DNA and cell biology最新文献

{"title":"The Novel Circular RNA circTRIO Silence Inhibits the Progression of Laryngeal Squamous Cell Carcinoma.","authors":"Jing Wang,&nbsp;Shujin He,&nbsp;Dingding Wang,&nbsp;Yanlei Jing,&nbsp;Wenping Shen,&nbsp;Anqi Huang,&nbsp;Zhe Zhou,&nbsp;Honggang Liu","doi":"10.1089/dna.2023.0043","DOIUrl":"https://doi.org/10.1089/dna.2023.0043","url":null,"abstract":"<p><p>Laryngeal squamous cell carcinoma (LSCC) is the most common and prevalent malignant tumor in head and neck squamous cell carcinoma. Recent studies have shown that circular RNAs (circRNAs) play a vital role in cancer development, but their specific role in the tumorigenesis and development of LSCC remains unclear. We selected five pairs of LSCC tumor tissues and paracancerous tissues for RNA sequencing. Reverse transcription-quantitative PCR (RT-qPCR), Sanger sequencing, and fluorescence <i>in situ</i> hybridization were utilized to study the expression, localization, and clinical significance of circTRIO in LSCC tissues, and TU212 and TU686 cell lines. Furthermore, cell counting Kit-8, colony-forming assay, Transwell, and flow cytometry assays were assessed to illustrate the crucial role played by the circTRIO in proliferation, colony-forming ability, migration, and apoptosis in LSCC cells. Finally, the molecule's role as a microRNA (miRNA) sponge was analyzed. In the results, we screened out a promising upregulated novel circRNA-circTRIO in LSCC tumor tissues compared with paracancerous tissues using RNA sequencing. Then, we used qPCR to evaluate the expression of circTRIO in 20 additional paired LSCC tissues and two cells, and the results showed that circTRIO was highly expressed in LSCC and that this high expression was closely related to the malignant progression of LSCC. Furthermore, we examined circTRIO expression in the Gene Expression Omnibus data sets GSE142083 and GSE27020, and circTRIO expression was considerably higher in tumor tissues compared with the adjacent tissues. Kaplan-Meier survival analysis showed that the expression of circTRIO was associated with worse disease-free survival. The Gene Set Enrichment Analysis biological pathway evaluation results demonstrated that circTRIO was mainly enriched in cancer pathways. Moreover, we confirmed that silencing circTRIOs can help to significantly inhibit LSCC cell proliferation and migration while triggering apoptosis. Upregulated circTRIO expression levels may play an important role in the tumorigenesis and development of LSCC.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9791204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Dynamics of Podocyte in Various International Society of Nephrology/Renal Pathology Society Class Lupus Nephritis Patients.","authors":"Qijiao Wei,&nbsp;Qing Yan,&nbsp;Shengli Zhang,&nbsp;Fei Gao,&nbsp;Zhihan Chen,&nbsp;He Lin,&nbsp;Li Sun","doi":"10.1089/dna.2022.0636","DOIUrl":"https://doi.org/10.1089/dna.2022.0636","url":null,"abstract":"<p><p>Morphological changes of podocyte mitochondria are observed in patients with mitochondrial cytopathy and nephrotic syndrome. However, whether mitochondrial dynamics involved in podocyte in lupus nephritis (LN) is still not clear. This study aims to investigate the associations between mitochondrial morphology and podocyte lesions and laboratory and pathological features in LN. The foot process width (FPW) and mitochondrial morphology were observed through electron microscope. Then the associations between mitochondrial morphology and podocyte lesions and laboratory features were explored in various International Society of Nephrology/Renal Pathology Society class LN patients. Foot process effacement and excessive mitochondria fission in podocyte were observed and proteinuria was positively correlated with FPW. Mitochondria area, circumference, and aspect ratio were negatively correlated with BUN, and 24h-UTP positively correlated with Alb. At the same time, Alb was negatively correlated with form factor. FPW, form factor, surface density, and numerical density on area were positively correlated with 24h-UTP. Excessive mitochondrial fission is associated with podocyte damage and proteinuria, whereas the mechanism still needs to be explored.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Analysis Reveals the Vital Role of AKR1B1 in Immune Infiltration and Clinical Outcomes of Gastric Cancer.","authors":"Zhiyue Zhao,&nbsp;Zhibin Hao,&nbsp;Zheng Zhang,&nbsp;Xianbao Zhan","doi":"10.1089/dna.2022.0644","DOIUrl":"https://doi.org/10.1089/dna.2022.0644","url":null,"abstract":"<p><p>Infiltrated immune cells are an important constitute of tumor microenvironment, which exert complex effects on gastric cancer (GC) pathogenesis and progression. By using weighted gene co-expression network analysis, integrating the data from The Cancer Genome Atlas-stomach adenocarcinoma and GSE62254, we identify Aldo-Keto Reductase Family 1 Member B (AKR1B1) as a hub gene for immune regulation in GC. Notably, AKR1B1 is associated with higher immune infiltration and worse histologic grade of GC. In addition, AKR1B1 is an independent factor for predicting the survival rate of GC patients. <i>In vitro</i> experiments further demonstrated that AKR1B1-overexpressed THP-1-derived macrophages promoted the proliferation and migration of GC cells. Taken together, AKR1B1 plays an important role in GC progression by regulating immune microenvironment, which could be a biomarker for predicting GC prognosis as well as a potential therapeutic target for GC treatment.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9787047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium Nanoparticles Show Anticancer Activity Through Regulation of HIF-1α and HIF-2α Under Hypoxic Condition in Liver Cancer Cells.","authors":"Sancharan Acharya,&nbsp;Subramaniyam Nithyananthan,&nbsp;Chinnasamy Thirunavukkarasu","doi":"10.1089/dna.2023.0099","DOIUrl":"https://doi.org/10.1089/dna.2023.0099","url":null,"abstract":"<p><p>Tumor microenvironment has significant influence in therapeutic response and clinical outcome. Combination therapy is more effective in cancer treatment compared with monotherapy. Any chemical or drug that targets tumor microenvironment pathway, will be a boon to combination cancer chemotherapy. Combination therapy through micronutrient may have added advantage in clinical applications. Selenium (Se) is an essential micronutrient; Se in the form of Se nanoparticles (SeNPs) show efficient anticancer properties and may have the potential to target tumor niche such as hypoxic environment. The aim of this study was to find out the anticancer effect of SeNPs on cell line HepG2 under hypoxic condition and also to evaluate their effect on the translocation of hypoxia-inducible factors (HIFs) from cytoplasm to nucleus that help the cells to survive under hypoxic condition. It was found that the SeNPs induce HepG2 cell death in normoxic and hypoxic conditions, however, hypoxic condition showed higher LD₅₀. SeNP concentration is directly proportional to cell death in both the conditions. Furthermore, intracellular accumulation of Se is not affected by hypoxia. SeNP-induced HepG2 cell death is due to increased DNA damage, nuclear condensation, and mitochondrial membrane potential disturbance. Furthermore, SeNPs were also found to decrease the translocation of HIFs from cytosol to the nucleus. After analyzing the results, it is concluded that SeNP treatment disturbs tumor niche through the inhibition of HIFs' translocation from cytosol to nucleus. SeNPs in synergy with primary drug, such as doxorubicin (DOX), may enhance the anticancer efficacy of DOX through regulation of HIFs, warranting further research.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9791203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>FTO</i> Promotes the Stemness of Gastric Cancer Cells.","authors":"Mengqing Li,&nbsp;Xuan Wu,&nbsp;Guan Li,&nbsp;Guoqing Lv,&nbsp;Shubin Wang","doi":"10.1089/dna.2023.0074","DOIUrl":"https://doi.org/10.1089/dna.2023.0074","url":null,"abstract":"<p><p>The full name of the FTO gene is fat mass and obesity-associated gene. In recent years, it has also been found that FTO is involved in m6A demethylation and regulates the progression of multiple cancers, including gastric cancer. The cancer stem cell theory argues that cancer stem cells are key factors in cancer metastasis, and inhibiting the expression of stemness genes is a good method to inhibit metastasis of gastric cancer. Currently, the role of the FTO gene in regulating stemness of gastric cancer cells is still unclear. By analyzing public databases, it was discovered that FTO gene expression was increased in gastric cancer, and high expression of FTO was associated with poor prognosis of patients with gastric cancer. After gastric cancer stem cells were isolated, it was found that FTO protein expression was increased in gastric cancer stem cells; stemness of gastric cancer cells was reduced after the FTO gene knockdown; subcutaneous tumors of nude mice were smaller than those of the control group after FTO knockdown; and stemness of gastric cancer cells was enhanced after FTO was overexpressed by plasmid. By reviewing additional literature and experimental validation, we found that SOX2 may be the factor by which FTO promotes the stemness of gastric cancer cells. Therefore, it was concluded that FTO could promote the stemness of gastric cancer cells, and targeting FTO may be a potential therapeutic approach for patients with metastatic gastric cancer. CTR number: TOP-IACUC-2021-0123.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for <i>DNA and Cell Biology</i>.","authors":"Carolin M Lieber","doi":"10.1089/dna.2023.29023.rfs2022","DOIUrl":"https://doi.org/10.1089/dna.2023.29023.rfs2022","url":null,"abstract":"","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA <i>MIR31HG</i> Regulates Proliferation and Migration by Targeting Matrix Gla Protein in Nonsyndromic Cleft Lip With or Without Cleft Palate.","authors":"Xiaofeng Li, Xinze Xu, Luwei Liu, Yu Tian, Yue Gao, Guirong Zhu, Shu Lou, Weijie Zhong, Dandan Li, Yongchu Pan","doi":"10.1089/dna.2022.0657","DOIUrl":"10.1089/dna.2022.0657","url":null,"abstract":"<p><p>Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is a common craniofacial birth defect with complex etiologies. Recently, the dysregulation of long noncoding RNAs (lncRNAs) has been implicated in many developmental diseases, including NSCL/P. However, the functions and mechanisms of lncRNAs in NSCL/P have not been fully elucidated. In this study, we found that lncRNA <i>MIR31HG</i> in NSCL/P patients was significantly downregulated than that in healthy individuals (GSE42589, GSE183527). In addition, single nucleotide polymorphism rs58751040 in <i>MIR31HG</i> was nominally associated with NSCL/P susceptibility (odds ratio: 1.29, 95% confidence interval: 1.03-1.54, <i>p</i> = 4.93 × 10^-2) through a case-control study (504 NSCL/P cases and 455 controls). Luciferase activity assay showed that the C allele of rs58751040 revealed a decreased transcription activity of <i>MIR31HG</i> than the G allele. Moreover, knockdown of <i>MIR31HG</i> promoted cell proliferation and migration in human oral keratinocytes and human embryonic palate mesenchyme. Bioinformatic analysis and cellular studies suggested that <i>MIR31HG</i> may confer susceptibility to risk of NSCL/P through matrix Gla protein (<i>MGP</i>) signaling. In summary, we identified a novel lncRNA involved in the development of NSCL/P.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9800718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of <i>circIGF1R</i> Suppresses Cardiomyocytes Proliferation by Sponging <i>miR-362-5p</i>.","authors":"Jun-Hui Zeng,&nbsp;Hong-Ji Li,&nbsp;Kun Liu,&nbsp;Chi-Qian Liang,&nbsp;Hai-Yan Wu,&nbsp;Wu-Yun Chen,&nbsp;Ming-Hui Tang,&nbsp;Wan-Ling Zhao,&nbsp;Dong-Qing Cai,&nbsp;Xu-Feng Qi","doi":"10.1089/dna.2022.0590","DOIUrl":"https://doi.org/10.1089/dna.2022.0590","url":null,"abstract":"<p><p>Circular RNAs (circRNAs) are generally formed by the back-splicing of precursor mRNA. Increasing evidence implicates the important role of circRNAs in cardiovascular diseases. However, the role of circ-insulin-like growth factor 1 receptor (<i>circIGF1R</i>) in cardiomyocyte (CM) proliferation remains unclear. Here, we investigated the potential role of the <i>circIGF1R</i> in the proliferation of CMs. We found that <i>circIGF1R</i> expression in heart tissues and primary CMs from adult mice was significantly lower than that in neonatal mice at postnatal 1 day (p1). Increased <i>circIGF1R</i> expression was detected in the injured neonatal heart at 0.5 and 1 days post-resection. <i>circIGF1R</i> knockdown significantly decreased the proliferation of primary CMs. Combined prediction software, luciferase reporter gene analysis, and quantitative real time-PCR (qPCR) revealed that <i>circIGF1R</i> interacted with <i>miR-362-5p</i>. A significant increase in <i>miR-362-5p</i> expression was detected in the adult heart compared with that in the neonatal heart. Further, heart injury significantly decreased the expression of <i>miR-362-5p</i> in neonatal mice. Treatment with <i>miR-362-5p</i> mimics significantly suppressed the proliferation of primary CMs, whereas knockdown of <i>miR-362-5p</i> promoted the CMs proliferation. Meanwhile, <i>miR-362-5p</i> silencing can rescue the proliferation inhibition of CMs induced by <i>circIGF1R</i> knockdown. Target prediction and qPCR validation revealed that <i>miR-362-5p</i> significantly inhibited the expression of <i>Phf3</i> in primary CMs. In addition, decreased <i>Phf3</i> expression was detected in adult hearts compared with neonatal hearts. Consistently, increased <i>Phf3</i> expression was detected in injured neonatal hearts compared with that in sham hearts. Knockdown of <i>Phf3</i> markedly repressed CMs proliferation. Taken together, these findings suggest that <i>circIGF1R</i> might contribute to cardiomyocyte proliferation by promoting <i>Pfh3</i> expression by sponging <i>miR-362-5p</i> and provide an important experimental basis for the regulation of heart regeneration.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9799753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the Mitogen-Activated Protein Kinase Pathway on the Erastin-Induced Ferroptosis of Molt-4 Cells.","authors":"Nana Liu,&nbsp;Ge Liu,&nbsp;Haihong Jiang,&nbsp;Jing Yu,&nbsp;Yunqin Jin,&nbsp;Hong Wang","doi":"10.1089/dna.2022.0661","DOIUrl":"https://doi.org/10.1089/dna.2022.0661","url":null,"abstract":"<p><p>The role of ferroptosis in human acute lymphoblastic leukemia and its possible molecular mechanisms of action are still unknown. In this study, harvested Molt-4 cells were exposed to different concentrations of erastin, and their proliferation capacity was tested by using the cell counting kit-8 assay. Lipid peroxidation levels were detected through flow cytometry. Mitochondrial alterations were observed through transmission electron microscopy. The expression levels of SLC7A11, glutathione peroxidase 4 (GPX4), and mitogen-activated protein kinase (MAPK) were detected by using quantitative real-time PCR and Western blot analysis. This study found that erastin inhibited the growth of Molt-4 cells. This inhibitory effect could be partially reversed by the ferroptosis inhibitor Ferrostatin-1 and the p38 MAPK inhibitor. The mitochondria of Molt-4 cells treated with erastin shortened and condensed. Compared with those in the control group, the levels of reactive oxygen species and malondialdehyde had increased, whereas the levels of glutathione had decreased in the treatment group. The treatment of Molt-4 cells with erastin decreased the levels of SLC7A11 and GPX4 mRNA and increased the expression levels of p38 MAPK, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase. These findings suggested that erastin caused the ferroptosis of Molt-4 cells. This process may be correlated with the inhibition of the cystine/glutamate antiporter system and GPX4 and the activation of p38 MAPK and ERK1/2.</p>","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9575486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding Opportunities in Viral Oncolysis.","authors":"Janaina Fernandes","doi":"10.1089/dna.2023.0129.editorial","DOIUrl":"https://doi.org/10.1089/dna.2023.0129.editorial","url":null,"abstract":"","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10224897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}