BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET

M. A. Yaman, S. Jeksi, Muhammad Daud
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Abstract

The study to evaluate changes in body weight, muscle weight, muscle protein content and total DNA and RNA on breast muscle of selectedlocal chicken due to different levels of fermented feed containing Kiapu (Pistia stratiotes L.) was conducted using a factorial randomized designfor 7 weeks. This study used 72 selected local chickens, which were separated into 2 groups (male and female) and fed on 3 types of feedtreatment, namely P0 (control treatment with 100% commercial feed), P1 (80% commercial feed + 20% fermented Kiapu feed), and P2 (70%commercial feed + 30% fermented Kiapu feed). Parameters were body weight, muscle weight, muscle protein content and total DNA and RNAon breast muscle of chicken. Total protein, DNA and RNA content were analysed by enzymatic reactions of prokaryotic cell and measured with aUV-Spectrophotometer according to the specified OD (optical density). The results showed that feeding Kiapu fermented 20-30% increased bodyweight and protein content of breast muscle of female chicken by change in DNA content. However, the feeding treatment had no changes inmuscle weight and RNA content of both in male and female chicken. On the other hand, substitution of 20-30% Kiapu fermented feed causednegative changes in DNA level of breast muscle. From this study, it was concluded that substitution of fermented feed containing 20-30% Kiapuassociated with the growth process of breast muscle protein is suitable for female but not suitable for male chicken.
饲喂不同水平鸡皮菌的地方肉鸡体重、肌重及胸肌蛋白、DNA和RNA含量的变化在发酵饮食中
本试验采用因子随机设计,选取7周的地方鸡为试验对象,研究了饲粮中添加不同水平的紫皮鸡(Pistia stratiotes L.)发酵饲料对其体重、肌肉质量、肌肉蛋白含量以及胸肌总DNA和RNA的影响。本试验选用72只地方鸡,分为2组(公母),分别饲喂3种饲料处理,即P0(100%商品饲料对照处理)、P1(80%商品饲料+ 20%发酵基亚普饲料)和P2(70%商品饲料+ 30%发酵基亚普饲料)。参数为鸡体质量、肌肉质量、肌肉蛋白质含量、胸肌总DNA和RNAon。采用原核细胞酶促反应分析总蛋白、DNA和RNA含量,并按规定光密度用紫外分光光度计测定。结果表明,饲喂发酵过的佳普20 ~ 30%通过改变DNA含量提高了雌鸡体重和胸肌蛋白质含量。饲喂处理对鸡的肌肉质量和RNA含量无显著影响。另一方面,替代20-30%的基亚普发酵饲料对胸肌DNA水平产生负变化。综上所述,饲粮中添加20 ~ 30%基亚普与胸肌蛋白生长过程相关的发酵饲料可替代雌性鸡,但不适用于雄性鸡。
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