Diabetic Medicine最新文献

{"title":"Poster abstracts","authors":"","doi":"10.1111/dme.15498","DOIUrl":"https://doi.org/10.1111/dme.15498","url":null,"abstract":"","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 S1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dme.15498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstract reviewers","authors":"","doi":"10.1111/dme.15496","DOIUrl":"https://doi.org/10.1111/dme.15496","url":null,"abstract":"<p>Diabetes UK wishes to express our gratitude to the following abstract reviewers for their support of the Diabetes UK Professional Conference 2025:</p><p>Medha Agrawal</p><p>Shazli Azmi</p><p>Joelle Baynham</p><p>Craig Beall</p><p>Lee-Anne Carson</p><p>Jason Cheung</p><p>Ruth Coleman</p><p>Amy Coulden</p><p>Sarah Davis</p><p>Renuka Dias</p><p>Sarah Dib</p><p>Randa Eltayeb</p><p>Mark Evans</p><p>Bernard Freudenthal</p><p>Jasdeep Giddie</p><p>Alia Gilani</p><p>Mark Green</p><p>Hellena Habte-Asres</p><p>Malak Hamza</p><p>Kate Hardenberg</p><p>Sophie Harris</p><p>Rhiannon Hawkes</p><p>Adrian Heald</p><p>Anwen Jones</p><p>Christina Jones</p><p>Naresh Kanumilli</p><p>Nusrat Kausar</p><p>Catriona Kelly</p><p>Collette Kelly</p><p>Punith Kempegowda</p><p>Benedictine Khor</p><p>Laura Kusinski</p><p>Andrea Lake</p><p>Rama Lakshman</p><p>James Law</p><p>Lala Leelarathna</p><p>Alexandros Liarakos</p><p>Jonathan Maiden</p><p>Aida Martinez-Sanchez</p><p>Rory McCrimmon</p><p>Tracy McCrossan</p><p>Amy McKnight</p><p>Alison McNeilly</p><p>Claire Meek</p><p>Salma Mehar</p><p>Carol Metcalfe</p><p>Nicola Milne</p><p>Emily Moffat</p><p>Ahmad Moolla</p><p>Helen Murphy</p><p>Anuja Natarajan</p><p>Sankalpa Neupane</p><p>Paul Newman</p><p>Sarah Nosib</p><p>Charles Odiase</p><p>Helen Oneil</p><p>Simon O'Neill</p><p>Juliette Palmer</p><p>Mayank Patel</p><p>Reena Patel</p><p>Vinod Patel</p><p>James Pearson</p><p>Tabitha Randell</p><p>Jayne Robbie</p><p>Valmiki Salema</p><p>Priscilla Sarkar</p><p>Shang Shaho</p><p>Marietta Stadler</p><p>Rabbi Swaby</p><p>Rachael Tan</p><p>Hood Thabit</p><p>Aikaterina Tziannou</p><p>Elpida Vounzoulaki</p><p>David Williams</p><p>Komal Zai Rao</p><p>Beenish Zaki</p>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 S1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dme.15496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of a multidisciplinary pilot intervention for parent/caregiver diabetes distress","authors":"E. H. Alkhatib,&nbsp;M. Segawa,&nbsp;A. Danner,&nbsp;H. Kasper,&nbsp;E. Frymark,&nbsp;S. Roberts,&nbsp;L. Clary,&nbsp;R. Streisand,&nbsp;S. Majidi","doi":"10.1111/dme.70006","DOIUrl":"10.1111/dme.70006","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Aims&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Caregiver diabetes distress (DD) consists of negative emotions about a child's T1D diagnosis and has been associated with increased child haemoglobin A1c and family conflict. The primary aim was to identify the feasibility and acceptability of a multidisciplinary pilot intervention for caregiver DD in a diverse academic pediatric diabetes centre. Another primary aim was to determine the percentage of local caregivers with DD. A secondary aim was to determine if the intervention affected caregiver DD. We also explored potential associations between caregiver DD and demographic and diabetes-related characteristics.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Individual interviews with five caregivers with DD (who did not participate in the intervention) were held pre-intervention, in order to gather qualitative, open-ended feedback on prepared educational materials. As a pilot feasibility study, we held 3 monthly, one-hour educational and support group virtual sessions per cohort (6–8 caregivers) on Nutrition, Fear of Hypoglycemia and Parent/Child Collaboration. Sessions were attended by an endocrinologist with leaders from other disciplines including dietician, diabetes educator and/or social worker. Target enrollment was 30 caregivers with DD (PAID-PR/Problem Areas in Diabetes—Parent Revised score of ≥56/100) of children 5–17 years old with T1D for ≥6 months. Data include demographic information, pre-/post- PAID-PR scores, caregivers' session satisfaction ratings and open-ended written feedback. We also examined data from 26 parents of children who did not have elevated scores on DD (the comparison group) and were not invited to attend the intervention sessions.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Caregiver participants were 85% women, ages 42.5 ± 11.3 years; children's characteristics: 38% girls, ages 11.1 ± 2.1 years, 47.6% Black, 33.3% Caucasian, 9.5% Hispanic, 9.6% other, 43% public insurance, mean diabetes duration 4.3 ± 0.18 years and A1c 8.5 ± 0.7% (69.4 mmol/mol). Pre- and post-intervention PAID-PR scores improved from 68 to 51 (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). This was sustained 3 months post-intervention with a mean PAID-PR of 42 (&lt;i&gt;p&lt;/i&gt; &lt; 0.05).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The pilot intervention was feasible, with two-thirds of caregivers attending ≥2 sessions. Reported DD improved over time. Limitations include a small initial sample. Future directions include expanding this study to a larger population and ultimately incorporating a similar support program into the clinical setting as part of routine outpatient diabetes care.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 ","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEKODE-A cloud-based performance feedback model improved DKA care across multiple hospitals in the UK.","authors":"Lakshmi N Rengarajan, Catherine Cooper, Kashish Malhotra, Angelica Sharma, Nevil Philip, Anu Ann Abraham, Ketan Dhatariya, Parth Narendran, Punith Kempegowda","doi":"10.1111/dme.70004","DOIUrl":"https://doi.org/10.1111/dme.70004","url":null,"abstract":"<p><strong>Aim: </strong>A current gap in Diabetes-related ketoacidosis (DKA) research is understanding the factors contributing to variations in care and outcomes between people admitted with DKA. We aimed to create a system to facilitate gathering data on DKA management across multiple centres and identify trends in complications and outcomes associated with DKA.</p><p><strong>Research design and methods: </strong>Between January 2020 and December 2022, we set up a cloud-based Quality improvement project (QIP) that provided regular feedback to 11 hospitals in the United Kingdom (UK).</p><p><strong>Results: </strong>Of the 1977 episodes, we observed an increase in adherence in fluid prescription in hospitals C, D, E, F and G (C- 23% vs. 75% p = <0.001; D- 27% vs. 60%, p = <0.001; E- 17 vs. 79% p = <0.001; F- 16% vs. 57%, p = <0.001; G- 36% vs. 75% p = <0.001). Notable improvements in adherence to glucose monitoring were observed in hospitals B, D, and G (B- 11 vs. 38% p = <0.001; D- 36% vs. 56%, p = 0.05; G- 22% vs. 67% p = <0.001). Although we didn't observe significant changes in complications and outcomes among participating hospitals from the start to the end of the reported period, notable fluctuations were evident across quarters. These variations were relayed to the respective hospitals, underscoring how feedback and interventions could influence the care provided. This initiative also marks the initial move towards establishing and improving data collection practices in acute diabetes.</p><p><strong>Conclusions: </strong>We demonstrate a sustainable QIP that improves adherence to national guidelines in some indicators for DKA care and serves as an early warning system to identify adverse trends.</p>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":" ","pages":"e70004"},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Travel health and people living with type 1 diabetes.","authors":"Steven James, Jayanthi Maniam, Jessica Jones, Olive James, Chloe Tarlton, Judy Craft, Kim C Donaghue, Barnaby Dixson, Maria E Craig","doi":"10.1111/dme.70012","DOIUrl":"https://doi.org/10.1111/dme.70012","url":null,"abstract":"","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":" ","pages":"e70012"},"PeriodicalIF":3.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring healthcare professionals' attitudes to screening for disordered eating in type 1 diabetes","authors":"Katie Fitzgerald,&nbsp;Christina Jones,&nbsp;Helen Partridge,&nbsp;Lindsey Rouse,&nbsp;Rose-Marie Satherley","doi":"10.1111/dme.70003","DOIUrl":"10.1111/dme.70003","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Eating disorders and disordered eating behaviours are prevalent among adults with type 1 diabetes, making early identification essential for improving health outcomes. Although screening tools exist to detect disordered eating in type 1 diabetes (T1DE), their application in clinical practice remains limited. This study investigates healthcare professionals' perspectives and attitudes towards screening for disordered eating in adult diabetes services.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>This qualitative study employed semi-structured interviews with 13 healthcare professionals from adult diabetes services. Purposive sampling was used to ensure a broad range of healthcare professional viewpoints. The interviews centred on their perceptions of screening for disordered eating in type 1 diabetes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Reflexive thematic analysis was used to identify four themes: (1) Uncertainty and Inevitabiltiy of T1DE: ‘A bit of a black hole’, (2) Asking about T1DE: ‘My fear is…am I overstepping the line’, (3) Patient-provider relationships: ‘A backward step’, and (4) Lack of support for T1DE: ‘Where do you go?’. While healthcare professionals recognised the link between diabetes management and disordered eating, they lacked confidence in screening for these challenges due to limited time and the scarcity of specialist disordered eating resources for type 1 diabetes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study highlights the challenges healthcare professionals face in screening for disordered eating in type 1 diabetes. By highlighting the need for enhanced training and clear clinical guidelines, this research suggests pathways to improve healthcare professional confidence in addressing these critical conversations with patients, ultimately supporting better health outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dme.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for the management of diabetes-related ketoacidosis (DKA) have been poorly adopted and implemented, resulting in a lack of improvement in outcomes.","authors":"Angelica Sharma, Lakshmi Rengarajan, Parth Narendran, Ketan Dhatariya, Punith Kempegowda","doi":"10.1111/dme.70010","DOIUrl":"https://doi.org/10.1111/dme.70010","url":null,"abstract":"<p><strong>Aims: </strong>The Joint British Diabetes Society-Inpatient (JBDS-IP) group recommends reducing fixed rate intravenous insulin infusion (FRIII) from 0.1 to 0.05 units/kg/h when blood glucose falls <14 mmol/L to reduce the risk of complications associated with acute management of diabetes-related ketoacidosis. However, whether this change results in real-world improvements is not known.</p><p><strong>Methods: </strong>We performed a retrospective review of DKA admissions between October 2021 and March 2023 across five hospitals in the United Kingdom. We collated data on demographics, biochemical profiles, management interventions, complications, and outcomes.</p><p><strong>Results: </strong>We identified 753 DKA admissions. There was a slow uptake of reduced-rate FRIII, reaching 49.7% over 18 months. In DKA episodes where FRIII rate reduction guidelines were adopted, there was a significant lag (median [IQR] hours) between starting 10% Dextrose and FRIII rate reduction when blood glucose became <14 mmol/L (0.5 (0.1-1.8) vs. 3.2 (0.7-6.5), p = 0.00001). There was no significant reduction in hypoglycaemia (16.5% vs. 13.8%, p = 0.344) in episodes that adopted FRIII reduction. There were no significant differences in the frequency of hypokalaemia, hyperkalaemia, DKA duration, and length of stay between episodes with FRIII rate reduction versus those without FRIII.</p><p><strong>Conclusions: </strong>Our study demonstrates suboptimal adoption of guidelines. Therefore, it was perhaps unsurprising that no favourable effect on the rate of complications or outcomes in DKA episodes with reduced-rate FRIII was demonstrated. In DKA episodes where FRIII rate reduction was adopted, there was a significant delay in adjusting the FRIII when glucose levels were <14 mmol/L. Understanding the barriers and facilitators is vital in creating resources to safely implement guidelines.</p>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":" ","pages":"e70010"},"PeriodicalIF":3.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary interventions for the management of type 2 diabetes mellitus in childhood and adolescence: A systematic review.","authors":"Alisha Bhanot, Katherine Hawton, Rachel Perry, Felix Miller-Molloy, Francesca Blest, Shelley Easter, Julian Hamilton-Shield, Toby Candler","doi":"10.1111/dme.70005","DOIUrl":"https://doi.org/10.1111/dme.70005","url":null,"abstract":"<p><strong>Aims: </strong>Despite the alarming increasing incidence of type 2 diabetes mellitus (T2DM) in children and young People (CYP), and its associated morbidities and poor long-term prognosis, there remains uncertainty in its management. Dietary interventions have been shown to be effective in adults with T2DM, but little is known about their effectiveness in CYP. The aim of this systematic review is to provide up-to-date evidence regarding dietary interventions for T2DM in childhood and adolescence.</p><p><strong>Methods: </strong>Five databases Embase, MEDLINE, CENTRAL, Web of Science and CINAHL were searched from January 2000 to May 2023 for all studies involving dietary interventions in CYP under 19 years with T2DM. The primary outcome was glycaemic control as measured by HbA1c.</p><p><strong>Results: </strong>Of 8352 search results, five papers met inclusion criteria. No randomised controlled trials were identified. Two interventional studies (n = 28) found very low energy diets (VLED) were associated with reduced HbA1c (16 mmol/mol (3.6%) reduction after 8 weeks), decreased requirement for pharmacotherapy and weight loss. However, benefits to HbA1c were not sustained over 2 years. From the observational studies, the most frequent self-reported dietary strategies were limiting sweets and increasing fruit/vegetable intake, but efficacy was limited. Limiting fat intake was associated with improved HbA1c in women.</p><p><strong>Conclusions: </strong>There is limited evidence and a lack of robust clinical trials to support the effectiveness of dietary interventions for CYP-onset T2DM. With evidence of benefit in adulthood and encouraging initial results in the young, it is imperative that fully powered randomised trials with longer follow-up are undertaken to determine efficacy.</p>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":" ","pages":"e70005"},"PeriodicalIF":3.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening for disordered eating in adolescents with Type 1 diabetes: A comparison of Diabetes Eating Problem Survey Revised (DEPS-R) and Youth Eating Disorder Examination Questionnaire (YEDE-Q) with item-level analysis","authors":"Caroline B. Abild,&nbsp;Loa Clausen,&nbsp;Line Wisting,&nbsp;Jens Meldgaard Bruun,&nbsp;Kurt Kristensen,&nbsp;Rene Klinkby Støving,&nbsp;Esben Thyssen Vestergaard","doi":"10.1111/dme.15521","DOIUrl":"10.1111/dme.15521","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>People with type 1 diabetes (T1D) face an increased risk of disordered eating (DE) and eating disorders (ED), associated with glucose levels outside target range and an increased risk of complications. The aim of this study was to investigate the type and severity of DE and assess the item accuracy of the Diabetes Eating Problem Survey Revised (DEPS-R) compared to the Youth Eating Disorder Examination Questionnaire (YEDE-Q) in the screening of DE among adolescents with T1D.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Cross-sectional online survey including DEPS-R, YEDE-Q and medical records. Item to total analysis with correlations, and group comparisons were performed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 131 adolescents between 11 and 19 (mean age 15.3 ± 2.2) years completed both DEPS-R and YEDE-Q, identifying 21.4% and 22.9% with DE, respectively. Significantly more females (&gt;30%) than males (≈5%) were identified in both measures. More than 50% reported insulin restriction in DEPS-R vs. 1.5% in YEDE-Q. YEDE-Q yielded 32% with weight controlling behaviours, however, half of these were unidentified by DEPS-R. Item-total analysis of DEPS-R showed that item 10 had small correlations to both total score and clinical variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study confirms the clinical relevance of DEPS-R, including associations between DE and female sex, older age, and glucose levels outside target range, however, it may not accurately capture distinct ED risk behaviours, such as excessive exercise or shape and weight related insulin restriction. The item-total analysis shows that the relevance of item 10 should be further investigated.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dme.15521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock-pretreated bone marrow mesenchymal stem cells accelerate wound healing in a diabetic foot ulcer rat model","authors":"Xi Lin,&nbsp;Qi Lin","doi":"10.1111/dme.15507","DOIUrl":"10.1111/dme.15507","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Diabetic foot ulcers (DFUs) are the severe chronic complications of diabetes, amputation is required when ulcers cause severe loss of tissue or evoke a life-threatening infection. Mesenchymal stem cells (MSCs) have shown a good effect in helping DFU healing, though the efficiency needs to be improved. This study aimed to investigate the effects of heat shock pretreatment on the improvement of the therapeutic effects of MSCs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Primary rat bone marrow MSCs (BMSCs) were isolated and stimulated with heat shock pretreatment and then tested on a DFU rat model. Alkaline phosphatase, Alizarin Red S, and Oil Red O were stained to check the osteogenic differentiation ability of heat shock-pretreated BMSCs. The effect of heat shock pretreatment on the inflammatory response of macrophages was studied with the lipopolysaccharides stimulation model on a mouse macrophage cell line RAW264.7. The impact of heat shock-pretreated BMSCs on dermal fibroblasts was also checked. Last, heat shock-pretreated BMSCs were tested on a DFU rat model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Heat shock-pretreated BMSCs were characterized by the expression of CD105 and CD44. Heat shock pre-stimulation did not affect cell viability when cultured up to 96 h. Heat shock pre-stimulated BMSCs inhibited the inflammatory response by reducing the pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and enhancing the anti-inflammatory cytokine production (IL-10) (at least all <i>p</i> &lt; 0.01), as well as increasing the ratio of M2 polarization macrophages to M1 polarization in vitro (<i>p</i> &lt; 0.001). Heat shock pre-stimulated BMSCs enhanced the growth and migration of dermal fibroblasts in vitro (<i>p</i> &lt; 0.001). Heat shock-BMSCs promoted the M2 polarization level of macrophages in wound tissues in a DFU rat model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Heat shock pretreatment could enhance the therapeutic effect of BMSCs on wound healing in a DFU rat model.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}