Drug metabolism and personalized therapy最新文献_第10页

Datura stramonium abrogates depression- and anxiety-like disorders in mice: possible involvement of monoaminergic pathways in its antidepressant activity 曼陀罗能消除小鼠的抑郁和焦虑样障碍:其抗抑郁活性可能涉及单胺能途径

Drug metabolism and personalized therapy Pub Date : 2022-02-24 DOI: 10.1515/dmpt-2021-0166

M. A. Akanji, Oyinloye O. Elijah, Alabi A. Oyedolapo, Aderionla A. Aderonke, Ogunjimi L. Opeoluwa, A. Omoloye, F. Oladoja, S. O. Olatundun, Osipitan L. Edatomolaosi

{"title":"Datura stramonium abrogates depression- and anxiety-like disorders in mice: possible involvement of monoaminergic pathways in its antidepressant activity","authors":"M. A. Akanji, Oyinloye O. Elijah, Alabi A. Oyedolapo, Aderionla A. Aderonke, Ogunjimi L. Opeoluwa, A. Omoloye, F. Oladoja, S. O. Olatundun, Osipitan L. Edatomolaosi","doi":"10.1515/dmpt-2021-0166","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0166","url":null,"abstract":"Abstract Objectives Ethno-botanical surveys uncovered the use of Datura stramonium in the management of mental health abnormalities. Antidepressant- and anxiolytic-like activities of hydroethanol leaf extract of D. stramonium (HLDS) in mice and its possible mechanism of action were investigated in this study. Methods The hole-board test (HBT), open field test (OFT), elevated plus maze test (EPMT), and social interaction test (SIT) were used to investigate the anxiolytic-like activity while forced swim test (FST) and tail suspension test (TST) were employed for the antidepressant effect. Mice were pre-treated orally with purified water (10 mL/kg), bromazepam (1 mg/kg), fluoxetine (20 mg/kg) and D. stramonium (25, 50, 100 and 200 mg/kg). One hour post-treatment, mice were subjected to the various tests. Results In HBT, D. stramonium increased the head dips and sectional crossings turnover. D. stramonium increased the number of square crossed and rearings/assisted rearings in OFT. DS increased the time spent in open arms of EPM. In SIT, D. stramonium increased the frequency/duration of interactions. In FST and TST, D. stramonium decreased the duration of immobility which were reversed by doxazosin (α1 adrenoceptor antagonist) and ondasentron (5-HT3 receptor antagonist). Conclusions HLDS has anxiolytic- and antidepressant-like activities through modulation of serotoninergic and adrenergic neurotransmissions.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81021755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

CYP450 2D6 and 2C19 genotypes in ADHD: not related with treatment resistance but with over-representation of 2C19 ultra-metabolizers ADHD患者CYP450 2D6和2C19基因型:与治疗耐药无关，但与2C19超代谢物的过度表达有关

Drug metabolism and personalized therapy Pub Date : 2022-02-24 DOI: 10.1515/dmpt-2021-0163

M. Kutuk, A. Tufan, Z. Topal, Ufuk Acikbas, G. Guler, Bahriye Karakaş, H. Basaga, F. Kılıçaslan, E. Altıntaş, Yeliz Aka, O. Kutuk

{"title":"CYP450 2D6 and 2C19 genotypes in ADHD: not related with treatment resistance but with over-representation of 2C19 ultra-metabolizers","authors":"M. Kutuk, A. Tufan, Z. Topal, Ufuk Acikbas, G. Guler, Bahriye Karakaş, H. Basaga, F. Kılıçaslan, E. Altıntaş, Yeliz Aka, O. Kutuk","doi":"10.1515/dmpt-2021-0163","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0163","url":null,"abstract":"Abstract Objectives Cytochrome P450 (CYP450) is a major enzyme system involved in drug metabolism as well as regulation of brain function. Although individual variability in CYP enzymes have been studied in terms of personality traits and treatment effects, no study up to now evaluated CYP polymorphisms in children with attention deficit/hyperactivity disorder (ADHD). We aimed to define the genetic profiles of CYP2D6 and CYP2C19 relevant alleles in children with ADHD according to treatment status and compare the frequencies according to past results. Methods Three hundred and seventeen patients with ADHD-Combined Presentation were enrolled; symptom severity was evaluated by parents and clinicians while adverse effects of previous treatments were evaluated with parent and child reports. Reverse blotting on strip assays was used for genotyping and descriptive and bivariate analyses were conducted. A p-value was set at 0.05 (two-tailed). Results Children were divided into treatment-naïve (n=194, 61.2%) and treatment-resistant (n=123, 38.8%) groups. Within the whole sample PM, EM and UM status according to 2D6 were 3.8% (n=12), 94.3% (n=299) and 21.9% (n=6); respectively. PM, IM, EM and UM status according to 2C19 were 2.5% (n=8), 19.8% (n=63), 48.6% (n=154) and 29.0% (n=92), respectively. No relationship with treatment resistance, comorbidity or gender could be found. Importantly, CYP2C19 UMs were significantly more frequent in ADHD patients compared to previous studies in the general population. Conclusions CYPs may be a rewarding avenue of research to elucidate the etiology and treatment of patients with ADHD.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90654511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Factors influencing methotrexate and methotrexate polyglutamate in patients with rheumatoid arthritis: a systematic review of population pharmacokinetics 类风湿关节炎患者甲氨蝶呤和甲氨蝶呤聚谷氨酸的影响因素:人群药代动力学的系统综述

Drug metabolism and personalized therapy Pub Date : 2022-02-24 DOI: 10.1515/dmpt-2021-0190

Janthima Methaneethorn, Rowan Alejielat, Nattawut Leelakanok

{"title":"Factors influencing methotrexate and methotrexate polyglutamate in patients with rheumatoid arthritis: a systematic review of population pharmacokinetics","authors":"Janthima Methaneethorn, Rowan Alejielat, Nattawut Leelakanok","doi":"10.1515/dmpt-2021-0190","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0190","url":null,"abstract":"Abstract Low dose methotrexate (MTX) is commonly used in the treatment of rheumatoid arthritis. The clinical effect is mediated by its metabolite, methotrexate polyglutamate (MTX-PGn). The drug exhibits high interindividual pharmacokinetic variability and the optimal MTX dose is different among individuals. Thus, several MTX population pharmacokinetic (PopPK) models were developed to characterize factors affecting MTX pharmacokinetic variability. This review summarizes significant predictors for MTX pharmacokinetics and identifies knowledge gaps to be further examined. A total of 359 articles were identified from a systematic search of four databases: PubMed, Science Direct, and CINAHL Complete. Of these eight studies were included. Most studies investigated influential factors on MTX pharmacokinetics, but information on MTX-PGn is limited, with only one study performing a parent-metabolite (MTX-PG3) model. MTX pharmacokinetics was described using a two-compartment model with first-order elimination in most studies, with the MTX clearance ranging from 6.94 to 12.39 L/h. Significant predictors influencing MTX clearance included weight, creatinine clearance, sex, OATP1B3 polymorphism, and MTX multiple dosing. While body mass index and red blood cell counts were significant predictors for MTX-PG3 clearance. Providing that MTX-PGn plays a crucial role in clinical effect, further studies should determine other factors affecting MTX-PGn as well as its relationship with clinical response.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83594337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the stability of furosemide in tablet form during six-month storage in spaceflight and peculiarities of its pharmacokinetics and pharmacodynamics under conditions of anti-orthostatic hypokinesia 航天飞行6个月贮存期速尿片剂稳定性评价及抗直立性运动障碍条件下药代动力学和药效学特点

Drug metabolism and personalized therapy Pub Date : 2022-02-24 DOI: 10.1515/dmpt-2021-0149

A. V. Polyakov, Andreу A. Svistunov, S. Kondratenko, I. Kovachevich, Lyudmila G. Repenkovа, Marina I. Savelyevа, E. Shikh, V. Noskov

{"title":"Evaluation of the stability of furosemide in tablet form during six-month storage in spaceflight and peculiarities of its pharmacokinetics and pharmacodynamics under conditions of anti-orthostatic hypokinesia","authors":"A. V. Polyakov, Andreу A. Svistunov, S. Kondratenko, I. Kovachevich, Lyudmila G. Repenkovа, Marina I. Savelyevа, E. Shikh, V. Noskov","doi":"10.1515/dmpt-2021-0149","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0149","url":null,"abstract":"Abstract Objectives The present study investigated the stability of furosemide under space-flight conditions on board the International Space Station, as well as its pharmacokinetics and pharmacodynamics under conditions simulating exposure to some space-flight factors. Methods Quantitative analysis of furosemide tablets by HPLC was performed before spaceflight (background), then after six months storage under normal ground conditions (control) and under spaceflight conditions (SF). The pharmacokinetics and pharmacodynamics of furosemide were studied in six healthy volunteers after a single oral dose of 40 mg under normal conditions (background) and under anti-orthostatic hypokinesia (ANOH). Results Quantitative content of furosemide in tablets before SF was 40.19 ± 0.28 mg (100.47 ± 0.71%), after 6 months storage: under normal conditions (control) – 39.9 ± 0.39 mg (99.73 ± 0.98%), under SF – 39.24 ± 0.72 mg (98.11 ± 1.80%), which was within the prescribed limits. Studying basic hemodynamic parameters showed that in ANOH conditions 6 h after furosemide administration there was a statistically significant increase of the stroke volume (SV) (+36.5 Δ%), a tendency for increasing of the stroke index (SI) (+36.5 Δ%) and decreasing of the total peripheral resistance (TPR) (−21.9 Δ%) compared to baseline study. Conclusions It has been established that various factors of space flight (overloading, excessive vibration, microgravity, etc.) do not negatively influence the stability of furosemide in tablet form during storage for 6 months on board the International Space Station.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85326075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cnestis ferruginea Vahl ex DC (Connaraceae) downregulates expression of immediate early genes in kainic acid-induced temporal lobe epilepsy in mice. conaraceae植物Cnestis ferruginea Vahl ex DC下调kainic酸诱导小鼠颞叶癫痫的直接早期基因表达。

Drug metabolism and personalized therapy Pub Date : 2021-12-09 DOI: 10.1515/dmpt-2021-0145

Emmanuel S Ojo, Ismail O Ishola, Olasunmbo Afolayan, Ayorinde B James, Benneth Ben-Azu, Olufunmilayo O Adeyemi

{"title":"Cnestis ferruginea Vahl ex DC (Connaraceae) downregulates expression of immediate early genes in kainic acid-induced temporal lobe epilepsy in mice.","authors":"Emmanuel S Ojo, Ismail O Ishola, Olasunmbo Afolayan, Ayorinde B James, Benneth Ben-Azu, Olufunmilayo O Adeyemi","doi":"10.1515/dmpt-2021-0145","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0145","url":null,"abstract":"Objectives: This study investigates the influence of Cnestis ferruginea (CF) on kainic acid (KA)-induced immediate early genes (IEGs) associated with hippocampal sclerosis in temporal lobe epilepsy (TLE) in mice.Methods: Animals were randomly divided into preventive treatment; vehicle (10 mL/kg, p.o.) or CF (400 mg/kg, p.o.) for three consecutive days before KA (5 mg/kg, i.p.) on days 4 and 5. In the reversal model, KA (5 mg/kg, i.p.) was administered on days 1 and 2 before CF (400 mg/kg) administration on days 3-5. Animals were euthanized on day 5, 6 h after KA exposure in preventive model and 1 h after CF administration in reversal model to estimate markers of IEGs.Results: KA upregulated the expression of c-Fos protein by 3.32-, 9.45-, 8.13-, and 8.66-fold in the hippocampal CA1, CA2, CA3, and DG regions, respectively. Also, KA elevated inducible nitric oxide synthase protein expression by 10.9-, 10.6-, 9.78-, and 9.51-fold. Besides, mRNA expression of brain-derived neurotrophic factors and heat shock protein was increased by 2.38- and 1.39-fold, respectively, after exposure to KA which were attenuated by CF.Conclusions: CF attenuated KA-induced IEGs and could be used as an adjunct in TLE.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39958626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virgin coconut oil abrogates depression-associated cognitive deficits by modulating hippocampal antioxidant balance, GABAergic and glutamatergic receptors in mice. 初榨椰子油通过调节小鼠海马抗氧化平衡、gaba能和谷氨酸能受体来消除抑郁症相关的认知缺陷。

Drug metabolism and personalized therapy Pub Date : 2021-12-09 DOI: 10.1515/dmpt-2021-0126

Edem Ekpenyong Edem, Blessing Eghosa Ihaza, Adedamola Adediran Fafure, Azeez Olakunle Ishola, Kate Eberechukwu Nebo, Linus Anderson Enye, Elizabeth Toyin Akinluyi

{"title":"Virgin coconut oil abrogates depression-associated cognitive deficits by modulating hippocampal antioxidant balance, GABAergic and glutamatergic receptors in mice.","authors":"Edem Ekpenyong Edem, Blessing Eghosa Ihaza, Adedamola Adediran Fafure, Azeez Olakunle Ishola, Kate Eberechukwu Nebo, Linus Anderson Enye, Elizabeth Toyin Akinluyi","doi":"10.1515/dmpt-2021-0126","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0126","url":null,"abstract":"Objectives: GABA and glutamate neurotransmission play critical roles in both the neurobiology of depression and cognition; and Virgin coconut oil (VCO) is reported to support brain health. The present study investigated the effect of VCO on depression-associated cognitive deficits in mice.Methods: Thirty male mice divided into five groups were either exposed to chronic unpredicted mild stress (CUMS) protocol for 28 days or pre-treated with 3 mL/kg b. wt. of VCO for 21 days or post-treated with 3 mL/kg b. wt. of VCO for 21 days following 28 days of CUMS exposure. Mice were subjected to behavioural assessments for depressive-like behaviours and short-term memory, and thereafter euthanised. Hippocampal tissue was dissected from the harvested whole brain for biochemical and immunohistochemical evaluations.Results: Our results showed that CUMS exposure produced depressive-like behaviours, cognitive deficits and altered hippocampal redox balance. However, treatment with VCO abrogated depression-associated cognitive impairment, and enhanced hippocampal antioxidant concentration. Furthermore, immunohistochemical evaluation revealed significant improvement in GABAA and mGluR1a immunoreactivity following treatment with VCO in the depressed mice.Conclusions: Therefore, findings from this study support the dietary application of VCO to enhance neural resilience in patients with depression and related disorders.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39704836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Modifying effects of TNF-α, IL-6 and VDR genes on the development risk and the course of COVID-19. Pilot study. TNF-α、IL-6和VDR基因对COVID-19发生风险和病程的调节作用试点研究。

Drug metabolism and personalized therapy Pub Date : 2021-12-06 DOI: 10.1515/dmpt-2021-0127

Liliia Fishchuk, Zoia Rossokha, Valeriy Pokhylko, Yuliia Cherniavska, Svitlana Tsvirenko, Serhii Kovtun, Nataliia Medvedieva, Viktoriia Vershyhora, Nataliia Gorovenko

{"title":"Modifying effects of TNF-α, IL-6 and VDR genes on the development risk and the course of COVID-19. Pilot study.","authors":"Liliia Fishchuk, Zoia Rossokha, Valeriy Pokhylko, Yuliia Cherniavska, Svitlana Tsvirenko, Serhii Kovtun, Nataliia Medvedieva, Viktoriia Vershyhora, Nataliia Gorovenko","doi":"10.1515/dmpt-2021-0127","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0127","url":null,"abstract":"Objectives: COVID-19 continues to range around the world and set morbidity and mortality antirecords. Determining the role of genetic factors in the development of COVID-19 may contribute to the understanding of the pathogenetic mechanisms that lead to the development of complications and fatalities in this disease. The aim of our study was to analyze the effect of TNF-α (rs1800629), IL-6 (rs1800795) and VDR (rs731236 and rs1544410) genes variants on the development risk and the course of COVID-19 in intensive care patients.Methods: The study group included 31 patients with diagnosis \"viral COVID-19 pneumonia\". All patients underwent standard daily repeated clinical, instrumental and laboratory examinations. Determination of IL-6, TNF-α, and VDR genes variants was performed using the PCR-RFLP method.Results: It was found a significant increase in the rate of the CC genotype and C allele (38.7 vs. 12.0% and 0.6 vs. 0.4%, respectively) of the IL-6 gene in all patients of the study in comparison with population frequencies. There was a significantly higher rate of heterozygous genotypes TC and GA of the VDR gene in group of died patients. The rs1800629 variant of the TNF-α gene is associated with the need for respiratory support and its longer duration in patients with COVID-19.Conclusions: The obtained results support a hypothesis about the influence of variants of IL-6, TNF-α and VDR genes on severity of COVID-19. However, in order to draw definite conclusions, further multifaceted research in this area are need.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39941612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Predicting drug-drug interactions by electrochemically driven cytochrome P450 3A4 reactions. 通过电化学驱动细胞色素P450 3A4反应预测药物-药物相互作用。

Drug metabolism and personalized therapy Pub Date : 2021-12-06 eCollection Date: 2022-09-01 DOI: 10.1515/dmpt-2021-0116

Victoria V Shumyantseva, Polina I Koroleva, Tatiana V Bulko, Gennady V Sergeev, Sergei A Usanov

{"title":"Predicting drug-drug interactions by electrochemically driven cytochrome P450 3A4 reactions.","authors":"Victoria V Shumyantseva, Polina I Koroleva, Tatiana V Bulko, Gennady V Sergeev, Sergei A Usanov","doi":"10.1515/dmpt-2021-0116","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0116","url":null,"abstract":"Objectives: Human cytochrome P450 3A4 is the most abundant hepatic and intestinal Phase I enzyme that metabolizes approximately 60% marketed drugs. Simultaneous administration of several drugs may result in appearance of drug-drug interaction. Due to the great interest in the combination therapy, the exploration of the role of drug as \"perpetrator\" or \"victim\" is important task in pharmacology. In this work the model systems based on electrochemically driven cytochrome P450 3A4 for the analysis of drug combinations was used. We have shown that the analysis of electrochemical parameters of cytochrome P450 3A4 and especially, potential of the start of catalysis, Eonset, possess predictive properties in the determination of the leading (\"perpetrator\") properties of drug. Based on these experimental data, we concluded, that the more positive potential of the start of catalysis, Eonset, the more pronounced the role of drug as leading medication.Methods: Electrochemically driven cytochrome P450 3A4 was used as probe and measuring tool for the estimation of the role of interacting drugs.Results: It is shown that the electrochemical non-invasive model systems for monitoring the catalytic activity of cytochrome P450 3A4 can be used as prognostic devise in assessment of drug/drug interacting medications.Conclusions: Cytochrome P450 3A4 activity was studied in electrochemically driven system. Method was implemented to monitor drug/drug interactions. Based on the obtained experimental data, we can conclude that electrochemical parameter such as potential of onset of catalysis, Eonset, has predictive efficiency in assessment of drug/drug interacting medications in the case of the co-administration.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39941613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of ABCB1 3435C>T polymorphism in the Cuban population. ABCB1 3435C>T多态性在古巴人群中的流行

Drug metabolism and personalized therapy Pub Date : 2021-12-06 DOI: 10.1515/dmpt-2020-0156

Idania Rodeiro Guerra, Jose Herrea, Elizabeth Cuétara, Gabino Garrido, Elizabeth Reyes, Ioanna Martínez, Carlos L Pérez, Gisselle Fernández, Ivones Hernández-Balmaseda, René Delgado, Julia C Stingl, Wim Vanden Berghe

{"title":"Prevalence of ABCB1 3435C>T polymorphism in the Cuban population.","authors":"Idania Rodeiro Guerra, Jose Herrea, Elizabeth Cuétara, Gabino Garrido, Elizabeth Reyes, Ioanna Martínez, Carlos L Pérez, Gisselle Fernández, Ivones Hernández-Balmaseda, René Delgado, Julia C Stingl, Wim Vanden Berghe","doi":"10.1515/dmpt-2020-0156","DOIUrl":"https://doi.org/10.1515/dmpt-2020-0156","url":null,"abstract":"Objectives: ABCB1 gene polymorphisms can modify P-glycoprotein function with clinical consequences.Methods: The 3435C>T polymorphism prevalence was analyzed using oligonucleotide probes and next-generation sequencing in 421 unrelated healthy individuals living in Cuba. Data were stratified by gender, ethnic background and residence. The genotype and allelic frequencies were determined.Results: The genotype distribution met the Hardy-Weinberg equilibrium assumption. The allelic frequency was 63.5% for the 3435C variant. The genotype frequencies were 41.1% for CC, 44.9% for CT and 14.0% for TT. The allele and genotype distributions differed between individuals living in La Habana and Santiago de Cuba (p<0.05) when ethnic background was analyzed. The allelic distribution was similar among Admixed and Black subjects, and they differed from Caucasians. The CC genotype was equally distributed among Admixed and Black subjects, and they differed from Caucasians. The TT genotype frequency differed between Caucasians and Admixed. The CT genotype was distributed differently among the three groups. Similar distribution was obtained in Brazilians, whereas some similarities were observed in African, Spanish and Chinese populations, consistent with the mixed Cuban ethnic origin.Conclusions: This is the first report on allele and genotype frequencies of the 3435C>T polymorphism in Cuba, which may support personalized medicine programs.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39941611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic polymorphisms of reproductive hormones and their receptors in assisted reproduction technology for patients with polycystic ovary syndrome. 多囊卵巢综合征患者辅助生殖技术中生殖激素及其受体的遗传多态性

Drug metabolism and personalized therapy Pub Date : 2021-12-01 DOI: 10.1515/dmpt-2021-0123

Yulia A Koloda, Yulia V Denisova, Natalia M Podzolkova

{"title":"Genetic polymorphisms of reproductive hormones and their receptors in assisted reproduction technology for patients with polycystic ovary syndrome.","authors":"Yulia A Koloda, Yulia V Denisova, Natalia M Podzolkova","doi":"10.1515/dmpt-2021-0123","DOIUrl":"https://doi.org/10.1515/dmpt-2021-0123","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies in women of childbearing, which is defined by the accumulation of multiple, small fluid-filled ovarian cysts without the selection of a single dominant follicle. Most PCOS phenotypes are characterized by the absence of spontaneous ovulation, resistance toward ovulation inductors, the production of a large immature oocytes number, and the high prevalence of ovarian hyperstimulation syndrome, resulting in reduced assisted reproductive technologies (ART) programs effectiveness. The review analyses current data about the relationship between polymorphism genotypes of KISS genes, follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-Müllerian hormone (AMH) and their receptors genes, gonadotropin-releasing hormone (GnRH), estrogen, and progesterone receptors genes, the PCOS risk and the features of ovarian response to stimulation during ART cycles. The use of single nucleotide polymorphisms (SNPs) as prognostic markers of ART programs outcomes would provide a personalized approach to the drugs and doses choice for ovarian stimulation and significantly increase the chance of pregnancy.","PeriodicalId":11332,"journal":{"name":"Drug metabolism and personalized therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39682438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1