Eicosanoids最新文献_第4页

Characterization of an inositol 1,3,4,5-tetrakisphosphate 3-phosphatase from porcine brain. 猪脑肌醇1,3,4,5-四聚磷酸3-磷酸酶的研究。

Eicosanoids Pub Date : 1992-01-01

A Höer, E Oberdisse

引用次数: 0

Mouse peritoneal macrophages contain an acylating system specific for twenty-carbon polyunsaturated fatty acids. A study with intact cells. 小鼠腹膜巨噬细胞含有一个针对20碳多不饱和脂肪酸的酰化系统。完整细胞的研究。

Eicosanoids Pub Date : 1992-01-01

B Fernández, J A Solís-Herruzo, J Balsinde

{"title":"Mouse peritoneal macrophages contain an acylating system specific for twenty-carbon polyunsaturated fatty acids. A study with intact cells.","authors":"B Fernández, J A Solís-Herruzo, J Balsinde","doi":"","DOIUrl":"","url":null,"abstract":"It is widely recognized that in addition to regulating the expression and activity of arachidonic acid (AA)-metabolizing enzymes, the availability of free AA limits eicosanoid biosynthesis. AA participates in a deacylation/reacylation cycle of membrane phospholipids in which the fatty acid is cleaved by phospholipase A2 and reesterified by acyltransferase. Thus, free AA can become available either by phospholipase A2 activation or by inhibition of fatty acid reincorporation. We observed that exposure of [3H]AA-prelabeled macrophages to micromolar concentrations of unlabeled AA resulted in a net release of 3H-radioactivity into the extracellular medium. This was not the consequence of phospholipase A2 activation, but of impaired reesterification of previously liberated [3H]AA. The eicosanoid precursors eicosatrienoic acid (ETA) and eicosapentaenoic acid (EPA) mimicked the effect of unlabeled AA on 3H-radioactivity release from [3H]AA-prelabeled macrophages, but all other fatty acids tested were ineffective. Similarly, only AA, ETA and EPA were able to inhibit [3H]AA uptake by macrophages, all other fatty acids being ineffective. From these data, it is concluded that intact macrophages contain an acylating system specific for the eicosanoid precursors AA, ETA and EPA. Altogether, the results of this study underscore the importance of fatty acid reacylation in controlling free AA levels in macrophages.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 2","pages":"115-20"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12620460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of calcimycin-induced stimulation of cytosolic calcium in human PMN by a LTB4 receptor antagonist. LTB4受体拮抗剂抑制钙霉素诱导的人PMN胞质钙的刺激。

Eicosanoids Pub Date : 1992-01-01

R Heckenberger, K Schrör

引用次数: 0

Effect of 25-hydroxycholesterol on cytotoxicity and prostacyclin production in cultured human umbilical arterial endothelial cells. 25-羟基胆固醇对培养的人脐动脉内皮细胞毒性和前列环素生成的影响。

Eicosanoids Pub Date : 1992-01-01

K Kawamura, F A Kummerow

引用次数: 0

Thromboxane as diagnostic tool and therapeutic target in cardiovascular disease. 血栓素作为心血管疾病的诊断工具和治疗靶点。

Eicosanoids Pub Date : 1992-01-01

R Lorenz, P C Weber

引用次数: 0

Sulotroban selectively inhibits thromboxane-receptor-mediated responses in the peripheral vascular bed of the cat. 舒洛曲班选择性抑制猫周围血管床血栓素受体介导的反应。

Eicosanoids Pub Date : 1992-01-01

P Kvamme, R K Minkes, P J Kadowitz

{"title":"Sulotroban selectively inhibits thromboxane-receptor-mediated responses in the peripheral vascular bed of the cat.","authors":"P Kvamme, R K Minkes, P J Kadowitz","doi":"","DOIUrl":"","url":null,"abstract":"The effects of Sulotroban (BM 13.177, SK&F 95587) were investigated under conditions of controlled blood flow in the hindquarters and mesenteric vascular beds of the cat. Injections of the thromboxane (TX) A2 mimics, U46619 and U44069, caused dose-related increases in perfusion pressure. After administration of SK&F 95587, vasoconstrictor responses to the TXA2 mimics were reduced significantly, and the dose-response curves were shifted to the right in a parallel fashion. Responses to norepinephrine, phenylephrine, tyramine, endothelin-1, angiotensin II, BAY K8644, acetylcholine, sodium nitroprusside, isoproterenol, lemakalim, prostaglandin (PG) E1, and PGF2 alpha, agents which alter vascular resistance by a variety of mechanisms, were not changed by the TXA2 receptor antagonist. However, SK&F 95587 reduced mesenteric vasoconstrictor responses to PGD2. Results of the present study indicate that SK&F 95587 blocks TX-receptor-mediated responses in the hindquarters circulation of the cat in a competitive and selective manner and reduces mesenteric vascular responses to the TXA2 mimics, as well as PGD2. These data suggest that this antagonist would be useful in studies on the role of TXA2 in physiologic and pathophysiologic processes in the systemic vascular bed of the cat.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 3-4","pages":"127-33"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12466825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epicardial versus endocardial "in mirror" changes in prostaglandin synthesis after short periods of ischemia and reperfusion. 短时间缺血再灌注后前列腺素合成的心外膜与心内膜“镜中”变化。

Eicosanoids Pub Date : 1992-01-01

B Rabinowitz, M Arad, E Elazar, R Klein, Y Har Zahav

{"title":"Epicardial versus endocardial \"in mirror\" changes in prostaglandin synthesis after short periods of ischemia and reperfusion.","authors":"B Rabinowitz, M Arad, E Elazar, R Klein, Y Har Zahav","doi":"","DOIUrl":"","url":null,"abstract":"Cardiac ischemia and reperfusion are associated with increased prostaglandin levels in the coronary venous effluent. This study implemented an in vivo-in vitro technique to investigate regional alterations in heart tissue PGE2 and PGF2 alpha de novo production. Canine endocardial and epicardial explants were incubated following 5 min regional ischemia, or 5 min ischemia with 10 min reperfusion, induced in vivo in two groups of animals (n = 10 and 6, respectively). Ischemia produced a significant upsurge in endocardial but not in epicardial prostaglandin synthesis as compared with the non-ischemic zone: 7.6 +/- 0.7 versus 4.5 +/- 0.5 pg/mg tissue per h in PGE2 (P < 0.001) and 8.8 +/- 1.2 versus 6.8 +/- 1.2 pg/mg per h PGF2 alpha (P < 0.01). Following reperfusion, PGE2 was higher in the apparently normal than in the affected endocardium (5.8 +/- 0.6 versus 4.4 +/- 0.5 pg/mg per h, P < 0.05). Opposite changes occurred in the reperfused epicardium: 8.2 + 0.9 versus 4.9 +/- 0.7 pg/mg per h PGE2 (P < 0.01) and 11.1 +/- 0.9 versus 6.0 +/- 0.6 pg/mg per h PGF2 alpha (P < 0.001), for the reperfused as compared to the \"normal\" region, respectively. Our findings imply that the left ventricular wall is not homogeneous in its eicosanoid response to ischemia and reperfusion. \"In mirror\" changes were found between endocardium end epicardium and between the injured and the apparently normal regions.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 3-4","pages":"163-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12467465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of viral infection on eicosanoid formation and procoagulant activity of rat peritoneal macrophages. Role of the dietary fat type. 病毒感染对大鼠腹腔巨噬细胞类二十烷形成及促凝活性的影响。膳食脂肪类型的作用。

Eicosanoids Pub Date : 1992-01-01

W Engels, P J Lemmens, A D Muller, M C Van Dam-Mieras, G Hornstra

{"title":"The effect of viral infection on eicosanoid formation and procoagulant activity of rat peritoneal macrophages. Role of the dietary fat type.","authors":"W Engels, P J Lemmens, A D Muller, M C Van Dam-Mieras, G Hornstra","doi":"","DOIUrl":"","url":null,"abstract":"The effect of dietary manipulation on eicosanoid formation in rat peritoneal macrophages was studied in relation to some of their effector functions: cellular procoagulant activity, production of reactive oxygen species (measured as chemiluminescence), and phagocytosis of antibody-coated erythrocytes. Rats were fed adequate diets for eight weeks containing mackerel oil (MO), sunflowerseed oil (SO) or hydrogenated coconut oil (HCO). The release of eicosanoids from resident macrophages stimulated by opsonized zymosan was significantly lower for the MO group as compared to the other dietary groups. Infection of the animals via intraperitoneal injection with rat cytomegalovirus resulted in a significant decrease in eicosanoid production in all groups, irrespective of dietary fat type. However, in the HCO group a partial restoration of TXB2 and HHT production could be observed at day 10 post infection. Resident macrophages obtained from the mackerel oil fed animals showed a significantly higher procoagulant activity than those from the other diet groups. Infection of the animals resulted in an increase in procoagulant activity in all groups. In contrast, no significant differences in chemiluminescence and in phagocytosis were detected between macrophages obtained from rats fed the different diet groups. It is concluded that peritoneal macrophages obtained from mackerel oil fed rats produce less eicosanoids and are more procoagulant than those from the other dietary groups, but a viral infection eradicates these differences. Therefore, a correlation between eicosanoid formation and effector functions studied could not be established.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 2","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12507501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostaglandin 9-ketoreductase from pig and human kidney: purification, properties and identity with human carbonyl reductase. 猪和人肾中前列腺素9-酮还原酶的纯化、性质及其与人羰基还原酶的特性。

Eicosanoids Pub Date : 1992-01-01

A Schieber, S Ghisla

引用次数: 0

Priming mechanisms and induction of heat shock proteins in human polymorphonuclear granulocytes induced by eicosanoids and cytokines. 类二十烷和细胞因子诱导人多形核粒细胞热休克蛋白的启动机制和诱导。

Eicosanoids Pub Date : 1992-01-01

W König, M Köller, J Brom

引用次数: 0