Current Genetics最新文献_第4页

TOF1 and RRM3 reveal a link between gene silencing and the pausing of replication forks. TOF1和RRM3揭示了基因沉默和复制分叉暂停之间的联系。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-12-01 Epub Date: 2023-06-22 DOI: 10.1007/s00294-023-01273-3

Kholoud Shaban, Andrew Dolson, Ashley Fisher, Emma Lessard, Safia Mahabub Sauty, Krassimir Yankulov

引用次数: 0

Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1. Ino2是酵母磷脂生物合成基因的激活剂，与基础转录因子TFIIA和Bdf1相互作用。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-12-01 Epub Date: 2023-11-10 DOI: 10.1007/s00294-023-01277-z

Maike Engelhardt, Stefan Hintze, Eva-Carina Wendegatz, Julia Lettow, Hans-Joachim Schüller

{"title":"Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1.","authors":"Maike Engelhardt, Stefan Hintze, Eva-Carina Wendegatz, Julia Lettow, Hans-Joachim Schüller","doi":"10.1007/s00294-023-01277-z","DOIUrl":"10.1007/s00294-023-01277-z","url":null,"abstract":"Binding of general transcription factors TFIID and TFIIA to basal promoters is rate-limiting for transcriptional initiation of eukaryotic protein-coding genes. Consequently, activator proteins interacting with subunits of TFIID and/or TFIIA can drastically increase the rate of initiation events. Yeast transcriptional activator Ino2 interacts with several Taf subunits of TFIID, among them the multifunctional Taf1 protein. In contrast to mammalian Taf1, yeast Taf1 lacks bromodomains which are instead encoded by separate proteins Bdf1 and Bdf2. In this work, we show that Bdf1 not only binds to acetylated histone H4 but can also be recruited by Ino2 and unrelated activators such as Gal4, Rap1, Leu3 and Flo8. An activator-binding domain was mapped in the N-terminus of Bdf1. Subunits Toa1 and Toa2 of yeast TFIIA directly contact sequences of basal promoters and TFIID subunit TBP but may also mediate the influence of activators. Indeed, Ino2 efficiently binds to two separate structural domains of Toa1, specifically with its N-terminal four-helix bundle structure required for dimerization with Toa2 and its C-terminal β-barrel domain contacting TBP and sequences of the TATA element. These findings complete the functional analysis of yeast general transcription factors Bdf1 and Toa1 and identify them as targets of activator proteins.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"289-300"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of human BRCA2 in Saccharomyces cerevisiae complements the loss of RAD52 in double-strand break repair. 人BRCA2在酿酒酵母中的表达补充了RAD52在双链断裂修复中的损失。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-12-01 Epub Date: 2023-11-07 DOI: 10.1007/s00294-023-01278-y

Sherrice Law, Hannah Park, Eyar Shany, Sumer Sandhu, Mayukha Vallabhaneni, Damon Meyer

{"title":"Expression of human BRCA2 in Saccharomyces cerevisiae complements the loss of RAD52 in double-strand break repair.","authors":"Sherrice Law, Hannah Park, Eyar Shany, Sumer Sandhu, Mayukha Vallabhaneni, Damon Meyer","doi":"10.1007/s00294-023-01278-y","DOIUrl":"10.1007/s00294-023-01278-y","url":null,"abstract":"BRCA2 is a tumor-suppressor gene that is normally expressed in the breast and ovarian tissue of mammals. The BRCA2 protein mediates the repair of double-strand breaks (DSBs) using homologous recombination, which is a conserved pathway in eukaryotes. Women who express missense mutations in the BRCA2 gene are predisposed to an elevated lifetime risk for both breast cancer and ovarian cancer. In the present study, the efficiency of human BRCA2 (hBRCA2) in DSB repair was investigated in the budding yeast Saccharomyces cerevisiae. While budding yeast does not possess a true BRCA2 homolog, they have a potential functional homolog known as Rad52, which is an essential repair protein involved in mediating homologous recombination using the same mechanism as BRCA2 in humans. Therefore, to examine the functional overlap between Rad52 in yeast and hBRCA2, we expressed the wild-type hBRCA2 gene in budding yeast with or without Rad52 and monitored ionizing radiation resistance and DSB repair efficiency. We found that the expression of hBRCA2 in rad52 mutants increases both radiation resistance and DSB repair frequency compared to cells not expressing BRCA2. Specifically, BRCA2 improved the protection against ionizing radiation by at least 1.93-fold and the repair frequency by 6.1-fold. In addition, our results show that homology length influences repair efficiency in rad52 mutant cells, which impacts BRCA2 mediated repair of DSBs. This study provides evidence that S. cerevisiae could be used to monitor BRCA2 function, which can help in understanding the genetic consequences of BRCA2 variants and how they may contribute to cancer progression.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"301-308"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amyloids and prions in the light of evolution. 从进化角度看淀粉样蛋白和朊病毒。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-12-01 Epub Date: 2023-05-10 DOI: 10.1007/s00294-023-01270-6

Alexey P Galkin, Evgeniy I Sysoev, Anna A Valina

{"title":"Amyloids and prions in the light of evolution.","authors":"Alexey P Galkin, Evgeniy I Sysoev, Anna A Valina","doi":"10.1007/s00294-023-01270-6","DOIUrl":"10.1007/s00294-023-01270-6","url":null,"abstract":"Functional amyloids have been identified in a wide variety of organisms including bacteria, fungi, plants, and vertebrates. Intracellular and extracellular amyloid fibrils of different proteins perform storage, protective, structural, and regulatory functions. The structural organization of amyloid fibrils determines their unique physical and biochemical properties. The formation of these fibrillar structures can provide adaptive advantages that are picked up by natural selection. Despite the great interest in functional and pathological amyloids, questions about the conservatism of the amyloid properties of proteins and the regularities in the appearance of these fibrillar structures in evolution remain almost unexplored. Using bioinformatics approaches and summarizing the data published previously, we have shown that amyloid fibrils performing similar functions in different organisms have been arising repeatedly and independently in the course of evolution. On the other hand, we show that the amyloid properties of a number of bacterial and eukaryotic proteins are evolutionarily conserved. We also discuss the role of protein-based inheritance in the evolution of microorganisms. Considering that missense mutations and the emergence of prions cause the same consequences, we propose the concept that the formation of prions, similarly to mutations, generally causes a negative effect, although it can also lead to adaptations in rare cases. In general, our analysis revealed certain patterns in the emergence and spread of amyloid fibrillar structures in the course of evolution.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"189-202"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9443067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Romberg Neck Torsion :A New Specific Test for Cervicogenic Dizziness. Romberg颈部扭转:一种新的颈源性头晕特异性检测方法。

IF 1.8 4区生物学

Current Genetics Pub Date : 2023-12-01 Epub Date: 2023-06-02 DOI: 10.1007/s12070-023-03902-2

Soheil Mansour Sohani, Mehdi Akbari, Morteza Hamidi Nahrani

{"title":"Romberg Neck Torsion :A New Specific Test for Cervicogenic Dizziness.","authors":"Soheil Mansour Sohani, Mehdi Akbari, Morteza Hamidi Nahrani","doi":"10.1007/s12070-023-03902-2","DOIUrl":"10.1007/s12070-023-03902-2","url":null,"abstract":"Aims: Previous studies have shown that Cervical myofascial pain syndrome with dizziness (CMPS-D ) is one of the most common causes of cervicogenic dizziness and is associated with challenge in diagnosis and treatment. this study aimed to investigate the Romberg neck torsion test in patients with CMPS-D and healthy controls.Materials and methods: Cross-sectional, observational study. twenty patients with CMPS-D were compared with twenty healthy controls. the Romberg (neutral position and neck torsion) and smooth pursuit neck torsion tests were performed in patients with CMPS-D and healthy controls.Results: The results confirmed that there are significant differences in the Romberg neck torsion test between subjects with CMPS-D and healthy controls (p < 0.05). in addition, There was a significant correlation between Romberg neck torsion and smooth pursuit neck torsion results (p < 0.05).Conclusion: The results of Romberg neck torsion test in CMPS-D subjects were different from those in healthy controls, which was attributed to neck pain and changes in cervical proprioception input. Romberg neck torsion is a new method for assessing cervicogenic dizziness.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"11 1","pages":"2960-2965"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72764467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria. 伊曲康唑对假丝酵母的抗真菌作用很大程度上取决于线粒体产生的氧化应激。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01269-z

Mª Luz Muñoz-Megías, Ruth Sánchez-Fresneda, Francisco Solano, Sergi Maicas, María Martínez-Esparza, Juan-Carlos Argüelles

{"title":"The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria.","authors":"Mª Luz Muñoz-Megías, Ruth Sánchez-Fresneda, Francisco Solano, Sergi Maicas, María Martínez-Esparza, Juan-Carlos Argüelles","doi":"10.1007/s00294-023-01269-z","DOIUrl":"https://doi.org/10.1007/s00294-023-01269-z","url":null,"abstract":"In Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-exposed cells. Analysis of the antioxidant enzymatic activities of catalase and superoxide dismutase pointed to a moderate decrease of catalase in trehalase-deficient mutant cells compared to the wild type, with an additional increase upon addition of rotenone. These enzymatic changes were imperceptible in the case of superoxide dismutase. Alternative assays with Voriconazole led to a similar profile in the results regarding cell growth and antioxidant activities. Collectively, our data suggest that the antifungal action of Itraconazole on C. parapsilosis is dependent on a functional mitochondrial activity. They also suggest that the central metabolic pathways in pathogenic fungi should be considered as preferential antifungal targets in new research.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"165-173"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10039597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans. 在芽曲霉中，ChsB的n端紊乱区调节其向菌丝顶端表面的有效运输。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01267-1

Jingyun Jin, Ryo Iwama, Hiroyuki Horiuchi

{"title":"The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans.","authors":"Jingyun Jin, Ryo Iwama, Hiroyuki Horiuchi","doi":"10.1007/s00294-023-01267-1","DOIUrl":"https://doi.org/10.1007/s00294-023-01267-1","url":null,"abstract":"In fungi, the cell wall plays a crucial role in morphogenesis and response to stress from the external environment. Chitin is one of the main cell wall components in many filamentous fungi. In Aspergillus nidulans, a class III chitin synthase ChsB plays a pivotal role in hyphal extension and morphogenesis. However, little is known about post-translational modifications of ChsB and their functional impacts. In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-terminal disordered region.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"175-188"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1. 酵母多效性共抑制因子Sin3、Cyc8和Tup1相互作用的基因抑制介导域功能表征及比较分析。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01262-6

Julia Lettow, Felix Kliewe, Rasha Aref, Hans-Joachim Schüller

{"title":"Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.","authors":"Julia Lettow, Felix Kliewe, Rasha Aref, Hans-Joachim Schüller","doi":"10.1007/s00294-023-01262-6","DOIUrl":"https://doi.org/10.1007/s00294-023-01262-6","url":null,"abstract":"Transcriptional corepressors Sin3, Cyc8 and Tup1 are important for downregulation of gene expression by recruiting various histone deacetylases once they gain access to defined genomic locations by interaction with pathway-specific repressor proteins. In this work we systematically investigated whether 17 yeast repressor proteins (Cti6, Dal80, Fkh1, Gal80, Mig1, Mot3, Nrg1, Opi1, Rdr1, Rox1, Sko1, Ume6, Ure2, Xbp1, Yhp1, Yox1 and Whi5) representing several unrelated regulatory pathways are able to bind to Sin3, Cyc8 and Tup1. Our results show that paired amphipathic helices 1 and 2 (PAH1 and PAH2) of Sin3 are functionally redundant for some regulatory pathways. WD40 domains of Tup1 proved to be sufficient for interaction with repressor proteins. Using length variants of selected repressors, we mapped corepressor interaction domains (CIDs) in vitro and assayed gene repression in vivo. Systematic comparison of CID minimal sequences allowed us to define several related positional patterns of hydrophobic amino acids some of which could be confirmed as functionally supported by site-directed mutagenesis. Although structural predictions indicated that certain CIDs may be α-helical, most repression domains appear to be randomly structured and must be considered as intrinsically disordered regions (IDR) adopting a defined conformation only by interaction with a corepressor.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"127-139"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9682479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe. 转录延伸因子TFIIS对裂糖酵母pka1缺失对染色体分离的影响。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01268-0

Kouhei Takenaka, Shiho Nishioka, Yuki Nishida, Makoto Kawamukai, Yasuhiro Matsuo

{"title":"Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe.","authors":"Kouhei Takenaka, Shiho Nishioka, Yuki Nishida, Makoto Kawamukai, Yasuhiro Matsuo","doi":"10.1007/s00294-023-01268-0","DOIUrl":"https://doi.org/10.1007/s00294-023-01268-0","url":null,"abstract":"The cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rst2∆, tfs1∆, mca1∆, and moc3∆ suppressed the TBZ-sensitive phenotype of the pka1∆ strain, among which tfs1∆ was the strongest suppressor. All single mutants (rst2∆, tfs1∆, mca1∆, and moc3∆) showed a TBZ-tolerant phenotype. Tfs1 has two transcriptional domains (TFIIS and Zn finger domains), both of which contributed to the suppression of the pka1∆-induced TBZ-sensitive phenotype. pka1∆-induced chromosome mis-segregation was rescued by tfs1∆ in the presence of TBZ. tfs1 overexpression induced the TBZ-sensitive phenotype and a high frequency of chromosome mis-segregation, suggesting that the amount of Tfs1 must be strictly controlled. However, Tfs1-expression levels did not differ between the wild-type and pka1∆ strains, and the Tfs1-GFP protein was localized to the nucleus and cytoplasm in both strains, which excludes the direct regulation of expression and localization of Tfs1 by Pka1. Growth inhibition by TBZ in pka1∆ strains was notably rescued by double deletion of rst2 and tfs1 rather than single deletion of rst2 or tfs1, indicating that Rst2 and Tfs1 contribute independently to counteract TBZ toxicity. Our findings highlight Tfs1 as a key transcription factor for proper chromosome segregation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"115-125"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9420607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1. 麦角菌组蛋白甲基转移酶 Set1 对基因调控的多样动态形式

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-03-31 DOI: 10.1007/s00294-023-01265-3

Neha Deshpande, Mary Bryk

{"title":"Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1.","authors":"Neha Deshpande, Mary Bryk","doi":"10.1007/s00294-023-01265-3","DOIUrl":"10.1007/s00294-023-01265-3","url":null,"abstract":"Gene transcription is an essential and highly regulated process. In eukaryotic cells, the structural organization of nucleosomes with DNA wrapped around histone proteins impedes transcription. Chromatin remodelers, transcription factors, co-activators, and histone-modifying enzymes work together to make DNA accessible to RNA polymerase. Histone lysine methylation can positively or negatively regulate gene transcription. Methylation of histone 3 lysine 4 by SET-domain-containing proteins is evolutionarily conserved from yeast to humans. In higher eukaryotes, mutations in SET-domain proteins are associated with defects in the development and segmentation of embryos, skeletal and muscle development, and diseases, including several leukemias. Since histone methyltransferases are evolutionarily conserved, the mechanisms of gene regulation mediated by these enzymes are also conserved. Budding yeast Saccharomyces cerevisiae is an excellent model system to study the impact of histone 3 lysine 4 (H3K4) methylation on eukaryotic gene regulation. Unlike larger eukaryotes, yeast cells have only one enzyme that catalyzes H3K4 methylation, Set1. In this review, we summarize current knowledge about the impact of Set1-catalyzed H3K4 methylation on gene transcription in S. cerevisiae. We describe the COMPASS complex, factors that influence H3K4 methylation, and the roles of Set1 in gene silencing at telomeres and heterochromatin, as well as repression and activation at euchromatic loci. We also discuss proteins that \"read\" H3K4 methyl marks to regulate transcription and summarize alternate functions for Set1 beyond H3K4 methylation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"91-114"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9442451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0