Developments in biologicals最新文献_第9页

Proteomic dissection of viral pathogenesis. 病毒发病机制的蛋白质组学解剖。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317143

H C Liu, J Hicks, D Yoo

引用次数: 10

The future impact of animal genomics in animal health research. 动物基因组学在动物健康研究中的未来影响。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317139

M-H Pinard-Van Der Laan, C G Gay

引用次数: 1

The rabies situation in Western Europe. 西欧的狂犬病情况。

Developments in biologicals Pub Date : 2008-01-01

A I Wandeler

引用次数: 0

Ten-day observation of live rabies suspected dogs. 对怀疑患有狂犬病的活犬进行为期十天的观察。

Developments in biologicals Pub Date : 2008-01-01

V Tepsumethanon, H Wilde, V Sitprija

引用次数: 0

Experimental infection of foxes with European bat lyssaviruses type-1 and -2. 1型和2型欧洲蝙蝠溶血病毒对狐狸的实验性感染。

Developments in biologicals Pub Date : 2008-01-01

E Picard-Meyer, S M Brookes, J Barrat, E Litaize, C Patron, M Biarnais, D M Healy, L Johnson, A R Fooks, F Cliquet

{"title":"Experimental infection of foxes with European bat lyssaviruses type-1 and -2.","authors":"E Picard-Meyer, S M Brookes, J Barrat, E Litaize, C Patron, M Biarnais, D M Healy, L Johnson, A R Fooks, F Cliquet","doi":"","DOIUrl":"","url":null,"abstract":"Experimental studies have been undertaken to assess the susceptibility of silver foxes to bat variants of rabies virus, namely European Bat Lyssaviruses (EBLVs). Both EBLV-1 and EBLV-2 have been isolated in European bats since 1954, in Eptesicus serotinus and Myotis species, respectively. Since 2000, the number of reported cases has increased largely due to the improvement of the surveillance of bat rabies virus throughout Europe. Although over >800 EBLVs cases have been reported in bats in Europe, EBLV-1 and -2 viruses are rarely reported to infect humans and terrestrial animals. The study presented here shows that the sensitivity of silver foxes is low when infected with EBLVs via the intramuscular route; in contrast all animals infected via intracranial inoculation succumbed to the experimental challenge. The mortality rate was 100% for both EBLV-1 (approximately 4.5 log) and EBLV-2 (approximately 3.0 log). This data suggests that the susceptibility of foxes to EBLV-1 and EBLV-2 is low and that the transmission (spillover) and adaptation of EBLVs from a bat to a fox may be theoretically possible but unlikely.","PeriodicalId":11190,"journal":{"name":"Developments in biologicals","volume":"131 ","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27541548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finnish-Russian programme for the control of rabies (2005-2010). 芬兰-俄罗斯狂犬病控制规划(2005-2010年)。

Developments in biologicals Pub Date : 2008-01-01

A E Metlin, S S Rybakov, K N Gruzdev, V V Mikhalishin, A Huovilainen, E Neuvonen

引用次数: 0

The use of RNAi and transgenics to develop viral disease resistant livestock. 利用RNAi和转基因技术培育抗病毒家畜。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317188

T G Wise, D S Schafer, J W Lowenthal, T J Doran

{"title":"The use of RNAi and transgenics to develop viral disease resistant livestock.","authors":"T G Wise, D S Schafer, J W Lowenthal, T J Doran","doi":"10.1159/000317188","DOIUrl":"https://doi.org/10.1159/000317188","url":null,"abstract":"The possibility of genetically engineering poultry to make them resistant to avian influenza is attracting attention and has now become a real possibility with improved methods for genetic modification and the emergence of RNAi as an antiviral strategy. In order to test this possibility, we have generated transgenic mice that express RNAi molecules targeting a conserved region of the influenza A NP gene and are testing these mice for resistance to influenza infection. Transgenes were initially developed that express short hairpin RNAs (shRNAs) targeting multiple influenza A viral genes. The shRNAs were tested for inhibition of H1N1 PR8 virus in vitro. Two potent shRNAs that target the NP and PA genes were chosen for lentiviral mediated generation of transgenic mice. Transgenic founders for the NP shRNA construct and also a negative control shRNAtargeting EGFP were generated. The constitutive expression of the shRNA molecules in a range of tissue types including lung, was confirmed and so far stable transmission of the RNAi transgenes from the F0 to F3 generation has been observed. Resistance to influenza infection in these transgenic mice is now being confirmed.","PeriodicalId":11190,"journal":{"name":"Developments in biologicals","volume":"132 ","pages":"377-382"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27695984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Bovine viral diarrhoea virus infection alters global transcription profiles in bovine endothelial cells. 牛病毒性腹泻病毒感染改变了牛内皮细胞的全局转录谱。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317148

J D Neill, J F Ridpath, A Lange, R L Zuerner

{"title":"Bovine viral diarrhoea virus infection alters global transcription profiles in bovine endothelial cells.","authors":"J D Neill, J F Ridpath, A Lange, R L Zuerner","doi":"10.1159/000317148","DOIUrl":"https://doi.org/10.1159/000317148","url":null,"abstract":"Bovine viral diarrhoea viruses (BVDV) are significant pathogens of cattle worldwide. These viruses exist in both non-cytopathic and cytopathic biotypes. Non-cytopathic BVDV can establish persistent lifelong infections in cattle and are a frequent contaminant of biological reagents such as cell cultures and foetal bovine serum. We identified commercially available bovine aortic endothelial cells (BAECs) contaminated with BVDV. In this study, to determine if BVDV alters endothelial gene transcription patterns, serial analysis of gene expression (SAGE) was used to compare gene expression profiles from uninfected and BVDV contaminated BAEC. SAGE is an open ended, quantitative method for characterizing global patterns of transcription. Comparison of expression profiles of BVDV-contaminated and noninfected cells revealed significant increases in the transcription of many genes including P-selectin, tryptophan tRNA synthetase and prostaglandin D2 synthase. These changes were validated by real-time PCR. Additionally, real-time PCR demonstrated that the response to LPS and dsRNA by contaminated cells, as well as cells acutely infected with noncytopathic BVDV, is altered. The altered response may be through the high level of expression of A20 and inhibition of activation of NF-kappaB. BAECs are commonly used as a model to study endothelial cell function in many different systems. As shown here, transcriptional and probable protein changes resulting from BVDV infection significantly alter cellular responses and may have a profound impact on experimental outcome. Transcriptomic analysis provided the initial clues leading to the characterization of this altered function.","PeriodicalId":11190,"journal":{"name":"Developments in biologicals","volume":"132 ","pages":"93-98"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27696338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Porcine genome-wide gene expression in response to tetanus toxoid vaccine. 猪对破伤风类毒素疫苗应答的全基因组基因表达。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317159

S Ponsuksili, E Murani, K Wimmers

{"title":"Porcine genome-wide gene expression in response to tetanus toxoid vaccine.","authors":"S Ponsuksili, E Murani, K Wimmers","doi":"10.1159/000317159","DOIUrl":"https://doi.org/10.1159/000317159","url":null,"abstract":"The very early in vivo response to immune stimuli was studied using tetanus toxoid as a model antigen known to induce Th1 and Th2 responses. Eighteen weaning piglets were vaccinated subcutaneously with tetanus toxoid. Leukocyte RNAs were isolated from samplings before and 2, 4, 8, and 24 hours after vaccination. After competitive hybridization of a 13297 porcine 70-mer oligo microarray (Qiagen-Operon NRSP8), subsequent image analysis and normalization, the data was analysed using analyses of variance (ANOVA) to identify genes regulated due to vaccination (ANOVA, p < or = 0.05; corresponding to false discovery q < or = 0.12). Of 8240 probes representing genes expressed in leukocytes, 1289 genes showed differential expression. Results were exemplarily confirmed by real-time PCR. Holistic expression data was clustered to six prominent groups of genes with similar changes in expression patterns using a k-means algorithm. To get more insight into functional and structural components and impact of the genes represented in each cluster, the EASE score was used to identify Gene Ontology functional categories. The results showed that in vivo significant changes of expression profiles of peripheral blood mononuclear cells (PBMCs) occurred very early after immune stimuli. These alterations concerned genes of pathways related to immune response as well as other metabolic and regulatory pathways including ATP/energy metabolism, transcription, structural molecule activity, biosynthesis, and metabolism. The analysis reveals new functional candidate genes for traits related to immune responsiveness and also provides new insight into the interaction of immune response, and metabolic and endocrine status. This will facilitate a better understanding of the relationship between immune and performance traits.","PeriodicalId":11190,"journal":{"name":"Developments in biologicals","volume":"132 ","pages":"185-195"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27696700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Predicting genetic merit for mastitis and fertility in dairy cattle using genome wide selection and high density SNP screens. 利用基因组广泛选择和高密度SNP筛选预测奶牛乳腺炎和生育力的遗传优势。

Developments in biologicals Pub Date : 2008-01-01 DOI: 10.1159/000317163

H W Raadsma, G Moser, R E Crump, M S Khatkar, K R Zenger, J A L Cavanagh, R J Hawken, M Hobbs, W Barris, J Solkner, F W Nicholas, B Tier

{"title":"Predicting genetic merit for mastitis and fertility in dairy cattle using genome wide selection and high density SNP screens.","authors":"H W Raadsma, G Moser, R E Crump, M S Khatkar, K R Zenger, J A L Cavanagh, R J Hawken, M Hobbs, W Barris, J Solkner, F W Nicholas, B Tier","doi":"10.1159/000317163","DOIUrl":"https://doi.org/10.1159/000317163","url":null,"abstract":"Two novel methods for genome wide selection (GWS) were examined for predicting the genetic merit of animals using SNP information alone. A panel of 1,546 dairy bulls with reliable EBVs was genotyped for 15,380 SNPs that spanned the whole bovine genome. Two complexity reduction methods were used, partial least squares (PLS) and regression using a genetic algorithm (GAR), to find optimal solutions of EBVs against SNP information. Extensive internal cross-validation was used tofind the best predictive models followed by external validation (without direct use of the pedigree or SNP location). Both PLS and GAR provided both accurate fit to the training data set for somatic cell count (SCC) (max r = 0.83) and fertility (max r = 0.88) and showed an accuracy of prediction of r = 0.47 for SCC, and r = 0.72 for fertility. This is the first empirical demonstration that genome wide selection can account for a very high proportion of additive genetic variation in fitness traits whilst exploiting only a small percentage of available SNP information, without use of pedigree or QTL mapping. PLS was computationally more efficient than GAR.","PeriodicalId":11190,"journal":{"name":"Developments in biologicals","volume":"132 ","pages":"219-223"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27696704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14