdir province of Türkiye using the Simple Random Sampling method. Members’ willingness to pay capital to their organizations were examined using the Contingent Valuation Method and effected factors were analyzed by Heckman Sample Selection model. The research showed that 56.6% of members were willing to pay capital an average of $131 for sustainability of organizations. According to the model results showed that members’ gender, agricultural income, know definition of organization, increase in income of members, pay entry capital, frequency of visiting, meeting the general assembly, cohesion of members positively affected, however, age, household, animal units, negative experience in the past with their organizations negatively affected the members’ willingness to pay capital. In order to sustainable of agricultural organizations, cohesion of members and participation to general assembly must increase. And, encouraging more young and female farmers to become a member, taking an active role cooperatives and unions in marketing and supply input could increase the members’ willingness to pay capital. In addition, training programs should be organized by agricultural organizations and government about increasing capital and economic participation. Keywords: Agricultural organizations; Contingent analysis; Cooperative; Heckman sample selection model; Willingness to pay capital","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139181934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}