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Resistance to antibiotics, metals, hydrophobicity and klebocinogeny of Klebsiella pneumoniae isolated from foods. 从食物中分离的肺炎克雷伯菌对抗生素、金属、疏水性和克雷伯菌的耐药性。
Cytobios Pub Date : 1999-01-01
J S Grewal, R P Tiwari
{"title":"Resistance to antibiotics, metals, hydrophobicity and klebocinogeny of Klebsiella pneumoniae isolated from foods.","authors":"J S Grewal,&nbsp;R P Tiwari","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Milk samples and milk products (69 in toto) were screened for the presence of Klebsiella pneumoniae (52%), and maximum isolations (77%) were from ice cream samples (13). The isolates were hydrophobic, non-haemolytic and possessed both mannose resistant (MR) and mannose sensitive (MS) pili or only MR pili when tested with human or sheep blood, respectively. All isolates were resistant to one metal at least whereas about 98% exhibited resistance to two or more metal ions. The resistance frequency of 93%, 90% and 66.7% was observed against silver (20 micrograms/ml), cadmium (20 micrograms/ml) and mercuric ions (20 micrograms/ml), respectively. Multiple drug resistance (MDR) was observed in 10% of the isolates only. A direct correlation between the metal ion and antibiotic resistance was found in MDR strains. The klebocin typeability of 53% and 61% was observed with 153-158 and 153-156, U-5 and U-6 groups, respectively. The most common typing patterns involved strains 424 (21%) and 442 (31.8%). Only 61% of the isolates showed enterotoxigenicity by the coagglutination test.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21335824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of ethyl methane sulphonate on biomass and protein production by Candida tropicalis. 甲烷磺酸乙酯对热带假丝酵母生物量和蛋白质产量的影响。
Cytobios Pub Date : 1999-01-01
Y A Mahmoud
{"title":"Effect of ethyl methane sulphonate on biomass and protein production by Candida tropicalis.","authors":"Y A Mahmoud","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Large doses of ethyl methanesulphonate (EMS) greatly increased the induction of auxotrophic mutants in Candida tropicalis. The maximum yield of biomass and protein was recorded in some mutants isolated after treatment with 60, 80 and 100 ppm EMS. The electrophoretic protein profile revealed typical banding patterns for both C. tropicalis wild type and mutants.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21440722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of tumour necrosis factor-alpha on total myofibrillar and sarcoplasmic protein synthesis and polysomal aggregation in rat skeletal muscles. 肿瘤坏死因子- α对大鼠骨骼肌总肌原纤维和肌浆蛋白合成及多体聚集的影响。
Cytobios Pub Date : 1999-01-01
I R Cheema, C Hermann, S Postell, B Holifield
{"title":"Effect of tumour necrosis factor-alpha on total myofibrillar and sarcoplasmic protein synthesis and polysomal aggregation in rat skeletal muscles.","authors":"I R Cheema,&nbsp;C Hermann,&nbsp;S Postell,&nbsp;B Holifield","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The total sarcoplasmic and myofibrillar protein synthesis was reduced in incubated fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus of rat after in vivo tumour necrosis factor-alpha treatment at 50 micrograms/kg/day for 5 days. The rate of protein synthesis in the myofibrillar fraction was inhibited more severely (41% in EDL and 34% in soleus) than that in the sarcoplasmic fraction (23% in EDL and 14% in soleus). Sucrose density gradient centrifugation analysis indicated that TNF-alpha treatment impaired polysomal aggregation in rat diaphragm muscle. Compared with the control muscles, the ratio of 40S and 60S subunits to polysomes was higher in TNF-alpha treated muscles. These findings suggest a role for TNF-alpha in the translational regulation of protein synthesis in rat skeletal muscle.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21441736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of fibroblast growth factor, insulin-like growth factor I, and transforming growth factor beta on satellite cell type I collagen expression and localization during differentiation. 成纤维细胞生长因子、胰岛素样生长因子I和转化生长因子β对卫星细胞I型胶原在分化过程中表达和定位的影响
Cytobios Pub Date : 1999-01-01
S G Velleman, D C McFarland
{"title":"Influence of fibroblast growth factor, insulin-like growth factor I, and transforming growth factor beta on satellite cell type I collagen expression and localization during differentiation.","authors":"S G Velleman,&nbsp;D C McFarland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Expression, and temporal and spatial distribution of type I collagen were investigated in chicken satellite cell cultures during differentiation. There was no difference in the relative amounts of type I collagen after treatment with basic fibroblast growth factor (FGF), insulin-like growth factor-I (IGF-I), or transforming growth factor beta 1 (TGF-beta 1). However, myotube morphology was influenced by the presence of the growth factors. The temporal and spatial distribution of type I collagen was also modified. Control cultures maintained a predominant distribution of type I collagen surrounding the cellular area until approximately 48 h after the initiation of fusion whereas cultures with FGF or IGF-I maintained a cellular localization of type I collagen throughout the fusion process. TGF-beta 1 resulted in the early formation of an extracellular network of type I collagen preceding control cultures by approximately 24 h. These results suggest that type I collagen expression but not localization is independent of satellite cell proliferation and differentiation.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21498758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of the butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on the arylamines N-acetyltransferase activity in rat white blood cells. 丁基羟基茴香醚(BHA)和丁基羟基甲苯(BHT)对大鼠白细胞芳胺n -乙酰转移酶活性的影响。
Cytobios Pub Date : 1999-01-01
H F Lu, H C Wu, W C Chang, J G Chung
{"title":"Effects of the butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on the arylamines N-acetyltransferase activity in rat white blood cells.","authors":"H F Lu,&nbsp;H C Wu,&nbsp;W C Chang,&nbsp;J G Chung","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) were used to determine any effects on the N-acetyltransferase (NAT) activity in rat whole blood and white blood cells as measured by high performance liquid chromatography assay for the amounts of N-acetyl-2-aminofluorene (AAF) and 2-aminofluorene (AF). Two assay systems were performed, one with cellular cytosols, the other with intact white blood cells. The NAT activity in the whole blood and white blood cell cytosols was suppressed by BHA and BHT in a dose-dependent manner, i.e. the higher the concentrations of BHA and BHT, the higher the inhibition of NAT activity. Time-course experiments showed that NAT activity measured from the intact white blood cells was inhibited by BHA and BHT up to 24 h. The results suggest that BHA and BHT suppressed AF acetylation in rat blood with intact white blood cells.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21553222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gypenoside induces apoptosis in human Hep3B and HA22T tumour cells. 绞股蓝皂苷诱导人Hep3B和HA22T肿瘤细胞凋亡。
Cytobios Pub Date : 1999-01-01
J C Chen, J G Chung, L D Chen
{"title":"Gypenoside induces apoptosis in human Hep3B and HA22T tumour cells.","authors":"J C Chen,&nbsp;J G Chung,&nbsp;L D Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of gypenoside, an active component of the Chinese herb Gynostemma pentaphyllum (Thumb) Makino, on human hepatoma cell lines (Hep3B and HA22T) was investigated. Results demonstrated that gypenoside inhibited the proliferation or viability of the Hep3B and HA22T cells in a dose-dependent manner. The Hep3B and HA22T cells treated with gypenoside for 2 days were less DNA stainable and formed a sub-G1 peak. The treated cells increased cell numbers in the A0 region as well as shifting the ordinary S phase to the final S phase (D1 region), and induced a ladder pattern of fragmented DNA of about 200 base pairs. These data suggest that the cell death of the hepatoma cell lines Hep3B and HA22T induced by gypenoside was via apoptosis, and this was confirmed by morphological studies.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21498878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro studies on the effects of some anthelmintics on Cotylophoron cotylophorum (Digenea, Paramphistomidae): a structural analysis. 几种驱虫剂对副蝗科子叶蝗体外作用的结构分析。
Cytobios Pub Date : 1999-01-01
L Veerakumari, N Munuswamy
{"title":"In vitro studies on the effects of some anthelmintics on Cotylophoron cotylophorum (Digenea, Paramphistomidae): a structural analysis.","authors":"L Veerakumari,&nbsp;N Munuswamy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of the anthelmintics praziquantel (PZQ), levamisole (LEV), mebendazole (MBZ), fenbendazole (FBZ) and albendazole (ABZ), on the morphology and the histology of a digenetic trematode, Cotylophoron cotylophorum, were studied. Scanning electron micrographs of the drug-treated worms revealed that PZQ was the most effective drug inducing surface damages to a great extent. The parasite exposed to PZQ for 6 h, showed smaller blebs on the oral sucker region as well as on the sensory papillae. These blebs enlarged in size after 24 h and ruptured after 30 h of exposure. The worms treated with LEV showed a few smaller blebs on the ventrolateral margin. In MBZ- and FBZ-treated worms the blebs appeared between the oral and genital sucker after 6 h of incubation. The changes were not apparent in the ABZ-treated worms.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21328919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The karyotype of the South American rodent Kunsia tomentosus (Lichtenstein, 1830). 南美啮齿动物毛齿昆鼠的核型(列支敦士登，1830)。
Cytobios Pub Date : 1999-01-01
J Andrades-Miranda, A P Nunes, L F Oliveira, M S Mattevi
{"title":"The karyotype of the South American rodent Kunsia tomentosus (Lichtenstein, 1830).","authors":"J Andrades-Miranda,&nbsp;A P Nunes,&nbsp;L F Oliveira,&nbsp;M S Mattevi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chromosomal analysis of Kunsia tomentosus showed a karyotype with 2n = 44, constituted by 21 pairs of acrocentric autosomes. The X chromosome was a median acrocentric, between pairs 3 and 4 in size, and the Y chromosome was a small acrocentric (between pairs 19 and 20). Five pairs with nucleolus organizer regions were located at the short arms. C-banding showed blocks of constitutive heterochromatin occurring in the centromeres of all autosomes and of the X chromosome. The Y chromosome was entirely heterochromatic. In order to identify possible homologies, karyotypes of Kunsia and Scapteromys, the phyletically related taxa, were compared. No autosome shared by either genus was found by G-band comparisons. The C-band patterns and those produced by Alu I, Mbo I, Rsa I and Hae III restriction endonucleases were also different. The results of FISH indicated a different composition of the telomeric regions of the chromosomes of both taxa, since in Scapteromys the probes hybridized in both telomeres, and in Kunsia this hybridization only occurred in one of the telomeres. These differences also occurred in the localization and number of nucleolus organizer regions.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21395015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spontaneous germ cell death by apoptosis in epididymis of the adult bat Artibeus lituratus. 成蝠附睾生殖细胞凋亡所致的自发性死亡。
Cytobios Pub Date : 1999-01-01
S R Taboga, R S de Souza, D C dos Santos, S M Oliani
{"title":"Spontaneous germ cell death by apoptosis in epididymis of the adult bat Artibeus lituratus.","authors":"S R Taboga,&nbsp;R S de Souza,&nbsp;D C dos Santos,&nbsp;S M Oliani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Many factors can lead cells to apoptosis during the various stages of cell life. This study was undertaken to characterize germ cell death in the epididymis of the adult Artibeus lituratus by histochemical and immunohistochemical techniques using light microscopy and transmission electron microscopy. The results showed that cells with a nuclear phenotype and ultrastructural characteristics of chromatin compaction were common in apoptosis. The Apoptag test confirmed that the suspected cells were apoptotic. It is suggested that immature germ cells, when released from the germinative epithelium, may be directed towards the epididymis instead of being disposed of in the testicle. Furthermore, intact immature cells can leave the testicle in the initial phases of apoptosis and complete this phenomenon in the epididymis.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21395017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Importance of the role of calcium in programmed cell death: a review. 钙在程序性细胞死亡中的重要作用综述
Cytobios Pub Date : 1999-01-01
P D Gupta, K Pushkala
{"title":"Importance of the role of calcium in programmed cell death: a review.","authors":"P D Gupta,&nbsp;K Pushkala","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Calcium plays an important role in physiological cell death processes such as terminal differentiation and apoptosis. Cell injury occurs when the intracellular calcium pool is disturbed, which in turn may lead to cell death. Calcium in calcium-dependent enzymes, transglutaminases, various proteases, phosphorylases and kinase, is involved in the process of cell death. Examples of such enzymes involved in cell death, and the role of calcium levels in regulation of these enzymes, are described and discussed.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21440721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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