Ecological genetics最新文献

Erratum to “The strong base for using base editing in plants” (doi: 10.17816/ecogen567885) 对 "在植物中使用碱基编辑的坚实基础 "的勘误 (doi: 10.17816/ecogen567885)

Ecological genetics Pub Date : 2024-05-14 DOI: 10.17816/ecogen630239

引用次数: 0

Hairy roots biochemical characteristics of vegetable pea’s morphotype with modified leaf 具有改良叶片的菜豆形态的毛根生化特征

Ecological genetics Pub Date : 2024-05-14 DOI: 10.17816/ecogen622926

O. Timina, O. Timin, A. Stepanova

{"title":"Hairy roots biochemical characteristics of vegetable pea’s morphotype with modified leaf","authors":"O. Timina, O. Timin, A. Stepanova","doi":"10.17816/ecogen622926","DOIUrl":"https://doi.org/10.17816/ecogen622926","url":null,"abstract":"BACKGROUND: In the literature, data on the successful production of high-protein pea root cultures with mutant alleles tl and af tl and their biochemical characteristics are not presented. \u0000AIM: The aim of this study is the biochemical characteristic of the obtained transformed cultures of mutant pea lines with a modified leaf morphotype was. Rol genes clarification of transformed pea mutant lines by wild strains of Agrobacterium rhizogenes and amino acid analysis of the total protein of the obtained roots cultures were objectives of the research. \u0000MATERIALS AND METHODS: Total DNA has been isolated from pea mutant root cultures. The studies were carried out on the equipment of the thermal cycler Tertsik of the company DNA-Technology (Russia). Amplicons were detected by electrophoresis in a 2% agarose gel. The gel was visualized and photographed under ultraviolet light (λ = 312 nm). The quantitative and qualitative amino acid composition of root cultures was determined by ion exchange chromatography on the amino acid analyzer AAA-339 (Microtechna, Czech Republic). \u0000RESULTS: PCR analysis revealed the absence of agrobacterial contamination in the transformed cultures and their stable growth on liquid and agar hormone-free media for 5 years. PCR assay showed the presence of rol C and D genes in culture with the tltl genotype and four rol genes A, B, C, and D in culture with the afaftltl genotype. A differentiated content of a number of amino acids in the biomass of transformed cultures was found depending on the genotype of the culture and the insertion of rol genes. Seven essential amino acids were identified in both cultures. \u0000CONCLUSIONS: Tryptophan turned out to be the limiting essential amino acid for both cultures. In terms of the sum of essential, ketogenic and sulfur-containing amino acids, the culture with the rol genes A, B, C, and D turned out to be the richest and most balanced.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"52 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140980996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

CRISPR/Cas editing of a CPC gene in Arabidopsis thaliana 拟南芥中 CPC 基因的 CRISPR/Cas 编辑

Ecological genetics Pub Date : 2024-05-14 DOI: 10.17816/ecogen624373

E. A. Khusnutdinov, Maria A. Panfilova, M. Terekhov, E. Mikhaylova

{"title":"CRISPR/Cas editing of a CPC gene in Arabidopsis thaliana","authors":"E. A. Khusnutdinov, Maria A. Panfilova, M. Terekhov, E. Mikhaylova","doi":"10.17816/ecogen624373","DOIUrl":"https://doi.org/10.17816/ecogen624373","url":null,"abstract":"BACKGROUND: Identification of target genes responsible for visible phenotypic effect may contribute to the development of transgene-free bioengineering strategies and application of crop varieties with edited genome. CAPRICE (CPC) is a single-repeat R3 MYB transcription factor, involved in anthocyanin biosynthesis and trichome formation. It is assumed that CPC controls the expression of Dihydroflavonol-4-reductase (DFR), a key gene of anthocyanin biosynthesis. \u0000AIM: The aim of the study was to determine whether knockout of the CPC gene using CRISPR/Cas9 results in visible anthocyanin accumulation. \u0000MATERIALS AND METHODS: Three guide RNAs were designed to excise a MYB domain from the CPC gene of Arabidopsis thaliana. Anthocyanin content and expression of CPC and DFR genes were studied in edited plants. \u0000RESULTS: The expected 662 bp deletion was detected in 2,7% of glufosinate-resistant plants, however none of the mutations were homozygous. Four edited lines were studied in four generations. An upregulation of the DFR gene was observed in edited lines, however CPC gene expression, anthocyanin content and trichome development were not significantly different from those in control plants. Moreover, in A. thaliana pigmentation did not directly depend on DFR or CPC gene expression. \u0000CONCLUSIONS: Our results suggest that CPC gene is involved in regulation of DFR gene expression and anthocyanin biosynthesis pathway, however in case of mutations plants might utilize other transcription factors to maintain homeostasis. Therefore, CPC gene is not a suitable target for CRISPR/Cas studies in Arabidopsis.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"19 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140980114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PCR-based genome walking methods (review) 基于 PCR 的基因组行走方法（综述）

Ecological genetics Pub Date : 2024-05-14 DOI: 10.17816/ecogen624820

Elena S. Okulova, M. Burlakovskiy, L. A. Lutova

引用次数: 1

Ecological genetics. What is it? 20 years later 生态遗传学。它是什么？20 年后

Ecological genetics Pub Date : 2024-02-13 DOI: 10.17816/ecogen625425

Sergei G. Inge-Vechtomov, L. Barabanova

引用次数: 0

Ecological genetics. What is it? 20 years later 生态遗传学。它是什么？20 年后

Ecological genetics Pub Date : 2024-02-13 DOI: 10.17816/ecogen625425

Sergei G. Inge-Vechtomov, L. Barabanova

引用次数: 0

M. E. Lobashev’s physiological theory of the mutation process and the formation of contemporary views on mutational changes in genetic material M.洛巴舍夫的突变过程生理学理论和当代遗传物质突变观点的形成

Ecological genetics Pub Date : 2023-12-14 DOI: 10.17816/ecogen623886

A. Zhuk, E. Stepchenkova, S. G. Inge-Vechtomov

{"title":"M. E. Lobashev’s physiological theory of the mutation process and the formation of contemporary views on mutational changes in genetic material","authors":"A. Zhuk, E. Stepchenkova, S. G. Inge-Vechtomov","doi":"10.17816/ecogen623886","DOIUrl":"https://doi.org/10.17816/ecogen623886","url":null,"abstract":"Changes in mutation rates can significantly impact population size and its genetic structure, leading to the emergence of new traits and species. At the same time, the destabilization of genetic material is the main cause of hereditary and oncological diseases and aging. M.E. Lobashev was the first to point out the connection between mutations and repair. He introduced the concept of a premutation state or primary lesion of genetic material and suggested that mutagenesis is a physiological process in which mutations occurs during the repair of damaged genetic material due to non-identical restoration of its structure. The theories of M. E. Lobashev laid the groundwork for understanding the causes and mechanisms of inherited changes in genetic material, which have been experimentally confirmed in studies of replication, repair, and recombination. It is now known that mutations arise through a multistep process over time, due to ambiguity of one of template processes – DNA synthesis. Recent research made it possible to establish the physical nature of primary lesions and mutations, to develop various methods for their identification, and estimate the impact of primary lesions and mutations in the phenotype formation.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"19 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138971000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Models of osmotic stress as a tool for proteomics and metabolomics of legume seeds 作为豆科植物种子蛋白质组学和代谢组学工具的渗透胁迫模型

Ecological genetics Pub Date : 2023-12-13 DOI: 10.17816/ecogen611119

T. Leonova, T. Bilova, Andrej Frolov

{"title":"Models of osmotic stress as a tool for proteomics and metabolomics of legume seeds","authors":"T. Leonova, T. Bilova, Andrej Frolov","doi":"10.17816/ecogen611119","DOIUrl":"https://doi.org/10.17816/ecogen611119","url":null,"abstract":"Drought poses a significant challenge to the sustainable development of modern agriculture and to the achievement of high crop yields. Water deficit causes osmotic stress and triggers plant physiological responses characterized by reduced water potential, diminished stomatal conductance, and decreased photosynthetic efficiency. Long-term adaptation to osmotic stress entails intricate metabolic rearrangements, leading to the accumulation of osmoprotectants, activation of antioxidant systems, and increased biosynthesis of stress-protective proteins. The severity and duration of drought, along with plant genotype and developmental stage, influence the plant response to stress, consequently affecting crop yield and quality. Particularly in the context of legumes, which are crucial for human and animal nutrition, understanding adaptive strategies to water deficit is essential for the cultivation of drought-resistant genotypes, primarily because these crops predominantly thrive in semi-arid regions. Proteomics and metabolomics approaches, in turn, serve as valuable tools, offering critical insights into the molecular dynamics governing plant responses to drought stress. Furthermore, the use of reliable drought simulation models is imperative for the effective evaluation of legume response to water scarcity, aiding the cultivation of drought-tolerant varieties. This review highlights the perspectives of utilizing different osmotic stress models to investigate proteome and metabolome alteration within seeds of food legumes.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"14 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139003721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental genetics and predictive medicine: 20 years later. New trends and old basis 环境遗传学和预测医学：20 年之后。新趋势和旧基础

Ecological genetics Pub Date : 2023-12-07 DOI: 10.17816/ecogen623390

A. Glotov

{"title":"Environmental genetics and predictive medicine: 20 years later. New trends and old basis","authors":"A. Glotov","doi":"10.17816/ecogen623390","DOIUrl":"https://doi.org/10.17816/ecogen623390","url":null,"abstract":"This review, based on previously formulated hypotheses, our own research and assumptions, examines the main modern trends and directions of molecular medicine related to the assessment of the effect of environmental factors on the human genome, that is, the problem of human environmental genetics. Among them, special attention is paid to aspects of genome sequencing, from the draft version to the final version, and the start of the Pangenome project. Information is provided on all programs for analyzing the human genome, on the connection between biobanks and national genomic projects, on the importance of genetic diversity and genome errors, on its polymorphism and the importance of genomic clinical databases. Ten trends in molecular medicine over the past 20 years are separately highlighted. The review presents new challenges in predictive medicine related to carrier screening and the development of preimplantation genetic testing for both monogenic and multifactorial diseases. The situation with genetic testing related to an individual's susceptibility to environmental factors and diseases is summarized. There has been an accumulation of a huge number of research results. Some attention is paid to pharmacogenetic studies, for which certain levels of evidence have been formed in the clinic, which have led in some countries to the start of projects for the development and implementation of pharmacogenetic passports. Some new areas are considered - psychogenetics, sports genetics, genetics of susceptibility to coronavirus infection and others. In the final parts, attention is paid to the genetic prognosis of multifactorial diseases, which is based on both DNA and RNA studies (and other omics technologies) using mathematical modeling and the creation of a new paradigm of a genetic passport - an omics map of reproductive health.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"59 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic polymorphism in waxweed (Red Book of the Russian Federation) in North-West Russia 俄罗斯西北部蜡菊（《俄罗斯联邦红皮书》）的遗传多态性

Ecological genetics Pub Date : 2023-12-07 DOI: 10.17816/ecogen624197

Oleg Tikhodeyev, Marina Tikhodeeva, Anna Romanovich, Ulyana Galaktionova, Olga Semicheva, Vyacheslav Bolshakov

{"title":"Genetic polymorphism in waxweed (Red Book of the Russian Federation) in North-West Russia","authors":"Oleg Tikhodeyev, Marina Tikhodeeva, Anna Romanovich, Ulyana Galaktionova, Olga Semicheva, Vyacheslav Bolshakov","doi":"10.17816/ecogen624197","DOIUrl":"https://doi.org/10.17816/ecogen624197","url":null,"abstract":"Background. Waxweed (Myrica gale L.) is a protected plant species found in the Russian Federation only in the Leningrad District and Karelia. It is almost not studied from the genetic point of view. This species is presumably hexaploid; it propagates mainly vegetatively, and the role of sexual reproduction in the life cycle of waxweed is unclear. All waxweed populations in the Russian Federation are small and belong to the edge ones (occupy the easternmost positions in the range). The aim of the study: to investigate genomic DNA polymorphism in waxweed populations in the Russian Federation, and to evaluate the role of sexual reproduction in propagation and dispersal of this species. Materials and methods. AFLP-analysis of waxweed genomic DNA in 6 natural populations (5 populations in the Leningrad District, 1 in Karelia). Using 3 pairs of primers, we studied waxweed genomic polymorphism for 22 DNA fragments. Results. Each of the studied waxweed populations is genetically polymorphic. Among 182 analyzed plants we distinguished 27 different AFLP-genotypes, two of which were common in all populations studied. Most of others AFLP genotypes (20) were represented just by a single plant or a couple of plants. Some of these rare AFLP genotypes are likely the results of mutation and/or recombination processes affecting the common AFLP genotypes. Conclusion. The role of sexual reproduction in waxweed propagation, although minor, is noticeable.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"36 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138593500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0