Yiyue Zhang, Chao Zhuang, Can Tang, Qi-Ling Dou, Xiu‐Ju Luo, Xiu‐Ju Luo, Jun Peng
{"title":"Li Qi Huo Xue Di Wan Attenuates Cardiac and Lung Injury in Mice Exposed to Hypoxia Through Suppression of Apoptosis","authors":"Yiyue Zhang, Chao Zhuang, Can Tang, Qi-Ling Dou, Xiu‐Ju Luo, Xiu‐Ju Luo, Jun Peng","doi":"10.2174/0122150838341550250121114736","DOIUrl":"https://doi.org/10.2174/0122150838341550250121114736","url":null,"abstract":"Background: Li Qi Huo Xue Di Wan (LQHXDW), a traditional Chinese medicine, is used to treat patients with the symptoms of palpitations, chest tightness, chest pain, and shortness of breath. It is not known, however, whether LQHXDW can reduce high-altitude hypoxia-induced cardiopulmonary injury, what are the specific active ingredients, and what is the exact mechanism behind its cardiopulmonary protection. Objectives: This study intends to investigate the effect of LQHXDW on hypoxia-induced cardiopulmonary injury and the underlying mechanisms. Methods: The components of LQHXDW were identified by UPLC-QTOF-MS. The potential targets of LQHXDW against high-altitude hypoxia were screened via network pharmacology. Mice were subjected to an animal hypoxic chamber for 5 days to establish a high-altitude hypoxia animal model. Rat heart-derived H9c2 cells and human pulmonary artery endothelial cells (HPAECs) were cultured in glucose-free medium under hypoxic conditions (O2 /N2 /CO2, 3/92/5) for 72 h to mimic the high-altitude hypoxia in vivo. Results: The study found that LQHXDW can target apoptosis and inflammation pathways under the condition of high-altitude hypoxia . In mice exposed to hypoxia, LQHXDW reduced cardiac and lung injury, decreased inflammatory responses, and improved cardiac function. In vitro, LQHXDW increased cell viability and reduced apoptosis in cardiomyocytes and pulmonary artery endothelial cells. It also inhibited the activities of caspase-8 and caspase-3, preventing cardiopulmonary apoptosis. Discussion: The study investigates the impact of LQHXDW on hypoxia-induced heart and lung injury using UPLC-QTOF-MS and animal and cell models. Results show that LQHXDW can attenuate injury, prevent apoptosis, and protect the heart and lungs from hypoxia injury. It also demonstrates anti-inflammatory activity, suggesting LQHXDW as a potential candidate for traditional Chinese medicine in high-altitude sickness treatment. Conclusion: LQHXDW can be used as a potential inhibitor of apoptosis for treating high-altitude hypoxia. This study provided a clue for future studies to identify the exact active components of LQHXDW for targeting the pathways of apoptosis.","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ping Chen, Baibai Ye, Cheng Lin, Jia Chen, Chunping Xia, Linfu Li
{"title":"An Overview of Traditional Chinese Medicine Nursing for Patients with COVID-19","authors":"Ping Chen, Baibai Ye, Cheng Lin, Jia Chen, Chunping Xia, Linfu Li","doi":"10.2174/0122150838304552241129094342","DOIUrl":"https://doi.org/10.2174/0122150838304552241129094342","url":null,"abstract":"COVID-19 is a severe challenge facing the world today and poses a serious threat to the global public health security. Traditional Chinese medicine (TCM) nursing has played an active role in the treatment and prevention of COVID-19. This study investigated the role of the TCM in the nursing of COVID-19 patients, aiming to provide a comprehensive understanding of its effectiveness and application. This article conducted a systematic review of recent studies and clinical cases, analyzed the theoretical basis of TCM nursing for COVID-19, explored the role of TCM nursing in COVID-19 prevention and control from multiple perspectives, such as Chinese medicine treatment, acupuncture and massage treatment, and clinical nursing. Findings indicated that TCM nursing interventions significantly improved patient recovery rates, reduced symptoms, and enhanced overall well-being. In TCM clinical practice, clearing heat, herbs resolving dampness, and relieving exterior symptoms are commonly used. These are complemented by phlegm-resolving, Qi-regulating, and purgative herbs. Acupoints that can serve to support the body's righteousness and attempt to eliminate evil are very common.. Massage should mainly affect the lung meridian and its interior counterpart, the large intestine meridian, as well as regulate the Zhongjiao and overall body condition through the spleen, stomach, and Ren-Du meridians. Clinical nursing should not only address symptoms closely related to COVID-19, such as fever and respiratory issues, but also consider digestion, sleep, and emotions of the patient. TCM nursing provided a valuable complement to conventional treatments for COVID-19, emphasizing the need for further integration of TCM practices in global health strategies.","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"11 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Huang, Chong Xie, Zhi Quan Pan, Mengya Lin, Qingsheng Chen, Shengzhao Gong, Shengzhao Gong, Yadong Huang
{"title":"Exploring the Anti-melanogenic, Antioxidant, and Anti-inflammatory Activities of a Composition: Glabridin, Resveratrol and Ellagic Acid","authors":"Jie Huang, Chong Xie, Zhi Quan Pan, Mengya Lin, Qingsheng Chen, Shengzhao Gong, Shengzhao Gong, Yadong Huang","doi":"10.2174/0122150838296794240610130259","DOIUrl":"https://doi.org/10.2174/0122150838296794240610130259","url":null,"abstract":"Background: Plant extracts have wide applications in food, nutrition, and cosmetics, which results in a deeper investigation of natural ingredients. Numerous natural ingredients have been demonstrated to exhibit multiple activities, including antioxidation, anti-inflammation, and anti-melanogenesis. However, their combinations have not been well investigated, which could provide stronger performance with less toxicity and easier applications. Methods: We used B16F10 cells treated with alpha-melanocyte stimulating hormone (αMSH) for melanogenesis-related studies, including cellular melanin content, tyrosinase activity, and gene or protein expression. MTT assay was used to evaluate cell viability. DPPH scavenging activity was measured for antioxidation. Nitric oxide (NO) content was evaluated in lipopolysaccharide (LPS) treated RAW264.7 cells to indicate the performance on anti-inflammation. Results: In this study, six different compounds and their combinations were tested for melanogenesis. The results showed that the combination of glabridin, resveratrol, and ellagic acid (GRE) exhibited the highest efficiency, which was mainly manifested as inhibition of melanin production and tyrosinase activity, higher DPPH scavenging rate, and inhibition of nitric oxide (NO) production. Meanwhile, our results showed that GRE could significantly downregulate the expression of microphthalmia-associated transcription factor (MITF) related genes and proteins and could also inhibit the phosphorylation of cyclic AMP response element-binding protein (CREB), which was the upstream signal of MITF. Conclusion: The results suggest GRE exhibits high efficiency in inhibiting anti-melanogenesis, antioxidation, and anti-inflammation. Furthermore, GRE could downregulate the phosphorylation of the CREB and MITF signal pathway, which provides a theoretical basis for its application in pigmentation disorder disease and cosmetics.","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"11 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Yinxieling Inhibits Keratin 17 to Enhance Mitochondrial Function and Ameliorates Psoriasis-like Lesions","authors":"Shilei Wang, Helin Pan, Chenxi Zhao, Wanqun Chen, Jinwei Zhang","doi":"10.2174/0122150838312001241002094020","DOIUrl":"https://doi.org/10.2174/0122150838312001241002094020","url":null,"abstract":"Background: Psoriasis is a chronic skin disease characterized by erythema, scaling, and thickening. Yinxieling is a representative prescription for treating psoriasis developed by Professor Guowei Xuan, a master of traditional Chinese medicine. Keratin 17 is a key biomarker for the diagnosis and treatment of psoriasis, and its overexpression has been shown to impair mitochondrial function. Objectives: This study investigates the mechanism of action of Yinxieling in the treatment of psoriasis. Methods: In vivo, BALB/c mice were induced by imiquimod to create psoriasis-like mice model. Different doses of Yinxieling were administered to the psoriasis-like mice and PASI scores, thickness of epidermis and Keratin 17 expression of psoriasis-like mice were measured. In vitro, Ha- CaT cells were induced by IL-17A, IL-22 and TNF-alpha to establish psoriasis-like cells model. Yinxieling with different concentrations was administered to the psoriasis-like cells and Keratin 17 expression, adenosine triphosphate levels, and oxygen consumption rates were measured. Furthermore, the psoriasis-like cells were transfected with keratin 17 plasmids. Yinxieling intervened in these psoriasis-like cells with continuous high expression of keratin 17 and adenosine triphosphate levels and oxygen consumption rates were measured. Results: Yinxieling significantly improved PASI scores and decreased their epidermal thickness in psoriasis-like mice; Yinxieling also inhibited Krt17 expression in psoriasis-like mice and cells. Yinxieling improved ATP levels and oxygen consumption rate in psoriasis-like cells, and this effect of Yinxieling was abolished when the psoriasis-like cells were transfected with keratin 17 plasmids. Discussion: These findings suggest that Yinxieling alleviates psoriatic inflammation and improves mitochondrial function by downregulating KRT17. The study provides new mechanistic insights into the traditional use of Yinxieling and underscores the link between keratin dysregulation and mitochondrial impairment in psoriatic pathology. Conclusion: Yinxieling inhibits keratin 17 to enhance mitochondrial function and ameliorates psoriasis- like lesions.","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Impact of Carrageenan on Pharmascience","authors":"Akanksha Bhatt, Nidhi Nainwal, Priyank Purohit","doi":"10.2174/0122150838266638231117180516","DOIUrl":"https://doi.org/10.2174/0122150838266638231117180516","url":null,"abstract":"\u0000\u0000Carrageenan (CG) a sulfated polysaccharide (SP) is produced using a variety of seaweeds from the Rhodophyceae family. This type of seaweed is available in areas, like the Atlantic Ocean close to Great Britain, Europe, and North America. Carrageenan has been permitted\u0000for the use as food items with the European additive E-number E407. Carrageenan is a widely\u0000used polysaccharide derived from red seaweed and is known for its various applications in the\u0000chemical, biological, and pharmaceutical fields. It delves into its versatile applications across\u0000various sub areas, spanning from the food to the pharmaceutical industry. A significant emphasis\u0000is placed on the intricate roles of carrageenan in pharmaceutical science, where it serves as both\u0000a drug carrier agent and an active ingredient, owing to its noteworthy biological activity. This\u0000review aims to provide a comprehensive overview of carrageenan`s versatile applications, with a\u0000focus on its chemical properties, biological activities, and pharmaceutical uses. The\u0000pharmaceutical applications of carrageenan are further categorized into various subparts,\u0000including its role in treating diseases and its use in drug delivery systems, such as topical, oral,\u0000nasal, and unconventional routes. The review also incorporates the most recent developments in\u0000clinical trials involving carrageenan and its updated applications, drawing from authoritative\u0000sources. This comprehensive analysis aims to offer readers a clear understanding of\u0000carrageenan's multifaceted nature and its evolving significance in diverse industries.\u0000","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"12 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139438698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Kwape, Kabo Masisi, Laone Kelaotswe, Keagile Bati, Phazha Baeti
{"title":"Pro-oxidant Effects of Repeatedly Heated Sunflower Oil and the Possible\u0000Ameliorative Potential of Elaeodendron transvaalense Leaf Powder in\u0000Sprague Dawley Rats","authors":"T. Kwape, Kabo Masisi, Laone Kelaotswe, Keagile Bati, Phazha Baeti","doi":"10.2174/0122150838268705230928080148","DOIUrl":"https://doi.org/10.2174/0122150838268705230928080148","url":null,"abstract":"\u0000\u0000Repeated heating of cooking oils induces the overproduction of reactive\u0000oxygen species with an overwhelmed cellular antioxidant defense system, resulting in oxidative\u0000stress, the known cause of cardiovascular diseases. Elaeodendron transvaalense is a medicinal\u0000plant believed to have phytochemicals that help ameliorate the effects of oxidative stress.\u0000\u0000\u0000\u0000This study investigated the pro-oxidant effects of repeatedly heated sunflower oil and\u0000the possible ameliorative potential of Elaeodendron transvaalense leaf powder in Sprague Dawley\u0000rats.\u0000\u0000\u0000\u0000Four groups of male Sprague Dawley rats were fed for 4-weeks a standard diet supplemented\u0000without (a) control or with (b) 15% (w/w) repeatedly heated sunflower oil (RHSO),\u0000(c) 15% (w/w) non-heated sunflower oil, (d) 15% (w/w) RHSO and 1.5% ETLP.\u0000\u0000\u0000\u0000Endogenous antioxidants: Catalase and SOD activities were observed to rise significantly\u0000(p <0.05) in Group 2 when compared to Group 1 and Group 3 respectively. A significant\u0000decline in the SOD and catalase activities was observed in group 4 fed RHSO + ETLP. No significant\u0000differences were observed in the body, liver, and kidney weights of Group 2 when compared\u0000with Groups 1, 3 and 4 respectively, and no significant differences were observed when\u0000comparing Group 4 to Groups 1, 2 and 3. The levels of Lipid profiles; Low-density Lipoproteins\u0000(LDL), Total cholesterol (TC), and Triglycerides (TG) were observed to rise significantly (p\u0000<0.05) in Group 2 when compared to Group 1 and 3, while their significant (p <0.05) decline\u0000was observed in Group 4 fed with RHSO + ETLP. The levels of HDL showed no significant difference\u0000across all groups because the p-values for the difference between groups exceeded the\u0000null hypothesis (p <0.05).\u0000\u0000\u0000\u0000The findings of this experiment indicate the possible ameliorative potential of\u0000ETLP observed in the significantly (p <0.05) reduced SOD and catalase activities. A significant\u0000(p <0.05) decline in the TC, TG, and LDL to desirable levels further indicated the ameliorative\u0000effects.\u0000","PeriodicalId":11026,"journal":{"name":"Current Traditional Medicine","volume":"89 2","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139388153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}