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Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB 黑孜然抗结核分枝杆菌H37RV和耐多药结核
Elkawnie Pub Date : 2021-06-30 DOI: 10.22373/ekw.v7i1.9335
M. Masri, Cut Muthiadin, M. Masita, T. Cahyanto, L. Lianah, R. Rusny, S. Tridesianti
{"title":"Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB","authors":"M. Masri, Cut Muthiadin, M. Masita, T. Cahyanto, L. Lianah, R. Rusny, S. Tridesianti","doi":"10.22373/ekw.v7i1.9335","DOIUrl":"https://doi.org/10.22373/ekw.v7i1.9335","url":null,"abstract":"Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79724780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Characterization of Organic Polymer Monolith Columns Containing Ammonium Quarternary As Initial Study For Capillary Chromatography 含季铵盐有机聚合物整体柱的表征及其毛细管色谱的初步研究
Elkawnie Pub Date : 2021-06-30 DOI: 10.22373/ekw.v7i1.8764
A. Rahayu, S. Jamilatun, J. A. Fajri, L. Lim
{"title":"Characterization of Organic Polymer Monolith Columns Containing Ammonium Quarternary As Initial Study For Capillary Chromatography","authors":"A. Rahayu, S. Jamilatun, J. A. Fajri, L. Lim","doi":"10.22373/ekw.v7i1.8764","DOIUrl":"https://doi.org/10.22373/ekw.v7i1.8764","url":null,"abstract":"Abstract: The polymerization process with a simple step has become the centre of attention of several researchers. Various polymers have been developed, although in general, they use polymerization with a post-modification method. A quaternary ammonium monolith organic polymer has been prepared using a simple single thermal method in this research. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride was as the monomer, and ethylene dimethacrylate was as crosslinker. The polymerization proceeded in fused-silica capillary (100 mm, 0.32 mm i.d. x 0.45 mm o.d.) using a one-pot approach method. To achieve the perfect macropores, isopropyl alcohol, PEG 400, and ethanol were used as porogen. Characterization of the surface morphology was carried out using a Scanning Electron Microscope (SEM), and the existence of an amine group was characterized by Fourier Transform Infrared Spectroscopy (FTIR). The distribution size of pores in the polymer was in the range of 1.29 to 3.33 µm.Abstrak: Polimerisasi dengan proses yang sederhana dan simpel menjadi pusat perhatian beberapa peneliti. Berbagai macam polimer telah dikembangkan, akan tetapi pada umumnya menggunakan polimerisasi dengan metode post-modification. Pada penelitian ini, polimer organik yang mengandung amonium kuartener dalam bentuk monolit dengan polimerisasi yang menggunakan suhu tunggal dan sederhana telah dilakukan. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride digunakan sebagai monomer dan ethylene dimethacrylate sebagai crosslinker. Polimerisasi dilakukan dengan metode one-pot aaproach di dalam kapiler silika (100 mm, 0,32 mm i.d. x 0,45 mm o.d.). Untuk mendapatkan makropori yang sempurna, isopropil alkohol, PEG 400 dan etanol digunakan sebagai porogen. Karakterisasi morfologi permukaan dilakukan dengan menggunakan Scanning Electron Microscope (SEM), dan Fourier Transform Infrared Spectroscopy (FTIR) untuk mengidentifikasi gugus fungsi gugus amina yang terdapat pada polimer. Ukuran distribusi pori pada polimer berkisar antara 1,29 sampai 3,33 µm.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81332938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Mineralogical, Microstructural and Compressive Strength Characterization of Fly Ash as Materials in Geopolymer Cement 粉煤灰作为地聚合物水泥材料的矿物学、微观结构和抗压强度表征
Elkawnie Pub Date : 2021-01-17 DOI: 10.22373/EKW.V7I1.7787
C. Rahmawati, S. Aprilia, T. Saidi, T. B. Aulia
{"title":"Mineralogical, Microstructural and Compressive Strength Characterization of Fly Ash as Materials in Geopolymer Cement","authors":"C. Rahmawati, S. Aprilia, T. Saidi, T. B. Aulia","doi":"10.22373/EKW.V7I1.7787","DOIUrl":"https://doi.org/10.22373/EKW.V7I1.7787","url":null,"abstract":"Abstract: This study was designed to examine the mineral, microstructural, and mechanical strength properties of fly ash and its feasibility as a raw material for geopolymer cement. The study used an experimental method by examining the characteristics of fly ash by X-ray Fluorescence Spectrometer (XRF), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), hydrometer method, Scanning electron microscopy (SEM), and compressive strength testing. For creating the geopolymer cement paste, a concentration of NaOH 10M was used, with a ratio of water/solid = 0.4 and a ratio of Na2SiO3/NaOH = 1 using curring at room temperature. The results showed the geopolymer pastes have a compressive strength of 18.1 MPa and 21.5 MPa after 7 days and 28 days. The XRD results showed a decrease in the peak of 2θ at 26.54° because the amorphous part had transformed into a C-S-H solution in geopolymer cement. This finding was supported by the FTIR spectra results showing Si-O-Si bending vibration and the functional group of AlO2. It showed that Nagan Raya fly ash-based geopolymer is a potential construction material.Abstrak: Penelitian ini dirancang untuk mendapatkan sifat mineral, mikrostruktural, dan kekuatan mekanis dari fly ash serta kesesuaiannya sebagai material dasar pada semen geopolimer. Metode penelitian yang digunakan adalah metode eksperimen dengan cara  menguji karakteristik dari fly ash dengan pengujian X-ray Fluorescense Spectrometer (XRF), Fourier transform infrared (FTIR) spectoscopy, X-ray diffraction (XRD), hydrometer method, Scanning electron microscopy (SEM) dan kuat tekan.  Untuk pembuatan pasta semen geopolimer digunakan konsentrasi NaOH 10 M, rasio water/solid 0,4 dan rasio Na2SiO3/NaOH = 1 dengan perawatan pada suhu kamar. Hasil menunjukkan setelah 7 hari pasta geopolimer memiliki kuat tekan 18,1 MPa dan 21,5 MPa pada 28 hari. Hasil XRD menunjukkan adanya penurunan puncak 2θ pada 26,54° ini disebabkan karena bagian amorf dari fly ash telah menjadi larutan C-S-H pada semen geopolimer. Hasil ini diperkuat dengan analisis FTIR spectra yang menunjukkan adanya Si-O-Si bending vibration dan gugus fungsi dari AlO2. Hasil menunjukkan fly ash dari Nagan Raya potensial sebagai bahan material konstruksi berbasis geopolimer.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80599970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods) 鸡粪饲养罗非鱼(Oreochromis niloticus)的研究
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7770
O. T. Kurahman, A. Yuliawati, Lusi Haerunnisa, A. Supriyatna, T. Cahyanto, Y. Suryani, A. Supriadin, C. Hidayat, M. Masri
{"title":"The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)","authors":"O. T. Kurahman, A. Yuliawati, Lusi Haerunnisa, A. Supriyatna, T. Cahyanto, Y. Suryani, A. Supriadin, C. Hidayat, M. Masri","doi":"10.22373/ekw.v6i2.7770","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.7770","url":null,"abstract":"Abstract: In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78931140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh 亚齐叶中酚类化合物的分离及抗氧化活性研究
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.5532
M. Mastura, T. Barus, L. Marpaung, P. Simanjuntak
{"title":"Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh","authors":"M. Mastura, T. Barus, L. Marpaung, P. Simanjuntak","doi":"10.22373/ekw.v6i2.5532","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.5532","url":null,"abstract":"Abstract: An isolation and identification of phenolic compounds from Halban Leaves (Vitex pinnata Linn), a traditional medicinal plant in Aceh, has been done. Halban leaves were extracted through methanol maceration followed by isolation. The methanol extract went into n-hexane and ethyl acetate partition consecutively resulting in 3 different fractions of ethyl acetate and n-hexane extract.  The ethyl acetate fraction demonstrated the most promising antioxidant and cytotoxic activity. Therefore, purification of ethyl acetate fraction was done by column chromatography [SiO2; (i) n-hexane-ethyl acetate 10:1, 1:1 ; (ii) n-hexane-ethyl acetate = 2 : 1); (iii) n-hexane-acetone = 1:1]. The pure isolate as identified by using UV, IR, NMR, and MS spectra afforded 2 phenolic compounds, namely (1) 4-hydroxymethyl benzoate and (2) p-hydroxymethyl benzoic acid (PHBA). The antioxidant activity of compound 1 and cytotoxicity activity of 2 expressed in IC50  and LD50 was 41.08 ppm and 59.41 ppm, respectively.Abstrak: Isolasi dan identifikasi senyawa fenolik dari Daun Halban (Vite xpinnata Linn) tanaman obat tradisional asal Aceh telah dilakukan. Isolasi ini dilakukan dengan cara mengekstraksi daun halban dengan cara maserasi menggunakan pelarut metanol, Ekstrak metanol kemudian dipartiisi dengan n-heksan dan etilasetat secara berturut-turut  sehingga diperoleh  tiga  ekstrak yaitu ekstrak etil asetat, n-heksan dan air. Ekstrak etil asetat memiliki bioaktivitas sebagai antioksidan dan juga toksisitas. Pemurnian dilanjutkan dengan  cara kromatografi kolom [SiO2; (i) n-heksan-etilasetat   10:1,  1:1 ; (ii) n-heksan-etilasetat = 2 : 1); (iii) n-heksan-aseton = 1:1] dan diperoleh isolat  murni. Isolat murni tersebut diidentifikasi berdasarkan interpretasi data spektra UV, IR, NMR dan MS. Hasil interpretasi data menunjukkan daun halban (V. pinnata Linn) mengandung dua senyawa fenolik yaitu (1) 4-hidroksi metil benzoat  dan (2) asam para hidroksi benzoat (PHBA). Senyawa 1  memiliki aktivitas antioksidan dengan nilai IC50 = 41,08bpj dan senyawa 2 memiliki aktivitas toksisitas dengan nilai LC50 = 59,41.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84857240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens 植物病原菌土壤几丁质分解放线菌的分离与筛选
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7400
R. H. Wibowo, S. Sipriyadi, N. R. Mubarik, Iman Rusmana, M. Suhartono
{"title":"Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens","authors":"R. H. Wibowo, S. Sipriyadi, N. R. Mubarik, Iman Rusmana, M. Suhartono","doi":"10.22373/ekw.v6i2.7400","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.7400","url":null,"abstract":"Abstract: Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on  Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan  mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85867470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach 昆虫病原真菌分离物的分子鉴定及系统发育分析
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6549
S. Syaukani, Zulia Ananda, S. Suhartono, Sirtina Sirtina, Oviana Lisa, A. Alfizar, S. Samingan
{"title":"Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach","authors":"S. Syaukani, Zulia Ananda, S. Suhartono, Sirtina Sirtina, Oviana Lisa, A. Alfizar, S. Samingan","doi":"10.22373/ekw.v6i2.6549","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.6549","url":null,"abstract":"Abstract: Entomopathogenic fungi are essential to consider as one of the biological agents to control termite populations. This research aimed to molecularly identify entomopathogenic fungi isolates in termites based on the ITS rDNA region and to determine the relationship of fungi isolates. Identification was performed by DNA extraction, PCR amplification, electrophoresis, purification, and sequencing. Phylogenetic trees were generated using MEGA X.  Molecular identification showed that the ISO1 sample was Penicillium oxalicum, the ISO2 sample was Trichoderma ghanense the ISO3 sample was Aspergillus niger, the ISO4 sample was Aspergillus fumigatus and the ISO5 sample was Aspergillus pseudonomius. The phylogenetic tree showed that the ISO1, ISO2, ISO3, ISO4, and ISO5 samples had the closest relationship with Penicillium oxalicum strain FR6-CGR12, Trichoderma ghanense isolate TM2, Aspergillus niger isolate 77, Aspergillus fumigatus, and Aspergillus pseudonomius strain DTO 267D6, respectively.Abstrak: Kelimpahan jenis fungi entomopatogen adalah hal yang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap.Penelitian ini bertujuan untuk mengidentifikasi secara molekular isolat fungi entomopatogen pada rayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut. Identifikasi dilakukan dengan cara ekstraksi DNA, amplifikasi menggunakan PCR, elektroforesis, purifikasi dan sekuensing. Selanjutnya kontruksi pohon filogenetik menggunakan aplikasi MEGA X. Berdasarkan uji molekular menunjukkan bahwa sampel ISO1 merupakan Penicillium oxalicum.ISO2 merupakan Trichoderma ghanense.ISO3 merupakan Aspergillus niger. ISO4 merupakan Aspergillus fumigatus.ISO5 merupakan Aspergillus pseudonomius.Konstruksi pohon filogenetik menunjukkan bahwa, sampel ISO1 berkerabat dekat dengan Penicillium oxalicum strain FR6-CGR12. Sampel ISO2 berkerabat dekat dengan Trichoderma ghanense isolat TM2. Sampel ISO3 berkerabat dekat dengan Aspergillus nigerisolat 77. Sampel ISO4 berkerabat dekat dengan Aspergillus fumigatus.Sampel ISO5 berkerabat dekat dengan Aspergillus pseudonomius strain DTO 267D6.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74885529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hotel Building Reliability Towards Building Age 面向建筑时代的酒店建筑可靠性
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6313
Syukri Indrawansyah, Abdullah Abdullah, C. Z. Oktaviani
{"title":"Hotel Building Reliability Towards Building Age","authors":"Syukri Indrawansyah, Abdullah Abdullah, C. Z. Oktaviani","doi":"10.22373/ekw.v6i2.6313","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.6313","url":null,"abstract":"Abstract: Mahara Hotel is a tourism-supporting facility and infrastructure first established in Central Aceh District in 1999. This 3-story hotel used a reinforced concrete frame structure, and as time goes by, the service capacity of the building would decrease. This condition was suspected to affect quality and user convenience. Anticipate the older age of the building, and better maintenance management is applied so that the proper building’s function can be maintained. The purpose of this study was to assess the reliability of 20 year-established hotel buildings. The method was a descriptive analysis. Data were analyzed based on the guidelines of Technical Procedures for Guidelines for Certificate of Eligibility for Functions (SLF) of Buildings Regulation of  Minister of Public Works No. 25/PRT/M/2007. Based on the results, the reliability value of the Mahara Hotel building was unreliable (66.75 %), less than the SLF guidelines (at least 75 %). Some components that caused a low level of reliability to include utility and fire protection components (20.34 %) and accessibility components (3.11 %). The effective and efficient component in increasing the reliability of the building was the utility and fire protection components by not requiring major demolition.Abstrak: Hotel Mahara merupakan sarana dan prasarana penunjang pariwisata yang pertama kali berdiri di Kabupaten Aceh Tengah pada tahun 1999. Hotel berlantai 3 ini menggunakan struktur rangka beton bertulang dan seiring dengan berjalannya waktu, daya layan gedung akan semakin menurun. Kondisi ini ditenggarai akan mempengaruhi kualitas dan kenyamanan pengguna. Untuk mengantisipasi hal tersebut, maka semakin tua umur bangunan, seharusnya didukung dengan penerapan manajemen pemeliharaan yang baik agar kelayakan fungsi dari bangunan dapat dipertahankan. Tujuan dari penelitian ini untuk menilai keandalan bangunan hotel setelah berumur 20 tahun. Metode yang digunakan adalah analisis deskriptif. Data dianalisis berdasarkan panduan Teknis Tata Cara Pedoman Sertifikat Laik Fungsi (SLF) Bangunan Gedung Peraturan Menteri Pekerjaan Umum No. 25/PRT/M/2007. Berdasarkan hasil penelitian, nilai keandalan bangunan gedung Hotel Mahara termasuk tidak andal yaitu sebesar 66.75 %, kurang dari yang dipersyaratkan oleh pedoman SLF tersebut yaitu minimal 75 %. Beberapa komponen yang menyebabkan rendahnya tingkat keandalan anatara lain komponen utilitas dan proteksi kebakaran sebesar 20.34 % serta komponen aksesibilitas sebesar 3.11 %. Komponen yang efektif serta efisien dalam menaikkan tingkat keandalan bangunan gedung tersebut adalah komponen utilitas dan proteksi kebakaran dengan tidak mengharuskan pembongkaran yang besar.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79425762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Prevention of Ulcers Decubitus with Mobilization and The Usage of Olive Oil on Stroke Patients 运动预防脑卒中患者褥疮及橄榄油的应用
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6925
Siti Cahaya Meliza, Kiking Ritarwa, Nurdin Sitohang
{"title":"The Prevention of Ulcers Decubitus with Mobilization and The Usage of Olive Oil on Stroke Patients","authors":"Siti Cahaya Meliza, Kiking Ritarwa, Nurdin Sitohang","doi":"10.22373/ekw.v6i2.6925","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.6925","url":null,"abstract":"Abstract: Decubitus ulcers are one of the problems experienced by bed rest patients, such as stroke patients, caused by immobilization. Prevention of decubitus ulcers can be done with mobilization and topical application of olive oil. Olive oil contains saturated fatty acids, unsaturated fatty acids, vitamin E, and phenols which are healthy for the skin. This research aims to identify the effects of mobilization and application of olive oil on the prevention of decubitus ulcers in stroke patients. A control group pretest-posttest quasi-experimental design was used in the research. Convenience (non-probability) sampling was also carried out, resulting in 64 research samples. Mobilization intervention of right-left oblique positions and application of olive oil on the area prone to decubitus ulcers were then executed for seven days. Data analysis using the Wilcoxon signed-rank test showed the effects of mobilization and application of olive oil on the preventions of decubitus ulcers in stroke patients with a value of P= <0,05. Abstrak: Ulkus dekubitus merupakan salah satu masalah yang dialami pasien tirah baring seperti pasien stroke, dengan faktor penyebabnya adalah immobilisasi, tindakan pencegahan ulkus dekubitus dapat dilkukan dengan mobilisasi dan pemberian bahan oles seperti minyak zaitun, minyak zaitun kandungannya terdiri dari asam lemak jenuh, asam lemak tak jenuh, vitamin E dan fenolik yang baik untuk kesehatan kulit. Penelitian ini bertujuan untuk mengidentifikasi pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke. Penelitian menggunakan desain Quasi-experimental dengan pretest- posttest with countrol gruopdesign.Convenience (Non-probability) dilakukan untuk pengambilan sampel, jumlah sampel penelitian 64. Intervensi mobilisasi miring kanan dan kiri kemudian bagian yang akan terjadi ulkus dekubitus diolesi minyak zaitun,intervensi dilakukan selama tujuh hari. Analisa data wilcoson signed -rank test menunjukan adanya pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke dangan nilai P= <0,05. Mobilisasi miring kanan dan kiri penggunaan minyak zaitun dapat mencegah ulkus dekubitus.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76920816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Knowledge Transfer About Earthquake Disaster Mitigation To Children Through TF-IDF 通过TF-IDF向儿童传递地震减灾知识
Elkawnie Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7281
Maria Umran, H. M. Sarim
{"title":"Knowledge Transfer About Earthquake Disaster Mitigation To Children Through TF-IDF","authors":"Maria Umran, H. M. Sarim","doi":"10.22373/ekw.v6i2.7281","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.7281","url":null,"abstract":"Abstract: Past observations during a disaster identify that when children are separated from parents, they suffer due to the inability to comprehend disaster mitigation concepts. This study proposes a process from the existing framework K-Nearest Neighbor (KNN) and Term Frequency - Inverse Document Frequency (TF-IDF) for extracting a large body of knowledge in the form of documents into simple words. Those simple words can be arranged into contextual lyrics utilizing an Artificial Intelligence lyrics generator and then orchestrated into a song using a music generator. The piece, which is the output of the proposed process, is utilized to transfer the knowledge about earthquake disaster mitigation to children. A quantitative analysis of questionnaires on students aged 9-10 in Banda Aceh shows the song's highly significant effect in transferring the knowledge about earthquake disaster mitigation to children.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79594001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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