绞股蓝皂苷诱导人Hep3B和HA22T肿瘤细胞凋亡。

Cytobios Pub Date : 1999-01-01
J C Chen, J G Chung, L D Chen
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引用次数: 0

摘要

本文研究了绞股蓝有效成分绞股蓝皂苷对人肝癌细胞株Hep3B和HA22T的影响。结果表明,绞股蓝皂苷抑制Hep3B和HA22T细胞的增殖或活力呈剂量依赖性。绞股蓝皂苷处理2天后,Hep3B和HA22T细胞DNA染色减少,形成亚g1峰。处理后的细胞增加了A0区的细胞数量,并将普通S期转移到最终S期(D1区),并诱导了约200个碱基对的DNA片段的阶梯模式。这些数据表明绞股蓝皂苷诱导肝癌细胞株Hep3B和HA22T细胞凋亡,并通过形态学研究证实了这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gypenoside induces apoptosis in human Hep3B and HA22T tumour cells.

The effect of gypenoside, an active component of the Chinese herb Gynostemma pentaphyllum (Thumb) Makino, on human hepatoma cell lines (Hep3B and HA22T) was investigated. Results demonstrated that gypenoside inhibited the proliferation or viability of the Hep3B and HA22T cells in a dose-dependent manner. The Hep3B and HA22T cells treated with gypenoside for 2 days were less DNA stainable and formed a sub-G1 peak. The treated cells increased cell numbers in the A0 region as well as shifting the ordinary S phase to the final S phase (D1 region), and induced a ladder pattern of fragmented DNA of about 200 base pairs. These data suggest that the cell death of the hepatoma cell lines Hep3B and HA22T induced by gypenoside was via apoptosis, and this was confirmed by morphological studies.

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