Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences最新文献_第2页

EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT 印楝叶乙醇提取物对兔睾丸精细胞发育及雄激素受体表达的影响

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-04-05 DOI: 10.21157/j.ked.hewan.v17i1.30042

Usma Aulia, S. Wahyuni, G. Gholib, D. Dasrul, M. Adam, E. Rahmi, A. Hamzah

{"title":"EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT","authors":"Usma Aulia, S. Wahyuni, G. Gholib, D. Dasrul, M. Adam, E. Rahmi, A. Hamzah","doi":"10.21157/j.ked.hewan.v17i1.30042","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v17i1.30042","url":null,"abstract":"The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114132683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAPABILITY OF DIFFERENT BREEDS OF DONOR CATTLE TO PRODUCE EMBRYOS AT THE CIPELANG LIVESTOCK EMBRYO CENTER, BOGOR, WEST JAVA 西爪哇茂物cipelang家畜胚胎中心不同品种供体牛的胚胎生产能力

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-04-05 DOI: 10.21157/j.ked.hewan.v17i1.24673

Crista Damaris, Anny Rosmayanti, Siti Darojah, N. Isnaini

{"title":"CAPABILITY OF DIFFERENT BREEDS OF DONOR CATTLE TO PRODUCE EMBRYOS AT THE CIPELANG LIVESTOCK EMBRYO CENTER, BOGOR, WEST JAVA","authors":"Crista Damaris, Anny Rosmayanti, Siti Darojah, N. Isnaini","doi":"10.21157/j.ked.hewan.v17i1.24673","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v17i1.24673","url":null,"abstract":"The objective of this research was to investigate the capability of the donor cattle to produce embryos by comparing three cattle breeds, namely Bos taurus beef cattle (Limousin), Bos taurus dairy cattle (Friesian Holstein/FH), and Bos indicus beef cattle (Peranakan Ongole/PO). This research was observational method with in vivo embryo production method and a nonsurgical embryo collection method (flushing). The data collected were then processed using the one way ANOVA method. The results showed that the number of CL, response rate, total number of embryos, and recovery rate in PO cattle, FH cattle, and Limousin cattle did not differ significantly (P0.05). The appropriate number of embryos to be transferred and degenerative embryo were significantly different among PO cattle, FH cattle, and Limousin cattle (P0.05), while the unfertil ova, fertilization rate, and transferable embryo did not differ among cattle breed (P0.05). This research concluded that each breed of the donor cattle produced embryos with varies quality and the best quality of embryo production is found in Limousin cattle.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124648074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECTIVENESS OF PROBIOTIC AND DIGESTIVE ENZYMES IN FERMENTED DIET ON GROWTH, FAT AND CHOLESTEROL CONTENTS OF BROILER 发酵饲粮中益生菌和消化酶对肉鸡生长、脂肪和胆固醇含量的影响

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-02-14 DOI: 10.21157/j.ked.hewan.v16i4.25146

Dr. M Aman Yaman

{"title":"EFFECTIVENESS OF PROBIOTIC AND DIGESTIVE ENZYMES IN FERMENTED DIET ON GROWTH, FAT AND CHOLESTEROL CONTENTS OF BROILER","authors":"Dr. M Aman Yaman","doi":"10.21157/j.ked.hewan.v16i4.25146","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i4.25146","url":null,"abstract":"ABSTRACTThe present study aims to determine the effect of fermented diet containing probiotics and digestive enzymes with different feed levels on growth, fat and cholesterol contents of broiler chickens. Evaluating the effectiveness of probiotics (P) and digestive enzymes (DE) in fermented diet on broiler was observed by 4 treatments: P0=control (fermented diet without P and ED) EP), P1(fermented diet contained 0.5% P only)), P2 (fermented diet contained 0.5% DE only), P3 (fermented diet contained 0.5% P and 1% DE) and P4 (fermented diet contained 1% P and 0.5% DE). A hundred DOCs of broiler chickens strain CP 707 were used (unsex) and it designed by a completely randomized design with 5 treatments, 4 replications and each replication consisted of 5 DOCs. The data obtained were analyzed by means of variance and Duncan's multiple range test. The results of present study showed that broiler fed on fermented diet containing P and/or ED with different levels had a significant effect (P0.05) on final body weight, weight gain, carcass weight, fat content on thigh muscle. Broiler fed on fermented diet contained 0.5% DE only (P2) resulted in better growth and weight gain than other treatments. However, fermented diet contained probiotics and digestive enzymes had no effect on fat and cholesterol contents in breast muscle. It was concluded that the addition of digestive enzymes in fermented diet formulated by local raw materials was very effective than probiotics in increasing body weight gain, carcass weight and prevented the increase in fat and cholesterol contents both in breast and thight muscles of broiler chicken.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126583030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MORPHOFUNCTIONAL CHARACTERISTICS OF THE LOWER HINDLIMB AND FOOT MUSCLES OF BORNEAN ORANGUTAN (Pongo pygmaeus) 婆罗洲猩猩下后肢和足部肌肉的形态功能特征

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-01-17 DOI: 10.21157/j.ked.hewan.v16i4.28199

Nicolas Edward Christanto Kartjito, Ikhsan Kadarusman, S. Novelina, C. Nisa', Sri Rahmatul Laila, D. Cahyadi, N. Nurhidayat

{"title":"MORPHOFUNCTIONAL CHARACTERISTICS OF THE LOWER HINDLIMB AND FOOT MUSCLES OF BORNEAN ORANGUTAN (Pongo pygmaeus)","authors":"Nicolas Edward Christanto Kartjito, Ikhsan Kadarusman, S. Novelina, C. Nisa', Sri Rahmatul Laila, D. Cahyadi, N. Nurhidayat","doi":"10.21157/j.ked.hewan.v16i4.28199","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i4.28199","url":null,"abstract":"Orangutan is a great ape that has anatomical and physiological aspects similar to those of gorillas and chimpanzees, although orangutan is more arboreal than other great apes. This research aims to study the morphofunctional characteristics of the lower hindlimb and foot muscles of Bornean orangutan and its relation to the type of locomotion and daily behavior. This research was conducted on the right leg of a male orangutan by observing the morphology of the lower hindlimb and foot muscles and observing the behavior of the Bornean orangutan through video observations. The results showed that digital flexor muscles are more developed than plantar flexor muscles as an adaptation towards arboreal activities that require finger flexor movements such as gripping tree branches. Orangutans have rudimentary hallux, which is related by the less developed intrinsic muscle and indicated by the way orangutan grips tree branches dominantly using digits II-V. At a certain time, male Bornean orangutans are active on terrestrial that causes the digital extensor muscles to have more developed as an adaptation to terrestrial activity. It can be concluded the morphology of the lower hindlimb and foot muscles is in accordance to its function and behavior adaptation in arboreal and terrestrial. ","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124804190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFICACY OF AVOCADO, MUNG BEAN SPROUTS, AND HOLY BASIL HERB COMBINATION (JAMU ATOKE) ON THE HEALTH AND REPRODUCTIVITY OF ADULT FEMALE RATS 牛油果、绿豆芽和圣罗勒草药组合(jamu atoke)对成年雌性大鼠健康和生殖能力的影响

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-01-17 DOI: 10.21157/j.ked.hewan.v16i4.24037

Andriyanto Andriyanto, Leliana Nugrahaning Widi, Hamdika Yendri, Kharisma Mardathilah, Diky Yuliansah, F. Agustin, A. Mustika, W. Manalu

{"title":"THE EFFICACY OF AVOCADO, MUNG BEAN SPROUTS, AND HOLY BASIL HERB COMBINATION (JAMU ATOKE) ON THE HEALTH AND REPRODUCTIVITY OF ADULT FEMALE RATS","authors":"Andriyanto Andriyanto, Leliana Nugrahaning Widi, Hamdika Yendri, Kharisma Mardathilah, Diky Yuliansah, F. Agustin, A. Mustika, W. Manalu","doi":"10.21157/j.ked.hewan.v16i4.24037","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i4.24037","url":null,"abstract":"The purpose of this study was to examine the efficacy of a herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing the reproductive health of adult female rats. Eighteen female Sprague Dawley rats aged 9-10 weeks old weighing around 180-250 g, and had not been pregnant previously were equally and randomly divided into 3 groups: one control group and two treatment groups (n= 6 rats per group). The treatment groups received Jamu ATOKE with a concentration of 2.5 and 5%, respectively. Jamu ATOKE was added to the drinking water and consumed by the rats for 30 days prior to pregnancy. Reproductivity (pregnancy success, estrogen and progesterone hormone concentrations), and health performance (body weight gain, food, and water consumption, motor activity, red blood cell (RBC) count, white blood cell (WBC) count and differentials, SGPT, SGOT, ureum, and creatinine) of rats were observed. The results showed that groups receiving the Jamu ATOKE had better reproductive and health performance compared to those of the control group. The administration of Jamu ATOKE can significantly increase the concentration of estrogen and progesterone in rats (P0.05). It can also improve the fertility and health of the female rats prior to pregnancy.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"6 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128257035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HEPARIN AND HYPOTAURINE SUPPLEMENTATION IMPROVE THE FERTILIZATION RATE OF SHEEP OOCYTES MATURED IN MEDIA CONTAINING L-CARNITINE IN VITRO 肝素和次牛磺酸的添加提高了体外含左旋肉碱培养基中成熟的绵羊卵母细胞受精率

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-01-17 DOI: 10.21157/j.ked.hewan.v16i4.27339

R. Rahmatullah, M. Setiadi, I. Supriatna

{"title":"HEPARIN AND HYPOTAURINE SUPPLEMENTATION IMPROVE THE FERTILIZATION RATE OF SHEEP OOCYTES MATURED IN MEDIA CONTAINING L-CARNITINE IN VITRO","authors":"R. Rahmatullah, M. Setiadi, I. Supriatna","doi":"10.21157/j.ked.hewan.v16i4.27339","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i4.27339","url":null,"abstract":"This study to do examine the effectiveness of using heparin and hypotaurine to improve the in vitro fertilization rate. In first stage, oocytes were matured in the maturation medium supplemented with 0.3 mg/mL L-carnitine to improve nuclear maturation. In second stage, matured oocytes were fertilized with semen incubated in fertilization medium supplemented with 0.1 IU/mL heparin and 10 µM hypotaurine. The final concentration of sperm used was 5x106 spermatozoa/mL, and its interaction with oocytes was carried out for 12 hours in 5% CO2 incubator at 38.5° C. The results showed that percentage of oocytes that reached metaphase II was significantly higher (P0.05) in media supplemented with 0.3 mg/mL L-carnitine compared to control (88.2±4.9% vs 79.8±5.1%). Furthermore, fertilization rate was characterized by formation of 2 PN was significantly higher (P0.05) in media supplemented with heparin and hypotaurine than control (82.6±2.0% vs 73.3±2.4%). In conclusion, L-carnitine supplementation at dose of 0.3 mg/mL on maturation media was effective in increasing percentage of matured oocytes. The combination of 0.1 IU/mL heparin and 10 µM hypotaurine in fertilization medium was also successful in elevating the percentage of fertilized oocytes.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125197003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS 利用快速DNA标记分析伊拉克地方山羊品种的遗传多样性

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2023-01-17 DOI: 10.21157/j.ked.hewan.v16i4.28579

A. Yousif, M. Abdalla

{"title":"GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS","authors":"A. Yousif, M. Abdalla","doi":"10.21157/j.ked.hewan.v16i4.28579","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i4.28579","url":null,"abstract":"Genetic diversity of three Iraqi local goat breeds (Black, Hybrid, and Meriz) was studied by using random amplified polymorphic DNA (RAPD) markers. Fifteen primers were used, they generated a total of 259 amplification products, from which 142 bands were polymorphic, revealing 55.29% polymorphism. It was found that the mean value of Nei’s gene diversity (h) and Shannon's ınformation index (I) were 0.1778 and 0.2546, respectively. According to the analysis of genetic distances among breeds, the highest genetic distance recorded between Black and Hybrid 0.5108, while the lowest genetic distance recorded between Hybrid and Meriz 0.1431. The phylogenetic dendrogram based on Nei’s genetic distance analysis was in agreement with their phenotypic similarities in which the tree separated the surveyed breeds into two clusters, the first one including Black goat breed, and the second cluster includes Hybrid and Meriz breeds. These results indicated that Black goat breed is most genetically distant from both Hybrid and Meriz breeds. Finally, the moderate value for both of polymorphism and genetic diversity which obtained from the present study indicate that these breeds are genetically different, which provide a good background about these breeds for genetic improvement and conservation of our local goat breeds in the future.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114435655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE BIOLOGICAL FUNCTIONS OF IMMUNOGLOBULIN Y (IgY) MOLECULES IN AGAINST INFECTION OF Enterococcus faecalis ORIGIN OF RED TILAPIA 免疫球蛋白Y (IgY)分子在红罗非鱼抗粪肠球菌感染中的生物学作用

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-09-08 DOI: 10.21157/j.ked.hewan.v16i3.24393

R. Rizkiantino, F. Pasaribu, R. D. Soejoedono, W. Arnafia, D. Reisinta, I. Wibawan

{"title":"THE BIOLOGICAL FUNCTIONS OF IMMUNOGLOBULIN Y (IgY) MOLECULES IN AGAINST INFECTION OF Enterococcus faecalis ORIGIN OF RED TILAPIA","authors":"R. Rizkiantino, F. Pasaribu, R. D. Soejoedono, W. Arnafia, D. Reisinta, I. Wibawan","doi":"10.21157/j.ked.hewan.v16i3.24393","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i3.24393","url":null,"abstract":"Red tilapia (Oreochromis hybrid) is Indonesia's leading freshwater fishery commodity susceptible to streptococcal bacterial infection. Many studies have been conducted on various efforts to prevent and treat this disease, one of which uses the immunoglobulin Y (IgY) molecule from chicken egg yolk. This study aimed to observe the biological function of IgY against Enterococcus faecalis as a cause of streptococcal-like infection. The agglutinin function was conducted by observing the growth of Enterococcus faecalis in brain heart infusion (BHI) broth media which was added with IgY suspension. The function of inhibin was performed using a spectrophotometric method to measure the level of turbidity of the bacterial suspension inoculated with IgY suspension. The bactericidal potential through the complementary activation pathway for red tilapia serum was carried out using a scanning electron microscope (SEM) method to evaluate the topography of the bacterial cell wall. The results of the study can be concluded that IgY anti-Enterococcus faecalis has the potential as an agglutinin, inhibin, and bactericidal agent through its putative potential in complement activation in streptococcal bacterial infections in red tilapia commodities.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"325 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127569416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA 乳酸林格蛋黄膨化剂中添加枣提取物对龙鸡精子解冻后品质的影响

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-09-08 DOI: 10.21157/j.ked.hewan.v16i3.24317

Nu'man - Hidayat, I. Ismoyowati, E. Tugiyanti, I. Suswoyo, R. Rosidi, I. H. Sulistyawan, Aras Prasetyo Nugroho

{"title":"DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA","authors":"Nu'man - Hidayat, I. Ismoyowati, E. Tugiyanti, I. Suswoyo, R. Rosidi, I. H. Sulistyawan, Aras Prasetyo Nugroho","doi":"10.21157/j.ked.hewan.v16i3.24317","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i3.24317","url":null,"abstract":"The research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.44±1.02%; 49.83±0.91%; 43.26±1.02%; and 45.33±0.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.21±0.43%; 35.62±0.51%; 30.54±0.60%; and 31.49±0.71% respectively), 0.5% (20.45±0.72%; 40.72±0.87%; 36.81±0.55%; and 38.56±0.63% respectively), 1% (25.68±0.93%; 46.27±0.75%; 40.52±1.02%; and 42.83±1.09% respectively) and 2% (22.55±0.92%; 43,71±0,74%; 38,84±0,71%; and 41,39±0,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126714338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET 饲喂不同水平鸡皮菌的地方肉鸡体重、肌重及胸肌蛋白、DNA和RNA含量的变化在发酵饮食中

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-09-08 DOI: 10.21157/j.ked.hewan.v16i3.25282

M. A. Yaman, S. Jeksi, Muhammad Daud

{"title":"BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET","authors":"M. A. Yaman, S. Jeksi, Muhammad Daud","doi":"10.21157/j.ked.hewan.v16i3.25282","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i3.25282","url":null,"abstract":"The study to evaluate changes in body weight, muscle weight, muscle protein content and total DNA and RNA on breast muscle of selectedlocal chicken due to different levels of fermented feed containing Kiapu (Pistia stratiotes L.) was conducted using a factorial randomized designfor 7 weeks. This study used 72 selected local chickens, which were separated into 2 groups (male and female) and fed on 3 types of feedtreatment, namely P0 (control treatment with 100% commercial feed), P1 (80% commercial feed + 20% fermented Kiapu feed), and P2 (70%commercial feed + 30% fermented Kiapu feed). Parameters were body weight, muscle weight, muscle protein content and total DNA and RNAon breast muscle of chicken. Total protein, DNA and RNA content were analysed by enzymatic reactions of prokaryotic cell and measured with aUV-Spectrophotometer according to the specified OD (optical density). The results showed that feeding Kiapu fermented 20-30% increased bodyweight and protein content of breast muscle of female chicken by change in DNA content. However, the feeding treatment had no changes inmuscle weight and RNA content of both in male and female chicken. On the other hand, substitution of 20-30% Kiapu fermented feed causednegative changes in DNA level of breast muscle. From this study, it was concluded that substitution of fermented feed containing 20-30% Kiapuassociated with the growth process of breast muscle protein is suitable for female but not suitable for male chicken.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131287674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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