2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC)最新文献_第4页

Role of fluid shear stress on E-cadherin dynamics and cytoskeletal stresses 流体剪切应力对e -钙粘蛋白动力学和细胞骨架应力的影响

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117053

Deepika Verma, N. Ye, S. Hua

{"title":"Role of fluid shear stress on E-cadherin dynamics and cytoskeletal stresses","authors":"Deepika Verma, N. Ye, S. Hua","doi":"10.1109/NEBEC.2015.7117053","DOIUrl":"https://doi.org/10.1109/NEBEC.2015.7117053","url":null,"abstract":"Adherens junctions (AJs) play an important role in cell-cell mechanical coupling and in maintaining tissue organization. Under chronic flow, epithelial cells adapt by strengthening cell-cell junctions in order to maintain the integrity of epithelia. How forces due to fluid flow are transmitted to E-cadherin and cause the dynamic remodeling of AJs is unclear. In this study we simultaneously measured AJ dynamics and cytoskeletal stresses and reorganization in MDCK cells under flow. The cells were co-expressed with fluorescently labelled E-cadherin and stress sensitive α-actinin FRET sensor for measurements. A fluid shear stress of 0.7dyn/cm2 for 3 hours caused an increase in expression of E-cadherin, formation of new AJs and strengthening of pre-existing AJs leading to a continuous distribution of cell-cell junctions. This increase in cell-cell junctions is correlated with the formation of strong peripheral actin ring due to cytoskeleton reorganization. The average cytoskeletal stress at adherens junctions shows multi-phase changes under flow, which closely links to the dynamic changes of AJs. These results provide the direct evidence that the shear stress induced cytoskeletal forces play an important role on cell-cell adhesions.","PeriodicalId":111398,"journal":{"name":"2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC)","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122450150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Quantification of entrapped model protein released during electrospun nanofiber degradation 电纺丝纳米纤维降解过程中捕获模型蛋白的定量分析

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117086

K. Cummins, K. L. Lee, J. A. Cooper

引用次数: 3

In vitro dynamic simulation of elbow motion 肘关节运动的体外动态模拟

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117109

M. Lazar, A. Lurski, S. Ghafurian, L. Chen, L. Uko, V. Tan, K. Li

引用次数: 0

Calcaneus stress fractures: A finite element analysis 跟骨应力性骨折:有限元分析

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117075

A. M. J. Aljabran, D. Piovesan, R. Bortoletto

引用次数: 1

Frozen naphthalocyanine micelles for intestinal imaging 冷冻萘菁胶束用于肠道成像

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117210

Yumiao Zhang, Mansik Jeon, L. Rich, H. Hong, J. Geng, Yin Zhang, Sixiang Shi, T. Barnhart, P. Alexandridis, J. Huizinga, M. Seshadri, W. Cai, C. Kim, J. Lovell

引用次数: 0

Orthogonally tunable solid-state nanopore modifications for improved biosensing 用于改善生物传感的正交可调固态纳米孔修饰

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117050

J. S. Hersey, A. Squires, A. Meller, M. Grinstaff

{"title":"Orthogonally tunable solid-state nanopore modifications for improved biosensing","authors":"J. S. Hersey, A. Squires, A. Meller, M. Grinstaff","doi":"10.1109/NEBEC.2015.7117050","DOIUrl":"https://doi.org/10.1109/NEBEC.2015.7117050","url":null,"abstract":"Advances in biosensor sensitivity, specificity, and accessibility are required for the development of next generation diagnostic tools. Solid-state nanopores consisting of sub-10 nm in diameter pores drilled into an insulating material show promise as single-molecule biosensors for detecting both nucleic acid and protein targets. However, this technology is limited by rapid analyte translocation speeds and complex and/or inconsistent device assemblies. To address these limitations, we have developed two orthogonally tunable solid-state nanopore modifications which slow nucleic acid translocation speeds and streamline device assembly and manipulation through the use of a 3-dimensional polymeric nanofiber mesh (NFM) coating and the development of a novel microfluidic device, respectively. A range of translocation speeds from 1x to >100x slower than a bare nanopore were achieved by tuning the chemical composition of the NFM coating. In addition, a microfluidic device was designed to streamline nanopore assembly enabling facile integration of both sample purification protocols and single-molecule detection using optical and electronic readouts simultaneously.","PeriodicalId":111398,"journal":{"name":"2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129092338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofabrication and 3D localization of multilayered cellular constructs using Laser Direct-Write and Mesoscopic Fluorescent Molecular Tomography 使用激光直写和介观荧光分子层析成像的多层细胞结构的生物制造和三维定位

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117130

B. Tricomi, Mehmet S. Ozturk, X. Intes, D. Corr

引用次数: 3

Saving navy SEALS: Pressure-activated system for personal flotation device 拯救海豹突击队:个人漂浮装置的压力激活系统

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117227

N. Miller, J. M. Adams

{"title":"Saving navy SEALS: Pressure-activated system for personal flotation device","authors":"N. Miller, J. M. Adams","doi":"10.1109/NEBEC.2015.7117227","DOIUrl":"https://doi.org/10.1109/NEBEC.2015.7117227","url":null,"abstract":"Approximately 2000 operators in the SEAL Teams use a personal flotation device (PFD). While it is a necessary safety measure, the current PFDs in use often deploy prematurely causing the operator to then discard the device. We created a system with changeable modes, allowing the user to retain a fully functional vest with an activation system that is not subject to premature deployment. Implementation of this will reduce the danger for each operator and save lives. Our system is composed of a sensor suite, microcontroller, and actuator. The system has two user chosen modes: automatic and manual. We performed system tests to ensure the system is reliable and validated for use in special operations. Two of these tests included a software functionality test (A) and a valve response test (B). Results for (A) were LED ON for deployment conditions and LED OFF for non-deployment conditions. Results for (B) were a positive result (actuator activated and filled bladder correctly) for each test. The final result was an automatic PFD system that is viable for use in special operations. This will increase the number of lives saved by ensuring that operators can retain their PFDs without premature deployment as well as ensuring that an unconscious victim in the water will be lifted to safety by their automatic PFD.","PeriodicalId":111398,"journal":{"name":"2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131928986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization and evaluation of fabricated poly(L-lactic) acid core fibers for ligament fascicle development 用于韧带束发育的聚乳酸芯纤维的表征与评价

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117108

E. Laurilliard, K. L. Lee, J. A. Cooper

引用次数: 5

Force measurement across the patellofemoral joint using a smart patellar implant following a total knee arthroplasty 全膝关节置换术后使用智能髌骨植入物测量髌骨股骨关节的受力

2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC) Pub Date : 2015-04-17 DOI: 10.1109/NEBEC.2015.7117087

Matthew K. Dion, Colleen P. Healey, S. Giddings, John F. Drazan, Jared T Roberts, N. Cady, E. Ledet

引用次数: 2