Genetic polymorphism in waxweed (Red Book of the Russian Federation) in North-West Russia

Q3 Agricultural and Biological Sciences

Ecological genetics Pub Date : 2023-12-07 DOI:10.17816/ecogen624197

Oleg Tikhodeyev, Marina Tikhodeeva, Anna Romanovich, Ulyana Galaktionova, Olga Semicheva, Vyacheslav Bolshakov

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Abstract

Background. Waxweed (Myrica gale L.) is a protected plant species found in the Russian Federation only in the Leningrad District and Karelia. It is almost not studied from the genetic point of view. This species is presumably hexaploid; it propagates mainly vegetatively, and the role of sexual reproduction in the life cycle of waxweed is unclear. All waxweed populations in the Russian Federation are small and belong to the edge ones (occupy the easternmost positions in the range). The aim of the study: to investigate genomic DNA polymorphism in waxweed populations in the Russian Federation, and to evaluate the role of sexual reproduction in propagation and dispersal of this species. Materials and methods. AFLP-analysis of waxweed genomic DNA in 6 natural populations (5 populations in the Leningrad District, 1 in Karelia). Using 3 pairs of primers, we studied waxweed genomic polymorphism for 22 DNA fragments. Results. Each of the studied waxweed populations is genetically polymorphic. Among 182 analyzed plants we distinguished 27 different AFLP-genotypes, two of which were common in all populations studied. Most of others AFLP genotypes (20) were represented just by a single plant or a couple of plants. Some of these rare AFLP genotypes are likely the results of mutation and/or recombination processes affecting the common AFLP genotypes. Conclusion. The role of sexual reproduction in waxweed propagation, although minor, is noticeable.

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俄罗斯西北部蜡菊（《俄罗斯联邦红皮书》）的遗传多态性

背景。黄蜡草(Myrica gale L.)是俄罗斯联邦仅在列宁格勒地区和卡累利阿发现的受保护植物物种。几乎没有人从遗传学的角度来研究它。这个物种大概是六倍体;主要以营养繁殖为主，有性繁殖在黄蜡草生命周期中的作用尚不清楚。俄罗斯联邦的所有黄蜡草种群都很小，属于边缘种群(占据范围的最东端)。本研究的目的是:研究俄罗斯联邦黄蜡草种群的基因组DNA多态性，并评价有性生殖在该物种的繁殖和传播中的作用。材料和方法。6个自然居群(列宁格勒5个，卡累利阿1个)黄蜡草基因组DNA的aflp分析。利用3对引物，对22个DNA片段进行了基因组多态性分析。结果。每一个被研究的蜡草种群都具有基因多态性。在182种分析植物中，我们区分出27种不同的aflp基因型，其中两种在所有研究群体中都是常见的。大多数其他AFLP基因型(20个)仅由单个植株或一对植株代表。其中一些罕见的AFLP基因型可能是影响常见AFLP基因型的突变和/或重组过程的结果。结论。有性繁殖在黄蜡草繁殖中的作用，虽然很小，但很明显。

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来源期刊

Ecological genetics Environmental Science-Ecology

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.