Eicosanoids最新文献_第3页

The prostacyclin analogues, cicaprost and iloprost, increase cytosolic Ca2+ concentration in the human erythroleukemia cell line, HEL, via pertussis toxin-insensitive G-proteins. 前列环素类似物环卡前列素和伊洛前列素通过百日咳毒素不敏感的g蛋白增加人红白血病细胞株HEL的胞浆Ca2+浓度。

Eicosanoids Pub Date : 1992-01-01

I Schwaner, R Seifert, G Schultz

{"title":"The prostacyclin analogues, cicaprost and iloprost, increase cytosolic Ca2+ concentration in the human erythroleukemia cell line, HEL, via pertussis toxin-insensitive G-proteins.","authors":"I Schwaner, R Seifert, G Schultz","doi":"","DOIUrl":"","url":null,"abstract":"In the human erythroleukemia cell line, HEL, prostaglandin E2 (PGE2) and the stable prostacyclin analogue, iloprost, increase cytosolic Ca2+ concentration ([Ca2+]i) via pertussis toxin-sensitive and -insensitive pathways. Unlike iloprost, the stable prostacyclin analogue cicaprost (ZK 96480), is devoid of agonistic properties at prostaglandin E2 receptors. We compared the effects of cicaprost, iloprost and PGE2 on [Ca2+]i in HEL cells. Cicaprost, iloprost and PGE2 were similarly potent to increase [Ca2+]i in HEL cells. However, unlike the effects of PGE2, those of the prostacyclin analogues were not inhibited by pertussis toxin. The prostaglandins studied increased [Ca2+]i through both mobilization from internal stores and Ca2+ influx from the extracellular space. Prostacyclin analogue- and PGE2-induced rises in [Ca2+]i were desensitized in a homologous manner. Additionally, there was cross-desensitization between cicaprost and iloprost, but not between the prostacyclin analogues and PGE2. Our data suggest that in HEL cells (i) cicaprost and iloprost act through prostacyclin receptors and (ii) that these receptors couple to pertussis toxin-insensitive heterotrimeric regulatory guanine nucleotide-binding proteins, (iii) resulting in an increase in [Ca2+]i by Ca2+ mobilization from internal stores and sustained influx.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 Suppl ","pages":"S10-2"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12455502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of leukotriene biosynthesis in human polymorphonuclear leukocytes. 人多形核白细胞白三烯生物合成的调控。

Eicosanoids Pub Date : 1992-01-01

M Schatz-Munding, V Ullrich

引用次数: 0

Formation of lipoxins and leukotrienes by human alveolar macrophages incubated with 15(S)-HETE: a model for cellular cooperation between macrophages and airway epithelial cells. 15(S)-HETE培养的人肺泡巨噬细胞形成脂毒素和白三烯:巨噬细胞与气道上皮细胞之间细胞合作的模型。

Eicosanoids Pub Date : 1992-01-01

C Chavis, P Godard, A Crastes de Paulet, M Damon

{"title":"Formation of lipoxins and leukotrienes by human alveolar macrophages incubated with 15(S)-HETE: a model for cellular cooperation between macrophages and airway epithelial cells.","authors":"C Chavis, P Godard, A Crastes de Paulet, M Damon","doi":"","DOIUrl":"","url":null,"abstract":"Human alveolar macrophages (AM) from bronchoalveolar lavage of asthmatic patients (AP) and healthy volunteers (HS) were compared for their respective capacities to produce lipoxins and leukotrienes when stimulated by calcium ionophore A23187 with or without 15(S)-HETE. The metabolites were analyzed using an isocratic RP-HPLC system and their formation profiles evaluated on the basis of chromatographic behaviour, UV spectral characteristics and co-elution with synthetic standards. Without 15-HETE, AM from AP produced more LTB4 and 5-HETE than those from HS. In the presence of 15-HETE, human AM were able to produce 5,15-diHETE and lipoxins. Moreover, the total amount of lipoxins synthesized by AM from AP was 2 fold higher than that synthesized by AM from HS, thus showing an enhanced cell activation via the 5-lipoxygenase (5-LO) pathway. These results presented AM as in vitro 15-HETE metabolizing cells and suggested some hypothesis about human AM 5-LO regulation mechanism. The enhanced 5-LO activity in AM from AP suggested that in vivo they could participate in cell to cell interaction mechanisms involved in inflammatory lung diseases and might also take up and transform 15-HETE predominantly released by airway epithelial cells.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 3-4","pages":"203-11"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of prostaglandin E2 or prostaglandin synthesis inhibitors on human gonadotrophins and prolactin. 前列腺素E2或前列腺素合成抑制剂对人促性腺激素和催乳素的影响。

Eicosanoids Pub Date : 1992-01-01

M Toppozada, F Gerges, H Khalil, S Marzouk, A Kholeif

引用次数: 0

Modulation of arachidonic acid metabolism and cyclic AMP content of human alveolar macrophages. 人肺泡巨噬细胞花生四烯酸代谢和环AMP含量的调节。

Eicosanoids Pub Date : 1992-01-01

M Bachelet, C Chouaid, N Havet, J Masliah, A Barre, B Housset, B B Vargaftig

引用次数: 0

Elevated maternal plasma immunoreactive phospholipase A2 in human preterm and term labour. 孕妇血浆免疫反应性磷脂酶A2在早产儿和足月分娩中的升高。

Eicosanoids Pub Date : 1992-01-01

G E Rice, S P Brennecke, K F Scott, G M Smith, I A Rajkovic, G J Bishop

引用次数: 0

Aspirin abolishes the decreased low-density lipoprotein (LDL) entry into the rabbit arterial wall induced by the calcium channel blocker isradipine. 阿司匹林可消除钙通道阻滞剂伊斯拉地平引起的兔动脉壁低密度脂蛋白(LDL)进入减少。

Eicosanoids Pub Date : 1992-01-01

H Sinzinger, I Virgolini, J O'Grady, A Keiler, G Lupattelli, P Angelberger, E Molinari

{"title":"Aspirin abolishes the decreased low-density lipoprotein (LDL) entry into the rabbit arterial wall induced by the calcium channel blocker isradipine.","authors":"H Sinzinger, I Virgolini, J O'Grady, A Keiler, G Lupattelli, P Angelberger, E Molinari","doi":"","DOIUrl":"","url":null,"abstract":"After deendothelialization and experimentally induced hypercholesterolemia in rabbits, an increased LDL entry into the vascular wall can be monitored using radiolabelled LDL. In male rabbits aged 6 months the abdominal aortic endothelium was removed by a Fogarty catheter. The animals fed a 1% cholesterol supplemented diet were treated either with isradipine (0.3 mg/kg/daily) (n = 36) alone or in combination with aspirin (5 mg/kg/daily) (n = 36) for four weeks. Thirty-six animals served as controls. 1, 3, 6, 12, 24 and 48 hours prior to sacrificing, 10 microCi 125I-LDL was administered intravenously to six rabbits in each group. The LDL entry was quantified in the abdominal aorta according to morphologically assessed type of surface lining. Aortic cholesterol content was assessed by Sudan-III staining and quantitative determination. Endothelialized segments exhibited a significantly (p less than 0.05 - p less than 0.001) lower LDL uptake as compared to re- or deendothelialized segments. The LDL entry was significantly lower with isradipine treatment than in controls. In parallel the cholesterol content decreased and the Sudan-III-positive areas were smaller in size. This beneficial effect as well as that on aortic lipid content was abolished by a pretreatment with aspirin. While in the isradipine-treated animals PGI2 synthesis was significantly (p less than 0.01) enhanced, it was almost completely blocked by aspirin. These findings indicate that the benefit of reduced LDL entry caused by isradipine may be mediated by an increased endogenous PGI2 synthesis.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 1","pages":"13-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12589781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipoxygenase inhibitors but not site specific 5-lipoxygenase blockers protect against endotoxic shock and inhibit production of tumor necrosis factor. 脂氧合酶抑制剂而非位点特异性5-脂氧合酶阻滞剂可防止内毒素休克和抑制肿瘤坏死因子的产生。

Eicosanoids Pub Date : 1992-01-01

F U Schade, R Engel, D Jakobs

引用次数: 0

Epidermal derived growth factor stimulates the prostaglandin F2 alpha-synthesis in rabbit luteal cells. 表皮源性生长因子刺激兔黄体细胞中前列腺素F2 α的合成。

Eicosanoids Pub Date : 1992-01-01

C Chudaska, R Diallo, W Schlegel

引用次数: 0

Membranes exert indirect negative control on phospholipase A2 (PLA2) in human placenta. 人胎盘膜对磷脂酶A2 (PLA2)有间接负调控作用。

Eicosanoids Pub Date : 1992-01-01

J Y Jeremy, M A Barradas, D P Mikhailidis

引用次数: 0